1,744,822 research outputs found
Circ_0001955 promotes the progression of non-small cell lung cancer via miR-769-5p/EGFR axis
No full textTo elaborate on the role of circular RNA 0001955 (circ_0001955) on the proliferation and apoptosis of non-small cell lung cancer (NSCLC) cells and its underlying mechanism. Circ_0001955 expression in NSCLC was screened out through bioinformatics analysis based on GEO database. Circ_0001955, microRNA-769-5p (miR-769-5p), and epidermal growth factor receptor (EGFR) expression in NSCLC tissues and cell lines was examined using quantitative real-time PCR (qRT-PCR) and Western blot. Cell proliferation and apoptosis were examined using the CCK-8 method, BrdU experiment and flow cytometry analysis, respectively. Bioinformatics prediction, dual-luciferase reporter gene experiment and RNA immunoprecipitation (RIP) experiments were applied to validate the targeting relationship between miR-769-5p and circ_0001955 and the 3’ UTR of EGFR. Pearson’s correlation analysis was employed to validate the correlations among them. Circ_0001955 expression was up-regulated in NSCLC tissues and cell lines, and its overexpression was strongly associated with increased tumor TNM stage and lymph node metastasis. Circ_0001955 overexpression enhanced the proliferation and restrained the apoptosis in NSCLC cells, whereas knocking down circ_0001955 exerted the opposite effects. Circ_0001955 directly targeted miR-769-5p and negatively regulated its expression. EGFR, a target gene of miR-769-5p, could be indirectly and positively regulated by circ_0001955. Correlation analysis indicated that circ_0001955 was negatively correlated with miR-769-5p expression, while circ_0001955 was positively correlated with EGFR expression. Circ_0001955 facilitates the proliferation and represses the apoptosis of NSCLC cells by modulating miR-769-5p/EGFR axis.</p
Late Pleistocene stable isotope record of planktonic foraminifera from ODP Site 124-769
No full textA reconstruction of late Pleistocene surface water carbon isotopic (delta13C) variability is presented from Ocean Drilling Program (ODP) site 769 in the Sulu Sea in the western tropical Pacific. The Sulu Sea is a shallowly silled back arc basin with a maximum sill depth of 420 m. Site 769 was drilled on a bathymetric high in 3643 m of water and has average late Pleistocene sedimentation rates of 8.5 cm/kyr. The oxygen isotope record (delta18O) of Globigerinoides ruber at site 769 shows a strong correlation with the SPECMAP stacked delta18O record, attesting to the continuity of sediment archive at the site. Surface delta13C displays consistent glacial-interglacial variability which averages ~0.9 per mil and has varied from 0.75 to 1.1 per mil over the last 800 kyr. Comparison to surface water delta13C records in the South China Sea and western tropical Pacific suggests that the glacial-interglacial surface delta13C variability is regional in scale. Planktonic delta13C data from ODP site 677 in the eastern Pacific is also coherent with the site 769. Additionally, we have found that the site 769 surface delta13C record is coherent at periods of 100 and 41 kyr with deepwater delta13C records from the Pacific. The highest correlation occurs with the deep eastern Pacific, where benthic delta13C data from cores RC13-110 and ODP site 677 closely match the Sulu Sea surface water record. We evaluate several possible controls of surface water delta13C in the Sulu Sea that may explain the coherent timing with Pacific deepwater delta13C records. These include variations in terrestrial organic matter flux to the basin, the upwelling of subsurface water and productivity changes, and the influx of western Pacific intermediate water to the Sulu Sea. Our preferred explanation involves a region of upper intermediate water upwelling in the far western Pacific which has been shown to outgas CO2 from subsurface waters into surface waters. Upwelling also occurs in the area of Panama Basin site 677. These equatorial upwelling zones could potentially provide a route by which Pacific intermediate water can modulate the delta13C composition of certain Pacific surface water locations. Future reconstructions of late Pleistocene surface water delta13C variability in the western Pacific and Indonesian seas will be required to further evaluate the source of the glacial-interglacial surface water delta13C change
MÉTODOS IMUNO-HISTOQUÍMICOS APLICADOS AO DIAGNÓSTICO E PESQUISA DE DOENÇAS EM ANIMAIS
No full textO Laboratório de Patologia Veterinária da Unipampa (LPV-Unipampa) presta importante serviço de auxílio diagnóstico que envolve a realização de avaliações macroscópicas e análise histopatológica através de métodos histoquímicos tradicionais como hematoxilina-eosina. A disponibilidade da imuno-histoquímica (IHQ), técnica que visa a detecção e visualização de antígenos (celulares ou de agentes infecciosos) em tecidos é uma forma complementar de diagnóstico e pesquisa que possibilita aumentar a acurácia e especificidade diagnóstica. Várias doenças animais na região de abrangência do LPV-Unipampa se beneficiam da utilização desta técnica, tanto do ponto de vista de diagnóstico, como na realização de pesquisas. Neste contexto verifica-se uma aplicabilidade imediata da técnica no diagnóstico e pesquisas envolvendo Leishmaniose canina, uma doença infecciosa de caráter zoonótico e amplamente difundida na população canina da região. Da mesma forma o uso da IHQ na determinação exata da origem de células tumorais em diferentes neoplasmas faz da mesma uma técnica fundamental. O objetivo do presente trabalho é descrever as etapas envolvidada na implantação da IHQ no LPV-Unipampa, bem como a aplicação da mesma em estudos específicos, como na leishmaniose canina e no diagnóstico oncológico
Additional file 1 of Exosomal transfer of miR-769-5p promotes osteosarcoma proliferation and metastasis by targeting DUSP16
No full textAdditional file 1: Table S1. Expression of miR-769-5p and DUSP16 according to patients’ clinical features
Resolución UNRN N° 769/2009. Modificar la designación como profesora adjunta
No full textFil: Universidad Nacional de Río Negro (U). Universidad Nacional de Río Negro. Río Negro, ArgentinaResolución UNRN N° 769/2009. Modificar la designación como profesora adjuntafals
Ms. 769(2)
No full textManuscrito número 769(2) de la Biblioteca Histórica de la USAL84 h. [=85], [2] en blanco : papel ; 198 x 145 mmOmitido en la foliación un folio entre h. 62 y 63Manuscrito griego, copiado por Hernán Núñez probablemente en Alcalá, hacia 1514 (cf. Signes). Línea tirada con anotaciones marginales en griego y alguna en latínFol. 1-82: Escolios;
Contiene además, en fol. 83-84v: Olímpicas XIII y XIV [Griego
Des enjeux e&769;thiques lie&769;s a&768; l&8217;utilisation des donne&769;es de recherches en collaboration internationale
No full textCette étude de cas en santé mondiale met au jour les enjeux éthiques associés à l'utilisation des données de recherche collectées dans le cadre d'une collaboration internationale ayant recours à des assistants de recherche
Des enjeux e&769;thiques lie&769;s a&768; l&8217;utilisation des donne&769;es de recherches en collaboration internationale
No full textCette étude de cas en santé mondiale met au jour les enjeux éthiques associés à l'utilisation des données de recherche collectées dans le cadre d'une collaboration internationale ayant recours à des assistants de recherche
Managing benzimidazole resistance in the potato dry rot fungus
No full textBulletin no. 769 Moscow, Idaho :University of Idaho, College of Agriculture, Cooperative Extension System, 1994-10-01. Author(s): Nolte, Philli
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