1,720,959 research outputs found
Complete regulation of glioma stem cell by notch and Akt signal inhibition
Full text link膠芽腫は予後不良な悪性腫瘍であり、膠芽腫幹細胞に対する治療が再発予防や根治性の向上に重要とされている。我々は、Notch阻害薬はAktシグナル抑制によって膠芽腫幹細胞へ効果を示すことと高感受性群と低感受性群に分けられることを報告した。本研究では、Notch阻害剤低感受性膠芽腫幹細胞群に対しNotch阻害剤とAkt阻害剤の併用療法の有効性を評価した。2剤併用療法は単剤よりも有意に細胞増殖抑制、幹細胞形質維持の抑制、アポトーシス誘導の増強を認めた。したがってNotch阻害薬にAkt阻害薬を併用することによりNotch阻害剤低感受性膠芽腫幹細胞を制御できることは示唆された。Glioblastoma is the most malignant primary brain tumor in humans. The patinets with glioblastoma have a uniformly poor prognosis. The significance of targeting glioblastoma-stem cells may be important for successful treatment. In this study, we analyzed the efficacy of the Nothc inhibitor and Akt inhibitor for glioblastoma stem cell with Notch inhibitor resistance. Notch inhibitor concomitant Akt inhibitor treatment enhanced the effect by monothearapy such as suppression of cell proliferation and stemness maintenance, and promotion of apoptosis.研究課題/領域番号:15K19956, 研究期間(年度):2015-04-01 - 2017-03-31出典：「Notch, Aktシグナル阻害によるグリオーマ幹細胞の完全制御」研究成果報告書 課題番号15K19956
（KAKEN：科学研究費助成事業データベース（国立情報学研究所））
（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-15K19956/15K19956seika/）を加工して作成金沢大学医薬保健研究域医学系research repor
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
The effect of the Notch inhibitor and a preclinical study for glioma stem cells
Full text link膠芽腫は予後不良な悪性脳腫瘍であり、膠芽腫幹細胞に対する治療が再発予防や根治性の向上に重要とされている。本研究では、9種類の膠芽腫患者由来膠芽腫幹細胞に対しNotch阻害剤MRK-003の有効性を評価した。MRK-003は全ての膠芽腫幹細胞に対し、細胞増殖抑制、アポトーシス促進、幹細胞形質抑制の効果を示した。使用した膠芽腫幹細胞は高感受性群と低感受性群に分けられた。IC50(50%阻害濃度）はCD44の発現量と負の相関関係、CD133の発現量と正の相関関係を認めた。MRK-003はCD44高値、CD133低値の膠芽腫幹細胞に有効であることが示唆された。Glioblastoma is the most malignant primary brain tumor in humans. The patients with glioblastoma have a uniformly poor prognosis. The significance of targeting glioblastoma-initiating cells (GICs) may be important for successful treatment of glioblastoma. In this study, we analyzed the effect of the Notch inhibitor MRK-003 on nine patient-derived GICs. MRK-003 suppressed GIC's proliferation and stemness maintenance, and promoted their apoptosis. Based on their sensitivities to MRK-003, the nine GICs were divided into “relatively sensitive” and “relatively resistant” GICs. The IC50(half maximal inhibitory concentration) of MRK003 in a set of GICs exhibited a negative correlation with CD44 and positive correlation with CD133. Our study suggested that MRK-003 may exhibit significant therapeutic potential for GICs with CD44-high and CD133-low expression.研究課題/領域番号:25861263, 研究期間(年度):2013-04-01 - 2015-03-31出典：研究課題「グリオーマ幹細胞に対するNotch阻害剤の効果解析と前臨床試験」課題番号25861263
（KAKEN：科学研究費助成事業データベース（国立情報学研究所））
（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-25861263/25861263seika/)を加工して作成research repor
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
The regulation of glioma stem cell by the transcription factor "RBPJ" suppression in Notch signal
Full text link膠芽腫は原発性悪性脳腫瘍であり予後不良な難治性の疾患である。本研究は膠芽腫幹細胞に対する標的治療研究である。我々はNotchシグナルの活性指標となる核内転写因子RBPJに着目した。膠芽腫組織ではRBPJの発現が正常脳より高いこと、膠芽腫幹細胞は分化型膠芽腫細胞よりもRBPJの発現が高いことを確認した。膠芽腫幹細胞のRBPJ抑制により細胞増殖低下、幹細胞形質喪失を認めた。メカニズムとしてRBPJの阻害により下流因子のインターロイキン6とSTATシグナルの抑制が誘導されたこと突き止めた。動物実験においても腫瘍の縮小を認めた。膠芽腫幹細胞制御にはRBPJの阻害が有効であると示唆された。Glioblastoma is a primary malignant brain tumor and is an intractable disease with a poor prognosis. This study is a targeted therapeutic study against glioblastoma stem cells. We focused on the nuclear transcription factor RBPJ, which is an active indicator of Notch signaling. RBPJ expression was higher in glioblastoma tissue than in normal brain, and glioblastoma stem cells had higher RBPJ expression than differentiated glioblastoma cells. RBPJ inhibition of glioblastoma stem cells results in decreased cell proliferation and loss of stem cell phenotype. As a mechanism, we found that inhibition of RBPJ induced inhibition of the downstream factors interleukin 6 and STAT signal. Tumor reduction was also checked in animal studies. It suggested that inhibition of RBPJ is effective for the regulation of glioblastoma stem cells.研究課題/領域番号:17K10858, 研究期間(年度):2017-04-01 - 2020-03-31出典：「Notchシグナルの核内転写因子RBP-Jk抑制によるグリオーマ幹細胞の制御」研究成果報告書 課題番号17K10858
（KAKEN：科学研究費助成事業データベース（国立情報学研究所））
（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-17K10858/17K10858seika/)を加工して作成金沢大学医薬保健研究域医学系research repor
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
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