1,721,434 research outputs found
NOTCH SIGNALLING IN VASCULAR SMOOTH MUSCLE CELL SURVIVAL
Get PDFVascular smooth muscle cells (VSMCs), in the fibrous cap of atherosclerotic plaques, play an essential role in maintaining plaque stability. Apoptosis of VSMCs leads to plaque rupture resulting in myocardial infarction and sudden death. A number of signalling pathways contribute to the regulation of VSMC growth and survival, and Notch signalling is a critical pathway in this process. We have previously reported the anti-apoptotic role of Notch signalling by stable transfection of Notch3 in Human Embryonic Kidney (HEK) 293 cells. VSMCs were used to explore the molecular mechanisms of Notch signalling in VSMC survival. Endogenous Notch signalling was stimulated in vitro by immobilised Notch ligand Jagged1/FC chimera in a WKY rat smooth muscle cell line; exogenous Notch signalling was introduced by stable transfection of Notch3 or the constitutive active form of Notch3 (N3IC) in these cells. Western blotting of cells following either Jagged1 stimulation or overexpression of Notch3 or N3IC showed up-regulations of total Akt and phosphorilated-Akt, a key component of the cell survival pathway. The activation of Akt, induced by Jagged1, is associated with decreased expression of PTEN, a negative regulator of the Akt pathway. Activation of the Akt pathway contributes, at least in part, to Notch signalling-mediated VSMC survival. A possible mechanism is via suppression of PTEN expression. Further studies aim at elucidating the signalling network that relates to VSMC survival by the role of Notch receptors
NOTCH SIGNALLING IN VASCULAR SMOOTH MUSCLE CELL SURVIVAL
Get PDFVascular smooth muscle cells (VSMCs), in the fibrous cap of atherosclerotic plaques, play an essential role in maintaining plaque stability. Apoptosis of VSMCs leads to plaque rupture resulting in myocardial infarction and sudden death. A number of signalling pathways contribute to the regulation of VSMC growth and survival, and Notch signalling is a critical pathway in this process. We have previously reported the anti-apoptotic role of Notch signalling by stable transfection of Notch3 in Human Embryonic Kidney (HEK) 293 cells. VSMCs were used to explore the molecular mechanisms of Notch signalling in VSMC survival. Endogenous Notch signalling was stimulated in vitro by immobilised Notch ligand Jagged1/FC chimera in a WKY rat smooth muscle cell line; exogenous Notch signalling was introduced by stable transfection of Notch3 or the constitutive active form of Notch3 (N3IC) in these cells. Western blotting of cells following either Jagged1 stimulation or overexpression of Notch3 or N3IC showed up-regulations of total Akt and phosphorilated-Akt, a key component of the cell survival pathway. The activation of Akt, induced by Jagged1, is associated with decreased expression of PTEN, a negative regulator of the Akt pathway. Activation of the Akt pathway contributes, at least in part, to Notch signalling-mediated VSMC survival. A possible mechanism is via suppression of PTEN expression. Further studies aim at elucidating the signalling network that relates to VSMC survival by the role of Notch receptors
Professor Mohammad Sadli - Conference "Indonesia's New Order"
No full textProfessor Mohammad Sadli, Indonesia's former Minister for Mines and Energy and Professor of Economics at the University of Indonesia, was Keynote speaker at the conference.
ANU Reporter - Vol.20, No.20 (8 December 1989
Berbeda tetapi Setara : Pemikiran tentang Kajian Perempuan
No full textMelalui buku ini Saparinah Sadli ingin memaknai perjalanan perempuan Indonesia. Banyak tulisan-tulisan Saparinah lainnya dalam rentang waktu 1984-1999 yang bercerita tentang perempuan Indonesia. Buku berjudul Berbeda tetapi Setara, Pemikiran tentang Kajian Perempuan memperlihatkan perubahan wanita Indonesia dari waktu ke waktu yang membahas tentang pemahaman, masalah dan kebutuhan perempuan Indonesia di masyarakat serta perlindungan, penghargaan juga pemenuhan haknya oleh negara.Dalam buku ini jelas terasa adanya sebuah optimisme perubahan yang membawa banyak kemajuan bagi perempuan Indonesia dan juga mengingatkan kita sebagai perempuan untuk terus menjaga perubahan tersebut agar tidak berbalik membelenggu kaum wanita
Mutu pelayanan keluarga berencana di Indonesia
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The quality of FP service in Indonesia still shows some weak points i.e. weaknesses in the quality of giving information and in that of interpersonal interaction, so that the clients who want to optimally get their satisfaction in the choice of effective, economically reachable, safe, and suitable methods become vague. Saparinah Sadli, in her writing shows the weak points of the FP service based on an emperical study. Further, she also shows the solution formula related to the Cairo action program
KeywordsFamily Plannin
MUTU PELAYANAN KELUARGA BERENCANA DI INDONESIA
Get PDFThe quality of TP service in Indonesia stillshows some weak points i.e. weaknesses in the quality ofgimng information and in that of interpersonal interaction, so that the clients who want to optimally get their satisfaction in the choice of effective, economically reachable, safe, and suitable methods become vague. Saparinah Sadli, in her writing shows the weak points of the TP service based on an emperical study. Turther, she also shows the solution formula related to the Cairo action program
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