1,724,252 research outputs found
Determining the IC50 of M4K1062 with ACVR1/ALK2-NanoLuc and different concentrations of Tracer-6908
No full textThe EC50 of Tracer-6908 with ACVR1-c-nanoLuc and ideal conditions for the target engagement assay have been determined in the previous post. However, it is still necessary to verify that the IC50 values determined in the assay are closed enough approximation to the actual IC50 values of the compounds. If the IC50 values are strongly influenced by the concentration of Tracer-6908 used, multiple assays with progressively lower Tracer-6908 will need to be performed. The actual IC50 of the compounds can then be estimated by plotting the Tracer-6908 concentrations against the corresponding IC50 values and regressing to no Tracer-6908. To address this, multiple nanoBRET target engagement assays were setup with increasing concentrations of Tracer-6908 with the same serial dilution of M4K1962 (starting from the EC50 of 65nM)
Logistic regression estimates of neonatal mortality (N = 6908).
No full textLogistic regression estimates of neonatal mortality (N = 6908).</p
The Curious Case of NGC 6908
Get PDFThe object NGC 6908 was once thought to simply be a surface brightness enhancement in the eastern spiral arm of the nearby spiral galaxy NGC 6907. Based on an examination of near-infrared imaging, the object is shown to in fact be a lenticular S0(6/7) galaxy hidden in the optical glare of the disk and spiral structure of the larger galaxy. New radial velocities of NGC 6908 [3060 ± 16 km s^(-1) (emission); 3113 ± 73 km s^(-1) (absorption)] have been obtained at the Baade 6.5 m and the du Pont 2.5 m telescopes at Las Campanas, Chile, placing NGC 6908 at the same expansion velocity distance as NGC 6907 (3190 ± 5 km s^(-1)), eliminating the possibility of a purely chance line-of-sight coincidence. The once-enigmatic asymmetries in the disk and outer spiral structure of NGC 6907 are now explained as being due to an advanced merger event. Newly discovered tails and debris in the outer reaches of this galaxy further support the merger scenario for this system. This pair of galaxies is a rather striking example of two objects discovered over 100 years ago, whose true nature was lost until modern detectors operating at infrared wavelengths gave us a new (high-contrast) look. Other examples of embedded merger remnants may also reveal themselves in the growing samples of near-infrared imaging of nearby galaxies, and a pilot study does reveal several other promising candidates for follow-up observations
[Boston and Maine, Sketch No. 6908]
No full textThis engine drawing card was created for Boston and Maine Railroad, Class 14-52 1/4 F. J-14 1/4 F. Sketch 6908. See also Est. Based on-2-10-2B. Copy Spec. AL Cus. Spec A-9612. No BLW Spec
[Boston and Maine, Wt. Est. for Sketch No. 6908]
No full textThis engine drawing card was created for Boston and Maine Railroad, Class 14-52 1/4 F. J-14 1/4 F. Sketch wt. est for sk. 6908. Copy Spec. [field left blank
HI aperture synthesis and optical observations of the pair of galaxies NGC 6907 and 6908
No full textNGC 6908, an S0 galaxy situated in the direction of NGC 6907, was only recently recognized as a distinct galaxy, instead of only a part of NGC 6907. We present 21-cm radio synthesis observations obtained with the Giant Metrewave Radio Telescope (GMRT) and optical images and spectroscopy obtained with the Gemini-North telescope of this pair of interacting galaxies. From the radio observations, we obtained the velocity field and the H I column density map of the whole region containing the NGC 6907/8 pair, and by means of the Gemini multi-object spectroscopy we obtained high-quality photometric images and 5 angstrom resolution spectra sampling the two galaxies. By comparing the rotation curve of NGC 6907 obtained from the two opposite sides around the main kinematic axis, we were able to distinguish the normal rotational velocity field from the velocity components produced by the interaction between the two galaxies. Taking into account the rotational velocity of NGC 6907 and the velocity derived from the absorption lines for NGC 6908, we verified that the relative velocity between these systems is lower than 60 km s(-1). The emission lines observed in the direction of NGC 6908, not typical of S0 galaxies, have the same velocity expected for the NGC 6907 rotation curve. Some emission lines are superimposed on a broader absorption profile, which suggests that they were not formed in NGC 6908. Finally, the H I profile exhibits details of the interaction, showing three components: one for NGC 6908, another for the excited gas in the NGC 6907 disc and a last one for the gas with higher relative velocities left behind NGC 6908 by dynamical friction, used to estimate the time when the interaction started in (3.4 +/- 0.6) x 10(7) yr ago
Determining the EC50 of Tracer-6908 with ACVR1/ALK2-NanoLuc
No full textPromega had provided us with Tracer-6908 that was developed collaboratively with SGC. Before the tracer can be used in nanoBRET assay for compound IC50, it is necessary to determine the optimal tracer concentration. The tracer concentration needs to be sufficient to produce adequate BRET signal while at the same time low enough to not obscure the competition by test compounds.
The blog post for this Zenodo page is as following:
https://opennotebook.thesgc.org/?p=1282&preview=tru
Determining the EC50 of Tracer-6908 with ACVR1/ALK2-NanoLuc
No full textPromega had provided us with Tracer-6908 that was developed collaboratively with SGC. Before the tracer can be used in nanoBRET assay for compound IC50, it is necessary to determine the optimal tracer concentration. The tracer concentration needs to be sufficient to produce adequate BRET signal while at the same time low enough to not obscure the competition by test compounds.
The blog post for this Zenodo page is as following:
https://opennotebook.thesgc.org/?p=1282&preview=tru
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
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