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Doña Mencía la Adelantada: una expedición al paraíso
No full textSilenciada» por la historiografía oficial durante siglos, la vida de Doña Mencía Calderón de Sanabria, viuda del Tercer Adelantado del Río de la Plata, ha sido rescatada del olvido gracias a la publicación de algunas novelas. Con el paso del tiempo, su figura, al principio glorificada como heroína de una empresa excepcional (cruzar en 1550 el Atlántico al mando de un pequeño «ejército» de mujeres destinadas a crear un hogar en Nuestra Señora de la Asunción) se ha transformado en un hito legendario de modernidad
The Protein Disulfide Isomerase gene family in bread wheat (T. aestivum L.)
No full textL'articolo é disponibile sul sito dell'editore: http://www.biomedcentral.comBackground: The Protein Disulfide Isomerase (PDI) gene family encodes several PDI and PDI-like proteins containing
thioredoxin domains and controlling diversified metabolic functions, including disulfide bond formation and
isomerisation during protein folding. Genomic, cDNA and promoter sequences of the three homoeologous wheat
genes encoding the "typical" PDI had been cloned and characterized in a previous work. The purpose of present
research was the cloning and characterization of the complete set of genes encoding PDI and PDI like proteins in bread
wheat (Triticum aestivum cv Chinese Spring) and the comparison of their sequence, structure and expression with
homologous genes from other plant species.
Results: Eight new non-homoeologous wheat genes were cloned and characterized. The nine PDI and PDI-like
sequences of wheat were located in chromosome regions syntenic to those in rice and assigned to eight plant
phylogenetic groups. The nine wheat genes differed in their sequences, genomic organization as well as in the domain
composition and architecture of their deduced proteins; conversely each of them showed high structural conservation
with genes from other plant species in the same phylogenetic group. The extensive quantitative RT-PCR analysis of the
nine genes in a set of 23 wheat samples, including tissues and developmental stages, showed their constitutive, even
though highly variable expression.
Conclusions: The nine wheat genes showed high diversity, while the members of each phylogenetic group were
highly conserved even between taxonomically distant plant species like the moss Physcomitrella patens. Although
constitutively expressed the nine wheat genes were characterized by different expression profiles reflecting their
different genomic organization, protein domain architecture and probably promoter sequences; the high conservation
among species indicated the ancient origin and diversification of the still evolving gene family. The comprehensive
structural and expression characterization of the complete set of PDI and PDI-like wheat genes represents a basis for
the functional characterization of this gene family in the hexaploid context of bread wheat
A multi agent system approach for hospital's drugs management using combinatorial auctions
No full textThe healthcare services cost are constantly increasing during the last 20 years. This trend has two origins: on one hand the people resort to drugs and healthcare services more often than in the past because more medical treatment are known; on the other hand, this evolution was not supported by an intelligent growth and design of healthcare structure and services. Indeed, a lot of hospital processes, such as drugs and equipments purchasing and managing, are not under control of hospital managers. This work faces the cost and management criticalities presenting a healthcare system as a multi-agent cooperative network. The proposed model faces the challenge of (i) gaining the benefits deriving from successful collaborative models already used in industrial systems and (ii) transferring the most appropriate industrial management practices. The model exploits an operations research technique based on combinatorial auctions in order to effective assign drugs to wards. © 2010 IEEE
Città ad arte: pratiche artistiche nello spazio urbano/Art and the City: artistic practices in the urban space
No full textIl saggio tratta della recente pratica artistica volta ad operare fuori dal museo, nello spazio pubblico come luogo fisico e come sistema di relazioni.Dipartimento di Architettura e Progetto dell'Università di Roma "La Sapienza"
Some notes on the use of tempera media in Nineteenth century Italy
No full textSi riassumono le ricerche sulla tecnica a tempera da parte degli artisti italiani tra Ottocento e Novecento, concentrando l'attenzione sui dipinti mobili e precisando la terminologia tecnica derivante dalle fonti documentarie.Researches on tempera technique used by italian painters during XIXth-early XX centuries are summarized putting attention on easel and canvas paintings. Italian technical terminology is specified by means citations from documentary sources
Note a margine di una recente ricerca sulla semplificazione amministrativa
No full textUna recente ricerca promossa dalla fondazione Astrid fornisce un quadro interessante delle politiche di semplificazione amministrativa in Italia, delle loro caratteristiche e delle innovazioni più importanti che sono state introdotte. Queste ultime sono fondamentalmente di sei tipi e riguardano, rispettivamente, lo sviluppo di una pluralità di vie amministrative; la rilevanza accordata ad una dimensione conoscitiva; la partecipazione dei privati; una caratterizzazione policentrica; l’europeizzazione e la convergenza tra gli ordinamenti nazionali degli Stati membri dell’Unione europea
Roman Sources for the History of American Catholicism
No full textultima bozza in wordOver the last decade, historians of American Catholicism have become increasingly aware that the Church in the United States cannot be properly understood without reference to its truly international character. One result of this is that scholars have become much more attentive to the relationship between the Roman Curia and the American Church
Phloem cytochemical modification and gene expression following the recovery of apple plants from apple proliferation
No full textL'articolo é disponibile sul sito dell'editore: http://www.apsjournals.apsnet.orgRecovery of apple trees from apple proliferation was studied by
combining ultrastructural, cytochemical, and gene expression analyses to
possibly reveal changes linked to recovery-associated resistance. When
compared with either healthy or visibly diseased plants, recovered apple
trees showed abnormal callose and phloem-protein accumulation in their
leaf phloem. Although cytochemical localization detected Ca2+ ions in the
phloem of all the three plant groups, Ca2+ concentration was remarkably
higher in the phloem cytosol of recovered trees. The expression patterns
of five genes encoding callose synthase and of four genes encoding
phloem proteins were analyzed by quantitative real-time reverse transcription-
polymerase chain reaction. In comparison to both healthy and
diseased plants, four of the above nine genes were remarkably upregulated
in recovered trees. As in infected apple trees, phytoplasma
disappear from the crown during winter, but persist in the roots, and it is
suggested that callose synthesis/deposition and phloem-protein plugging
of the sieve tubes would form physical barriers preventing the recolonization
of the crown during the following spring. Since callose deposition
and phloem-protein aggregation are both Ca2+-dependent processes, the
present results suggest that an inward flux of Ca2+ across the phloem
plasma membrane could act as a signal for activating defense reactions
leading to recovery in phytoplasma-infected apple trees