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Study of the probiotic Lactobacillus casei B 7280 action for different durations of use on the resistance of the bee organism
No full textThe scientific support of modern beekeeping is aimed at obtaining safe ecofriendly products, developing tools and methods for stimulating reproduction and increasing the bees’ resistance to various pathogens, as well as protection from adverse environmental conditions. There is a trend to use new effective means of natural origin, whose mechanisms of action differ from synthetic substances and preparations due to the activation of the body’s protective reactions at the physiological level. Probiotic Lactobacillus casei IMV B-7280 has an effective stimulating effect on physiological processes under various environmental and experimental conditions of bees’ life. The physiological effect of this probiotic is associated with the intestinal bacterial microflora normalization and taking part in the body’s protective reactions modulation. Therefore, the purpose of the research was to determine the effect of the probiotic preparation L. casei B-7280 on the content of protein, peroxidation products, and catalase activity in homogenates of bee body tissues for different durations of use. Bees of the control (C) group were fed with 60% sugar syrup in the amount of 1 ml/group/day. Experimental groups additionally received a probiotic L. casei B-7280 solution at a concentration of 106 CFU/ml: experimental group 1 (Е1) — daily; experimental group 2 (Е2) — once every two days; experimental group 3 (Е3) — once every four days; experimental group 4 (Е4) — once a week. The total duration of drinking syrup and probiotics was 4 weeks. An increase in the content of total protein in the tissues of the entire bees’ body was established in Е1–Е3 groups, but these differences are not significant, which may indicate the absence of an appreciable effect of the probiotic L. casei B-7280 on the protein concentration in bee tissues. An increase in the catalase activity of bee body tissues was observed by 79.80% and 38.07% in Е1, Е2 groups (P<0.05) compared to the control, and for bees of Е1 group by 52.75% (P<0.05) compared to the preparatory period. The content of LHP decreased by 10.00%; 9.23% and 10.38% (P<0.05) in Е1, Е2 and Е3 groups, respectively, compared to the control. The content of TBC-active products tended to decrease in experimental groups Е1, Е2, Е3, and in Е4 by 14.98% (P<0.01) compared to the preparatory period. The research results showed that the addition of L. casei B-7280 affected the antioxidant activity in the bees’ body at different times of its feeding, which was accompanied by a decrease in the content of TBA-active products (MDA) and lipid hydroperoxides in the homogenates of the bees’ body tissues in all experimental groups, except Е3 group, and affected the viability of bees
ОБҐРУНТУВАННЯ СПОСОБУ ВВЕДЕННЯ СУБСТАНЦІЇ LACTOBACILLUS CASEI ІМВ В-7280 У ДОСЛІДНІ ЗРАЗКИ ПЕСАРІЇВ ІЗ ПРОБІОТИЧНОЮ АКТИВНІСТЮ
Get PDFThe aim of the work. To justify the rational administration way of the substance Lactobacillus casei ІМВ В-7280 with probiotic activity into the pessaries experimental samples composition used for the prevention and treatment of dysbiotic disorders in women.
Materials and Methods. Object of the study: substance Lactobacillus casei IMB-7280 (synthesized by the D. K. Zabolotny Institute of Microbiology and Virology). According to the State Pharmacopoeia of Ukraine methods, the substance crystallographic characteristics and its dissolution in the following solvents: polysorbate-80, peach oil, 1,2-propylene glycol, macrogol -400, vaseline oil, glycerin, purified water were determined. Statistical analysis of the results was performed with MedStat v.5.2 statistic software package.
Results and Discussion. It has been determined that the investigated substance according to the crystallographic characteristics consists of crystals and their fragments of anisodiametric shape with a rough surface and broken edges, powder particles have the ability to agglomerate. The solubility study indicates the optimal solubility is in a mixture of purified water and polysorbate-80: reduction of agglomeration, linear powder particle size (to 0,1 µm) and uniform distribution in the medium.
Conclusions. During the development of drugs with probiotic activity to ensure high bioavailability, it is advisable to administer the substance Lactobacillus casei IMB-B-7280 into the dosage forms in the form of an aqueous solution (1:20) dissolving with addition of surfactant (polysorbate-80) with pre-grinding to 0.5–1.0 µm.Мета роботи. Обґрунтувати раціональний спосіб введення субстанції Lactobacillus casei ІМВ В-7280 із пробіотичною активністю до складу дослідних зразків песаріїв, що призначені для профілактики та лікування дисбіотичних порушень у жінок.
Матеріали і методи. Об’єкт дослідження: субстанція Lactobacillus casei ІМВ В-7280 (самостійно синтезована Інститутом мікробіології і вірусології імені Д. К. Заболотного). Відповідно до Державної Фармакопеї України (ДФУ) проводили визначення кристалографічних характеристик субстанції та її розчинення у таких розчинниках: полісорбат-80, олія персикова, 1,2-пропіленгліколь, макрогол-400, вазелінове масло, гліцерин, вода очищена. Статистичну обробку результатів здійснювали за допомогою ліцензійного програмного забезпечення MedStat v.5.2.
Результати й обговорення. Визначено, що досліджувана субстанція за кристалографічними характеристиками складається з кристалів та їх уламків анізодіаметричної форми з шорсткуватою поверхнею та ламаними краями, частинки порошку мають здатність до агломерації. Дослідження розчинності вказує на найоптимальніші показники розчинності у водному середовищі з додаванням полісорбату-80: зниження агломерації, лінійних розмірів частинок порошку (до 0,1 мкм) та рівномірний розподіл у даному середовищі.
Висновки. При розробці лікарських засобів (ЛЗ) із пробіотичною активністю для забезпечення високої біодоступності доцільне введення субстанції Lactobacillus casei ІМВ В-7280 до складу лікарських форм (ЛФ) у вигляді водного розчину (1:20) при розчиненні з додаванням поверхнево активної речовини (ПАР) (полісорбату-80) з попереднім подрібненням до 0,5–1,0 мкм
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Live Performance for ‘Goodbye to That!: Student Suite’ EDA 7280 Curriculum Theory
No full textPerformance photos, program, and data collection plan from the invited visit to EDA 7280 Curriculum Theory, University of South Florida Tampa, Instructor, Dr. Vonzell Agosto, Saturday March 11th, 2017 1:00 to 3:00 pm, Tampa FL
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Lipid composition and peroxidation products in the body tissues in bees under the action of different doses of nanotechnological Ge citrate and the probiotic Lactobacillus casei В-7280
No full textModern methods of preserving honey bees focus on increasing their viability, feeding level and productivity development during different growth periods. There is a tendency to use new effective remedies of natural origin. Their action mechanisms differ from synthetic substances and drugs due to the activation of the body’s protective reactions at the physiological level. The probiotic Lactobacillus casei IMV B-7280 has an effective stimulating effect on physiological processes in bees under various environmental and experimental conditions. The physiological effect of this probiotic is associated with normalizing of the intestinal bacterial microflora and participation in modulating body’s protective reactions. The resistance of honey bees also depends on mineral nutrition, which affects metabolic processes at the tissue, organ and system levels and determines the vitality and resistance of the organism. Mineral elements are involved in protein, lipid, carbohydrate and energy metabolism, they activate enzyme systems. Therefore, the aim of the research was to determine the effect of the probiotic L. сasei B-7280 in combination with different doses of Ge nanotechnological citrate (NTC) on the lipid composition and peroxidation products content in bees’ tissues. Bees of the control group were fed 60% sugar syrup (SS) in the amount of 1 cm3/group/day. The bees of the first experimental group (R1) were fed 1 cm3 of SS, 0.1 μg of Ge NTC and a solution of probiotic L. casei B-7280 (106 cells/cm3); the second experimental group (R2) received 0.2 μg of Ge citrate and probiotic L. casei B-7280 (106 cells/cm3). The feeding duration was 34 days. It was established that feeding sugar syrup, L. сasei and different doses of Ge citrate increased the content of phospholipids, mono- and diacylglycerols in the R2 group, cholesterol esterification in the R1 group, and decreased free cholesterol, non-esterified fatty acids compared to the K group and P period. The results indicate a dose-dependent effect of Ge NTC on lipid metabolism in body tissue homogenates. There was an increase in the triacylglycerols level in the lipids in all groups during the experimental period. The biological effect of nanotechnological Ge citrate and L. сasei cause a decrease of lipid peroxidation processes in bees of the experimental groups
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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