1,721,324 research outputs found
Species Identification and Food Fraud Analysis in Commercial Fish Products Through DNA- based methods
Full text linkGlobalization of food supply chains has led to an increased uncertainty of the origin and safety of fish-based products. Barcoding can be used to validate the labelling of products and to trace their origin. “Fish fraud” has been discovered across the globe. Barcoding can also trace fish species as there can be human health hazards related to consumption of fish. The study evaluated the applicability of the mitochondrial genes cytochrome b (cytB), and cytochrome oxidase subunit I (COI) for the identification of fish and processed fish product by DNA barcoding. In the study, universal primers for mitochondrial cytB were used to discriminate fish species in raw and processed forms. The barcode primers were cross tested against collected fish product. In this study DNA barcoding was employed to identify fishery product collected from market and supermarket located in Apulia region (Southern Italy). We collected and analyses 90 samples for our study. For this project different varieties of fish samples were collected from different supermarkets and of different companies. DNA was isolated from all samples and amplified by PCR; the most intense amplified product was chosen for Sanger Sequencing. After sequencing, the sequences were matched with NCBI BLAST and FISH BOL. After obtaining the results species were identified and matched with the labelling of the products. Non-compliance between the species detected and the species declared in the label was detected in 10 out of 90 (11.1) % samples. The study provides further evidence of the need for increased traceability and assessment of food products authentication. Additionally, correct species denomination and traceability may increase the standard of management of hazards related to fish and food safety as well as ensuring product authenticity, providing reliable information to consumers.
Another objective of the thesis was the development of loop-mediated isothermal amplification (LAMP) assay for rapid and direct screening of yellowfin tuna (Thunnus albacares) in commercial fish products. In this study, a loop mediated isothermal amplification (LAMP) assay was developed and validated targeting the mitochondrial cytochrome b gene for fast and direct detection of Thunnus albacares, which is a valuable species of tuna. The analytical specificity was confirmed using 18 target samples (Thunnus albacares) and 18 samples of non-target fish species. The analytical sensitivity of the LAMP assay was 540 fg DNA per reaction. In addition, a simple and direct swab method without
time-consuming nucleic acid extraction procedures and the necessity for cost-intensive laboratory equipment was performed that allowed LAMP detection of Thunnus albacares samples within 13 minutes. Due to its high specificity and sensitivity, the LAMP assay can be used as a rapid and on-site screening method for the identification of Thunnus albacares, potentially providing a valuable monitoring tool for food authenticity control by the authoritie
MENGGAGAS UNIVERSITAS ISLAM IDEAL: Studi Terhadap Pemikiran Syed Ali Ashraf
Full text link<p>Abstrak: Tujuan dari penelitian ini adalah untuk mengkaji tentang konsep universitas Islam menurut Ashraf. Penelitian ini adalah penelitian kualitatif di mana pengumpulan datanya dilakukan melalui studi kepustakaan dan datanya kemudian dianalisis dengan cara deskriptif. Idealnya, sebuah universitas Islam harus memiliki konsep yang luas tentang pendidikan Islam yang tidak membatasi dirinya hanya mengajarkan ilmu-ilmu keislaman semata, tetapi harus mencakup pengajaran semua subjek karena semuanya berdasarkan pada tauhid. Selanjutnya, sebuah universitas Islam seharusnya tidak hanya memberikan pengajaran dan pelatihan yang hanya mengisi otak para mahasiswanya saja tetapi lebih dari itu harus bertujuan untuk menghasilkan manusia yang tercerahkan dengan ilmu pengetahuan yang benar dan watak yang mulia. Selain itu, para mahasiswanya harus mampu bekerja untuk kesejahteraan umat manusia berdasarkan landasan spiritual. Sebuah universitas Islam idealnya juga harus memberikan prioritas untuk melakukan penelitian sehingga harus memiliki pusat penelitian yang baik untuk memfasilitasi para penelitinya yang berkualitas untuk semua cabang ilmu pengetahuan.</p><p><br />Abstract: Initiating an Ideal University: A Study of Syed Ali Ashraf’s Thought. The objective of this research is to study the concept of Islamic university according to Ashraf. This is a qualitative research whose data were collected through library research and using the method of descriptive analysis. An Islamic university should have a broad concept of Islamic education that does not limit itself only to give instruction of traditional Islamic sciences, but it should include all subjects since they are based on tawhid. Furthermore, an Islamic university should provide not only instruction and training to fulfill the mind of its students but more than that it should be aimed at producing enlightened people with sound knowledge and noble character. In addition, its students must be able to work for the welfare of human being on the basis of spirituality. It should also give priority to undertake research, therefore it must provide research center to facilitate qualified researches of all branches of knowledge.</p><p><br />Kata Kunci: pendidikan Islam, Universitas Islam, dualism, Syed Ali Ashraf</p></jats:p
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
An Investigation of the Pace of the Story Time in the Narrative of “Cloudy Years” Novel by Ali-Ashraf Darvishian
Full text link“Cloudy Years” novel by Ali-Ashraf Darvishian written in 4 volumes and 1622 pages and its events contain about 33 years of hero-narrator’s life. In the article, the speed of passing the story events through the author’s narrative is measured on the basis of Gerard Genette’s opinions. Genette utilizes order, duration, and frequency categories to measure the tempo of a novel. The study and survey of the existent specimens in all the novel demonstrate that the order of the events of the novel is more lineal, even though analepses and prolepses would be seen in the narrative, and evaluating the duration of the story and narrative makes clear that the average of 7/42 days of story exhibited in every page of the novel. The comparison between the average duration of every volume of the novel with the average duration of the novel proves negative, positive, positive and negative fluctuations in every volume of the novel, in turn. The acquired statistics of how being repeated the events of story approve also the more number of singulative frequencies which has a significant relationship with more lineal passing the narrative. The findings and conclusion of this research present new points in its theoretical framework
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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