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Circular_0086414 induces SPARC like 1 (<i>SPARCL1</i>) production to inhibit esophageal cancer cell proliferation, invasion and glycolysis and induce cell apoptosis by sponging miR-1290
No full textCircular RNA (circRNA) plays an important role in cancer progression. Here, we investigated the function of circ_0086414 in the malignant progression of esophageal cancer (EC). RNA expression of circ_0086414, microRNA-1290 (miR-1290), and SPARC like 1 (SPARCL1) was detected by quantitative real-time polymerase chain reaction. The protein levels of N-cadherin, E-cadherin, and SPARCL1 were checked by Western blotting analysis. Cell proliferation was investigated by 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT), 5-Ethynyl-29-deoxyuridine (EdU), and cell colony formation assays. Cell invasion and apoptosis were analyzed by transwell invasion assay and flow cytometry analysis, respectively. Glycolysis was evaluated by analyzing glucose consumption and lactate production. In an xenograft mouse model, the effect of circ_0086414 on tumor tumorigenesis was investigated. The interactions among circ_0086414, miR-1290, and SPARCL1 were identified by dual-luciferase reporter and RNA pull-down assays. Results showed that circ_0086414 and SPARCL1 expression were significantly downregulated, while miR-1290 was upregulated in EC tissues and cells. EC patients with low circ_0086414 expression had a poor prognosis. Increasing circ_0086414 expression led to decreased EC cell proliferation, invasion and glycolysis and increased cell apoptosis, accompanied by a decrease of N-cadherin expression and an increase of E-cadherin expression. Also, the enforced expression of circ_0086414 delayed tumor tumorigenesis. Besides, circ_0086414 acted as a miR-1290 sponge and regulated EC cell processes by binding to the miRNA. MiR-1290 also participated in EC malignant progression through SPARCL1. Further, circ_0086414 stimulated SPARCL1 production by negatively regulating miR-1290. Thus, circ_0086414 inhibited EC cell malignancy through the miR-1290/SPARCL1 pathway, providing a reliable target for the therapy of EC.</p
Additional file 2 of Exo-miR-1290-induced by COX-2 overexpression promotes cancer-associated fibroblasts activation and tumor progression by CUL3-Nrf2 pathway in lung adenocarcinoma
No full textAdditional file 2. The effects of COX-2 inhibitor on the miR-1290 expression and CAFs activation
Company 1290
No full textGroup photo in front of a tent at the CCC camp. Most men are in uniform but two enrollees on each end are wearing white t-shirts. Writing over the photo reads: ""Company 1290 CCC Idaho 1933"".P-212C-129
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Expression level of miR-1290 target genes.
No full text<p>A) Changes in expression level of <i>MAF</i>, <i>ITPR2</i> and <i>KIF13B</i> genes in UT-SCC-34 treated by miR-1290 inhibitor (black graph) compared to both UT-SCC-34 treated by negative control (dark gray graph) as well as UT-SCC-34 wild type (gray graph). * fold change in UT-SCC-34 treated by miR-1290 inhibitor compared to UT-SCC-34 treated by negative control. B) Changes in expression level of MAF protein in UT-SCC-34 and UT-SCC-107cell lines treated by miR-1290 inhibitor (black graph) compared to both cells treated by negative control (dark gray graph) as well as wild type cells (gray graph). * fold change in cells treated by miR-1290 inhibitor compared to cells treated by negative control.</p
217. Asahara Tameyori (?-1290)
No full textIwao Seiichi, Sakamato Tarō, Hōgetsu Keigo, Yoshikawa Itsuji, Kobayashi Tadashi, Bonmarchand Georges, Kanazawa Shizue. 217. Asahara Tameyori (?-1290). In: Dictionnaire historique du Japon, volume 1, 1963. Lettre A. pp. 64-65
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
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