164,958 research outputs found
Virus de la cebolla
No full textFil: Alcalde Lassalle, Alberto J..
Universidad Nacional de Cuyo. Facultad de Ciencias Agraria
Visita de trabajo del alcalde de Aledo, Juan J. Andreo
No full textVisita de trabajo del alcalde de Aledo, Juan J. Andreo a la UM. La reunión se celebró en el despacho de la Secretaría General con la presencia del rector José Orihuela Calatayud
[Report to Chief J. E. Curry, by an unknown author #1]
No full textReport to Chief J. E. Curry, by an unknown author. The report contains a list of officers who gave depositions to the United States Attorney
[Report to Chief J. E. Curry, by an unknown author #2]
No full textReport to Chief J. E. Curry, by an unknown author. The report contains a list of officers who gave depositions to the United States Attorney
Stop Mare Mortum y el movimiento de solidaridad con las personas refugiadas en Barcelona
No full textThe 2015 long summer of migration has increased dramatically the level of citizen awareness and activism in Barcelona. In Spring 2016 a number of protest events in solidarity with refugees were taking place on a daily basis, promoted by a broad range of local groups, associations and networks. In the meantime, a change of government brought a social activist as the new mayor of the city, with the refugees' issue as a top political priority. Based on a number of in-depth interviews with key activists, this article presents, maps and studies the evolution of the local networks. Aiming at shedding light on meso-level mobilization dynamics, we zoom into Stop Mare Mortum. With a high level of politicization and focusing on refugees in transit, this initiative borne out of a small network of activists has gradually become an umbrella platform aiming to coordinate civil society initiatives within this field. Together with a unique combination emotions and frames for mobilization, SMM's ability to adapt its strategies, repertoires of action and organizational structures to a changing environment explains its mobilization capacity and the cross-cut nature of its constituency
Visita de Trabajo del Alcalde de Aledo, Juan J. Andreo. Julio 2015
No full textVisita de Trabajo del Alcalde de Aledo, Juan J. Andreo. Secretaria General UM. Julio 201
Reunión con el alcalde de Mula
No full textReunión de trabajo con el alcalde de Mula, Juan J. Moreno en el rectorado
Directed evolution of a Swiss knife ligninase: The unspecific peroxygenase
No full textAmong the broad repertory of the ligninolytic enzyme consortium, the unspecific peroxygenase (UPO) is shining as a versatile biocatalyst with potential applications in organic chemistry. UPO is a heme-thiolate peroxidase with mono(per)oxygenase activity for the selective oxyfunctionalization of C-H bonds. Fueled by catalytic concentrations of H2O2, which acts as both oxygen donor and as final electron acceptor, this stable, soluble and extracellular enzyme performs dozens of transformations that are of considerable interest in organic synthesis. In this talk I will describe the main attributes of this versatile enzyme, while reflecting on the directed evolution campaigns recently followed in our laboratory that set out to enhance the functional expression of UPO in yeast and improve the activity, as well as approximating its properties to the required industrial standards.
References:
Mate, D.M., Palomino, M.A., Molina-Espeja, P., Martin-Diaz, J. and Alcalde, M. (2017). Modification of the peroxygenative:peroxidative activity ratio in the unspecific peroxygenase from Agrocybe aegerita by structure-guided evolution. Protein Engineering Design and Selection. In press.
Molina-Espeja, P., Gomez de Santos, P., and Alcalde, M. (2017). Directed evolution of unspecific peroxygenase. In: Directed Enzyme Evolution: Advances and Applications. Alcalde, M. (Ed). Springer. 127-143.
Molina-Espeja, P., Cañellas, M., Plou, F.J., Hofrichter, M., Lucas, F., Guallar, V. and Alcalde, M. (2016). Synthesis of 1-naphthol by a natural peroxygenase engineered by directed evolution. ChemBioChem 17: 341-349.
Molina-Espeja, P., Ma, S., Mate, D.M., Ludwig, R. and Alcalde, M. (2015). Tandem-yeast expression system for engineering and producing unspecific peroxygenase. Enzyme Microbial Technology 73-74:29-33
Alcalde, M. (2015). Engineering the ligninolytic enzyme consortium. Trends in Biotechnology 33: 155-162.
Molina-Espeja, P., Garcia-Ruiz, E., Gonzalez-Perez, D., Ullrich, R., Hofrichter, M. and Alcalde, M. (2014). Directed evolution of unspecific peroxygenase from Agrocybe aegerita. Applied & Environmental Microbiology 80: 3496-350
Título: El mejor alcalde, el rey
No full textContén: I. El remedio en la desdicha ; El mejor alcalde, el re
Murder on the mountain: author talk with Peter J. Wosh
No full textAuthor talk by Peter J. Wosh on May 5th, 2022, on his book, "Murder on the Mountain: crime, passion, and punishment in gilded age New Jersey.
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