1,738,794 research outputs found
Up-regulation of miR-1266-5p suppressed hTERT expression and telomerase activity in cancer cell lines
Get PDFBackground: Telomerase is in charge of telomere extending and is triggered in around 90% of cancers. hTERT is the controlling subunit of telomerase and plays a critical role in the activation of telomerase. The mechanism through which hTERT regulate the invasion and metastasis of cancer is unclear. miRNAs can regulate the expression of hTERT. It was previously reported that miR-1266 can target hTERT in gastric cancer. In this study, we have made the first report of miR-1266-5p role on the hTERT expression, telomerase activity, and biological functions, including cell proliferation and cell cycle in AGS, MCF7, A375, and HepG2 cell lines.
Methods: AGS, MCF7, A375, and HepG2 cell lines were transfected with miR-1266-5p mimic and inhibitor reagents. The Expression levels of miR-1266-5p, hTERT, and transfection efficiency were analyzed by Taqman qRT-PCR. The cell proliferation and cell cycle changes were detected by MTT Calorimetric Assay and flow cytometry, respectively. Also, Quantitative TRAP Assay was used to detect telomerase activity.
Results: The expression of miR-1266-5p significantly was increased after transfection by mimic compared to control cells. While its expression was decreased by the inhibitor. Upregulated miR-1266-5p significantly decreased cell growth, although inhibitor promoted cell proliferation. This finding was confirmed by cell cycle analysis, as upregulation of miR-1266-5p induced cells cycle arrest at the transition of G1 to S phase and led to G0/G1 entry, while the downregulation of miR-1266-5p promoted cell growth and led to G2/M entry. Concordantly, the overexpression of miR-1266-5p resulted in down-regulated hTERT expression and also suppressed telomerase activity.
Conclusions: Taken together, the findings showed that miR-1266-5p acts as hTERT and telomerase activity suppressor. miR-1266-5p could also decrease cell proliferation and induce cell cycle arrest, while its inhibitor eliminates miR-1266-5p effects. Thus, upregulation of miR-1266-5p may be considered as a novel therapeutic target in cancer
Santa Fe (ATSF) 1266
No full textA photograph print showing Santa Fe (ATSF) 1266, 4-6-2 (BLW), class 1226, on passenger train, Dallas TX
Carpenter's Local Union #1266
No full textPhotograph of exterior of Carpenter's Local #1266 Hall, built in 1949. This building is located at 400 Josephine Street
J. Hartmann & Syn/Sohn, Prague VIII, Telefon 1266
No full textJ. HARTMANN & SYN/SOHN, PRAGUE VIII, TELEFON 1266
J. Hartmann & Syn/Sohn, Prague VIII, Telefon 1266 ( -
Meta Data Catalogue for LM22-Repository of the LandMan Information System in CRC 1266 (SFB 1266)
No full text<p>Overview of the meta data categories and predefined values for certain categories (see meta data description for when values are a basic subset or the space to choose from) used in the LM22-Repository component of the LandMan information system in CRC 1266 (SFB 1266). This catalogue is not necessarily final but organically growing / changing alongside continuous data collection activity due to newly (and repeatedly) occurring requirements not yet covered.</p>
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Cobb, Rebecca (SC 1266)
Get PDFFinding aid only for Manuscripts Small Collection 1266. Paper, “Thistle Cottage,” written by Rebecca Cobb for a WKU restoration class, documenting the history of this 1912 house built in Greenville, Kentucky for coal magnate William Graham Duncan
Analisis Yuridis terhadap Pengesampingan Pasal 1266 KUH Perdata dalam Kasus Pengakhiran Suatu Perjanjian Karena Terjadinya Wanprestasi
Get PDFArticle 1266 of the Civil Code explains that default by one party does not immediately terminate the agreement, but must first be brought before a judge. However, in its implementation, the parties usually do not pay attention to the provisions of Article 1266 of the Civil Code, so that if a default occurs, the agreement can be terminated unilaterally. The core problem in this article relates to the views of legal experts on the neglect of Article 1266 of the Civil Code and the application of the waiver clause of Article 1266 of the Civil Code based on court decisions. This research is a normative legal study that utilizes secondary data obtained from literature analysis. This article explains that there are differences of opinion among legal experts regarding whether the parties are allowed to ignore Article 1266 of the Civil Code, seen from the characteristics of the legal norms, the situations, and the parties involved in the agreement. Based on the analysis of court decisions by the Author, most judges agree with the application of the waiver clause of Article 1266 of the Civil Code based on Article 1338 paragraph (1) of the Civil Code. Thus, the agreement between the parties regarding the waiver of Article 1266 of the Civil Code in the agreement must be complied with by the parties concerned. Judges who reject the application of the waiver clause of Article 1266 of the Civil Code assume that the action is contrary to the provisions contained in Article 1266 of the Civil Code.Di Pasal 1266 Kitab Undang-Undang Hukum Perdata dijelaskan bahwa wanprestasi dari salah satu pihak tidak langsung mengakhiri perjanjian, tetapi harus terlebih dahulu dibawa ke hadapan hakim. Namun, dalam pelaksanaannya, pihak-pihak biasanya tidak memperhatikan ketentuan Pasal 1266 KUH Perdata, sehingga jika terjadi wanprestasi, perjanjian bisa diakhiri secara sepihak. Inti masalah dalam artikel ini berkaitan dengan pandangan para ahli hukum tentang pengabaian Pasal 1266 KUH Perdata serta penerapan klausul pengabaian Pasal 1266 KUH Perdata berdasarkan keputusan-keputusan pengadilan. Penelitian ini adalah studi hukum normatif yang memanfaatkan data sekunder yang diperoleh dari analisis literatur. Artikel ini menjelaskan bahwa terdapat perbedaan pandangan di antara ahli hukum mengenai apakah para pihak diperbolehkan untuk mengabaikan Pasal 1266 KUH Perdata, dilihat dari karakteristik norma hukumnya, situasi per situasi, serta pihak-pihak yang terlibat dalam perjanjian. Berdasarkan analisis putusan-putusan pengadilan oleh Penulis, sebagian besar hakim menyetujui penerapan klausul pengabaian Pasal 1266 KUH Perdata dengan dasar Pasal 1338 ayat (1) KUH Perdata. Dengan demikian, kesepakatan antara para pihak mengenai pengesampingan Pasal 1266 KUH Perdata dalam perjanjian wajib dipatuhi oleh pihak-pihak yang bersangkutan. Hakim yang menolak penerapan klausul pengabaian Pasal 1266 KUH Perdata beranggapan bahwa tindakan itu bertentangan dengan ketentuan yang terdapat dalam Pasal 1266 KUH Perdata tersebu
UGARSKI DOMINIKANAC IVAN DE BUZAD – BISKUP SKRADINSKI (1250. – 1266.) I NADBISKUP SPLITSKI (1266. – 1293./94.)
Get PDFZauzevši na temelju povijesnih izvora stav da je skradinski biskup Ivan Ugrin istovjetan splitskom nadbiskupu Ivanu de Buzadu, autor kritički obrađuje njegov lik i djelovanje. Nakon osvrta na historiografiju i izvore o ovom nadbiskupu, govori se o njegovu porijeklu i redovničkoj pripadnosti, o biskupskoj službi u Skradinu (1250. – 1266.) te nadbiskupskoj i metropolitskoj službi u Splitu (1266. – 1293./94.). Opisuje se njegova uloga u crkvenom i društvenom životu Splita, uloga u sukobima između Trogirske biskupije i Šibenika te odnos prema sufraganskim biskupijama. Na kraju rada govori se o matropolitanskoj sinodi koju je održao 1292. i okvirno se određuje vrijeme njegove smrti
- …
