1,722,346 research outputs found
<i>S</i>. Typhimurium strain 14028 is pathogenic to <i>C. elegans</i>.
No full text<p>(A) Survival of wild-type N2 nematodes was compared when fed <i>E. coli</i> OP50 or <i>S</i>. Typhimurium 14028 (<i>p</i><0.0001). (B) Survival of nematodes was compared when infected with non-fluorescent <i>S</i>. Typhimurium 14028, 14028::GFP (<i>p</i> = 0.2251) or 14028::RFP (<i>p</i> = 0.9384; all <i>p</i> values as compared to the non-fluorescent strain). Each survival curve is representative of 3 independent assays, each with 3 plates per strain and 20 nematodes per plate.</p
14028<sup>hyd3</sup> is impaired in early gut ecosystem invasion.
No full text<p>Gut colonization was monitored in two different mouse models: streptomycin-pretreated conventional mice (sm-SPF) and low complexity microbiota (LCM) mice. Mice were infected with a 1∶1 mixture (5×10<sup>7</sup> cfu by gavage) of 14028<sup>hyd3</sup> and the isogenic background strain (14028<sup>WT</sup>). Fecal loads of both strains were determined by selective plating. (A) Competitive infection indices were determined over 4 days. Ns = not significant (P≥0.05), ** P<0.01, Mann-Whitney U test. (B) Bacterial loads of both competing strains (14028<sup>WT</sup> and 14028<sup>hyd3</sup>) are depicted Ns = not significant (P≥0.05), * P<0.05; one-tailed Wilcoxon matched pairs signed rank test on paired data (dashed lines).</p
Efecto antibacteriano in vitro del extracto etanólico de Moringa oleífera (moringa) frente a cepas de Escherichia coli atcc 25922 y Salmonella Typhi atcc 14028
No full textMoringa Oleifera (moringa), planta con propiedades benéficas, por ejemplo, su capacidad antibacteriana ante algunos patógenos como Escherichia coli, Pseudomonas, Salmonellas, Staphylococcus, bacillus cereus y Streptococcus. El objeto de la investigación fue la evaluación del efecto antibacteriano in vitro del extracto etanólico de Moringa Oleífera (moringa) en cepas de Escherichia coli ATCC 25922 y Salmonella typhi ATCC 14028, con diseño experimental, enfoque cuantitativo transversal y muestreo simple, muestras de E. coli y Salmonella, concentraciones de extracto de moringa: 100, 75 y 50 mg/ml, por 4 repeticiones cada una. Los resultados principales fueron relacionados con el diámetro de halos, evidenciando que el efecto antibacteriano en las dos cepas es directamente proporcional frente a concentraciones de extracto, los promedios de halos inhibitorios según HSD Tukey y de acuerdo con el grado de sensibilidad fueron de 17.5 mm, 13.0 mm y 6.83 mm para E. coli, para Salmonella 15.6 mm, 11.5 mm y 7.14 mm, concluyéndose que es de carácter significativo y directamente proporcional el efecto antibacteriano del extracto etanólico de Moringa oleífera en cepas de Escherichia coli ATCC 25922 y Salmonella Typhi ATCC 14028, con rangos de halos desde 5 mm a 18 y 5mm a 16 mm respectivamente, mostrándose una eficacia antibacteriana
Genotype and characteristics of <i>S</i>. Typhimurium ATCC 14028 mutant strains used in this study.
No full textGenotype and characteristics of S. Typhimurium ATCC 14028 mutant strains used in this study.</p
Going Beyond Counting First Authors in Author Co-citation Analysis
No full textThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Detection of Salmonella typhimurium ATCC 14028 in Powder Prepared Traditional Medicines Using Real-Time PCR
No full textThe detection of Salmonella typhimurium ATCC 14028 using real-time PCR on powdered traditional medicinal products was carried out in the microbiology and molecular biology testing laboratory of the Food and Drug Administration in Gorontalo. This research aims to provide a reference for alternative testing methods in testing the products of traditional powder preparations on the market. The sample consisted of 10 traditional powder preparations spiked with positive control of S. typhimurium ATCC 14028 phase 2. The method used in the study was real-time PCR analysis using the SYBR® Green method, while DNA isolation using the direct PCR method. Data analysis was performed by analyzing the sample's melting temperature (Tm) curve and comparing it with positive control. The results showed that S. typhimurium ATCC 14028 was detected in samples at an average Tm value of 84.18°C, with ranges of 84.0-84.5°C. For positive control, the Tm value was at 85.2°C, while for the negative control, the Tm value was not detected. Based on these data, it can be concluded that S. typhimurium ATCC 14028 in traditional medicine products powder preparations can be detected using real-time PCR
Variations on the Author
No full text“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
No full textWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
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