1,728,115 research outputs found
Tumor growth and metastasis associated with miR-4270 <i>in vivo</i>.
No full text(A–B) Levels of miR-4270 in five NSCLC cell lines (left panel), stable miR-4270-knockdown A973 cells (Lenti-Inhibitor), and control A973 cells (Lenti-Control) (right panel). (C) Tumor volumes in mice subcutaneously implanted with stable miR-4270-knockdown A973 cells (left panel) and representative images of endpoint tumor burden, as determined by in vivo luciferase imaging (right panel). (D) Representative H&E-stained images and Ki-67 IHC-stained images of the miR-4270-knowckdown xenograft. (E) Representative images of tumor burden, as determined by in vivo luciferase imaging of the lung, brain, liver, adrenal gland, and bone. Endpoint H&E staining results for mice subjected to the tail vein injection of stable miR-4270-knockdown A973 cells. Data are presented as the means ± SD of three experiments. ** P P < 0.05.</p
[Veto of H. 4270, R-179]
No full textThis veto message from Governor Mark Sanford vetoes H. 4270, R-179, a bill providing that beginning with school year 2003-2004, the starting date for the annual school term of Chester, Fairfield, Florence and Union County School Districts must be set by the board of trustees of the respective school district
Linked collectors and determiners for: Phylogenetic analysis and revision of the leafhopper genus Acuera DeLong & Freytag (Hemiptera: Cicadellidae: Gyponini) based on morphological data.
No full textNatural history specimen data linked to collectors and determiners held within, "Phylogenetic analysis and revision of the leafhopper genus Acuera DeLong & Freytag (Hemiptera: Cicadellidae: Gyponini) based on morphological data". Claims or attributions were made on Bionomia by volunteer Scribes, <a href="http://bionomia.net/dataset/6ba6d2ca-5dad-4270-914f-1042327d503c">https://bionomia.net/dataset/6ba6d2ca-5dad-4270-914f-1042327d503c</a> using specimen data from the dataset aggregated by the Global Biodiversity Information Facility, <a href="https://gbif.org/dataset/6ba6d2ca-5dad-4270-914f-1042327d503c">https://gbif.org/dataset/6ba6d2ca-5dad-4270-914f-1042327d503c</a>. Formatted as a Frictionless Data package
DataSheet_1_MiR-4270 acts as a tumor suppressor by directly targeting Bcl-xL in human osteosarcoma cells.pdf
No full textChondrosarcomas and osteosarcomas are malignant bone tumors with a poor prognosis when unresectable or metastasized. Moreover, radiotherapy and chemotherapy could be ineffective. MiRNAs represent an alternative therapeutic approach. Based on high-throughput functional screening, we identified four miRNAs with a potential antiproliferative effect on SW1353 chondrosarcoma cells. Individual functional validations were then performed in SW1353 cells, as well as in three osteosarcoma cell lines. The antiproliferative and cytotoxic effects of miRNAs were evaluated in comparison with a positive control, miR-342-5p. The cytotoxic effect of four selected miRNAs was not confirmed on SW1353 cells, but we unambiguously revealed that miR-4270 had a potent cytotoxic effect on HOS and MG-63 osteosarcoma cell lines, but not on SaOS-2 cell line. Furthermore, like miR-342-5p, miR-4270 induced apoptosis in these two cell lines. In addition, we provided the first report of Bcl-xL as a direct target of miR-4270. MiR-4270 also decreased the expression of the anti-apoptotic protein Mcl-1, and increased the expression of the pro-apoptotic protein Bak. Our findings demonstrated that miR-4270 has tumor suppressive activity in osteosarcoma cells, particularly through Bcl-xL downregulation.</p
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Putative target genes of miR-4270 and miR-423-3p.
No full text(A–B) The predicted miR-4270 binding site in the 3’-UTR of MMP-19 (left panel), and the predicted miR-423-3p binding site in the 3’-UTR of P21 (right panel). (C–D) Quantification of (C) MMP-19 mRNA expression and (D) P21 mRNA expression after the transfection of the miRNA mimic or inhibitor. (E–F) Quantification of (E) MMP-19 protein expression and (F) P21 protein expression after the transfection of the miRNA mimic or inhibitor. (G–H) Results of luciferase reporter assay, after (G) the co-transfection of cells with miR-4270 and the pGL3 construct containing MMP19 with either a WT or a MT 3’-UTR region, or (H) the co-transfection of cells with miR-423-3p and the pGL3 construct containing P21 with either a WT or a MT 3’-UTR region. Results were normalized to those of cells co-transfected with the negative control mimic (NC) and the pGL3 construct containing either a WT or a MT MMP19 or P21. (I–J) Immunohistochemistry of MMP-19 and P21 in the tumor tissues of nude mice treated with (I) the miR-4270-knockdown xenograft or (J) the miR-423-3p-overexpression xenograft. Data are presented as the means ± SD of three experiments. ** P < 0.01.</p
MiR-4270 acts as a tumor suppressor by directly targeting Bcl-xL in human osteosarcoma cells
No full textInternational audienceChondrosarcomas and osteosarcomas are malignant bone tumors with a poor prognosis when unresectable or metastasized. Moreover, radiotherapy and chemotherapy could be ineffective. MiRNAs represent an alternative therapeutic approach. Based on high-throughput functional screening, we identified four miRNAs with a potential antiproliferative effect on SW1353 chondrosarcoma cells. Individual functional validations were then performed in SW1353 cells, as well as in three osteosarcoma cell lines. The antiproliferative and cytotoxic effects of miRNAs were evaluated in comparison with a positive control, miR-342-5p. The cytotoxic effect of four selected miRNAs was not confirmed on SW1353 cells, but we unambiguously revealed that miR-4270 had a potent cytotoxic effect on HOS and MG-63 osteosarcoma cell lines, but not on SaOS-2 cell line. Furthermore, like miR-342-5p, miR-4270 induced apoptosis in these two cell lines. In addition, we provided the first report of Bcl-xL as a direct target of miR-4270. MiR-4270 also decreased the expression of the anti-apoptotic protein Mcl-1, and increased the expression of the pro-apoptotic protein Bak. Our findings demonstrated that miR-4270 has tumor suppressive activity in osteosarcoma cells, particularly through Bcl-xL downregulation
Rescue assay of miR-4270 and miR-423-3p.
No full text(A) The mRNA and protein expression levels of MMP-19. (B) The results of the migration assay after the co-transfection of cells with the miR-4270 mimic and the pEGFP-C1 plasmid containing an MMP-19 CDS sequence. (C) MMP-19 protein expression, as measured by immunohistochemical staining, in LAD samples. (D) Spearman correlation analysis showing the negative correlation between MMP-19 and miR-4270 expression. (E) The mRNA and protein expression levels of P21. (F) The results of the migration assay after the co-transfection of cells with the miR-423-3p mimic and pEGFP-C1 plasmid containing a P21 CDS sequence. (G) P21 protein expression, as measured by immunohistochemical staining, in LAD samples. (H) Spearman correlation analysis showing the negative correlation between P21 and miR-423-3p expression. Data are presented as the means ± SD of three experiments. ** P < 0.01.</p
Multivariate cox regression analysis between miR-4270 expression and BM LAD patients.
No full textMultivariate cox regression analysis between miR-4270 expression and BM LAD patients.</p
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