1,720,971 research outputs found
Identification of Novel Treatment Targets and Biomarkers for Small-Cell Lung Cancer by Circulating Tumor Cell Analysis
Full text link【CTCを用いた遺伝子発現解析】小細胞肺癌12例の末梢血単核球分画からRNAを抽出し、RT-PCR法にてCTC由来遺伝子発現の解析を行った。神経内分泌関連および上皮間葉移行関連、血管新生関連の遺伝子発現を検討し、上皮間葉移行関連遺伝子発現を検出することができた。本方法でCTC関連遺伝子発現を検出できることが示唆された。
【血中DNAを用いた遺伝子変異解析】進行期肺癌剖検例4例を対象に、腫瘍組織由来のDNAと血中DNAを用いて、次世代シークエンサーにて遺伝子変異を解析した。血中DNA中の遺伝子変異は、複数病変に共通して存在し腫瘍組織内のallele frequencyが有意に高かった。[Gene expression analysis using circulating tumor cells (CTCs)] We extracted RNA from the peripheral blood mononuclear cell fraction of 12 small-cell lung cancer patients and analyzed it for CTC-derived gene expression by the reverse transcriptase-polymerase chain reaction (RT-PCR). We investigated neuroendocrine-related, epithelial-mesenchymal-transition-related, and angiogenesis-related gene expression and were able to identify epithelial-mesenchymal transition-related gene expression. The results suggested that it is possible to identify CTC-related gene expression by this method.
[Gene mutation analysis using blood DNA] We analyzed tumor tissue derived DNA and blood DNA from 4 autopsy cases of advanced lung cancer by using a next generation sequencer. The same gene mutations as in the blood DNA were present in multiple lesions, and allele frequency in the tumor tissue was significantly higher.研究課題/領域番号:26430142, 研究期間(年度):2014-04-01 - 2017-03-31出典：「血中循環がん細胞解析を利用した小細胞肺癌の新規治療標的・バイオマーカーの同定」研究成果報告書 課題番号26430142
（KAKEN：科学研究費助成事業データベース（国立情報学研究所））
（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-26430142/26430142seika/）を加工して作成金沢大学医薬保健研究域医学系research repor
Going Beyond Counting First Authors in Author Co-citation Analysis
Full text linkThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
VEGFR2 tyrosine kinase inhibitor (VEGFR-TKI)-resistant cancer tissue from a cancer-bearing mouse model
Full text linkマウス担癌モデルを用いた VEGFR2チロシンキナーゼ阻害薬(VEGFR2-TKI)耐性がん組織の回収を行った。VEGFR2-TKIである Ki8751(投与なし, 10 mg/kg/日,20 mg/kg/日)を投与し、縮小後に再増大した時点で腫瘍組織を回収し、耐性後組織とした。また組織回収時には血漿も回収した。10mg/kg/日群では 6匹中 3匹から 8週および 10週投与後に耐性後組織を回収することができた(以下耐性群)。耐性群と投与なし群の腫瘍組織から RNAを抽出し、血管新生関連因子(VEGFA, MET, HGF, IL-8, FGF7, KDR, EGF, FGF1, FGF2, KIT, VEGFB, VEGFC)の遺伝子発現を測定した。そのうち IL-8で耐性後に遺伝子発現が増加し、VEGFR2, HGFでは減少した。IL-8の血漿中濃度を ELISA法を用いて測定したところ、耐性群では 40.06pg/mLで投与なし群では 26.90 pg/mLであり、耐性群で上昇していた。IL-8は、VEGFR2-TKI耐性メカニズムに関わることが予想される。We collected VEGFR2 tyrosine kinase inhibitor (VEGFR2-TKI)-resistant cancer tissue from a cancer-bearing mouse model. We divided themice into groups that were given the VEGFR2-TKI Ki8751 in a dose of 10 mg/kg/day and20 mg/kg/day and an untreated control group, and after it had regressed, we collectedtumor tissue from the Ki8751 groups and used it resistant tissue. Blood plasma was collected at the time the tissue was collected. It was possible to collect resistant tissue from 3 (resistant group) of the 6 mice in the 10 mg/kg/day group, at 8 weeks after the start ofadministration. We extracted RNA from the tumor tissue of the resistant group and the control group and measured gene expression of angiogenesis-related factors (VEGFA, MET, HGF, IL-8, FGF7, KDR, EGF, FGF1, FGF2, KIT, VEGFB, VEGFC). Expression of one of the genes, IL-8, had increased after resistance developed, and expression of two of the genes, VEGFR2 and HGF, had decreased. Measurement of the plasma concentration of IL-8 by an ELISA yielded a concentration of 40.06 pg/mL in the resistant group and 26.90pg/mL in the control group, and the concentration in the resistant group was elevated. IL-8is suspected of being related to the mechanism of VEGFR2-TKI resistance.研究課題/領域番号:23701089, 研究期間(年度):2011-2012出典：研究課題「Vivo耐性株による血管新生阻害薬耐性メカニズム」課題番号23701089
（KAKEN：科学研究費助成事業データベース（国立情報学研究所））
（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-23701089/23701089seika/）を加工して作成金沢大学医薬保健研究域医学系research repor
Detection of tumor-derived mutations in circulating DNA
Full text linkEGFR遺伝子変異陽性非小細胞肺癌症例を本研究の対象とした。DNA回収量は血清のほうが多く、ダイレクトシークエンス法よりもScorpion ARMS法の方が、また血清よりも血漿を用いたほうが検出率は高かった。Whole genome amplification(WGA)を用いた検討ではWGAを加えたほうが検出感度は上昇した。問題点として、症例数が不十分であったことが挙げられる。課題として、さらに高感度の検出系の確立が必要である。Patients with EGFR mutations were enrolled in this study. The concentration of the DNA extracted was higher in the patients' serum than in their plasma. The rate of EGFR mutation detection was higher, in their DNA from plasma, and by the Scorpion ARMS method. The detection rate was higher when whole genome amplification was performed.A larger study and the establishment of a more sensitive assay is needed to achieve the purpose of this study.出典：研究課題「血中DNAを用いた腫瘍特異的遺伝子変異の検出とその臨床的意義」課題番号20790565
（KAKEN：科学研究費助成事業データベース（国立情報学研究所））
（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-20790565/20790565seika/）を加工して作成金沢大学医薬保健研究域医学系research repor
Variations on the Author
Full text link“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
血中遊離DNA遺伝子変異プロファイルを用いたがん患者モニタリング法の確立
Full text linkA total of 114 cfDNA samples at diagnosis and resistance to treatment were collected from 42 patients with advanced non-small cell lung cancer, and cfDNA was extracted. A gene mutation profile was created using the recovered cfDNA. We published a paper on the results of analysis of gene mutation mutation profiles using autopsy tissues (Koba H, Kimura H et al. Sci Rep. 2021). In the paper, it was shown that cfDNA is enriched with important gene mutations that characterize malignant tumors, and that the gene mutation profile in cfDNA changes over the course of treatment. These results suggest that cfDNA may better reflect the characteristics of the entire tumor compared to tumor tissue.進行期非小細胞肺癌患者42例から診断時および治療耐性時のcfDNAを計114検体回収しcfDNAを抽出した。回収できたcfDNAを用いて遺伝子変異プロファイルを作成した。我々は、剖検組織を用いた遺伝子変異変異プロファイルの解析結果について論文発表した(Koba H, Kimura H et al. Sci Rep. 2021)。論文の中で、cfDNA中には悪性腫瘍の特性を表す重要な遺伝子変異が濃縮されていること、治療経過でcfDNA中の遺伝子変異プロファイルは変化すること、を示した。これらの結果から、cfDNAは腫瘍組織と比べて、腫瘍全体の特性をより反映している可能性があることを提唱した。研究課題/領域番号:19K07727, 研究期間(年度):2019-04-01 - 2022-03-31出典：研究課題「血中遊離DNA遺伝子変異プロファイルを用いたがん患者モニタリング法の確立」課題番号19K07727
（KAKEN：科学研究費助成事業データベース（国立情報学研究所））
（https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-19K07727/19K07727seika/）を加工して作成金沢大学附属病院呼吸器内科research repor
Appropriate Similarity Measures for Author Cocitation Analysis
Full text linkWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Full text linkWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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