1,354,484 research outputs found
Giuseppe Aliprandi e la "raccolta Aliprandi" presso la Biblioteca Civica di Padova
No full textLa figura di Giuseppe Aliprandi, matematico e studioso di bibliografia della stenografia, e della sua biblioteca, donata alla Biblioteca Civica di Padova, sono l'oggetto di questo studio
Antonio Aliprandi, un estucador lombardo en la Valencia de 1700
No full textEn torno a 1700 aparecen en Valencia varios artistas extranjeros que residirán en la ciudad varios años dejando en ella un considerable volumen de obras. Uno de ellos, Antonio Aliprandi, se especializará en la factura de revestimientos de estuco para interiores principalmente eclesiásticos. En el presente texto se realiza un recorrido por las obras de Aliprandi en Valencia y se documentan varias de ellas hasta el momento sólo atribuidas a su mano.
Around 1700 many foreign artists appear in Valencia where they will stand for several years leaving in our town quite a lot of handcrafts. One among them, Antonio Aliprandi, will specialize himself on recovering many buildings with stucco, mainly in churches. This text makes a journey along Aliprandi's art pieces in Valencia after working on files to find the documents refered to them
Multinuclear PtII Complexes : Why Three is Better Than Two to Enhance Photophysical Properties
No full textThe self-assembly of platinum complexes is a well-documented process that leads to interesting changes of the photophysical and electrochemical behavior as well as to a change in reactivity of the complexes. However, it is still not clear how many metal units must interact in order to achieve the desired properties of a large assembly. This work aimed to clarify the role of the number of interacting PtII units leading to an enhancement of the spectroscopic properties and how to address inter- versus intramolecular processes. Therefore, a series of neutral multinuclear PtII complexes were synthesized and characterized, and their photophysical properties at different concentration were studied. Going from the monomer to dimers, the growth of a new emission band and the enhancement of the emission properties were observed. Upon increasing the platinum units up to three, the monomeric blue emission could not be detected anymore and a concentration independent bright-yellow/orange emission, due to the establishment of intramolecular metallophilic interactions, was observed
Controlling and imaging biomimetic self-assembly
No full textThe self-assembly of chemical entities represents a very attractive way to create a large variety of ordered functional structures and complex matter. Although much effort has been devoted to the preparation of supramolecular nanostructures based on different chemical building blocks, an understanding of the mechanisms at play and the ability to monitor assembly processes and, in turn, control them are often elusive, which precludes a deep and comprehensive control of the final structures. Here the complex supramolecular landscape of a platinum(II) compound is characterized fully and controlled successfully through a combination of supramolecular and photochemical approaches. The supramolecular assemblies comprise two kinetic assemblies and their thermodynamic counterpart. The monitoring of the different emission properties of the aggregates, used as a fingerprint for each species, allows the real-time visualization of the evolving self-assemblies. The control of multiple supramolecular pathways will help the design of complex systems in and out of their thermodynamic equilibrium
UNRAVELLING THE ROLE OF NOVEL FACTORS INVOLVED IN DOUBLE-STRAND BREAK REPAIR.
No full textEach day every cell of a living organism is constantly exposed to numerous DNA damages deriving both from the environment but also from its own metabolism. The high number of lesions and the consequent genome instability make of DNA damages one of the weightiest challenge to face for a cell. Indeed the ability to detect, recognise and repair a lesion is of pivotal importance, since on these events depend the stability of the genome and, ultimately, cell viability. The main shield eukaryotic cells have evolved to face this challenge is the DNA damage response, a protein network that allow repair of the lesions. Human cells can rely on two main mechanisms to repair double strand breaks, one of the most harmful lesions: homologous recombination and non-homologous end joining. The correct balance between these two pathways depends on cell cycle, chromatin conformation and on the interplay among different factors. In addition, important for the correct pathway choice is the DNA end resection process. It consists in a nucleolytic degradation of the DSB ends to generate a 3' protruding tail to invade the homologous sequence, used as a template to accomplish the HR. Fine regulation of resection is particularly important to correctly repair the damage and prevent genome instability, fuel of cancer.
In this Thesis I present the work performed during my three years of PhD, in which I’ve been involved in two projects. Using human cells as a model system I’ve analysed the role of two different proteins, both involved in DNA repair pathway choice: DAXX and SLX4.
In the first and half year of my PhD, I analysed the effect of double strand break-dependent phosphorylation of DAXX on its activity as a chaperone of the histone variant H3.3. In brief, we found that upon double strand break, DAXX is phosphorylated by the apical kinase ATM on two serine (S424 S712) and the ability of DAXX to depose H3.3 at the lesion relies on these modifications. The accumulation and maintenance of H3.3 at the damage impact on the histone post-translational modification pattern, impairing 53BP1 protein foci formation and favouring the damage to be repaired through homologous recombination. Our results highlight the important role of histones chaperones and modifications in double strand break repair and suggest a possible mechanism explaining the prediatric glioblastoma occurrence in case of H3.3 mutations.
During the last part of my PhD I focused my attention of the role of SLX4 protein in double strand break repair pathway choice. Preliminary data of our laboratory suggested an SLX4 pro-resection activity, favouring homologous recombination occurrence. Staring from these results, I analysed resection in Fanconi Anemia patient-derived cells, SLX4 null. In collaboration with Pablo Huertas’ laboratory (CABIMER, Sevilla, Spain) I verified an impairment of the resection process in these cells, confirming SLX4 pro-resection role. Further analysis will be required to elucidate the molecular mechanism of SLX4 activity but these first results are very promising to shed light on a new player of the intricate network of double strand breaks repair pathway choice
Chiral Amplification by Self-Assembly of Neutral Luminescent Platinum(II) Complexes
No full textTwo novel enantiomerically pure chiral ligands and the corresponding neutral PtII complexes have been synthetized and characterized. The self-assembly properties of the complexes have been investigated using different morphological and photophysical techniques. The two enantiomeric complexes, 4 a and 4 b, show high tendency to self-assemble into chiral supramolecular aggregates with right (P) and left-handed (M) helical configurations, respectively, as proven by SEM and absorption circular dichroism. The formation of such organized structures is driven by the formation of metallophilic and π–π interactions between spatially close Pt complexes with an enhancement of the chiro-optical properties in the solid state
Chiral Amplification by Self-Assembly of Neutral Luminescent Platinum(II) Complexes
No full textTwo novel enantiomerically pure chiral ligands and the corresponding neutral PtII complexes have been synthetized and characterized. The self-assembly properties of the complexes have been investigated using different morphological and photophysical techniques. The two enantiomeric complexes, 4 a and 4 b, show high tendency to self-assemble into chiral supramolecular aggregates with right (P) and left-handed (M) helical configurations, respectively, as proven by SEM and absorption circular dichroism. The formation of such organized structures is driven by the formation of metallophilic and π–π interactions between spatially close Pt complexes with an enhancement of the chiro-optical properties in the solid state
Early combined arthroscopic treatment for simultaneous ruptures of the patellar tendon and the anterior cruciate ligament leads to good radiological results and patient satisfaction
No full textSimultaneous rupture of the patellar tendon (PT) and the anterior cruciate ligament (ACL) is a relatively rare injury. This study offers a comprehensive review of the published reports and presents two cases of simultaneous PT and ACL rupture
Aggregation-Induced Emission in Electrochemiluminescence: Advances and Perspectives
No full textThe discovery of aggregation-induced electrochemiluminescence (AIECL) in 2017 opened new research paths in the quest for novel, more efficient emitters and platforms for biological and environmental sensing applications. The great abundance of fluorophores presenting aggregation-induced emission in aqueous media renders AIECL a potentially powerful tool for future diagnostics. In the short time following this discovery, many scientists have found the phenomenon interesting, with research findings contributing to advances in the comprehension of the processes involved and in attempts to design new sensing platforms. Herein, we explore these advances and reflect on the future directions to take for the development of sensing devices based on AIECL. Graphic abstract: [Figure not available: see fulltext.]
Chronic epigastrich blood collection after coronary artery bypass grafting — case report.
No full textA clinical case of epigastric pain 3 months after coronary artery bypass grafting (CABG) is presented. The CT finding of an abdominal mass with thick wall, internal horizontal by-gravity level and linear contrast enhancement at the border between the two different components was correctly interpreted for a chronic hematoma with separation of the formed blood elements from blood serum; in particular, the linear contrast enhancement was related to an artery-feeding vessel
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