1,753,707 research outputs found
FRET in a Polymeric Nanocarrier: IR-780 and IR-780-PDMS
No full textWe
introduce a method to monitor the integrity of micellar nanocarriers
using a novel fluorescent dye, IR-780-PDMS and Förster resonance
energy transfer (FRET) as a readout. In addition, these dye-loaded
nanocarriers can be used as a phototoxic agent in vitro. Mainly, a
nanocarrier was designed, based on a previously described amphiphilic
ABA-copolymer (Pip-PMOXA-PDMS-PMOXA-Pip) scaffold that incorporates
the fluorescent FRET dye partners IR-780-PDMS (donor) and IR-780 (acceptor).
The confirmation of FRET (that only occurs when donor and acceptor
are in the required close proximity of less than ∼10 nm) in
the nanocarriers is used to prove that the acceptor dye (IR-780) is
still contained in its hydrophobic core. We measured such FRET signals
of the nanocarriers also upon cellular uptake into HeLa cells using
fluorescence-lifetime imaging microscopy (FLIM). Confocal laser scanning
microscopy after incubation with nanocarriers demonstrated the intracellular
uptake of the particles and their localization in an intracellular
granular pattern. To demonstrate the intactness of the nanocarriers
by detection of FRET we measured the fluorescence lifetime (FLIM)
of the donor dye. FLIM showed that both types of lifetimes, that of
the quenched donor, and that of the unquenched donor were present,
in a granular pattern and homogeneously in the cytosol, respectively,
indicating the presence of intracellular intact and disintegrated
micellar nanocarriers. These data show that the herewith-described
FRET method allows monitoring the intactness of nanocarriers while
en route to the target, and also that the cargo is delivered and released
within a potential target cell. In addition, near-infrared (NIR) irradiation
of IR-780-loaded micellar nanocarriers leads to photocytotoxicity,
which we demonstrated in in vitro experiments. Our findings open potential
avenues in photodynamic therapy (PDT) of cancer
AIEgens Conjugation Improves the Photothermal Efficacy and Near-Infrared Imaging of Heptamethine Cyanine IR-780
No full textNear-infrared
(NIR) fluorescent probes can deeply penetrate through tissues with
little damage. To facilitate image-guided theranostics, researchers
usually apply a desired amount of photosensitizers to achieve effective
photothermal responses. However, these probes could easily suffer
from low photostability and aggregated-caused quenching effect in
high concentrations. In this paper, the rational incorporation of
an aggregated-induced emission (AIE) unit into the structure of heptamethine
cyanine IR-780 is reported. Using tetraphenylethene (TPE) as an AIE
core, we synthesize three TPE-modified IR-780 probes (IR-780 AIEgens)
via different linkages. The IR-780 derivatives all show enhanced AIE
features, in which the probe with an ether linkage (IR780-O-TPE) is
superior in rapid cell uptake, high targeting capacity, and good photostability.
Moreover, IR780-O-TPE exhibits the strongest cytotoxicity to HeLa
cells (IC50 = 3.3 μM). The three IR-780 derivatives
displayed a photothermal response in a concentration-dependent manner,
in which IR-780 AIEgens are more cytotoxic than IR-780, with IC50 of 0.3 μM under 808 nm laser irradiation. In tumor-bearing
mice, the optimal probe IR780-O-TPE also showed a more effective photothermal
response than IR-780. By illustrating the relationship between aggregation
state with photophysical properties, cell imaging, and cytotoxicity,
this work is helpful in modulating NIR-based photosensitizers into
AIE features for efficient image-guided theranostics
Claas Lexion 780 Terratrac, el relevo generacional.
Get PDFEl día 25 de junio llevamos a cabo en la Puebla de Montalbán (Toledo) un ensayo de verificación de las prestaciones de una cosechadora Claas Lexion 780 Terratrac, comparándolas con las características de una máquina más antigua, una Claas Lexion 530 Montana que disponía de un cabezal McDon, empleándose además en el primer caso un sistema de auto-guiado. La profusión de datos disponibles no deja lugar a la especulación, todo, o mejor dicho, casi todo puede ser cuantificado
Resolución UNRN N° 780/2009. Adjudica la compra objeto de la contratación directa N° 29/2009.
No full textFil: Universidad Nacional de Río Negro (U). Universidad Nacional de Río Negro. Río Negro, ArgentinaResolución UNRN N° 780/2009. Adjudica la compra objeto de la contratación directa N° 29/2009.fals
The Town Mouse and the Country Mouse (spiral #780)
No full textIn terms of printing, this version is identical with the hardbound #780. See my comments there. The pages of this book have a different, honeycombed kind of paper. The first page is separate from the binding, and the others are loose after fifty years of wear. The second-last page is torn, and someone has pencilled over a few pages and the country mouse's eye on the very last page. I now count eight versions that I have found of the same simple book
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
ALVIN 780 Dive 780 6
No full textALVIN 780 Dive 780 6The runtime for this video is 18m 10s.Alvin dive 780 took place on August 26, 1977 during voyage LU-94. The Chief Scientist was W. Ryan in the operating area of Continental Slope. The purposewas described as 'GEOLOGY' and the sponsor as 'J.A.', or Joint Agreement of National Science Foundation, Office of Naval Research, and National Oceanic and Atmospheric Administration.The items in this collection are embargoed in the system in keeping with copyright requirements. However, they are available to users upon request. Please contact the MBLWHOI Library to access the items at [email protected] of this material was supported by a Recordings at Risk grant from the Council on Library and Information Resources (CLIR). The grant program is made possible by funding from The Andrew W. Mellon Foundation. CLIR is an independent, nonprofit organization that forges strategies to enhance research, teaching, and learning environments in collaboration with libraries, cultural institutions, and communities of higher learning. To learn more, visit www.clir.org and follow CLIR on Facebook and Twitter.2200-01-0
ALVIN 780 Dive 780 1
No full textALVIN 780 Dive 780 1The runtime for this video is 30m 05s.Alvin dive 780 took place on August 26, 1977 during voyage LU-94. The Chief Scientist was W. Ryan in the operating area of Continental Slope. The purposewas described as 'GEOLOGY' and the sponsor as 'J.A.', or Joint Agreement of National Science Foundation, Office of Naval Research, and National Oceanic and Atmospheric Administration.The items in this collection are embargoed in the system in keeping with copyright requirements. However, they are available to users upon request. Please contact the MBLWHOI Library to access the items at [email protected] of this material was supported by a Recordings at Risk grant from the Council on Library and Information Resources (CLIR). The grant program is made possible by funding from The Andrew W. Mellon Foundation. CLIR is an independent, nonprofit organization that forges strategies to enhance research, teaching, and learning environments in collaboration with libraries, cultural institutions, and communities of higher learning. To learn more, visit www.clir.org and follow CLIR on Facebook and Twitter.2200-01-0
ALVIN 780 Dive 780 3
No full textALVIN 780 Dive 780 3The runtime for this video is 32m 46s.Alvin dive 780 took place on August 26, 1977 during voyage LU-94. The Chief Scientist was W. Ryan in the operating area of Continental Slope. The purposewas described as 'GEOLOGY' and the sponsor as 'J.A.', or Joint Agreement of National Science Foundation, Office of Naval Research, and National Oceanic and Atmospheric Administration.The items in this collection are embargoed in the system in keeping with copyright requirements. However, they are available to users upon request. Please contact the MBLWHOI Library to access the items at [email protected] of this material was supported by a Recordings at Risk grant from the Council on Library and Information Resources (CLIR). The grant program is made possible by funding from The Andrew W. Mellon Foundation. CLIR is an independent, nonprofit organization that forges strategies to enhance research, teaching, and learning environments in collaboration with libraries, cultural institutions, and communities of higher learning. To learn more, visit www.clir.org and follow CLIR on Facebook and Twitter.2200-01-0
- …
