1,721,281 research outputs found

    CIL:24818

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    Intracellular localization of GFP-Vps74 K178A, R181A mutant in BY4742 sac1∆ vps74∆ mutant. SAC1 encodes an integral membrane phosphoinositide phosphatase and in the sac1∆ vps74∆ mutant, GFP-Vps74 localizes, in addition to the Golgi apparatus, to nuclear ER and cortical ER and/or PM (CIL# 24816). This localization of GFP-Vps74 is lost when two amino acids are mutated in the sulfate-binding pocket. This study demonstrates that the sulfate-binding pocket of Vps74 and GOLPH3 mediates PtdIns4P-binding and is essential for function. Cells grown in liquid medium were mounted in growth medium and 3D image stacks were collected at 0.4-µm z increments on a DeltaVision workstation (Applied Precision) based on an inverted microscope (IX-70; Olympus) using a 100× NA 1.4 oil immersion lens. Images were captured at 23C with a 12-bit CCD camera (CoolSnap HQ; Photometrics) and deconvolved using the iterative-constrained algorithm (Agard, 1984) and the measured point spread function. One image from the approximate center of z stack is shown in Fig4A K178A R181A/sac1∆ panel in J Cell Biol. 187: 967-975. 2009. Images in Fig 4A include CIL#s 13449, 13451, 13453, 13455, 13456, 13457, 24816, 24817, 24818, 24819, 24820, 24821

    Ross, A D M, 24818

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    This record was harvested from a previous catalogue system and will be withdrawn in 2025. Information in this record may be superseded or incomplete. Visit this record in UMA's new catalogue at: https://archives.library.unimelb.edu.au/nodes/view/414332Surname: ROSS. Given Name(s) or Initials: A D M. Military Service Number or Last Known Location: 24818. Missing, Wounded and Prisoner of War Enquiry Card Index Number: 49315.233544 Item: [2016.0049.46593] "Ross, A D M, 24818

    Images: Passiflora sulcatasperma ETMNH 24818

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    Passiflora sulcatasperma Hermsen Photographs of ETMNH 24818, a seed fragment that is a paratype of Passiflora sulcatasperma from the Pliocene Gray Fossil Site of Tennessee. Specimen information Location: Gray Fossil Site, Washington County, Tennesssee, U.S.A. Age: early Pliocene Repository:  East Tennessee State University Museum of Natural History (ETMNH) collections at Gray Fossil Site, Gray, Tennessee, U.S.A.     Related publication Hermsen, E.J. 2023. Pliocene seeds of Passiflora subgenus Decaloba (Gray Fossil Site, Tennessee) and the impact of the fossil record on understanding the diversification and biogeography of Passiflora. American Journal of Botany [accepted manuscript]. https://doi.org/10.1002/ajb2.16137</p

    Author in Gesaku. Author's Gesaku

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    pdfThat "play" (ge) equals "writing" (saku) is the premise of gesaku, which is maintained as a stance not only by early bushi-class authors but also by later ones who were professional, and sometimes best-seller, authors. Writing is a game in which the reader is invited to participate, the work being an open arena for the play of writing and interpretation rather than a finished product to be read for cohesive meaning. Thus gesaku is characterized by word-play and self-referentiality. This lecture traces these two features in eighteenth-century British poetry and prose and in gesaku writings of the later Edo period, drawing examples from several works in which the author's self-portrait is prominent. In comical poetry, Alexander Pope, in his "An Epistle to Dr. Arbuthnot," sets up the poet himself as an ideal against which vices are to be attacked and by which the satirist is to be defended for his writing. Jonathan Swift's "On the death of Dr. Swift" portrays the poet as the worst embodiment of human hypocrisy thereby inducing the reader to laugh at himself while laughing at the author. In prose fiction, Laurence Sterne's Tristram Shandy is a "picture of himself," as he calls it, in the sense that the very act of narrating replaces any objective narrative arrangement. Play, not necessity, seems to be the mother of literary invention. Pope invents the mock-epic couplet in which the sublime and the vulgar are mercilessly mixed ; Swift, the plain-style conversation in which the true and dispicable self is exposed ; and Sterne, the free-form narrative in which tricks of sound and spelling open up multiple possibilities for interpretation. Similarly, Edo gesaku writers are creators of new language. Hiraga Gennai's sense of the self is a match with Pope's, and, indeed, his Hohiron Part II is as much a "bill of complaint" as Pope's "Arbuthnot " Gennai's self-image, however, is double-sided : it is as proud as Pope's while being as self-debasing as Swift's, so that he simultaneously achieves the former's satirical dimension and the latter's depth. His mock-Chinese (kanbun-yomikudashi) style is more complex and open to interpretation than Pope's mockheroic couplet. The ludicrously lovable nose which Santô Kyôden assigns to his own face in his sketches indicates his inclination toward commercial popularity. His "yellow cover "(kibyôshi) books play on the reading of pictures as well as of the written texts. Particularly, his Kiji Nakazawa features the reader rather than the author so that reading constitutes narration, thus challenging Tristram Shandy for the title of the world's strangest narration. His Sakusha Tainai Totsuki no Zu, showing the formation of a book from its conception to publication as an embryo growing in the author’s masculine “womb,” epitomizes the late gesaku of bringing forward the backstage of writing. Many of Jippensha Ikku’s works are of this type but lacking Kyôden’s fictionality. In his Naruhodo Nekkara Ikku ga Saku, a slapstick comedy about the struggle of the author in search of ideas, Ikku, the popular author, depends on the reader’s indulgence in laughing at, and sympathizing with, the misery of the actual author. Here the author-reader relationship is extremely close, to such a degree to invalidate the written work, a case of a game losing its raison d’être by the conspiracy of the players.conference pape

    Going Beyond Counting First Authors in Author Co-citation Analysis

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    The present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed

    Variations on the Author

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    “Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship

    Appropriate Similarity Measures for Author Cocitation Analysis

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    We provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis

    Dispelling the Myths Behind First-author Citation Counts

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    We conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more sophisticated methods

    Author Index

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