1,724,123 research outputs found
CREBBP is a novel target of miR-4474/4717.
No full text(A) The sequences of miR-4474/4717 and the potential binding site in the 3’UTR of CREBBP. (B and E) Luciferase reporter assay. HEK293 cells were transiently cotransfected with 0.8 μg of pMIR-CREBBP-wt or pMIR-CREBBP-mut, 0.04 μg of Renilla luciferase control vector, and 100 nM miR-4474/miR-4717 control, mimic, or inhibitor with Lipofectamine 2000 for 24 h. (C,F, D and G) Western blot and qRT-PCR analyses. Human epithelial colorectal Caco-2 cells were transfected with miR-4474/4717 control, mimic or inhibitor for 24 h, and the mRNA and protein expression of CREBBP was detected by qRT-PCR and Western blot analyses. The results shown are representative of at least three independent experiments. *P< 0.05.</p
Recurrent cystitis and bladder mass in two adults with chronic granulomatous disease
Get PDFContains fulltext :
4474.pdf (Publisher’s version ) (Open Access
Linked collectors and determiners for: Notes on Shore Flies (Diptera: Ephydridae) from Finland and north-western Russia.
No full textNatural history specimen data linked to collectors and determiners held within, "Notes on Shore Flies (Diptera: Ephydridae) from Finland and north-western Russia". Claims or attributions were made on Bionomia by volunteer Scribes, <a href="http://bionomia.net/dataset/e6839e1e-336f-4474-b8fa-085a3894358c">https://bionomia.net/dataset/e6839e1e-336f-4474-b8fa-085a3894358c</a> using specimen data from the dataset aggregated by the Global Biodiversity Information Facility, <a href="https://gbif.org/dataset/e6839e1e-336f-4474-b8fa-085a3894358c">https://gbif.org/dataset/e6839e1e-336f-4474-b8fa-085a3894358c</a>. Formatted as a Frictionless Data package
Linked collectors and determiners for: The Davidson College Entomology Collection.
No full textNatural history specimen data linked to collectors and determiners held within, "The Davidson College Entomology Collection". Claims or attributions were made on Bionomia by volunteer Scribes, <a href="http://bionomia.net/dataset/a4899898-3fb7-4474-85bf-be8975e6ba12">https://bionomia.net/dataset/a4899898-3fb7-4474-85bf-be8975e6ba12</a> using specimen data from the dataset aggregated by the Global Biodiversity Information Facility, <a href="https://gbif.org/dataset/a4899898-3fb7-4474-85bf-be8975e6ba12">https://gbif.org/dataset/a4899898-3fb7-4474-85bf-be8975e6ba12</a>. Formatted as a Frictionless Data package
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Alteration of microRNA-4474/4717 expression and CREB-binding protein in human colorectal cancer tissues infected with Fusobacterium nucleatum.
No full textColorectal cancer (CRC) is a common and highly lethal form of cancer. Although the etiologic role of Fusobacterium nucleatum (F. nucleatum) in the development of CRC has been elucidated, the specific tumor molecules involved in the progression of CRC induced by F. nucleatum have not been identified. This study investigated several miRNAs and genes involved in the progression of F. nucleatum-induced CRC by Affymetrix miRNA microarray technology and GeneChip Human Transcriptome Array 2.0. The results suggest that miR-4474 and miR-4717 are up-regulated in CRC tissues in response to F. nucleatum infection, compared with the control group (paracancerous tissues), while other genes associated with signaling pathways in cancer, including CREB-binding protein (CREBBP), STAT1, PRKACB, CAMK2B, JUN, TP53 and EWSR1, were dysregulated. Bioinformatic analysis identified CREBBP as the primary aberrantly expressed gene in F. nucleatum-induced CRC. Consistent with the microarray analysis results, real-time RT-PCR analysis demonstrated that the expression of miR-4474/4717 was upregulated while that of CREBBP mRNA was downregulated in CRC patients infected with F. nucleatum. Additionally, CREBBP was identified as a novel target of miR-4474/4717. The results of this study suggest that miR-4474 and miR-4717 are involved in the progression of F. nucleatum-induced CRC by posttranscriptionally regulating the target gene CREBBP
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
- …
