1,746,049 research outputs found
miR-708-5p inhibited STK4-mediated Hippo-YAP1 signaling pathway.
No full text(A) The targeting sequence of miR-708-5p in the 3’UTR of STK4 was predicated using Starbase (http://starbase.sysu.edu.cn/index.php). (B) The interaction between miR-708-5p and STK4 was identified using dual-luciferase reporter assay. **P P ##P < 0.01 compared with the siRNA-negative control (siNC). (D) The expression of STK4 and p-YAP1 in MDSCs overexpressed miR-708-5p was verified by immunofluorescence staining. Nuclear DAPI staining was presented in blue. Scale bar is 200 μm.</p
Overexpression of miR-708-5p promoted the differentiation of MDSCs into IPCs.
No full text(A) The expression of miR-708-5p in MDSCs and MDSCs-derived IPCs at stage 1–5 of differentiation was verified by qRT-PCR. **P P P P P P ###P < 0.001 compared with the MDSCs-derived IPCs. (E-F) Co-immunostaining of insulin/C-peptide, insulin/Pdx1, and insulin/Nkx6.1 in MDSCs-derived IPCs with miR-708-5p overexpression. The nuclear was stained with DAPI in blue. Scale bar is 200 μm.</p
Resolución UNRN N° 708/2009. Modificar la designación.
No full textFil: Universidad Nacional de Río Negro (U). Universidad Nacional de Río Negro. Río Negro, ArgentinaResolución UNRN N° 708/2009. Modificar la designación para desempeñarse como docentes del curso de ingreso 2010.fals
miR-708-5p promotes fibroblast–like synoviocytes' cell apoptosis and ameliorates rheumatoid arthritis by inhibition of Wnt3a/β-catenin pathway
No full textJie Wu,1 Wenqiang Fan,1 Ling Ma,1 Xiuqin Geng2 1Department of Rheumatology and Immunology, Central Hospital of Xinxiang, Henan 453000, People’s Republic of China; 2Department of Endocrinology, Central Hospital of Xinxiang, Henan 453000, People’s Republic of China Aim: MicroRNAs (miRNA) are a class of small, highly conserved noncoding RNA molecules, which contain 18–28 nucleotides and are involved in the regulation of gene expression. It has been proved that microRNAs play a very important role in several key cellular processes, such as cell differentiation, cell cycle progression, and apoptosis, as well as in autoimmune disease. One recently identified miRNA, miR-708-5p, has been demonstrated to have profound roles in suppressing oncogenesis in different types of tumors. However, the role of miR-708-5p in rheumatoid arthritis (RA) remains to be fully elucidated. Therefore, in this study, we are aiming to identify the role of miR-708-5p in RA. Methods: The expression level of miR-708-5p in synovial tissues of patients with RA is much lower than in non-RA controls. The effects of miR-708-5p on cell apoptosis, colony formation, and migration in fibroblast-like synoviocytes were assessed in MH7A cells. Results: Results showed that delivery of miR-708-5p mimics into synovial fibroblasts MH7A could induce cell apoptosis and inhibit colony formation and migration. In addition, miR-708-5p mimics significantly inhibit Wnt3a/β-catenin pathway activity both in transcription and protein level, which could be reversed by the addition of R-spondin 1, an activator of Wnt pathway. R-spondin 1 could also reverse the inhibition of cell survival and proliferation, which was induced by miR-708-5p mimics in MH7A. Moreover, injection of miR-708-5p mimics into collagen-induced rat RA model could ameliorate the RA index and decrease Wnt3a/β-catenin expression in rat joint tissues. Conclusion: Therefore, we concluded that miR-708-5p is likely to be involved in RA pathogenesis via inhibition of Wnt3a/β-catenin pathway. Keywords: miR-708-5p, rheumatoid arthritis, cell proliferation, Wn
Supplementary Methods, Supplementary Figures 1-5, Supplementary Tables 1-2 from Downregulation of DNMT3A by miR-708-5p Inhibits Lung Cancer Stem Cell–like Phenotypes through Repressing Wnt/β-catenin Signaling
No full textFigure S1. MiR-708-5p regulates stem cell-like properties in NSCLC cells; Figure S2. DNMT3A is a direct target of miR-708-5p; Figure S3. (A) Schematics of DNMT3A synonymous mutation at the binding sites of miR-708-5p in the wide type coding DNA sequence (CDS) of DNMT3B; Figure S4. qRT-PCR (A) and western blot (B) analysis the expression of β-catenin in miR-708-5p-transfected cells compared with the corresponding control ones in A549, Calu-3 and 95D cells; Figure S5. (A) Comparison of relative expression of miR-708-5p between two groups of adenocarcinomous patients in TCGA cohort, classified by molecular subtype based on EGFR mutation, ALK fusion, TP53 mutation and KRAS mutation, respectively; Table S1: The clinicopathological information for PQG samples; Table S2: The clinicopathological information for TCGA lung cancer dataset containing miRNA-seq data [1].</p
Verlustliste Nr. 708 (Nr. 708 / 1919)
No full textVERLUSTLISTE NR. 708
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Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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