1,720,961 research outputs found
New therapeutic agent for danon disease: reserch from patient specific iPSCs derived cardiomyocytes
Get PDF女性Danon病患者からiPS細胞(iPSC)の作製に成功した。しかし、LAMP2蛋白の免疫染色においてDanon病患者全てのiPSCが染色され、LAMP2陽性、陰性のiPSCに対する検討は行えなかった。
採取したcDNAに対しLAMP2のPCRを行ったところ、Exon6欠損Line(miPSC)と発現Line(ciPSC)を確認することが出来た。miPSCと、ciPSCにおいてiPSC、iPSC由来心筋様細胞に関して検討を行った。心筋様細胞の透過電子顕微鏡での形態評価では、miPSCでDanon病に特徴的な自己貪食空胞の存在が確認出来た。治療候補薬の投与と評価は行うことができず、期限となった。We manufactured hiPSCs from female patients of danon disease. Genomic sequencing of iPSCs were performed. We identified a 4-bp deletion in LAMP2 at the intron 6 splice site (IVS6+1_4delGTGA) in all lines of hiPSCs same as their whole blood as previously reported. However, we could not compare the differences between LAMP2 positive and negative iPSCs, because all of the iPSC lines were stained by LAMP2 antibody IF. cDNA sequence of LAMP2 was performed. Exon6 skipping was proved in some of the hiPSC lines (miPSCs) and the other lines(ciPSCs) had normal cDNA sequence of LAMP2.
We compared the difference between miPSCs and ciPSCs. The miPSCs derived cardiomyocytes had intracytoplasmic vacuoles which was thought to be autophagosome: the characteristics of danon disease. On the contrary, we could not find any abnormal vacuole in the cytoplasm of ciPSCs. We could not administer and make evaluation of the candidate therapeutic agent because of expiration of the term.研究課題/領域番号:26860786, 研究期間(年度):2014-04-01 - 2018-03-31出典：研究課題「Danon病iPS細胞由来心筋・骨格筋細胞の解析と治療薬効果の評価」課題番号26860786
（KAKEN：科学研究費助成事業データベース（国立情報学研究所））
（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-26860786/26860786seika/）を加工して作成金沢大学附属病院循環器内科research repor
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
koamabayili/VECTRON-author-checklist: VECTRON author checklist
No full textWe have done our best to complete the author checklist relating to the use of animals in the hut study. Note that the objective for the hut study was to evaluate the IRS treatment applications for residual efficacy against Anopheles mosquitoes, including the local An. coluzzii mosquito population. Cows were only used to attract mosquitoes into the huts and no tests were carried out directly on the cows. The author checklist is intended for use with studies where experiments are carried out on animals, which is why we have had such difficulty in completing this for the hut study, as many of the questions do not relate to how the cows were used
女性ダノン病iPS細胞由来心筋を用いたXISTの役割と遺伝子治療の可能性について
Get PDF難治性心筋疾患であるダノン病の女性患者よりiPS細胞を樹立し、心筋細胞へ分化させた。分化した心筋はダノン病特有の表現型を持つ細胞と持たない細胞に分けることができ、その原因はダノン病の異常分子であるLAMP2が発現しているかしていないかであることを確認した。この２種類のiPS細胞が作成される理由がX染色体の不活性化の際によるものであることを、ヒトアンドロゲンレセプター抗体のCAGリピート数を用いて解明した。We successfully established two sets of induced pluripotent stem cell(iPSC) lines and iPSC derived cardiomyocyte(iPSC-CMs) subseqently, that expressed either wild-type or mutant LAMP2 allele from female with danon disease, of which only the populations expressing mutant LAMP2 showed autophagic failure. By means of androgen receptor methylation assays, we revealed that the XCI patterns of the iPSCs elucidate the LAMP2 expression and subsequent in vitro phenotype. The in vitro characteristics of generated iPSCs and iPSC-CMs did not rely on the clinical phenotype.研究課題/領域番号:16K19632, 研究期間(年度):2016-04-01 - 2019-03-31出典：研究課題「女性ダノン病iPS細胞由来心筋を用いたXISTの役割と遺伝子治療の可能性について」課題番号16K19632
（KAKEN：科学研究費助成事業データベース（国立情報学研究所））
（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-16K19632/16K19632seika/)を加工して作成research repor
Author-wise bibliometric analysis based on entropy.
No full textAuthor-wise bibliometric analysis based on entropy.</p
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