1,725,141 research outputs found
Chrysin inhibits sphere formation in SMMC-7721 cells via modulation of SHP-1/STAT3 signaling pathway
No full textYanqin Zhang,1,2 Feng Chen,1 Xinghua Xiao,1 Weinan Pan,2 Qing Yuan,3 Jianguo Cao31Department of Pharmacy, Xiangya Hospital of Central South University, Changsha, 410008, People’s Republic of China; 2Department of Pharmacy, Hunan Food and Drug Vocational College, Changsha, 410208, People’s Republic of China; 3Department of Pharmaceutical Science, Medical College, Hunan Normal University, Changsha 410013, People’s Republic of ChinaBackground: Chrysin is a natural flavonoid which has been identified as a candidate anti-cancer agent due to its inhibitory effect on a variety of cancer cells, including targeted inhibition of sphere formation in hepatocellular carcinoma (HCC) cell lines. However, the mechanism by which chrysin modulates HCC spheres remains unclear.Materials and methods: In this study, we investigate the effect of chrysin on the regulation of SHP-1 and its downstream signal molecule STAT3 to explain the mechanism by which chrysin inhibits sphere formation of HCC cell lines.Results: Here, we found that SHP-1 protein expression was markedly down-regulated in the spheres from both SMMC-7721 and MHCC97H cells. Chrysin significantly inhibited sphere formation and upregulated the expression of SHP-1 protein in both SMMC-7721 and MHCC97H cells, as well as reduced p-STAT3 and Twist1 expressions in SMMC-7721 cells. Furthermore, knockdown of SHP-1 in SMMC-7721 cells resulted in the induction of p-STAT3 and Twist1 protein expression and antagonizing the inhibitory effect of chrysin on sphere formation in SMMC-7721 cells.Conclusion: Overall, the study findings demonstrated that chrysin acts as a candidate for the treatment of HCC through modulating SHP-1/STAT3 signaling pathway.Keywords: chrysin, hepatocellular carcinoma, SHP-1, STAT3, sphere formatio
U12-induced apoptosis in SMMC-7721.
No full text<p>Morphological and quantitative changes in SMMC-7721 cells after being (<b>A</b>) left untreated, (<b>B</b>) treated with 100 µM U12 for 24 h, or (<b>C</b>) pretreated with 50 µM Z-VAD-fmk or (<b>D</b>) 20 µM Z-IETD-fmk for 1 h. (<b>E</b>) Western blotting was used to estimate PARP cleavage from the 100 µM U12 for 24 h treatments. (<b>F</b>) Detection of apoptotic SMMC-7721 cells in the presence of 80 µM U12 for 2 h and 7 h using Annexin V-FITC/PI analysis. (<b>G</b>&<b>H</b>) Activation of caspase-3, -8, and -9 was evaluated using a caspase activity kit after indicated concentration of U12 treatment at 2 h and 7 h, respectively. (<b>I</b>) Western blot analysis of PARP cleavage on SMMC-7721 cells untreated and treated with indicated concentration of U12 at 12 h.</p
Arenaria longipedunculata. Gillespie, Saarela, Consaul & Bull 7721 (CAN 592340)
No full text<p>Arenaria longipedunculata. Gillespie, Saarela, Consaul & Bull 7721 (CAN 592340)</p
Apoptosis Induced by Aqueous Extracts of Crocodile Bile in Human Heptacarcinoma SMMC-7721
No full textNatural Science Foundation of China [81101502]; GuangZhou TuoLong Biological Technology Co., Ltd. [XDHT2012106A]In the present study, effects of aqueous extracts from Crocodylus siamensis bile (AE-CB) on SMMC-7721 cell growth, cell cycle, and apoptosis were investigated by 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide assay, inverted microscopy, fluorescence microscopy, propidium iodide (PI) single-and fluorescein isothiocyanate (FITC)/PI double-staining flow cytometry, and western blotting. Our data have revealed that AE-CB significantly inhibited the growth of SMMC-7721 cell and arrested cell cycle at G0/G1 phase. SMMC-7721 cells showed typical apoptotic morphological changes after treated with AE-CB for 48 h. Cell death assay indicated that SMMC-7721 cells underwent apoptosis in a dose-dependent manner induced by AE-CB. In addition, AE-CB treatment could down-regulate the protein level of Bcl-2 and upregulate the Bax, leading to the increase in the ratio of Bax to Bcl-2 in SMMC-7721 cells. Meanwhile, it was observed that the expression of Survivin and c-Myc decreased, but the expression of P53 increased. All these events were associated with increase of reactive oxygen species. The data indicated that mitochondrial pathway might play an important role in bile extract-induced apoptosis in SMMC-7721 cells. These results provide significant insight into the anticarcinogenic action of bile extract on SMMC-7721 cells
Inhibitory effect of emodin on human hepatoma cell line SMMC-7721 and its mechanism
Get PDFBackground: Da Huang (Radix et Rhizoma Rhei) is the dried root or rhizome of Rheum palmatum L., Rheum tanguticum Maxim ex Balf. or Rheum officinale Braill of family Polygonaceae. It has heat clearing, damp drying, fire purging and toxin removing effects. Because of its definite curative efficacy, it has been widely applied in clinical settings.Objective: To study the inhibitory effect of emodin on human hepatoma cell line SMMC-7721 and its mechanism.Methods: MTT assay, flow cytometry and electron microscopy were used to investigate the inhibitory effect of different concentrations of emodin on human hepatoma cell line SMMC-7721.Results: 12 h, 24 h and 48 h after the action of 20, 40 and 80 umol/L emodin on SMMC-7721 cells, the proliferation of human hepatoma SMMC-7721 cells was inhibited; the inhibitory effects showed time-and concentration-dependence. 48 h after the action of different concentrations of emodin on SMMC-7721 cells, cells in G2/M phase increased significantly, while the proportion of S phase cells gradually declined.Conclusion: Emodin can inhibit human hepatoma cell line SMMC-7721.Keywords: emodin; SMMC-7721; MTT assa
Ethylacetate extract from Tetrastigma hemsleyanum inhibits proliferation and induces apoptosis in HepG2 and SMMC-7721 cells
No full textShipin Chen,1,* Meixiu Luo,1,* Liang Ma,2 Wenjun Lin1 1College of Forestry, Fujian Agriculture and Forestry University, Fuzhou City, Fujian Province 350001, China; 2Institute of Art of Landscape, Fujian Agriculture and Forestry University, Fuzhou City, Fujian Province 350001, China *These authors contributed equally to this work Purpose: This study aimed to investigate the effect of ethylacetate extract from Tetrastigma hemsleyanum (EET) on the proliferation and apoptosis of HepG2 and SMMC-7721 cells and determine the underlying mechanisms. Materials and methods: HepG2 and SMMC-7721 cells were cultured in vitro until the exponential growth phase and then treated with different concentrations of EET for 24 h. We performed a colony forming assay to determine colony forming ability, CCK8 assay to detect cell proliferation, Annexin V–FITC/PI double staining to analyze cell apoptosis, and Western blot to measure the protein expression of Caspase-3, Bcl-2, and Bax. Results: EET significantly inhibited the proliferation of HepG2 and SMMC-7721 cells in a concentration- and time-dependent manner (P<0.05). After treatment with 0, 50, 100, 150, 200, and 250 μg/mL EET for 24 h, HepG2 the proliferation rates were 100.00%±0.00%, 90.33%±1.76%, 67.67%±0.88%, 47.33%±0.88%, 37.00%±0.00%, and 30.33%±0.67%, respectively, and 100.00%±0.00%, 18.25%±1.05%, 19.99%±0.59%, 23.42%±0.46%, 29.70%±0.79%, and 29.8%±0.41% for SMMC-7721 cells, respectively. After treatment with 0, 50, 100, 150, 200, and 250 μg/mL EET for 24 h, the apoptotic rates were 11.08%±0.72%, 27.44%±0.51%, 32.92%±0.41%, 26.20%±0.47%, 22.92%±0.24%, and 55.60%±0.08%, for HepG2 cells, respectively, and 59.18%±0.17%, 41.24%±2.05%, 52.54%±0.39%, 50.54%±1.08%, and 57.44%±1.93% for SMMC-7721 cells, respectively. Compared with the treatment groups, the control group showed a significantly lower apoptotic rate (47.91%±1.09%, P<0.05). EET at the different concentrations downregulated the protein expression of Caspase-3 in HepG2 cells and upregulated it in SMMC-7721 cells; it also downregulated the protein expression of Bcl-2 in HepG2 and SMMC-7721 cells and upregulated the protein expression of Bax in HepG2 and SMMC-7721 cells. Conclusion: These findings suggest that EET exerts antiproliferative and proapoptotic effects against HepG2 and SMMC-7721 cells mediated by downregulation or upregulation of Caspase-3 expression. Our study may help to develop EET for the pharmacological treatment of hepatoblastoma or hepatocellular carcinoma. Keywords: Tetrastigma hemsleyanum Diels et Gilg, EET, cell proliferation, apoptosis, HepG2 cells, SMMC-7721 cells, Caspase-3, Bcl-
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Transfection of C/EBPα-saRNA in SMMC-7721 and HepG2 cells resulted in inhibition of migration.
No full text(A–B) Gene expression of C/EBPα (A) and albumin (B) in SMMC-7721 and HepG2 cells transfected with C/EBPα-saRNA or scramble-saRNA as the control for 48 h. (C) Transwell assays show reduced migration of SMMC-7721 and HepG2 cells transfected with C/EBPα-saRNA compared with the control after 18 h. Magnification, 100×. Data represent mean±SD.</p
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