1,726,270 research outputs found
Proizvodnja transglutaminaze s pomocu Streptoverticillium ladakanumNRRL-3191 uzgojenom u mediju dobivenom odhidrolizata slame sirka
No full textThe aim of this work was to elucidate the suitability of the biotechnological produc-tion of transglutaminase byStreptoverticillium ladakanumNRRL-3191 grown on mediamade from hydrolysates of sorghum straw. Transglutaminase activity was determined infermentations on sorghum straw hydrolysates and commercial xylose with initial xylose10, 20 or 30 g/L. Using media containing commercial xylose 20 g/L, transglutaminase ac-tivity up to 0.282 U/mL was obtained in 96 h. Using neutralized, charcoal-treated hydro-lysates of sorghum straw with xylose 30 g/L sterilized in autoclave at 121 °C, up to 0.155U/mL was obtained in 96 h. However, when the sterilization was performed by filtration,using the same hydrolysates with xylose 20 g/L, up to 0.348 U/mL was obtained in 72 h.It was demonstrated that hydrolysates of sorghum straw are suitable media for transgluta-minase production byStreptoverticillium ladakanumA grant from the PROMEP program of the Secretaría de Educación Pública (México) to author Téllez-Luis is gratefully acknowledgedS
miR-3191 promotes the proliferation and metastasis of hepatocellular carcinoma via regulating PAK6
No full textAbstract Background/Aims microRNAs (miRNAs) contribute to tumorigenesis, progression and drug resistance of hepatocellular carcinoma (HCC). miR-3191 is a newly discovered miRNA, and its function and mechanism of action in biological processes and diseases are not completely understood. Methods miR-3191 expression is determined via quantitative real-time polymerase chain reaction. Knockdown and overexpression of miR-3191 influence the proliferation and metastasis of HCC cells, which is measured by Cell Counting Kit-8 assay, Colony Formation assay and Cell metastasis assay. Protein expression is estimated by Western blot. The interplay between miR-3191 and target is validated by dual-luciferase reporter assay. Results Here, we show that miR-3191 is upregulated in HCC tissues and associated with poor prognosis of HCC patients. Mechanistically, p21-activated protein kinase 6 (PAK6) was identified as a direct target of miR‑3191 in HCC. PAK6 knockdown partially recovered interference of miR‑3191‑induced decrease in cell proliferation and invasion. The accuracy of HCC patient prognosis could be improved by employing a combination of miR-3191 and PAK6 values. Conclusions miR-3191 promotes the proliferation and metastasis of HCC cells via targeting PAK6 and may serve as a prognostic biomarker and potential therapeutic target
Proizvodnja transglutaminaze iz Streptoverticillium ladakanum NRRL-3191 uz glicerol kao izvor ugljika
No full textThe enzyme transglutaminase (TG) catalyses the formation of covalent bonds between
adjacent proteins, thereby improving the gel structure of proteins and has important applications for the food industry. The aims of this work were: (i) to elucidate the effect of
agitation speed during the biotechnological production of TG by Streptoverticillium ladakanum NRRL-3191 using glycerol as carbon source; and (ii) to improve TG production by
optimising the composition of media based on glycerol, xylose and casein. An agitation
speed of 250 rpm and a fermentation time of 72 h resulted in the optimal enzymatic activity
(0.628 U/mL) with a productivity of 0.087 U/(mL·h). The composition of media with glycerol, xylose and casein were optimised using an experimental design to improve TG production. The model predicts that the maximum TG activity (0.725 U/mL) can be obtained
using glycerol 50.5 g/L and casein 20 g/L without the addition of xyloseA grant from the PROMEP program of the Secretaría
de Educación Pública (México) to author Téllez-Luis is
gratefully acknowledgedS
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
[IO Islamic 3191] خمسۀ نظامى
No full textKhamsa-i-Niżâmî.
This manuscript is now IO Islamic 977 in the India Office collections.
[metadata: Hermann Ethé, Catalogue of Persian Manuscripts in the Library of the India Office, 2 vols. (Oxford: India Office, 1903): volume 1, number 3191 here with notations and hyperlinks].
977
The same.
Contents:
1.Makhzan-alasrâr, on fol. 1b. The wrong date of A.H. 559 appears here on fol. 29b, l. 4: پانصد و پنجاه و نه. 2. Khusrau u Shîrîn, on fol. 30b. 3. Lailâ u Majnûn, on fol. 125b. The date, A.H. 584, appears on fol. 130b, l. 11. 4. Haft Paikar, on fol. 185b. The date, A.H. 593, appears on fol. 257b, l. 5. 5a. The first part of the Iskandarnâma, styled here شرفنامه or اسكندرنامۀ برّى (see fol. 344b and fol. 345a in the colophon), on fol. 258b. The date, A.H. 597, appears on fol. 345b, l. 10.
5b. The second part of the Iskandarnâma, styled here اقبالنامه or سكندرنامۀ بحرى, on fol. 345b.
This excellent copy, dated A.H. 1072=A.D. 1661, 1662 (see fol. 257b), was received into the Library March 29, 1878.
No. 3191, ff. 390, 4 coll., each ll. 21; Nasta’lîḳ; an illuminated frontispiece at the beginning of each mathnawî; pictures on ff. 46a, 53a, 56a, 57b, 63b, 74b, 83a, 84a, 89b, 109b, 114b, 118a, 119b, 147a, 153a, 158b, 195a, 197a, 198b, 203a, 205a, 207b, 213a, 216a, 217b, 219a, 220a, 221b (damaged), 222b, 225b, 226a, 229a, 229b, 233b, 234a, 238b, 240a, 240b, 244a, 245a, 245b, 249b, 276a, 289b, 292a, 297a, 301a, 303b, 321b, 323a, and 341b; size, 10½ in. by 6¾ in
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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