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Evaluation of haptoglobin concentration in clinically healthy dairy cows: correlation between serum and milk levels
Full text linkThe study aimed to analyse serum haptoglobin concentrations by calving number and lactation phase in clinically healthy dairy cattle and to determine the correlation between concentrations in serum and milk samples. For this purpose, serum samples were collected from 228 clinically healthy cows after milking from 14 randomly selected herds. Additionally, milk samples were collected from 80 out of the 228 cows from the milking prior to blood collection. Both serum and milk samples were analysed with a commercial ELISA test. The effect of the calving number and lactation phase on mean serum haptoglobin concentrations was examined by Kruskal–Wallis/Jonckheere–Terpstra tests. After that, in a multivariate approach, a mixed-effects linear model was also performed. In addition, intraclass correlation coefficients were used to evaluate the correlation between concentrations in serum and milk samples. Results indicated a downward trend in haptoglobin concentrations throughout lactation, that was statistically significant when comparing fresh cows with the remaining lactation phases. The higher haptoglobin concentrations in fresh cows could be the result of inflammatory illnesses without clinical disease, stress or variations within the physiological state. Although previous studies showed some discrepancies, a good correlation between serum and milk concentrations has been observed in the present paper.This research isincluded in IN852B 2021/17 Conecta HUBs project (Digital certification of traceability through facilitating tools in the value chain of the dairy industry).S
Effect of β-estradiol on adipogenesis in a 3T3-L1 cell model of prelamin A accumulation
Full text linkThe accumulation of farnesylated prelamin A has been suggested as one of the mechanisms responsible for the loss of fat in type 2 familial partial lipodystrophy due to variants in the LMNA gene. In this rare disease, fat loss appears in women after puberty, affecting sex-hormone-dependent anatomical areas. This study investigated the impact of 17-β-estradiol on adipogenesis in murine preadipocytes subjected to a pharmacologically induced accumulation of farnesylated and non-farnesylated prelamin A. To induce the accumulation of non-farnesylated or farnesylated prelamin A, 3T3-L1 cells were treated with the farnesyltransferase inhibitor 277 or the methyltransferase inhibitor N-acetyl-S-farnesyl-l-cysteine methylester. Subsequently, the cells were induced to undergo adipocyte differentiation in the presence or absence of 17-β-estradiol. Prelamin A accumulation was assessed through immunofluorescence, while real-time PCR and Western blot techniques were used to quantify several adipogenic genes and evaluate protein levels, respectively. The results showed that 17-β-estradiol increased adipogenesis, although the combination of this hormone plus farnesylated prelamin A led to a reduction in the number of mature adipocytes and the expression of the different genes involved in adipogenesis. In conclusion, the influence of farnesylated prelamin A accumulation on adipogenesis manifested only in the presence of estradiol. These in vitro findings suggest a potential mechanism that could explain the characteristic phenotype in women suffering type 2 familial partial lipodystrophyThis research was funded by the Instituto de Salud Carlos III, ISCIII and the European Regional Development Fund, ERDF (grant number PI081449), an intramural grant from the Xunta de Galicia (GPC2014/036, ED341b 2017/19, ED431B 2020/37) and a research grant from the Asociación Española de Familiares y Afectados de Lipodistrofias (AELIP). A.F.-P. receives funding from the Fundación Alfonso Martín EscuderoS
Meibum Lipidomic Analysis in Evaporative Dry Eye Subjects
Full text linkMeibomian Glands (MG) are sebaceous glands responsible for the production of meibum, the main component of the Tear Film Lipid Layer (TFLL). The TFLL facilitates the spread of the tear film over the ocular surface, provides stability and reduces tear evaporation. Alterations in meibum composition lead to different ocular alterations like Meibomian Gland Dysfunction (MGD) and subsequent Evaporative Dry Eye (EDE). The aim of the present study was to investigate the composition and abundance of meibum lipids and their relationship with eyelid margin abnormalities, lipid layer patterns and MG status. The study utilizes a lipidomic approach to identify and quantify lipids in meibum samples using an Elute UHPLC system. This system considered all four dimensions (mass/charge, retention time, ion mobility and intensity) to provide the accurate identification of lipid species. Samples were categorized as healthy or low/no signs of alteration (group 1) or severe signs of alteration or EDE/MGD (group 2). The current investigation found differences in Variable Importance in Projection lipid abundance between both groups for the MGD signs studied. Changes in meibum composition occur and are related to higher scores in eyelid margin hyperaemia, eyelid margin irregularity, MG orifice plugging, MG loss and lipid layer pattern.Convocatoria de Axudas para o Uso das Infraestructuras de Investigación da Universidade de Santiago de Compostela, número 2021-AD039S
Análisis de costes del tratamiento del cáncer oral en hospitales de la comunidad autónoma de Galicia
No full textLos costes directos del tratamiento del cáncer oral tienen especial
interés a pesar de su incidencia relativamente baja comparada a la de
otros tipos de cáncer, ya que estos a menudo se detectan en estadios
avanzados, lo que exige tratamientos más complejos y costosos. Hasta
la fecha, se han publicado estudios sobre la carga económica del
cáncer oral solo en 6 países europeos (Italia, Holanda, Grecia, Francia,
Inglaterra y Alemania).
Objetivos
Efectuar una estimación de los costes directos asociados a los
procedimientos de diagnóstico, tratamiento y seguimiento del cáncer
oral en España
Tribological improvement of potential lubricants for electric vehicles using double functionalized graphene oxide as additives
Full text linkThe aim of the present research is to analyze thermophysical and tribological properties of PAO4 nanolubricants containing functionalized (GO-polyethyleneimine-oleic acid, GO-PEI-OA) and non-functionalized (GO) graphene oxide. Nanodispersions of these NPs were prepared, observing a better stability for the GO-PEI-OA nanolubricants. As for the tribological properties, significant improvements in coefficient of friction were obtained comparing with the base oil, being 28% for GO-PEI-OA (0.10 wt%). Concerning the anti-wear behavior, better results were obtained for GO-PEI-OA nanolubricants (0.15 wt%) with improvements of about 27%, 54% and 60% for the diameter, depth, and area of the wear track, respectively. Finally, Raman spectroscopy and surface roughness measurements were used to identify the tribological mechanisms of tribofilm formation and repairing.This research is supported by Xunta de Galicia (ED431C 2020/10 and ED431G 2019/03), by MCIN/AEI/10.13039/501100011033 through PID2020–112846RB-C22 and PID2019–107338RB-C62 projects and by the European Regional Development Fund. JMLdR is grateful for financial support through the Margarita Salas program, funded by MCIN/AEI/10.13039/501100011033 and "NextGenerationEU/PRTR". Furthermore, authors are also thankful to Repsol Lubricants for providing the PAO4 base oil and to RIAIDT-USC for its analytical facilities.S
Fine-tuning the use of moss transplants to map pollution by Potentially Toxic Elements (PTEs) in urban areas
Full text linkMosspheres are a kind of moss transplants which offer a novel approach for detecting atmospheric pollution using devitalized mosses, as they reflect the atmospheric deposition of certain elements and polycyclic hydrocarbons. However, due to the unique features of the mosspheres such as the low elemental concentrations in the cultured material, the data treatment needs to be different from that of conventional biomonitoring studies. In this article, our objectives are to identify the best parameter for expressing the levels of chemical elements accumulated by mosspheres, and to apply a recently developed method to assess the probability of pollution of each sample and of the study area. To do this, we used data from a study in which 81 mosspheres were exposed in a medium-sized city in southwestern Europe.
Comparing different pollution indices, we selected the enrichment rate (ER) as the most useful, as it is resilient to fluctuations in the initial concentrations and takes into account the time factor, allowing for greater comparability among studies. Then, we determined that the statistical distribution of the ERs of most elements fitted a normal distribution, showing that most samples did not differ significantly from the background concentrations for these elements. On the other hand, for Ni, Pb and Zn there was a subpopulation of samples above background values. In these cases, we determined the probability of pollution of each sample. Finally, we used indicator kriging to calculate the probability of pollution across the study area, identifying the polluted areas, which for some elements match the distribution of the main industries and highways, indicating that this is a suitable protocol to map elemental pollution in urban areas.This study was financially supported by Caixabank. Antón Vázquez-Arias, Pablo Giráldez, Jesús R. Aboal and J. Ángel Fernández acknowledge financial support provided by the Xunta de Galicia - Consellería de Educación e Ordenación Universitaria (Consolidation of Competitive Research Groups; GI-1252, ED431C 2020/19). Antón Vázquez-Arias is grateful to the Spanish Ministerio de Ciencia, Innovación y Universidades for a grant awarded within the Programa de Formacion de Profesorado Universitario (grant number FPU19/01989).S
Thrombolytic therapy based on lyophilized platelet-derived nanocarriers for ischemic stroke
Full text linkIntravenous administration of fibrinolytic drugs, such as recombinant tissue plasminogen activator (rtPA) is the standard treatment of acute thrombotic diseases. However, current fibrinolytics exhibit limited clinical efficacy because of their short plasma half-lives and risk of hemorrhagic transformations. Platelet membrane-based nanocarriers have received increasing attention for ischemic stroke therapies, as they have natural thrombus-targeting activity, can prolong half-life of the fibrinolytic therapy, and reduce side effects. In this study we have gone further in developing platelet-derived nanocarriers (defined as cellsomes) to encapsulate and protect rtPA from degradation. Following lyophilization and characterization, their formulation properties, biocompatibility, therapeutic effect, and risk of hemorrhages were later investigated in a thromboembolic model of stroke in mice.S
Genética de la disfunción cardíaca relacionada con la terapia del cáncer
Full text linkEl éxito en el tratamiento del cáncer está empañado por los
eventos adversos que tienen algunos fármacos sobre la
función cardíaca, lo que se ha denominado disfunción cardíaca
relacionada con la terapia del cáncer (DCRTC). El objetivo
principal de este proyecto es identificar marcadores genéticos
claves en el desarrollo de la DCRTC, buscando en última
instancia la predicción del riesgo de eventos cardíacos severos
en los pacientes con cáncer. Para ello utilizamos tres
enfoques genéticos distintos: secuenciación del exoma
completo, estudio de asociación del genoma completo, y estima
de riesgo poligénico. Nuestros hallazgos respaldan la hipótesis
de que la variación genética contribuye a la susceptibilidad
de la DCRTC, y nos ayudan a comprender mejor los
mecanismos que la originan
Instrucción 3/2024, de la Secretaría General relativa a la celebración de reuniones de órganos colegiados de la Universidade de Santiago de Compostela por medios telemáticos
No full textPublicado no Taboleiro electrónico o 30 de xullo de 2024
Dispositivo de género y prácticas de participación intermediada en entornos híbridos. Un estudio fenomenográfico de mujeres adolescentes de Galicia para repensar la competencia digital
No full textEsta investigación busca conocer de qué formas experimentan
las mujeres adolescentes gallegas
la participación intermediada por tecnologías digitales para la
producción de vídeo. Con ello, se
pretende comprender: cómo viven la producción de discurso
acerca de ser mujer interactuando
en redes híbridas; las experiencias de interacción que articulan;
las oportunidades de
participación que encuentra en ellas; las relaciones posibles
entre estas prácticas y la
competencia digital crítica que poseen; y quién y cómo actúa de
referente en este proceso. En
este informe se detalla el diseño, implementación y resultados
del estudio, así como se apuntan
elementos para la reflexión y discusión didáctica