1,723,343 research outputs found
Chicago, Burlington & Quincy (CBQ) 6132
No full textA photograph print showing Chicago, Burlington & Quincy (CBQ) 6132, 2-10-2 (M-2-A), Denver, CO
Image1_Increased miR-6132 promotes deep vein thrombosis formation by downregulating FOXP3 expression.jpeg
No full textBackgroundDeep vein thrombosis (DVT) is associated with aberrant gene expression that is a common peripheral vascular disease. Here, we aimed to elucidate that the epigenetic modification of forkhead box protein 3 (FOXP3) at the post-transcriptional level, which might be the key trigger leading to the down-regulation of FOXP3 expression in DVT.MethodsIn order to explore the relationship between microRNAs (miRNAs) and FOXP3, mRNA and microRNA microarray analysis were performed. Dual luciferase reporter assay was used to verify the upstream miRNAs of FOXP3. Quantitative real-time polymerase chain reaction, flow cytometry and Western blot were used to detect the relative expression of miR-6132 and FOXP3. Additionally, DVT models were established to investigate the role of miR-6132 by Murine Doppler Ultrasound and Hematoxylin-Eosin staining.ResultsMicroarray and flow cytometry results showed that the FOXP3 expression was decreased while miR-6132 level was increased substantially in DVT, and there was significant negative correlation between miR-6132 and FOXP3. Moreover, we discovered that overexpressed miR-6132 reduced FOXP3 expression and aggravated DVT formation, while miR-6132 knockdown increased FOXP3 expression and alleviated DVT formation. Dual luciferase reporter assay validated the direct binding of miR-6132 to FOXP3.ConclusionCollectively, our data elucidate a new avenue through which up-regulated miR-6132 contributes to the formation and progression of DVT by inhibiting FOXP3 expression.</p
Project Title: Development and Field Evaluation of the Next Generation of HMA Mix Design Procedures (Project 0-6132)
No full text2012PDFTech Reporthttps://ntlrepository.blob.core.windows.net/lib/46000/46800/46854/0-6132-P1.zipProject 0-6132Mix designHot mix asphaltAsphalt pavementsPavement performancePerformance testsBindersDense graded aggregatesPavement crackingOverlays (Pavements)Pavements, Asphalt--CrackingPavements--Design and constructionPavements--OverlaysPavements--PerformanceBinders (Materials)TexasTexas A&M Transportation InstituteScullion, TomWalubita, LubindaZhou, FujieTexas Transportation InstituteNTL-HIGHWAY/ROAD TRANSPORTATION-DesignNTL-HIGHWAY/ROAD TRANSPORTATION-Pavement Management and PerformanceUS Transportation CollectionPart of Project: Development and Field Evaluation of the Next Generation of HMA Mix Design Procedures (Project 0-6132)Study 0-6132 has promoted the development and implementation of the balanced mix design (BMD) approach for selecting the optimal asphalt content for all of TxDOT\u2019s hot mix asphalts (HMA), including Item 341. In this approach the engineering properties are measured in both the laboratory design and the trial batch with both the Hamburg Wheel Track test (Tex Method 242F) and the Overlay test (Tex Method 248F).The supporting files zip contains this report as well as: TechMemo-Task 6 (Project 0-6132) Atlanta 01.pdf; TechMemo-Task 6 (Project 0-6132) Atlanta 02.pdf; TechMemo-Task 6 (Project 0-6132) Atlanta 03.pdf; TechMemo-Task 6 (Project 0-6132) Bryan.pdf; The Bryan CAM Mix-Design Lab Report 01_FINAL.pdf; TechMemo-Task 6 (Project 0-6132) LAREDO 01.pdf; TechMemo-Task 6 (Project 0-6132) LOREDO 02.pdf; LOREDO 02 Performance.pdf; Childress_Type D.pdf; Fort Worth_Type D.pdf; Odessa_Type C.pdf; San Antonio_Type C.pdf; San Antonia_Type D.pdf; APT-ALF Testing _SUMMARY REPORT.pdf; and, Draft to replace 341 from Study 6132.pdf.75
Image2_Increased miR-6132 promotes deep vein thrombosis formation by downregulating FOXP3 expression.jpeg
No full textBackgroundDeep vein thrombosis (DVT) is associated with aberrant gene expression that is a common peripheral vascular disease. Here, we aimed to elucidate that the epigenetic modification of forkhead box protein 3 (FOXP3) at the post-transcriptional level, which might be the key trigger leading to the down-regulation of FOXP3 expression in DVT.MethodsIn order to explore the relationship between microRNAs (miRNAs) and FOXP3, mRNA and microRNA microarray analysis were performed. Dual luciferase reporter assay was used to verify the upstream miRNAs of FOXP3. Quantitative real-time polymerase chain reaction, flow cytometry and Western blot were used to detect the relative expression of miR-6132 and FOXP3. Additionally, DVT models were established to investigate the role of miR-6132 by Murine Doppler Ultrasound and Hematoxylin-Eosin staining.ResultsMicroarray and flow cytometry results showed that the FOXP3 expression was decreased while miR-6132 level was increased substantially in DVT, and there was significant negative correlation between miR-6132 and FOXP3. Moreover, we discovered that overexpressed miR-6132 reduced FOXP3 expression and aggravated DVT formation, while miR-6132 knockdown increased FOXP3 expression and alleviated DVT formation. Dual luciferase reporter assay validated the direct binding of miR-6132 to FOXP3.ConclusionCollectively, our data elucidate a new avenue through which up-regulated miR-6132 contributes to the formation and progression of DVT by inhibiting FOXP3 expression.</p
Increased miR-6132 promotes deep vein thrombosis formation by downregulating FOXP3 expression
Get PDFBackgroundDeep vein thrombosis (DVT) is associated with aberrant gene expression that is a common peripheral vascular disease. Here, we aimed to elucidate that the epigenetic modification of forkhead box protein 3 (FOXP3) at the post-transcriptional level, which might be the key trigger leading to the down-regulation of FOXP3 expression in DVT.MethodsIn order to explore the relationship between microRNAs (miRNAs) and FOXP3, mRNA and microRNA microarray analysis were performed. Dual luciferase reporter assay was used to verify the upstream miRNAs of FOXP3. Quantitative real-time polymerase chain reaction, flow cytometry and Western blot were used to detect the relative expression of miR-6132 and FOXP3. Additionally, DVT models were established to investigate the role of miR-6132 by Murine Doppler Ultrasound and Hematoxylin-Eosin staining.ResultsMicroarray and flow cytometry results showed that the FOXP3 expression was decreased while miR-6132 level was increased substantially in DVT, and there was significant negative correlation between miR-6132 and FOXP3. Moreover, we discovered that overexpressed miR-6132 reduced FOXP3 expression and aggravated DVT formation, while miR-6132 knockdown increased FOXP3 expression and alleviated DVT formation. Dual luciferase reporter assay validated the direct binding of miR-6132 to FOXP3.ConclusionCollectively, our data elucidate a new avenue through which up-regulated miR-6132 contributes to the formation and progression of DVT by inhibiting FOXP3 expression
N.V. Smith & D. Wilson, Modern Linguistics
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6132.pdf (Publisher’s version ) (Open Access
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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