1,720,961 research outputs found
Regulations of hematopoietic stem cell system by the control of intracellular ATP
Get PDF多くの組織幹細胞が静止期に存在する状況において、細胞内ATP濃度の調節は幹細胞の寿命や振る舞いを制御する上で重要な因子として考えられる。本研究課題においてはエネルギーセンサーとして機能するLKB1-AMPKシグナルに着目し、LKB1およびAMPK欠損造血幹細胞について解析を行った。その結果、これらの分子は造血幹細胞の維持に寄与していることを明らかにした。Many tissue specific stem cells stay dormant. Therefore, the regulation of intracellular ATP is important for the control of the longevity or behaviors of stem cells. The LKB1-AMPK signaling functions as an intracellular energy sensor. To examine the role of LKB1 or AMPK in the regulatory system of hematopoietic stem cell, I analyzed LKB1- or AMPK-deficient hematopoietic stem cells. Our data demonstrated that LKB1 and AMPK contributed to the maintenance of hematopoietic stem cells.研究課題/領域番号:23791076, 研究期間(年度):2011-2012出典：研究課題「細胞内ATP調節による造血幹細胞制御機構の解明」課題番号23791076
（KAKEN：科学研究費助成事業データベース（国立情報学研究所））
（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-23791076/23791076seika/）を加工して作成research repor
Analysis of hematopoietic stem cell and leukemic stem cell niche by focusing on Spred-1 functions
Get PDF本研究課題では、造血幹細胞と白血病幹細胞およびそれらのニッチについての相違点を理解することを目的としている。そのためにSpred-1ノックアウト造血幹細胞および白血病発症モデルマウスを用いて競合的骨髄移植法による解析を行なった。その結果、造血幹細胞と白血病幹細胞はよく似た表面マーカー・多分化能を示し、また一時的にニッチにおいて競合していることが示唆された。しかし、有意差を持って白血病発症マウスをレスキューすることはできなかった。The aim of this research project is to understand both the similarities and differences between hematopoietic stem cells and leukemic stem cells and their niches. I have carried out competitive repopulation assay using Spred-1 knockout and leukemia-developed mouse bone marrow cells. Leukemic stem cells showed cell surface marker phenotype and multipotency similar to that of hematopoietic stem cells. Furthermore, competitive repopulation assay with Spred-1 knockout bone marrow cells suggested that both stem cells might compete in their sharing niches. However, Spred-1 knockout cells could not significantly rescue the leukemia-developed mice.出典：研究課題「Spred-1の機能に着目した造血幹細胞及び白血病幹細胞ニッチの解析」課題番号21790910
（KAKEN：科学研究費助成事業データベース（国立情報学研究所））
（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-21790910/21790910seika/）を加工して作成金沢大学がん進展制御研究所research repor
Going Beyond Counting First Authors in Author Co-citation Analysis
Get PDFThe present study examines one of the fundamental aspects of author co-citation analysis (ACA) - the way co-citation
counts are defined. Co-citation counting provides the data on which all subsequent statistical analyses and mappings
are based, and we compare ACA results based on two different types of co-citation counting - the traditional type that
only counts the first one among a cited work's authors on the one hand and a non-traditional type that takes into
account the first 5 authors of a cited work on the other hand. Results indicate that the picture produced through this non-traditional author co-citation counting contains more coherent author groups and is therefore considerably clearer. However, this picture represents fewer specialties in the research field being studied than that produced through the traditional first-author co-citation counting when the same number of top-ranked authors is selected and analyzed. Reasons for these effects are discussed
Regulation of hematopoietic stem cell function by calorie restriction
Get PDF近年、造血幹細胞を含む複数の組織幹細胞において、個体レベルでのカロリー摂取量がその動態に大きな影響を及ぼすことが判明し、新たな幹細胞制御機構として注目されている。本研究課題では造血幹細胞をモデル系として、カロリー摂取制限がいかに幹細胞の自己複製や分化に影響を与えるのか、またそれが幹細胞に直接作用しているのか或いはニッチ細胞を介しているのかを明らかにすることを目的として解析を行なった。その結果、カロリー摂取制限は表現型的には造血幹細胞の数を増やす傾向にあったが、機能的には大きな影響を与えないことが明らかとなった。Recent studies have indicated that calorie restriction affects the regulation of tissue specific stem cells. Thus, calorie control has been thought as a novel regulatory machinery of stem cell regulation. In this study, I have investigated how calorie restriction influences regulation of hematopoietic stem cell system. I found that calorie restriction phenotypically increased the number of hematopoietic stem cells, while there is no advantage in stem cell function.研究課題/領域番号:25461411, 研究期間(年度):2013-04-01 - 2016-03-31出典：研究課題「カロリー制限による造血幹細胞動態制御の分子基盤の解析」課題番号25461411
（KAKEN：科学研究費助成事業データベース（国立情報学研究所））
（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-25461411/25461411seika/）を加工して作成金沢大学がん進展制御研究所research repor
Study of the regulatory mechanisms of hematopoietic stem cell homeostasis mediated by alterations in the nutritional environment
Get PDFAn excessively unbalanced diet is one of the causes of obesity and lifestyle diseases. In this study, we aim to elucidate the regulatory mechanisms of hematopoietic stem cell (HSC) homeostasis mediated by gut microbiota-derived metabolites, which are altered by dietary habits. We have found that a gut microbiota-derived metabolite induced by a high-fat diet feeding suppresses the HSC functions. Furthermore, we have shown that a gut microbiota-derived metabolite induced by high-dietary fiber diet feeding enhances the HSC functions. Our findings may contribute to development of prevention and treatment improvement of leukemogenesis and lifestyle diseases caused by diet-induced hematopoietic abnormalities.本研究では食餌によって変化する腸内細菌由来代謝産物による造血幹細胞の恒常性維持機構について解明することを目的として、高脂肪食および繊維食により変動する腸内細菌由来代謝物の探索と造血幹細胞への影響の解析を行った。その結果、高脂肪食で変動する代謝物に造血幹細胞の機能抑制に作用するものが存在することを見出した。さらに、高繊維食による腸内細菌の変化で変動する代謝物の中に、造血幹細胞の未分化性維持に作用するものが存在することを明らかにした。本研究により、食餌により変化する腸内細菌由来代謝産物による造血幹細胞の恒常性維持機構の解明を進めた。研究課題/領域番号:19K08860, 研究期間(年度):2019-04-01 - 2022-03-31出典：研究課題「栄養環境変化による造血幹細胞恒常性維持機構の解明」課題番号19K08860
（KAKEN：科学研究費助成事業データベース（国立情報学研究所））
（https://kaken.nii.ac.jp/ja/report/KAKENHI-PROJECT-19K08860/19K08860seika/）を加工して作成金沢大学がん進展制御研究所research repor
Analysis of functions of Spred-1 in melanocyte stem cells and common molecular mechanisms in tissue-specific stem cell regulations
Get PDF本研究課題では、様々な組織幹細胞システムの制御機構における共通分子基盤の解明を目的とし、Spred-1に着目して造血幹細胞と色素幹細胞システムを中心に解析を行なった。Spred-1欠損マウスの解析から、血液系ではSpred-1はサイトカインである幹細胞因子(SCF)による刺激を抑制的に制御することによって造血幹細胞とその維持・分化の場所であるニッチとの親和性を調節していることが示唆された。一方色素系でも同様にSpred-1欠損マウスを用いた解析を行なった結果、色素細胞が過剰に増殖していることを明らかにした。研究課題/領域番号:19790775, 研究期間(年度):2007-2008出典：「色素幹細胞におけるSpred-1の機能と組織幹細胞制御機構の共通分子基盤の解明」研究成果報告書 課題番号19790775
（KAKEN：科学研究費助成事業データベース（国立情報学研究所））
（https://kaken.nii.ac.jp/report/KAKENHI-PROJECT-19790775/19790775seika/)を加工して作成research repor
Variations on the Author
Get PDF“Variations on the Author” discusses two of Eduardo Coutinho’s recent films (Um Dia na Vida, from 2010, and Últimas Conversas, posthumously released in 2015) and their contribution to the general question of documentary authorship. The director’s filmography is characterized by a consistent yet self-effacing form of authorial self-inscription: Coutinho often features as an interviewer that rather than express opinions propels discourses; an interviewer that is good at listening. This mode of self-inscription characterizes him as an author who is not expressive but who is nonetheless markedly present on the screen. In Um Dia na Vida, however, Coutinho is completely absent form the image, while Últimas Conversas, on the contrary, includes a confessional prologue that moves the director from the margins to the center of his films. This article examines the ways in which these works stand out in the filmography of a director who offers new insights into the notion of cinematic authorship
Appropriate Similarity Measures for Author Cocitation Analysis
Get PDFWe provide a number of new insights into the methodological discussion about author cocitation analysis. We first argue that the use of the Pearson correlation for measuring the similarity between authors’ cocitation profiles is not very satisfactory. We then discuss what kind of similarity measures may be used as an alternative to the Pearson correlation. We consider three similarity measures in particular. One is the well-known cosine. The other two similarity measures have not been used before in the bibliometric literature. Finally, we show by means of an example that our findings have a high practical relevance.information science;Pearson correlation;cosine;similarity measure;author cocitation analysis
Dispelling the Myths Behind First-author Citation Counts
Get PDFWe conducted a full-scale evaluative citation analysis study of scholars in the XML research field to explore just how different from each other author rankings resulting from different citation counting methods actually are, and to demonstrate the capability of emerging data and tools on the Web in supporting more realistic citation counting methods. Our results contest some common arguments for the continued
use of first-author citation counts in the evaluation of scholars, such as high correlations between author rankings by first-author citation counts and other citation
counting methods, and high costs of using more realistic citation counting methods that are not well-supported by the ISI databases. It is argued that increasingly available digital full text research papers make it possible for citation analysis studies to go beyond what the ISI databases have directly supported and to employ more
sophisticated methods
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