1,720,959 research outputs found
Phylogeny of uncultured bacteria possessing pmoA gene and rRNA approach
Get PDF本研究では、アンプリコンPCRベースで16S rRNA遺伝子と機能遺伝子をリンクづけて解析する技術の開発を行い、環境サンプルに存在する未培養メタン酸化細菌の系統を明らかにすることを目的とした。純粋菌株を用いた実験では、エマルジョンを用いた遺伝子結合PCRを実施し、その精度を評価した。環境サンプルの調査では、土壌サンプルにおいて未培養メタン酸化細菌の割合が多いことがわかった。遺伝子結合PCRをそのサンプルへ適用し、1種類の未培養メタン酸化細菌の系統（16S rRNA遺伝子）を明らかにした。We developed an emulsion fusion PCR to link between 16S rRNA gene and functional gene. The fusion PCR was evaluated using a pure cultures experiment. And an uncultured methanotroph is discovered in forest soils as high population. We applied the developed tool to the sample and determined the phylogeny of the uncultured methanotroph.研究課題/領域番号:18K14777, 研究期間(年度):2018-04-01 – 2021-03-31出典：「メタン酸化酵素遺伝子を有する未培養微生物の網羅的系統分類とrRNAアプローチ」研究成果報告書 課題番号18K14777
（KAKEN：科学研究費助成事業データベース（国立情報学研究所））
（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-18K14777/18K14777seika/）を加工して作成金沢大学理工研究域地球社会基盤学系research repor
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
Development of microbial detection tool for link between phylogeny and function
Get PDF本研究では、未培養微生物群の16S rRNA遺伝子と機能遺伝子を簡便かつハイスループッドに両者をリンクづけて解析する技術を創成することを目的とした。まず、純粋細菌のゲノムDNAを用いて、申請者の研究アイディアの実証を行なった。その結果、微生物ゲノムから２種類の遺伝子を結合することが可能であった。次に、純粋細菌の細胞を用いて実施したところ、ゲノムを用いた時と実験条件は異なるものの、2種類の遺伝子結合が可能であった。シークエンス解析の結果からも、2種類の遺伝子配列が結合していることが確認できた。We demonstrated the idea for link between 16S rRNA gene and functional gene. The two genes were combined from the microbial genome/cell by fusion PCR. However we found that PCR condition is little bit different with the microbial genome and the cell. Sequencing result showed that two genes were combined correctly.研究課題/領域番号:16K14799, 研究期間(年度):2016-04-01 - 2018-03-31出典：研究課題「未培養微生物群の生理学的機能と系統分類を網羅的に結ぶ新規技術の創成」課題番号16K14799
（KAKEN：科学研究費助成事業データベース（国立情報学研究所））
（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-16K14799/16K14799seika/）を加工して作成金沢大学理工研究域地球社会基盤学系research repor
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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