1,720,960 research outputs found
Diagnosis of Ascaris sp.in Human by Using Molecular Technique
No full textThis study aimed to Diagnose of Ascaris sp.in human by using molecular technique. Microscopically looking for Ascaris sp. eggs, human faeces were collected from 120 patients admitted to private clinic in Baghdad, Iraq. The Kato-Katz method (Hell-Tec kit) was used for the parasitological diagnosis. DNA Stool Mini kit (Qiagen) was used to process 200 l of material, although with some adjustments (Proteinase K digestion at 55-60 °C for 4 h). To determine whether typical PCR inhibitors found in faeces were present, human DNA PCR was carried out. The cytb gene and ITS1 (480 bp) were used for molecular detection of this parasite. The genotypes of Ascaris need to be amplified and identified. The most reliable method for diagnosing Ascaris intestinal infections is the detection of parasite eggs in faecal samples using a light microscope. The percentage of Ascaris spp. by microscopical method was 2.5 %. The molecular results showed that cytb gene was showed in 16 samples, while ITS1 gene were showed in 18 samples.
In conclusion, the molecular method showed higher sensitivity in diagnosis of Ascaris spp. from human feces when compared with microscopical method
Serological Detection of Giardiasis in Children with Diarrhea
Get PDFThis study aimed for serological detection of giardiasis in children with diarrhea. The present study included the acquisition of 126 faecal specimens from a diverse cohort of children spanning all genders and age ranges. These samples were submitted to the laboratory located in Babylon province, Iraq, on many occasions over the period from 2020 to 2022. The gathered specimens were recorded in a tabular format, subsequently leading to the transportation of all faecal samples to the laboratory. Following this, a thorough examination of all samples was conducted using a light microscope, and any samples that raised concerns were subjected to further analysis employing a serological methodology. All samples that were predominantly recognised as G. lamblia in the laboratory were subjected to examination using a serological test. The serological test used an ELISA kit manufactured by Sanlong, and the instructions provided by the manufacturer were followed. The overall prevalence of G. lamblia infection in Babylon city was found to be 5.6%. Specifically, the infection rate among men was 4.1%, while among females it was 6.5%. In relation to the age cohorts, all participants under study were categorised into three distinct age groups. Notably, the age group including persons aged 6-10 years exhibited the greatest infection rate at 7.1%. Additionally, infection rates of 4.4% and 2.6% were seen in the age groups of 1-5 years and above 10 years, respectively. Infections were seen in faeces samples exhibiting semi-formed consistency, loose consistency, and watery consistency, as indicated by the stool types. The prevalence of infection was greater in the loose type.
In conclusion, the present cross-sectional investigation indicates that the incidence of giardiasis in Babylon city is very low. Based on the findings, it can be inferred that the incidence of G. lamblia infection among children is also low. Furthermore, no significant variations were observed in relation to stool type and gender. Nevertheless, it is crucial to implement targeted and appropriate health programmes for the prevention of this disease
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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