1,725,199 research outputs found
Astronomische Nachrichten, t. 187, nos 4465-4488
No full textF. G. Astronomische Nachrichten, t. 187, nos 4465-4488. In: Bulletin astronomique, tome 30, 1913. pp. 334-360
Mechanism of miR-4465 targeting PTEN-mediated autophagy of astrocytes in epilepsy
Full text linkBackground: To investigate the mechanism of miR-4465 targeting PTEN-mediated autophagy of astrocytes in epilepsy. Methods: Serum samples were collected from epileptic children and healthy children. Extract foreign bodies from serum samples and determine their quality. The exosomes were sequenced, and the abnormal expression of miRNA in patients' serum exosomes was analysed, and the expression of miR-4465 was verified by quantitative PCR. Bioinformatics predicts the size of miR-4465 and makes GO and KEGG analyses. HEK293 cells were cultured, and the relationship between miR-4465 and its target was detected using the double luciferase reporting method. Astrocytes were cultured, and quantitative PCR and WB were used to detect the expression of miR-4465 and PTEN after overexpression. In addition, CCK-8 and WB were used to detect the growth of miR-4465 and the changes of autophagy-related proteins ATG5 and Beclin1, respectively. Results: miR-4465 was markedly increased in exosomes. The bioinformatic analysis found the differentially expressed target genes of miR-4465 were mainly enriched in molecular binding, molecular function regulation, and other molecular functions and participated in cell adhesion, cell-extracellular matrix receptor interaction, and the Rap1 signalling pathway. PTEN has been predicted as a target gene of miR-4465; meanwhile, the results of the dual-luciferase reporter assay confirmed the interaction between miR-4465 and PTEN. Quantitative PCR, as well as WB results, suggested the level of PTEN was decreased in serum exosomes of patients with epilepsy, while increased miR-4465 expression inhibited expressions of PTEN. CCK-8, as well as WB results, suggested miR-4465 could suppress the growth of astrocytes and promote ATG5 as well as Beclin1 expression; finally, up-regulation of PTEN partially alleviated effects of miR-4465 on astrocytes growth as well as autophagy. Conclusions: In children with epilepsy, miR-4465 can target and regulate PTEN to promote autophagy in astrocytes
LncRNA THUMPD3-AS1/microRNA-4465/KPNA2 axis impacts human hepatocellular carcinoma cell phenotypes
No full textObjective: Hepatocellular carcinoma (HCC) is a lethal malignancy in the world. LncRNA THUMPD3-AS1 is implicated in tumorigenesis and progression in various tumors. Therefore, this study was applied to investigate the action of THUMPD3-AS1 in HCC by regulating microRNA (miR)-4465 and KPNA2. Methods: The clinical specimens of HCC were collected to determine THUMPD3-AS1, KPNA2, miR-4465, E-cadherin, Vimentin, N-cadherin, ZEB1 and SNAIL levels. HCC cells were screened and transfected with sh-THUMPD3-AS1 or miR-4465 mimic to explore their roles in HCC cell phenotype and epithelial-mesenchymal transition (EMT)-related factors. The involvement of miR-4465 in THUMPD3-AS1-mediated HCC was proved. The relationship of THUMPD3-AS1, KPNA2 and miR-4465 was verified. Results: Overexpressed THUMPD3-AS1 and KPNA2 and reduced miR-4465 were present in HCC clinical tissues. THUMPD3-AS1 bound to miR-4465 to target KPNA2. Silencing of THUMPD3-AS1 or restoration of miR-4465 repressed HCC cell phenotypes and EMT in vitro. Inhibition of miR-4465 mitigated the role of silenced THUMPD3-AS1 in HCC. Conclusion: This study stresses that THUMPD3-AS1 induces EMT in HCC cells and ultimately promotes HCC cell growth and migration by competitively inhibiting miR-4465 expression and thus upregulating KPNA2
Nueva Lectura de un Epígrafe de Isona (CIL II 4465)
Full text linkEn el interior de la iglesia de Isona (Lérida) se encuentra una interesante inscripción que, mencionada desde antiguo por diversos autores, fue definitivamente catalogada por E. Hübner en CIL II, 4465
Nueva Lectura de un Epígrafe de Isona (CIL II 4465)
Full text linkEn el interior de la iglesia de Isona (Lérida) se encuentra una interesante inscripción que, mencionada desde antiguo por diversos autores, fue definitivamente catalogada por E. Hübner en CIL II, 4465
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
III/4465 Horka nad Moravou, part: street Náměstí Osvobozen
No full textNáplní bakalářské práce je úprava křižovatky ulic Náměstí osvobození, P.Bezruče a 1.máje v obci Horka nad Moravou. Práce obsahuje úpravu zpevněných ploch a zklidnění silnice III/4465 v prostoru ulice Náměstí osvobození. Tím by se měla zpomalit průjezdní rychlost vozidel a zvýšit bezpečnost silničního provozu a chodců. Práce obsahuje řešení statické dopravy, autobusových zastávek, úpravy a napojení chodeckých tras a veřejného osvětlení.The subject of my bachelor thesis is adjustment of crossroad of streets Náměstí osvobození, P.Bezruče and 1.máje. The thesis contain adjustment of strengthened places and calm road III/4465 in space of street Náměstí osvobození. That should decrease speed of vehicles and increase safety of walkers. The thesis contain solving of static traffic, bus stop, adjustment and connection walker line and public lightening.
III/4465 Horka nad Moravou, part: street Náměstí Osvobozen
No full textThe subject of my bachelor thesis is adjustment of crossroad of streets Náměstí osvobození, P.Bezruče and 1.máje. The thesis contain adjustment of strengthened places and calm road III/4465 in space of street Náměstí osvobození. That should decrease speed of vehicles and increase safety of walkers. The thesis contain solving of static traffic, bus stop, adjustment and connection walker line and public lightening
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