1,734,768 research outputs found
Ghrelin attenuates the growth of HO-8910 ovarian cancer cells through the ERK pathway
No full textOvarian cancer is one of the most common causes of death from gynecologic tumors and is an important public health issue. Ghrelin is a recently discovered bioactive peptide that acts as a natural endogenous ligand of the growth hormone secretagogue receptor (GHSR). Several studies have identified the protective effects of ghrelin on the mammalian reproductive system. However, little research has been done on the effects of ghrelin on ovarian cancer cells, and the underlying mechanisms of these effects. We sought to understand the potential involvement of mitogen-activated protein kinases (MAPKs) in ghrelin-mediated inhibition of growth of the ovarian line HO-8910. We applied different concentrations of ghrelin and an inhibitor of the ghrelin receptor (D-Lys3-GHRP-6) to HO-8910 cells and observed the growth rate of cells and changes in phosphorylation of the MAPKs ERK1/2, JNK and p38. We discovered that ghrelin-induced apoptosis of HO-8910 cells was though phosphorylated ERK1/2, and that this phosphorylation (as well as p90rsk phosphorylation) was mediated by the GHSR. The ERK1/2 pathway is known to play an essential part in the ghrelin-mediated apoptosis of HO-8910 cells. Hence, our study suggests that ghrelin inhibits the growth of HO-8910 cells primarily through the GHSR/ERK pathway
Ghrelin enhances cisplatin sensitivity in HO-8910 PM human ovarian cancer cells
No full textAbstract Background Resistance to platinum-based chemotherapy is one of the crucial problems in ovarian cancer treatment. Ghrelin, a widely distributed peptide hormone, participates in a series of cancer progression. The aim of this study is to determine whether ghrelin influences the sensitivity of ovarian cancer to cisplatin, and to demonstrate the underlying mechanism. Methods The anti-tumor effects of ghrelin and cisplatin were evaluated with human ovarian cancer cells HO-8910 PM in vitro or in vivo. Cell apoptosis and cell cycle were analyzed via flow cytometry assay. The signaling pathway and the expression of cell cycle protein were analyzed with Western Blot. Results Our results showed that treatment with ghrelin specifically inhibited cell proliferation of HO-8910 PM and sensitized these cells to cisplatin via S phase cell cycle arrest, and enhanced the inhibitory effect of cisplatin on tumor growth of HO-8910 PM derived xenografts in vivo. Treatment with ghrelin inhibited the expression of p-Erk1/2 and p-p38, which was opposite the effect of cisplatin. However, under the treatment of ghrelin, cisplatin treatment exhibited a stronger effect on inhibiting P21 expression, upregulating p-CDK1 and cyclin B1 expression, and blocking cell cycle progression. Mechanistically, ghrelin promoted S phase cell cycle arrest and upregulated p-CDK1 and cyclin B1 expression induced by cisplatin via inhibition of p38. Conclusion This study revealed a specifically inhibitory effect of ghrelin on platinum-resistance via suppressing p-P38 and subsequently promoting p-CDK1 mediated cell cycle arrest in HO-8910 PM
Linked collectors and determiners for: On centipedes (Chilopoda) of Albania, 2.
No full textNatural history specimen data linked to collectors and determiners held within, "On centipedes (Chilopoda) of Albania, 2". Claims or attributions were made on Bionomia by volunteer Scribes, <a href="http://bionomia.net/dataset/2421e270-cdee-494f-8910-d05c178223fb">https://bionomia.net/dataset/2421e270-cdee-494f-8910-d05c178223fb</a> using specimen data from the dataset aggregated by the Global Biodiversity Information Facility, <a href="https://gbif.org/dataset/2421e270-cdee-494f-8910-d05c178223fb">https://gbif.org/dataset/2421e270-cdee-494f-8910-d05c178223fb</a>. Formatted as a Frictionless Data package
Linked collectors and determiners for: On centipedes (Chilopoda) of Albania, 2.
No full textNatural history specimen data linked to collectors and determiners held within, "On centipedes (Chilopoda) of Albania, 2". Claims or attributions were made on Bionomia by volunteer Scribes, <a href="http://bionomia.net/dataset/2421e270-cdee-494f-8910-d05c178223fb">https://bionomia.net/dataset/2421e270-cdee-494f-8910-d05c178223fb</a> using specimen data from the dataset aggregated by the Global Biodiversity Information Facility, <a href="https://gbif.org/dataset/2421e270-cdee-494f-8910-d05c178223fb">https://gbif.org/dataset/2421e270-cdee-494f-8910-d05c178223fb</a>. Formatted as a Frictionless Data package
Linked collectors and determiners for: The ' red-tailed' Lasioglossum (Dialictus) (Hymenoptera: Halictidae) of the western Nearctic.
No full textNatural history specimen data linked to collectors and determiners held within, "The ' red-tailed' Lasioglossum (Dialictus) (Hymenoptera: Halictidae) of the western Nearctic". Claims or attributions were made on Bionomia by volunteer Scribes, <a href="http://bionomia.net/dataset/9e062836-3946-4ac6-8910-304cadff0d4b">https://bionomia.net/dataset/9e062836-3946-4ac6-8910-304cadff0d4b</a> using specimen data from the dataset aggregated by the Global Biodiversity Information Facility, <a href="https://gbif.org/dataset/9e062836-3946-4ac6-8910-304cadff0d4b">https://gbif.org/dataset/9e062836-3946-4ac6-8910-304cadff0d4b</a>. Formatted as a Frictionless Data package
Linked collectors and determiners for: Actualización de la Colección de Collembola de México y ácaros edáficos. Catálogo de oribátidos de México.
No full textNatural history specimen data linked to collectors and determiners held within, "Actualización de la Colección de Collembola de México y ácaros edáficos. Catálogo de oribátidos de México". Claims or attributions were made on Bionomia by volunteer Scribes, <a href="http://bionomia.net/dataset/d8f60448-42c4-489c-8910-d8f0b58b24b1">https://bionomia.net/dataset/d8f60448-42c4-489c-8910-d8f0b58b24b1</a> using specimen data from the dataset aggregated by the Global Biodiversity Information Facility, <a href="https://gbif.org/dataset/d8f60448-42c4-489c-8910-d8f0b58b24b1">https://gbif.org/dataset/d8f60448-42c4-489c-8910-d8f0b58b24b1</a>. Formatted as a Frictionless Data package
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
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