1,720,997 research outputs found
免疫担当細胞の遺伝子発現プロファイル解析による癌免疫の解明
Get PDFC57BL/6マウス由来の大腸癌細胞株MC38を同系マウスへ経脾経門脈的に接種し成立させた肝内播種巣には浸潤リンパ球がほとんど認められなかった。そのため、よりヒト悪性腫瘍に類似した免疫応答を伴う癌モデルの開発を試みた。C57Lマウス由来の肝細胞癌株Hepa1-6を経脾経門脈的に2x10^6個のHepa1-6細胞を投与したC57B1/6マウスの一部に、56日後にHepa1-6腫瘍の肝への著明な播種を認め播種癌部へのリンパ球浸潤が認められた。このHepa1-6肝内播種巣を回収し、in vitroにて、2種類の細胞株Hepa1-6clone Aおよびclone Bを樹立した。Hepa1-6 clone Aは、ケモカインCCL20、CCL2、またinterleukin 13 receptor,alpha、IL-4 receptor,alpha、IL-12 receptor,beta、CDld、CD81の発現低下が認められ、宿主の免疫防御機構に対する抵抗性が推測された。Hepa 1-6 clone Aを脾臓へ5x10^6個接種させたC57BL/6マウス(♀、8週齢)を接種後23日後に開腹、肝内に播種された腫瘍には組織学的にリンパ球の浸潤が認められた。本研究において、免疫担当細胞の遺伝子発現プロファイル解析による癌に対する免疫応答を解析するより有用なモデルが確立された。同マウスの末梢血液をRNA安定化剤へ収集しRNAを抽出し、DNAマイクロアレイを用いて担癌状態におけるマウスの末梢血液細胞の遺伝子発現を解析中である。研究課題/領域番号:18790452, 研究期間(年度):2006 – 2007出典：「免疫担当細胞の遺伝子発現プロファイル解析による癌免疫の解明」研究成果報告書 課題番号18790452
（KAKEN：科学研究費助成事業データベース（国立情報学研究所））
（https://kaken.nii.ac.jp/ja/grant/KAKENHI-PROJECT-18790452/)を加工して作成金沢大学附属病院research repor
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Analysis of characteristics of cellular subfractions in adipose tissue-derived stromal cells for development of liver repair/regenerative therapy
Get PDF本研究では、マウス非培養脂肪組織由来間質細胞群の細胞分画を解析し、その特性を検討した。また、マウス肝疾患モデルを用いて、肝疾患状態に対する効果を検討した。非培養脂肪組織由来間質細胞群には、約10～20%のCD45陽性細胞が存在した。CD45陽性細胞は、表面抗原発現解析、遺伝子発現より、M2フェノタイプマクロファージ様の特性を有することを明らかとした。急性肝炎マウスに対する非培養脂肪由来間質細胞群、間質細胞群のCD45陽性細胞分画の投与による治療効果を確認した。非培養脂肪組織由来間質細胞群のCD45陽性細胞分画の炎症修飾能における重要性が示唆された。In this study, we analyzed the cellular fractions of murine uncultured adipose tissue derived stromal cells and assessed their characteristics. In addition, we investigated their effect on the liver disease condition using murine liver disease models. We found that the 10-20% of uncultured adipose tissue-derived stromal cells expressed CD45 antigen. CD45+ cells were disclosed to be characteristics of M2 phenotype macrophage in view of surface antigen analysis as well as gene expression analysis. Administration of the entire ulcutred adipose tissue derived stromal cells and their CD45+ fraction were effective to acute murine hepatitis. Thus, these results indicated that the relative importance of CD45+ cellular fraction of the uncultured adipose tissue derived stromal cells in the context of their immunomodulatory effect.研究課題/領域番号:26460995, 研究期間(年度):2014-04-01 - 2017-03-31出典：「脂肪組織由来間質細胞群の細胞分画特性解析と肝修復再生療法の開発」研究成果報告書 課題番号26460995
（KAKEN：科学研究費助成事業データベース（国立情報学研究所））
（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-26460995/26460995seika/）を加工して作成金沢大学医薬保健研究域医学系research repor
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Comprehesive gene expression analysis of cancer-infiltrating lymphocytes and peripheral blood cells for elucidation of cancer immunity kinesis
Get PDF増殖から消褪までの特徴を示すマウス皮下肝癌モデルを用いて、癌局所の浸潤炎症細胞と末梢血液細胞の遺伝子発現プロファイルの解析を行った。末梢血液細胞の遺伝子発現プロファイルは、経時的グループ別に類似性を示した。腫瘍径がピーク期の際の腫瘍内炎症細胞と末梢血液細胞の遺伝子発現の共通した特徴には、IL-6シグナル、変性蛋白に対する応答が示された。また、各々の特徴的なプロセスには、腫瘍内炎症細胞では、インターフェロンシグナル、NK細胞傷害性、補体系が、末梢血液細胞ではIL-10応答、アンフォテリン応答がそれぞれ示された。包括的遺伝子発現解析によって、癌における局所と全身の炎症の共通および各々に特徴的な応答を解明できる可能性が示された。Using murine subcutaneous hapatoma model which shows the proliferating and succeeding extinguishing phases, analysis of gene expression profile for local cancer-infiltrating inflammatory cells as well as peripheral blood cells was performed. The features of gene expression profiles for peripheral blood were distinct depending on the groups divided by the days after cancer cells injection. The common feature of gene expression of local cancer-infiltrating inflammatory cells as well as peripheral blood cells for mice with the tumors at the maximum size levels showed IL-6 signal and response to unfoleded proteins. As for the individually characteristic processes of gene expression, interferon signaling, NK cell cytotoxicity, complement system for local cancer-infiltrating inflammatory cells, and IL-10 anti-inflammatory response, amphoterin signaling for peripheral blood, were featured, respectively. These suggest that comprehensive analysis of gene expression of local cancer inflammatory response and peripheral blood cells will reveal the common as well as the distinguished features of local and systemic inflammatory responses.研究課題/領域番号:20590766, 研究期間(年度):2008-2010出典：研究課題「癌浸潤リンパ球と末梢血液細胞の包括的遺伝子発現解析による癌免疫動態の解明」課題番号20590766
（KAKEN：科学研究費助成事業データベース（国立情報学研究所））
（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-20590766/20590766seika/）を加工して作成金沢大学医薬保健研究域医学系research repor
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
Gene transfer programming of mesenchymal stem cells for repairing and regeneration of the liver, and its applocation for liver regeneration therapy
Get PDF脂肪組織由来間質細胞への遺伝子導入による肝細胞への分化誘導を試みる研究を行った。GFPタグ付HNF4a、HNF1b発現プラスミド(pCMV6-HNF4a-GFP, pCMV-6HNF1b-GFP)をエレクトロポレーション法にて導入した。導入後15日目の細胞を回収し、リアルタイムPCRにて遺伝子発現解析を行った。pCMV6-HNF4a-GFP導入細胞ではHNF4aの発現を認めたが、Albumin発現は見られなかった。pCMV6-HNF1b-GFP導入細胞は、HNF1bの発現、Albuminの発現双方認められなかった。脂肪組織由来間質細胞への遺伝子導入による肝細胞分化には、改良が必要であった。The experimental trial of differentiation of adipose-tissue derived stromal cells to hepatocyte by means of gene transduction was conducted. The pCMV6-HNF4a-GFP expressing GFP-tagged HNF4a and the pCMV6-HNF1b-GFP expressing GFP-tagged HNF1b was transduced to adipose tissue-derived stromal cells by electroporation method. Cells at day 15 after transduction was assessed for gene expression by real-time detective PCR. Cells transduced with pCMV6-HNF4a-GFP expressed HNF4a, however, did not express albumin. Cells transduced with pCMV6-HNF1b-GFP neither expressed HNF1b nor albumin. Improvement is required for differentiation of adipose tissue derived stromal cells by electroporation method.研究課題/領域番号:23590968, 研究期間(年度):2011-2013出典：研究課題「遺伝子導入による間葉系幹細胞の肝修復再生能プログラミングと肝再生療法への応用」課題番号23590968
（KAKEN：科学研究費助成事業データベース（国立情報学研究所））
（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-23590968/23590968seika/）を加工して作成research repor
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