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Course Syllabus FSAD 2650-Fa10
No full textFSAD 2650, Patternmaking For Fashion Design, Course Syllabus by Instructor Anita Racine, Fall 201
Ms. 2650
Get PDFManuscrito número 2650 de la Biblioteca Histórica de la USAL1 h.g., X h., 58 f., 1 h., 1 h.g. : papel ; 223x130 mmLatín. Letra humanística cursivaEn h. IV: escudo de Martín de Córdoba y Mendoza, Obispo de Plasencia, en color. - En h. V: escudo de Plasencia en color. - Entre h. VI-VII: plano de Plasencia (285x400 mm) y en el vuelto, índice de lugares señalados en el plano. - Entre f. 51-52: esquema de la Diócesis (240x225 mm
The role of Galectin-3 and Survivin in invasion and drug resistance in human cancer
Get PDFGalectin-3 is an apoptosis-related gene previously found to be over-expressed in invasive tumours and to cause in v itroinvasiveness and metastasis in colon, breast and thyroid follicular cancer cells. Galectin-3 over-expressing clones were obtained following stable transfection of galectin-3 cDNA into the non-invasive human lung carcinoma cell line DLKP. These clones exhibited increased invitro invasiveness and motility and altered cell adhesion properties, but did not exhibit a drug resistant phenotype. Survivin is an antiapoptotic gene highly expressed in all cancer types and during fetal development, but not in most normal adult tissue. To determine whether survivin over-expression plays a role in drug-resistance, transient transfections of survivin cDN into SKOV-3 ‘Tet off , MCF-7 'Tet off and DLKP cells, were carried out. All three transfections resulted in an overexpression of survivin mRNA, but not survivin protein.
To further investigate the role o f galectin-3 and survivin in drug resistance and invasiveness, RT-PCR and western blot analysis were carried out on DLKP and RPMI- 2650 cells which had been exposed to sequential pulsing with three (vincristine, taxotere and 5-fluorouracil) and five (vincristine, 5-fluorouracil, CCNU, carboplatin and epirubicin) chemotherapy drugs, respectively. Drug selection of DLKP resulted in two MDR variants, DLKP-taxotere and DLKP-vincristine. The taxotere-resistant variant displayed a highly invasive phenotype. Drug selection of RPMI-2650 cells resulted in unstable MDR variants, with no induction of invasiveness. Galectin-3 expression was examined in the drug-selected variants, along with the expression of the MDR-related genes, by RT-PCR analysis. Galectin-3 mRNA expression was unaltered in all the drug-selected variants of DLKP and RPMI-2650. Survivin expression was examined in the drug-selected variants by RT-PCR and western blot analysis. Survivin protein expression was dramatically down-regulated in DLKP-vincristine and DLKP-taxotere MDR variants. This decrease was not observed at the mRNA level, indicating that this down-regulation of survivin protein may be at the post-transcriptional level in this cell system. In addition, DNA microarray technology was used to investigate differences in gene expression between the DLKP MDR variants and to identify new targets involved in MDR and invasion/metastasis. Results indicated a dramatic increase o f Mdr-1 expression and a slight increase of MRP2 expression in DLKP-taxotere and DLKP-vincristine. The expression of other genes studied were unaltered in the MDR variants compared to the parental cells.
Finally, to establish a role for apoptosis-related and drug resistance-related genes as potential clinical markers in breast cancer, the expression of survivin, galectin-3 and MRP1 genes were examined in breast tumour specimens, by RT-PCR analysis. The expression of these genes was correlated with clinicopathological parameters to investigate an association between them and gene expression. As galectin-3 was expressed in almost all the tumour specimens, it was not possible to correlate its expression with any of the parameters. No significant association was detected between disease outcome and the mRNA expression of either survivin or MRP1
Course Syllabus FSAD 2650-Fa 2012
No full textFSAD 2650, Patternmaking For Fashion Design, Course Syllabus by Instructor Anita Racine, Fall 201
In-cloud Measurements of Drop Dynamics
No full textWe here present the results of a measurement campaign at the Umweltforschungsstation Schneefernerhaus (UFS) research station at an elevation of 2650 m, on the mountain top of Zugspitze, Germany. We measured the three-dimensional motion of the water droplets inside a turbulent cloud using three high-speed stationary cameras. We show the statistics of droplet acceleration and velocities, and their dependence on the flow parameters
Block Card 2650 Elsie Avenue
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: Dwelling | 2650 Elsie Avenue (Toledo, Ohio) | Cape Cod Style | Owens Addition (Toledo, Ohio) | Deveaux Area (Toledo, Ohio) | West Toledo area (Toledo, Ohio
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Block Card 2650 Strauss Avenue
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: attached garage | Ranch Style | 2650 Strauss Avenue (Toledo, Ohio) | Dwelling | McKondin Heights Addition | Westgate Are
Block Card 2650 107th Street
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: Dwelling | Hipped roof | Ranch houses | Shore Acres (Toledo, Ohio) | Point Place (Toledo, Ohio) | North Toledo Area (Toledo, Ohio) | 2650 107th Street (Toledo, Ohio
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