3,994,027 research outputs found
The dataset for "Observation of small Fermi pocket protected in a clean CuO2 sheet of high-Tc superconductor" by So Kunisada et al.
No full textThe dataset for "Observation of small Fermi pocket protected in a clean CuO2 sheet of high-Tc superconductor" by So Kunisada et al
SO MEE KWON
No full text학위논문(박사)--아주대학교 일반대학원 :의학계열,2014. 2TABLE OF CONTENTS
ABSTRACT i
TABLE OF CONTENTS iii
LIST OF FIGURES v
LIST OF TABLES vi
ABBREVIATIONS vii
I. INTORDUCTION 1
A. Background of study 1
B. Cancer genomic study of HCC 3
C. Aims of study 7
II. MATERIALS AND METHODS 10
A. MATERIALS 10
1. Cell-lines 10
2. Human tissues and FFPE samples 10
3. Preparation of RNA and genomic DNA 10
B. METHODS 11
1. Comparative genomic hybridization (CGH) profiling 11
2. Data pre-processing 12
3. DNA copy number profiling based on the T-statistic map (TM) 13
4. cDNA Microarray profiling 14
5. Determination of DNA copy number-dependent transcriptional deregulation 14
6. Validation of the prognostic relevance in the independent data set 15
7. Estimation of genomic DNA copy number by quantitative PCR (qPCR) 16
8. Gene set enrichment analysis 17
9. Short hairpin RNA (shRNA)-mediated knock-down experiment 17
10. Estimation of mRNA expression level using quantitative PCR (qPCR) 20
11. Cell viability and proliferation assay 21
12. Cell invasion assay 22
13. Western Blot analysis 22
14. Statistical analysis 23
III. RESULTS 25
A. Identification of subtype-specific DNA copy number alteration 25
B. Region of Interest at 6p showed subtype-specific DNA copy number alteration and concomitant transcriptional deregulation 32
C. IER3 is a putative biomarker for the ROI at 6p amplicon 33
IV. DISCUSSION 70
V. CONCLUSION 78
VI. REFERENCES 79
국문 요약 91DoctoralIn recent years, cancer heterogeneity, which is essentially inherent in various types of cancer, has been of interest to the cancer genome research. Many studies using various approaches have tried to solve the conundrum of so-called cancer heterogeneity. However, even though many successes have been earned in this area using the genomic analysis, the identification of precise cancer subtypes, which can be informative and useful from the biological and clinical point of view, still remains a challenge. Among the many trials, the multi-layered genomic profiles analysis, in which the genomic copy numbers and gene expression profiles are analyzed by the integrative way to define the chromosomal regions with both genomic copy number variation and concomitant transcriptional deregulation, is posited to provide a promising strategy to identify driver targets. Here, the integrative analysis of the DNA copy numbers and gene expression profiles of hepatocellular carcinoma (HCC) was performed. By comparing DNA copy numbers in HCC subtypes, which have been previously defined based on gene expression pattern, it was found that the HCC subtype showing aggressive phenotype without expressing stemness-related genes had DNA copy number alteration with concordant gene expression changes in the specific chromosomal area at 6p21-24. Among the genes residing at 6p21-24, IER3 was identified as a potential driver. The clinical utility of IER3 copy numbers was demonstrated by validating its clinical correlation in the independent cohorts. In addition, short hairpin RNA-mediated knock-down experiment revealed the functional relevance of IER3 in liver cancer progression. In conclusion, the results of this study suggest that genomic copy number alterations with transcriptional deregulation at 6p21-24 identify an aggressive HCC phenotype and a novel functional biomarker
Not So Quiet . . . A Novel
No full textThis story offers a rare, funny, bitter, feminist look at war from women actively engaged in it. Published in London in 1930, Not So Quiet...(on the Western Front) is a novel in autobiographical guise that describes a group of British women ambulance drivers on the French front lines during World War 1. As Voluntary Aid Detachment workers, the women pay for the privilege of driving the wounded through shell fire in the freezing cold, on no sleep and an inedible diet, under the watchful eye of their punishing commandant, nicknamed Mrs. Bitch.Front Cover -- Title Page -- Copyright Page -- Table of Contents -- Epigraph -- Chapter 1 -- Chapter 2 -- Chapter 3 -- Chapter 4 -- Chapter 5 -- Chapter 6 -- Chapter 7 -- Chapter 8 -- Chapter 9 -- Chapter 10 -- Chapter 11 -- Chapter 12 -- Afterword -- Notes -- About the Author -- About the Feminist Press -- Also Available from the Feminist PressThis story offers a rare, funny, bitter, feminist look at war from women actively engaged in it. Published in London in 1930, Not So Quiet...(on the Western Front) is a novel in autobiographical guise that describes a group of British women ambulance drivers on the French front lines during World War 1. As Voluntary Aid Detachment workers, the women pay for the privilege of driving the wounded through shell fire in the freezing cold, on no sleep and an inedible diet, under the watchful eye of their punishing commandant, nicknamed Mrs. Bitch.Description based on publisher supplied metadata and other sources.Electronic reproduction. Ann Arbor, Michigan : ProQuest Ebook Central, YYYY. Available via World Wide Web. Access may be limited to ProQuest Ebook Central affiliated libraries
Mapping the Discipline of the Olympic Games An Author-Cocitation Analysis
No full textThe authors conducted an author cocitation analysis on prominent authors writing about the Olympics during the 1990s. Author cocitation is an established bibliometric technique that can be used to measure the relative similarities of topics written about by the cited authors. This enables a visual representation of the “intellectual space” of the discipline, in this case the Olympics, to be created for the period under review. So core and peripheral research areas are identified, along with their major contributors. The representation appears as a two-dimensional cluster-enhanced map. Subject expertise was then applied to the results to place labels on the generated clusters of authors and their topics
Serum uric acid and gout: from the past to molecular biology.
No full textAbstract Objectives: This review will briefly present the epidemiology and risk factors of gout, with a focus on recent advances. Methods: Key papers for inclusion were identified by a PubMed search, and articles were selected according to their relevance for the topic, according to authors' judgment. Results and conclusions: Gout therapy has remained very much unchanged for the last 50 years, but recently we have seen the approval of another gout treatment: the xanthine oxidase inhibitor febuxostat, and several new drugs are now in the late stages of clinical testing. Together with our enhanced level of understanding of the pathophysiology of the inflammatory process involved, we are entering a new era for the treatment of gout
PCR-based analysis of the TCR repertoire in human autoimmune diseases [see comments]
No full textCharacterization of T cells at sites of autoimmune damage has been difficult. Now, however, polymerase chain reaction (PCR)-based methods are being used to analyse T-cell receptor (TCR) gene expression in such lesions. Here, Christopher Marguerie, Claudio Lunardi and Alex So summarize and interpret the most recent findings and describe the current understanding of TCR usage in autoimmune diseases
Dynamic programming with priority models for production planning with a central warehouse
No full textThis paper studies a production planning problem. A large soft-drink company in Hong Kong aims at finding a production planning model so as to minimize its inventory build-up and to maximize the demand satisfaction. We formulate the problem by using dynamic programming techniques. We further propose a heuristics model called Priority Model to reduce the computational complexity of this dynamic programming approach. Computational results based on real data sets are given to illustrate the effectiveness of our method
Seabed foraging by Antarctic krill: Implications for stock assessment, bentho-pelagic coupling, and the vertical transfer of iron
No full textA compilation of more than 30 studies shows that adult Antarctic krill (Euphausia superba) may frequent benthic habitats year-round, in shelf as well as oceanic waters and throughout their circumpolar range. Net and acoustic data from the Scotia Sea show that in summer 2-20% of the population reside at depths between 200 and 2000 m, and that large aggregations can form above the seabed. Local differences in the vertical distribution of krill indicate that reduced feeding success in surface waters, either due to predator encounter or food shortage, might initiate such deep migrations and results in benthic feeding. Fatty acid and microscopic analyses of stomach content confirm two different foraging habitats for Antarctic krill: the upper ocean, where fresh phytoplankton is the main food source, and deeper water or the seabed, where detritus and copepods are consumed. Krill caught in upper waters retain signals of benthic feeding, suggesting frequent and dynamic exchange between surface and seabed. Krill contained up to 260 nmol iron per stomach when returning from seabed feeding. About 5% of this iron is labile, i.e., potentially available to phytoplankton. Due to their large biomass, frequent benthic feeding, and acidic digestion of particulate iron, krill might facilitate an input of new iron to Southern Ocean surface waters. Deep migrations and foraging at the seabed are significant parts of krill ecology, and the vertical fluxes involved in this behavior are important for the coupling of benthic and pelagic food webs and their elemental repositories
Root and crown tot of olive caused dy Phytophthora spp.
No full textPhytophthora root and crown rot has been traditionally considered a minor disease of olive. However, in recent years it has
been recognized as an emerging problem in several olive-growing countries such as Australia, Italy and Spain probably as a
consequence of the expansion of plantings in new areas with heavy soils and the more intensive use of irrigation in both olive nurseries and commercial groves. The disease has been reported from most olive-growing countries and is caused by several soil -borne species of Phytophthora, including P. cinnamomi, P. citricola, P. cryptogea,
P. drechsleri, P. gonapodyides, P. inundata, P. megasperma,P. nicotianae and P.palmivora. Diagnosis is currently based on the
isolation and identification of isolates by both traditional and molecular methods. New molecular techniques are currently available that could be applied for both the identification of isolates and Phytophthora infections directly in host-tissues as well as insoil and water samples. A number of dedicated databases could improve the efficiency of these techniques. Moreover, DNA analysis has greatly contributed to phylogenetic studies of Phytophthora. Control of Phytophthora root and crown rot of olive is mainly based on preventive measures
Author Co-Citation Analysis (ACA): a powerful tool for representing implicit knowledge of scholar knowledge workers
No full textIn the last decade, knowledge has emerged as one of the most important and valuable organizational assets. Gradually this importance caused to emergence of new discipline entitled ―knowledge management‖. However one of the major challenges of knowledge management is conversion implicit or tacit knowledge to explicit knowledge. Thus Making knowledge visible so that it can be better accessed, discussed, valued or generally managed is a long-standing objective in knowledge management. Accordingly in this paper author co- citation analysis (ACA) will be proposed as an efficient technique of knowledge visualization in academia (Scholar knowledge workers)
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