1,721,214 research outputs found
KR-31543 reduces the production of proinflammatory molecules in human endothelial cells and monocytes and attenuates atherosclerosis in mouse model
No full textKR-31543, (2S, 3R, 4S)-6-amino-4-[N-(4-chlorophenyl)- N-(2-methyl-2H-tetrazol-5-ylmethyl) amino]-3,4-dihydro- 2-dimethyoxymethyl-3-hydroxy-2-methyl-2H-1-benz opyran is a new neuroprotective agent for ischemiareperfusion damage. It has also been reported that KR-31543 has protective effects on lipid peroxidation and H2O2-induced reactive oxygen species production. In this study, we investigated the anti-inflammatory and anti-atherogenic properties of KR-31543. We observed that KR-31543 treatment reduced the production of MCP-1, IL-8, and VCAM-1 in HUVECs, and of MCP-1 and IL-6 in THP-1 human monocytes. We also examined the effect of KR-31543 on monocytes migration in vitro. KR-31543 treatment effectively reduced the migration of THP-1 human monocytes to the HUVEC monolayer in a dose-dependent manner. We next examined the effects of this compound on atherogenesis in LDL receptor deficient (Ldlr -/-) mice. After 10 weeks of western diet, the formation of atherosclerotic lesion in aorta was reduced in the KR-31543-treated group compared to the control group. The accumulation of macrophages in lesion was also reduced in KR-31543 treated group. However, the plasma levels of total cholesterol, HDL, LDL, and triglyceride were not affected by KR-31543 treatment. Taken together, these results show that KR-31543 has anti-inflammatory properties on human monocytes and endothelial cells, and inhibits fatty streak lesion formation in mouse model of atherosclerosis, suggesting the potential of KR-31543 for the treatment for atherosclerosis. © 2012 by the Korean Society for Biochemistry and Molecular Biology
Estrés laboral y liderazgo directivo en la Institución Educativa N° 31543 Tupac Amaru, Azapampa- Chilca
Get PDFEl estudio que lleva por título “Estrés laboral y liderazgo directivo en la institución educativa N° 31543 Tupac Amaru, Azapampa- Chilca”, tuvo como objetivo: Establecer la relación entre el estrés laboral y el liderazgo directivo en la institución educativa N° 31543 Tupac Amaru, Azapampa- Chilca. En la metodología, se aseguró que el estudio es de tipo básica, correlacional, desarrollado bajo el enfoque del método científico y el método inductivo – deductivo, asimismo es de enfoque cuantitativo, de diseño no experimental. La población y muestra se conformó por 36 docentes de la institución educativa N° 31543 Tupac Amaru, Azapampa- Chilca, a quienes se les realizo una encuesta en la que se utilizó dos cuestionarios sobre el estrés laboral y el liderazgo directivo. Los resultados mostraron que, con respecto al estrés laboral en relación con el liderazgo directivo; bajo las dimensiones como: control sobre el trabajo se tiene un nivel de significancia de 0,845 y t calculada es mayor que la t teórica (9,21 > 1,98). Con la dimensión demandas laborales, se tiene un nivel de significancia de 0,851 y t calculada es mayor que la t teórica (9,45 > 1,98), y en la dimensión apoyo social se tiene un nivel de significancia de 0,863 y t calculada es mayor que la t teórica (9,96 > 1,98). En conclusión, SI, existe una relación directa y significativa entre el estrés laboral con el liderazgo directivo en la institución educativa N° 31543 Tupac Amaru, Azapampa- Chilca, con un nivel de significancia de 0,857 y t calculada es mayor que la t teórica (9,68 > 1,98)
Abstract 2865: Protection from chemotherapy-induced alopecia by cytotech lbs API 31543 in a multichemotherapy course model of chloroleukemia
No full textAbstract
Alopecia remains the one side-effect of chemotherapy for which there is no therapeutic intervention. We have recently developed a multi-course chemotherapy rat model of transplantable chloroleukemia (MIAC51) in which to study Chemotherapy-[[Unsupported Character - Codename ­]]Induced Alopecia (CIA). This model provides the opportunity to study CIA and the cancer cells throughout multiple courses of chemotherapy. In the present study, we investigated the effect of calcitriol in a proprietary delivery system, Cytotech Labs API 31543 to protect from CIA using the following chemotherapy regimens: cyclophosphamide; cyclophosphamide and doxorubicin; cyclophosphamide, doxorubicin and cytarabine; cyclophosphamide, paclitaxel and etoposide; doxorubicin, paclitaxel and etoposide. Animals were injected intraperitoneally with 1×105 MIAC51 on day 5. Thereafter, 0.2µg of API 31543 or vehicle control was applied topically over the head area daily starting on day 6 for 6 consecutive days for the first course. Rats were isolated for 6 hours. Subsequently, the treated area was cleaned with soap and water and rats were returned to their litters. For the first cycle, chemotherapy regimens were administered on day 13, intraperitoneally in a total volume of 0.1 mL. Animals positive for leukemic cells in smears were euthanized on day 23. On day 31, a second anagen cycle was induced by clipping the hair in the neck area on leukemia survivors. Animals were then treated with 0.2 µg API 31543 on day 40 for 6 days in the shaven area. Chemotherapy regimens were then administered on day 46. In both cycles, alopecia was recorded 10 days after the last dose of chemotherapy. Neonatal rats treated with 0.2µg of API 31543 in the head area exhibited localized protection. Similarly, in the adult rats 0.2µg of API 31543 exerted a localized protective effect at the site of application. In both groups, rats that received chemotherapy alone became totally alopecic. Pretreatment with API 31543 protected the hair follicles against chemotherapy without having an effect on the efficacy of chemotherapy treatment of leukemia. In summary, the results herein set a solid foundation for clinical investigation of API 31543.
Citation Format: {Authors}. {Abstract title} [abstract]. In: Proceedings of the 101st Annual Meeting of the American Association for Cancer Research; 2010 Apr 17-21; Washington, DC. Philadelphia (PA): AACR; Cancer Res 2010;70(8 Suppl):Abstract nr 2865.</jats:p
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
Aspergillus candidus CCRC 31543發酵米麴乙醇萃取物於體內抗氧化能力及護肝功效之研究
No full text本研究乃探討Aspergills candidus CCRC 31543發酵米麴乙醇萃取物(EEAC)對大鼠的安全性、抗氧化活性與護肝功效;同時分析EEAC及管餵不同劑量EEAC大鼠血清中terphenyl類抗氧化物的含量變化。
HPLC分析結果顯示，每克EEAC中之3, 3”-dihydroxyterphenyllin (3,3”-DHT)、3-hydroxyterphenyllin (3-HT)及candidusin B (CANB)含量分別為 50.90、9.77 及5.29 g。SD大鼠經管餵EEAC (0.2 及1 g/kg b.w.) 28天進行安全性測試，結果並不會造成大鼠死亡。各組大鼠之體重變化、器官相對及絕對重量及血清生化值(GOT、GPT、ALP、LDH、-GT、TG及Chol)皆無顯著性差異(p > 0.05)。正常對照組與兩組EEAC組的肝臟結構組織完整。樣品組大鼠肝臟之總抗氧化力(TEAC)、GSH含量與GST、GRd、GPx及Cat等酵素活性顯著(p < 0.05)上升，TBARs值顯著(p < 0.05)低於正常對照組(p < 0.05)，此結果證實EEAC不會影響大鼠正常生長，且不具肝毒性，同時具有降低大鼠肝臟脂質過氧化及提升體內抗氧化力及酵素活性的功效。
以CCl4 (0.5 ml /rat, 20 %)誘導慢性肝損傷的結果顯示，大鼠經管餵EEAC (0.2及1.0 g/kg) 28天後，能顯著(p<0.05)降低CCl4 所誘導肝臟血清之酵素(GOT、GPT、ALP、LDH及-GT)、TG、Chol含量及抑制脂質過氧化，但與腎臟相關的血清生化值(BUN、Crea及TBil)則無顯著差異。由肝臟組織病理切片中發現，EEAC能降低CCl4對大鼠誘發之淋巴球浸潤、空泡形成、細胞壞死及有絲分裂等肝損傷現象。此外EEAC亦可顯著(p < 0.05)提升CCl4誘導肝組織之抗氧化酵素(GST、GRd、GPx、Cat及SOD)活性、體內抗氧化物質(GSH、Vit-C與Vit-E)含量與TEAC。分析EEAC組大鼠血清中3,3”-DHT、3-HT及CANB含量，顯示terphenyl類物質會以free form方式存在於血清中。根據上述試驗結果推測，3,3”-DHT、3-HT及CANB應具有保護肝臟免受四氯化碳慢性損傷，提升大鼠肝組織抗氧酵素活性及抗氧化物質含量。表次
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表一、活性氧物質之主要來源………………………………………10
表二、肝毒性物質及相關肝損傷……………………………………18
表三、微生物產生之抗氧化物………………………………………25
表四、p-terphenyl衍生物之安全性研究…………………………….33
第一部分
表1-1. A. candidus CCRC 31543培養發酵米麴乙醇萃取物之3, 3”-DHT、3-HT 及CANB含量…………………………………59
表1-2. 管餵A. candidus CCRC 31543發酵米麴乙醇萃取物28天後大鼠肝臟之相對及絕對重量變化………………………………61
表1-3. 餵食A. candidus CCRC 31543發酵米麴乙醇萃取物14天之大鼠血清GOT、GPT、LDH、ALP及-GT的變化…………62
表1-4. 餵食A. candidus CCRC 31543發酵米麴乙醇萃取物14天之大鼠血清中TG、Chol、BUN、Crea及TBil值的變化………63
表1-5.餵食A. candidus CCRC 31543發酵米麴乙醇萃取物28天之大鼠血清GOT、GPT、LDH、ALP及-GT的變化……………64
表1-6. 餵食A. candidus CCRC 31543發酵米麴乙醇萃取物28天之大鼠血清中TG、Chol、BUN、Crea及TBil值的變化………65
表1-7.餵食A. candidus CCRC 31543發酵米麴乙醇萃取物大鼠血清中3, 3”- DHT、3-HT及CANB的含量………………………..70
表1-8. 管餵A. candidus CCRC 31543發酵米麴乙醇萃取物28天之大鼠血清中抗氧化物質濃度的影響……………………………71
表1-9.餵食A. candidus CCRC 31543發酵米麴乙醇萃取物28天對大鼠肝臟中抗氧化酵素活性之影響………………………………72
第二部分
表2-1. 餵食A. candidus CCRC 31543發酵米麴乙醇萃取物對管餵四氯化碳誘發慢性肝損傷之大鼠總增加體重及每日增加體重…99
表2-2. 餵食A. candidus CCRC 31543發酵米麴乙醇萃取物對管餵四氯化碳誘發慢性肝損傷之大鼠肝、腎臟之相對及絕對重量變化…………………………………………………………………100
表2-3.餵食A. candidus CCRC 31543發酵米麴乙醇萃取物對管餵四氯化碳誘發慢性肝損傷之第14天之大鼠血清GOT、GPT、LDH、ALP及-GT的變化…………………………………………… 101
表2-4.餵食A. candidus CCRC 31543發酵米麴乙醇萃取物對管餵四氯化碳誘發慢性肝損傷之第14天之大鼠血清中TG、Chol、BUN、Crea及TBil值的變化…………………………………102
表2-5.餵食A. candidus CCRC 31543發酵米麴乙醇萃取物對管餵四氯化碳誘發慢性肝損傷之第28天之大鼠血清GOT、GPT、LDH、ALP及-GT的變化………………………………………………103
表2-6.餵食A. candidus CCRC 31543發酵米麴乙醇萃取物對管餵四氯化碳誘發慢性肝損傷之第28天之大鼠血清中TG、Chol、BUN、Crea及TBil值的變化……………………………………104
表2-7. 餵食A. candidus CCRC 31543發酵米麴乙醇萃取物對管餵四氯化碳誘發慢性肝損傷之大鼠血清中3, 3”-DHT、3-HT 及CANB之影響……………………………………………………………108
表2-8.餵食A. candidus CCRC 31543發酵米麴乙醇萃取物對管餵四氯化碳誘發慢性肝損傷之大鼠血清中抗氧化物含量之影響…109
表2-9.餵食A. candidus CCRC 31543發酵米麴乙醇萃取物對管餵四氯化碳誘發慢性肝損傷之大鼠肝臟中抗氧化酵素活性之影響………………………………………………………………….110
表2-10. 餵食A. candidus CCRC 31543發酵米麴乙醇萃取物對管餵四氯化碳誘發慢性肝損傷之大鼠肝臟組織傷害程度之影響…112
圖次
文獻整理
圖一、動物粒線體中的電子傳遞鏈…………………………………11
圖二、氧化壓力與疾病之間的關係…………………………………13
圖三、肝臟組織………………………………………………………15
圖四、Silymarin類黃酮類化學結構式……………………………….21圖五、Aspergillus candidus叢孢及孢子示意圖………………………29
圖六、Aspergillus candidus：(A) CZ培養基25℃培養14天之菌落圖； (B) MEA培養基25℃培養14天之菌落圖；(C) 放大1180倍之叢孢圖 (D) 放大1650倍之孢子圖 (E) 孢子電顯圖……………..30
圖七、3’3”-DHT、3-HT及candidusin B之結構式……………………32
第一部分
圖1-1. A. candidus CCRC 31543培養發酵米麴乙醇萃取物之3, 3”-DHT、3-HT 及CANB含量………………………………..58
圖1-2. 管餵A. candidus CCRC 31543發酵米麴乙醇萃取物28天之大鼠體重變化……………………………………………………60
圖1-3.餵食A. candidus CCRC 31543發酵米麴乙醇萃取物對大鼠肝臟脂質過氧化之影響……………………………………………66
圖1-4.餵食A. candidus CCRC 31543發酵米麴乙醇萃取物對大鼠肝臟總抗氧化力之影響……………………………………………67
圖1-5. 管餵A. candidus CCRC 31543發酵米麴乙醇萃取物之大鼠血清中(A) 3,3”-DHT、(B) 3-HT及(C) CANB含量之高效能液相層析圖譜及全波長掃描……………………………………………68
圖1-6. 未餵食A. candidus CCRC 31543發酵米麴乙醇萃取物之大鼠血清中(A) 3,3”-DHT、(B) 3-HT及(C) CANB含量之高效能液相層析圖譜…………………………………………………………69
圖1-7. 餵食A. candidus CCRC 31543發酵米麴乙醇萃取物28天對大鼠肝臟組織切片圖……………………………………………73
第二部分
圖2-1.餵食A. candidus CCRC 31543發酵米麴乙醇萃取物對管餵四氯化碳誘發慢性肝損傷之大鼠肝臟脂質過氧化之影響………105
圖2-2.餵食A. candidus CCRC 31543發酵米麴乙醇萃取物對管餵四氯化碳誘發慢性肝損傷之大鼠血清脂質過氧化之影響………106
圖2-3.餵食A. candidus CCRC 31543發酵米麴乙醇萃取物對管餵四氯化碳誘發慢性肝損傷之大鼠肝臟總抗氧化力之影響……..107
圖2-4.餵食A. candidus CCRC 31543發酵米麴乙醇萃取物對管餵四氯化碳誘發慢性肝損傷之大鼠肝臟組織切片圖……………..11
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