1,744,297 research outputs found
Comparison of quantitative real time PCR with Sequencing and ribosomal RNA-FISH for the identification of fungi in Formalin fixed, paraffin-embedded tissue specimens
No full textBackground: Identification of the causative agents of invasive fungal infections (IFI) is critical for guiding antifungal therapy. Cultures remain negative in a substantial number of IFI cases. Accordingly, species identification from formalin fixed, paraffin embedded (FFPE) tissue specimens by molecular methods such as fluorescence in situ hybridisation (FISH) and PCR provides an appealing approach to improve management of patients. Methods: We designed FISH probes targeting the 28S rRNA of Aspergillus and Candida and evaluated them with type strains. Fluorescence microscopy (FM), using FISH probes and quantitative broadrange fungal PCR targeting the rRNA gene were applied to FFPE tissue specimens from patients with proven IFI in order to explore benefits and limitations of each approach. Results: PCR followed by sequencing identified a broad spectrum of pathogenic fungi in 28 of 40 evaluable samples (70%). Hybridisation of FISH probes to fungal rRNA was documented in 19 of 40 tissue samples (47.5%), including 3 PCR negative samples with low fungal burden. The use of FISH was highly sensitive in invasive yeast infections, but less sensitive for moulds. In samples with hyphal elements, the evaluation of hybridisation was impaired due to autofluorescence of hyphae and necrotic tissue background. Conclusions: While PCR appears to be more sensitive in identifying the causative agents of IFI, some PCR negative and FISH positive samples suggest that FISH has some potential in the rapid identification of fungi from FFPE tissue samples
Diptera Collectionis P. Gabriel Strobl - IV (Exemplare-Nr. 1891-2334).
No full textIm IV. Teil der Mitteilungen über das bearbeitete Dipterenmaterial der Kollektion Strobl werden die Exemplare 1891 bis 2334 (nur Acalyptrata) erfaßt.The fourth part of the evaluated material from Strobl’s collection of Diptera covers the specimens 1891-2334 (Acalyptrata only)
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Block Card 2334 Roseview Drive
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: Cottage Style | 2334 Roseview Drive (Toledo, Ohio) | Dwelling | Ferndale Addition (Toledo, Ohio) | Trilby Area (Toledo, Ohio)
B.N. fr 2334
No full textFiche en cours d'élaboration Dernière mise à jour : 2 août 2024 Ms. B.N. fr 2334. Datation : 1ère moitié du XVIe siècle, selon Richard Cooper. Ancienne cote : Anc. 8043. Il s'agit d'un recueil intégralement poétique, difficilement déchiffrable, d'une même main (qui est aussi celle qui copie le recueil de poésie française constituant la seconde partie du ms. BN fr 2336), à part pour les annotations marginales. Plan de la fiche 1. Organisation du manuscrit 2. Feuillets intercalés et annotatio..
Block Card 2334 Middlesex Drive
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: English Cottage Style | 2334 Middlesex Drive (Toledo, Ohio) | Dwelling | Old Orchard Addition (Toledo, Ohio) | Old Orchard Area (Toledo, Ohio
Block Card 2334 Pemberton Drive
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: Modern Colonial Style | 2334 Pemberton Drive (Toledo, Ohio) | Dwelling | Old Orchard Addition (Toledo, Ohio) | Old Orchard Area (Toledo, Ohio
Block Card 2334 Densmore Drive
No full textThis image was produced by the Auditor's Office in Lucas County, Ohio for tax assessment purposes. Associated dates are approximate. Descriptive terms related to this photograph include: English Cottage Style | 2334 Densmore Drive (Toledo, Ohio) | Dwelling | Old Orchard Addition (Toledo, Ohio) | Old Orchard Area (Toledo, Ohio
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