14,390 research outputs found
Pinguicula (Lentibulariaceae) in central Italy: taxonomic study
No full textA morphometric and taxonomic study of Pinguicula in central Italy was carried out. Six allopatric units occur in this area, all belonging to sect. Pinguicula: P. fiorii Tammaro & Pace, P. vulgaris L. subsp. vulgaris and four new taxa: P. vulgaris subsp. anzalonei Peruzzi & F. Conti subsp. nova, P. vulgaris subsp. ernica Peruzzi & F. Conti subsp. nova, P. vulgaris subsp. vestina F. Conti & Peruzzi subsp. nova and P. vallis-regiae F. Conti & Peruzzi sp. nova. A key to the Italian species and subspecies of Pinguicula is provided
The geological map of Sardinia (Italy) at 1:250,000 scale
No full textOver the last 25 years the Italian national geological mapping program of the Italian Geological Survey (CARG Project, italian: Progetto Carta Geologica) at 1:50,000 scale has led to significant improvements in the geological knowledge for the Island of Sardinia (Italy). As a result, about one half of the island now is covered by new geological maps with 1:10,000–1:25,000 accuracy and geological maps at the 1:50,000 scale whose explanatory notes are available electronically. At the beginning of the CARG Project a geological map for Sardinia Island at 1:200,000 scale was published [Carmignani, L. (1996). Carta Geologica della Sardegna (1:200.000). Servizio Geologico Nazionale, Regione Autonoma della Sardegna], summarizing all the geological information available at that time, and a book with explanatory notes for the map was later published [Carmignani, L., Oggiano, G., Barca, S., Conti, P., Salvadori, I., Eltrudis, ... Pasci, S. (2001). Geologia della Sardegna: Note Illustrative della Carta Geologica della Sardegna in scala 1:200.000, Memorie Descrittive della Carta Geologica d'Italia (Vol. 60). Roma: Servizio Geologico d'Italia, 283 pp]. The enclosed Geological map of Sardinia at 1:250,000 scale incorporates all maps of the CARG Project, unpublished author studies and recently published maps and represents the most updated synthesis of an area characterised by a complex geological evolution that, with few exceptions, can be considered continuous during the last 540 Ma. The main events that influenced the geology of the island are the Variscan orogen that deeply involved the passive margin of North Gondwana and then the complex episodes that occurred in the present-day Mediterranean area after the accretion of Pangea up to the opening of the Tyrrhenian basin
Design, synthesis and biological characterization of PfGAPDH inhibitors
No full textMalaria has a tremendous health, social and economic impact on people living in tropical and subtropical regions of the world. WHO estimated 214 million cases and 438 000 deaths in 2015 and 3.2 billion people are globally at risk of infection1. Available treatments are becoming progressively less effective, mainly because parasites are constantly acquiring resistance toward the drug in use. Therefore, there is an urgent need of developing therapeutic agents acting on new targets.
Plasmodium parasites (P. falciparum is the most lethal), in their amastigote phase, produce energy only through glycolysis. In this pathway, GAPDH catalyses the first energy productive step and thus represent a valuable target for drug discovery. Known GAPDH inhibitors that bind to the active site are small nonselective molecules (iodoacetamide, 3-bromopyruvate), which irreversibly react with cysteine residues of the enzyme, including the catalytic one. We designed and synthesized the first series of inhibitors characterized by a 3-bromoisoxazoline warhead2, inspired by 3-bromoacivicin, a known inhibitor of reactive cysteine containing enzymes3. The compounds were assayed for the inhibitory activity on the P. falciparum isolated enzyme showing a biphasic irreversible inactivation. MS/MS studies of the digested protein demonstrated the mild reactivity of the exploited warhead, which only reacts with catalytic Cys (activated by a His residues). The human ortholog of GAPDH is only partially inhibited (up to 25%). We explained this through a negative cooperativity that prevents the alkylation of all the four monomers of hGAPDH, resulting in selectivity among the two isoforms of GAPDH4. Compounds were also assayed on cultures of the bloodstream form of P. falciparum and human cell lines in order to determine their in vitro antiparasitic activity and toxicity.
The goal of this project is to obtain compounds with improved potency and pharmacokinetic properties, suitable for in vivo studies on animal model of P. falciparum infections.
1. http://www.who.int/mediacentre/factsheets/fs094/en/
2. Bruno, S.; Pinto, A.; Paredi, G.; Tamborini, L.; De Micheli, C.; La Petra, V.; Marinelli, L.; Novellino, E.; Conti, P.; Mozzarelli, A. J. Med. Chem. 2014, 57(17), 7465-7471.
3. a) Conti, P.; Pinto, A.; Wong, P.E.; Major, L.L.; Tamborini, L.; Iannuzzi M.C.; De Micheli, C.; Barrett, M.P.; Smith, T.K. ChemMedChem 2011, 6(2), 329-333. b) Tamborini, L.; Pinto, A.; Smith, T.K.; Major, L.L.; Iannuzzi, M.C.; Cosconati, S.; Marinelli, L.; Novellino, E.; Lo Presti, L.; Wong, P.E.; Barrett, M.P.; De Micheli, C.; Conti, P. ChemMedChem 2012, 7(9), 1623-1634.
4. Bruno, S.; Margiotta, M.; Pinto, A.; Cullia, G.; Conti, P.; De Micheli, C.; Mozzarelli, A. Bioorg. Med. Chem. 2016, 24(12), 2654-2659
The radical right in Europe, between slogans and voting behavior. IHS Political Science Series No. 123, July 2011
No full textThe paper analyzes the radical right‘s attitudes toward the EU focusing in particular on the level of congruence between the programmatic statements of the central office and the voting behavior of their MEPs. It shows that although radical right parties represent a source of opposition to the EU, within the EP they express their dissent making use of the rules of the game, voting with the opposition more than the other forces do, but voting almost as much with the majority. The party public office in the EP is inserted in the legislative process and even more collusive with the other parties of both sides of the political spectrum than the Eurosceptical rhetoric and statements of central office makes the public believe
La vaccinazione anti COVID-19 in soggetti adolescenti: situazioni di contrasto nel processo decisionale
No full textL’estensione della vaccinazione anti COVID-19 agli adolescenti di età compresa tra 12 e 17 anni, al fine di salvaguardare la loro salute e contribuire a contenere l’espansione del virus, richiede forme di comunicazione adeguate all’età. I genitori devono essere coinvolti in ogni decisione relativa alla vaccinazione; d’altra parte è essenziale tener conto delle preferenze del soggetto adolescente e rispettare la sua autonomia.
Si intende prendere in considerazione gli aspetti etici e giuridici correlati al tema della vaccinazione anti COVID-19 in soggetti adolescenti, in particolare in relazione a situazioni di contrasto tra le parti nel processo decisionale
The geological map of Sardinia (Italy) at 1:250,000 scale
No full textOver the last 25 years the Italian national geological mapping program of the Italian Geological Survey (CARG Project, italian: Progetto Carta Geologica) at 1:50,000 scale has led to significant improvements in the geological knowledge for the Island of Sardinia (Italy). As a result, about one half of the island now is covered by new geological maps with 1:10,000–1:25,000 accuracy and geological maps at the 1:50,000 scale whose explanatory notes are available electronically. At the beginning of the CARG Project a geological map for Sardinia Island at 1:200,000 scale was published [Carmignani, L. (1996). Carta Geologica della Sardegna (1:200.000). Servizio Geologico Nazionale, Regione Autonoma della Sardegna], summarizing all the geological information available at that time, and a book with explanatory notes for the map was later published [Carmignani, L., Oggiano, G., Barca, S., Conti, P., Salvadori, I., Eltrudis, ... Pasci, S. (2001). Geologia della Sardegna: Note Illustrative della Carta Geologica della Sardegna in scala 1:200.000, Memorie Descrittive della Carta Geologica d'Italia (Vol. 60). Roma: Servizio Geologico d'Italia, 283 pp]. The enclosed Geological map of Sardinia at 1:250,000 scale incorporates all maps of the CARG Project, unpublished author studies and recently published maps and represents the most updated synthesis of an area characterised by a complex geological evolution that, with few exceptions, can be considered continuous during the last 540 Ma. The main events that influenced the geology of the island are the Variscan orogen that deeply involved the passive margin of North Gondwana and then the complex episodes that occurred in the present-day Mediterranean area after the accretion of Pangea up to the opening of the Tyrrhenian basin
The impact of COVID-19 pandemic on patients with chronic plaque psoriasis being treated with biologic therapy: the Northern Italy experience
No full textThe “severe acute respiratory syndrome coronavirus 2” (SARS-CoV-2) has spread over the four continents, causing the respiratory manifestations of Coronavirus disease-19 (COVID-19) and satisfying the epidemiological criteria for a pandemic [1]. As of April 1, 2020, more than one million COVID-19 positive cases have been identified and more than 54,000 deaths have occurred worldwide [2]. In Italy, 110,574 positive cases, 49,285 hospitalized patients and 13,155 deaths out of a population of 60,359,546 inhabitants, have been reported, respectively [3]. The highest number of deaths occurred in the northern Italian regions, i.e. Lombardy, Emilia-Romagna, Veneto and Piedmont [3]
Activity of a synthetic microbicidal peptide against Influenza A virus multiplication
No full text10
Activity of a synthetic microbicidal peptide against Influenza A virus multiplication
Conti G., Magliani V., Portincasa P., Conti S., Polonelli L.
Department of Pathology and Laboratory Medicine- Microbiology Section - University of Parma
A killer peptide (KP) engineered from the sequence of the variable region of a recombinant antiidiotypic antibody
representing the internal image of a killer toxin, is characterized by the wide spectrum of microbicidal activity against
pathogenic eukaryotic and prokaryotic microrganisms.
Based on the surprising sequence homology of KP with the light chain variable region of an antibody (HC63) that
prevents the haemagglutinin, HA, low pH fusogenic transition of Influenza A virus, we report the “in vitro” inhibition
of Influenza A virus multiplication in LLC-MK2 cells which is mediated by a marked reduction of glycosylation of HA,
as well as its synthesis.
The restriction of the production of mature virion particles could be the result of a defect involving the migration and /
or the association - insertion of virus haemagglutinin HA into the plasma membrane of infected LLC-MK2 cells treated
with KP.
Moreover, this hypothesis is also in good agreement with the haemadsorption as well as immunoprecipitation
experiment results
Design and synthesis of novel acidic amino acids acting at glutamate receptors and transporters
No full text- …
