1,727,308 research outputs found
Data_Sheet_1_miR-4666-3p and miR-329 Synergistically Suppress the Stemness of Colorectal Cancer Cells via Targeting TGF-β/Smad Pathway.ZIP
No full textQuiescent caner stem cells are identified as a subpopulation of colon cancer cells in dormant state and possess strong stem-cell like characteristics. Previously, we have identified this subpopulation in colorectal cancer (quiescent colon cancer stem cells, QCCSCs), and find QCCSCs are sensitive to the apoptotic effect of IFN-γ, which is attributed to their high IFN-γR expression levels. Microarray and bioinformatic analysis indicate miR-4666-3p is low expressed in QCCSCs and target IFN-γR1/2, which is proved by luciferase assay and western-blot. Furthermore, we find miR-4666-3p could also target TGF-βR1 to block the activation of TGF-β1/Smad pathway, therefore function as a tumor suppressor gene to inhibit the stemness of colon cancer cells. Besides, compared with QCCSCs, we find the TGF-β1 expression also decreased with the weakening of stemness properties. In terms of mechanism, our result reveal TGF-β1 is the target gene of miR-329, which is also high expressed in non-QCCSCs. Thereafter, we perform gain- and loss- function experiments to confirm the synergistic effect between miR-4666-3p and miR-329 in blocking the activation of TGF-β/Smad pathway. Finally, we evaluate the expression of both miR-4666-3p and miR-329 in 73 tumor specimens and paired normal tissue, and find both two miRNAs are related to unfavorable prognosis and advanced tumor stage in colorectal cancer. Our study revealed a novel epigenetic regulation mechanism in colon cancer stem cells, which could be exploited as a novel therapeutic strategy for cancer treatment.</p
Table_1_miR-4666-3p and miR-329 Synergistically Suppress the Stemness of Colorectal Cancer Cells via Targeting TGF-β/Smad Pathway.DOCX
No full textQuiescent caner stem cells are identified as a subpopulation of colon cancer cells in dormant state and possess strong stem-cell like characteristics. Previously, we have identified this subpopulation in colorectal cancer (quiescent colon cancer stem cells, QCCSCs), and find QCCSCs are sensitive to the apoptotic effect of IFN-γ, which is attributed to their high IFN-γR expression levels. Microarray and bioinformatic analysis indicate miR-4666-3p is low expressed in QCCSCs and target IFN-γR1/2, which is proved by luciferase assay and western-blot. Furthermore, we find miR-4666-3p could also target TGF-βR1 to block the activation of TGF-β1/Smad pathway, therefore function as a tumor suppressor gene to inhibit the stemness of colon cancer cells. Besides, compared with QCCSCs, we find the TGF-β1 expression also decreased with the weakening of stemness properties. In terms of mechanism, our result reveal TGF-β1 is the target gene of miR-329, which is also high expressed in non-QCCSCs. Thereafter, we perform gain- and loss- function experiments to confirm the synergistic effect between miR-4666-3p and miR-329 in blocking the activation of TGF-β/Smad pathway. Finally, we evaluate the expression of both miR-4666-3p and miR-329 in 73 tumor specimens and paired normal tissue, and find both two miRNAs are related to unfavorable prognosis and advanced tumor stage in colorectal cancer. Our study revealed a novel epigenetic regulation mechanism in colon cancer stem cells, which could be exploited as a novel therapeutic strategy for cancer treatment.</p
4666. Chronique scandaleuse, ou médisante
No full text4666. Chronique scandaleuse, ou médisante. In: Molinier Auguste. Les Sources de l'histoire de France - Des origines aux guerres d'Italie (1494). V. Introduction générale - Les Valois (suite), Louis XI et Charles VIII (1461-1494) Paris : A. Picard et fils, 1904. pp. 24-25
4666. Chronique scandaleuse, ou médisante
No full text4666. Chronique scandaleuse, ou médisante. In: Molinier Auguste. Les Sources de l'histoire de France - Des origines aux guerres d'Italie (1494). V. Introduction générale - Les Valois (suite), Louis XI et Charles VIII (1461-1494) Paris : A. Picard et fils, 1904. pp. 24-25
The kinematics of the diffuse ionized gas in NGC 4666
Get PDFInternational audienceContext. The global properties of the interstellar medium with processes such as infall and outflow of gas and a large scale circulation of matter and its consequences for star formation and chemical enrichment are important for the understanding of galaxy evolution. Aims. In this paper we studied the kinematics and morphology of the diffuse ionized gas (DIG) in the disk and in the halo of the star forming spiral galaxy NGC 4666 to derive information about its kinematical properties. Especially, we searched for infalling and outflowing ionized gas. Methods. We determined surface brightness, radial velocity, and velocity dispersion of the warm ionized gas via high spectral resolution (R approximate to 9000) Fabry-Perot interferometry. This allows the determination of the global velocity field and the detection of local deviations from this velocity field. We calculated models of the DIG distribution and its kinematics for comparison with the measured data. In this way we determined fundamental parameters such as the inclination and the scale height of NGC 4666, and established the need for an additional gas component to fit our observed data. Results. We found individual areas, especially along the minor axis, with gas components reaching into the halo which we interpret as an outflowing component of the DIG. As the main result of our study, we were able to determine that the vertical structure of the DIG distribution in NGC 4666 is best modeled with two components of ionized gas, a thick and a thin disk with 0.8 kpc and 0.2 kpc scale height, respectively. Therefore, the enhanced star formation in NGC 4666 drives an outflow and also maintains a thick ionized gas layer reminiscent of the Reynold's layer in the Milky Way
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Linked collectors and determiners for: Tropical epiphyte diversity under human impact ? Comparing primary forests, secondary forests, and forest fragments in Ecuador - Bilsa.
No full textNatural history specimen data linked to collectors and determiners held within, "Tropical epiphyte diversity under human impact ? Comparing primary forests, secondary forests, and forest fragments in Ecuador - Bilsa". Claims or attributions were made on Bionomia by volunteer Scribes, <a href="http://bionomia.net/dataset/42962fd5-34ee-4666-abdb-04e8ba4d23b0">https://bionomia.net/dataset/42962fd5-34ee-4666-abdb-04e8ba4d23b0</a> using specimen data from the dataset aggregated by the Global Biodiversity Information Facility, <a href="https://gbif.org/dataset/42962fd5-34ee-4666-abdb-04e8ba4d23b0">https://gbif.org/dataset/42962fd5-34ee-4666-abdb-04e8ba4d23b0</a>. Formatted as a Frictionless Data package
Linked collectors and determiners for: Tropical epiphyte diversity under human impact ? Comparing primary forests, secondary forests, and forest fragments in Ecuador - Bilsa.
No full textNatural history specimen data linked to collectors and determiners held within, "Tropical epiphyte diversity under human impact ? Comparing primary forests, secondary forests, and forest fragments in Ecuador - Bilsa". Claims or attributions were made on Bionomia by volunteer Scribes, <a href="http://bionomia.net/dataset/42962fd5-34ee-4666-abdb-04e8ba4d23b0">https://bionomia.net/dataset/42962fd5-34ee-4666-abdb-04e8ba4d23b0</a> using specimen data from the dataset aggregated by the Global Biodiversity Information Facility, <a href="https://gbif.org/dataset/42962fd5-34ee-4666-abdb-04e8ba4d23b0">https://gbif.org/dataset/42962fd5-34ee-4666-abdb-04e8ba4d23b0</a>. Formatted as a Frictionless Data package
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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