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Transcriptome and microtranscriptome analysis of small extracellular vesicles in uterine fluid from pregnancies produced by artificial insemination and in vitro fertilization in cattle
No full textA comunicação materno/embrio-fetal mediada por vesículas extracelulares pequenas (sEVs) desempenha um papel crucial no sucesso da gestação, facilitando o diálogo entre mãe e feto durante o desenvolvimento embrionário. A carga transportada por essas sEVs, incluindo pequenos e longos RNAs não codificantes (miRNAs e lncRNAs), bem como RNAs mensageiros (mRNAs), regula as células-alvo, traduzindo as necessidades do embrião ao mesmo tempo em que sinaliza ao sistema imune materno a tolerância ao novo organismo. Isolamos sEVs do fluido uterino de gestações bovinas produzidas por fertilização in vitro (FIV) e inseminação artificial (IA), nos dias 18 (pré-adesão embrionária) e 32 (pós-adesão embrionária). Ao analisar os mRNAs, microRNAs, e lncRNAs transportados por essas vesículas, buscamos compreender melhor seus mecanismos de ação, visando melhorar o desempenho da FIV em bovinos. O fluido uterino foi coletado, e a concentração e o tamanho das sEVs foram mensurados para cada técnica reprodutiva. O RNA total foi extraído para análises de transcriptoma (RNA-Seq) e microtranscriptoma (microarranjo), seguidas de análise de enriquecimento de conjuntos gênicos. Um total de 22 conjuntos foram enriquecidos por mRNAs em IVF18 x AI18, e 24 em IVF32 x AI32. Dentro das técnicas, 2 conjuntos foram enriquecidos em AI32 x AI18 e 30 em IVF32 x IVF18 (FDR≤0.005). Notavelmente, os caminhos de interação citocina-receptor de citocina e moléculas de adesão celular (IVF32 x AI32) foram enriquecidos. A análise da expressão diferencial de lncRNAs mostrou 398 lncRNAs regulados positivamente e 5 negativamente em IVF32 x AI32; 24 regulados positivamente e 25 negativamente em AI32 x AI18; e 1.081 regulados positivamente e 21 negativamente em IVF32 x IVF18. A análise microtranscriptômica revelou 5 miRNAs regulados negativamente em AI32 x AI18 e 11 regulados positivamente e 2 negativamente em IVF32 x IVF18. O sucesso da comunicação mediada por sEVs depende de sua carga. Observamos um aumento no número de miRNAs, lncRNAs e mRNAs diferencialmente expressos em gestações por FIV, tanto aos 18 quanto aos 32 dias, em comparação com IA. Além disso, identificamos genes codificadores de citocinas que podem prejudicar a receptividade uterina e a tolerância materno/embrio-fetal, bem como genes envolvidos na adesão embrionária em FIV. Adicionalmente, genes diferencialmente expressos associados à via de endocitose parecem promover a liberação de sEVs, o que está de acordo com nossas observações de maiores concentrações de sEVs aos 32 dias nas gestaçõespor FIV. Em resumo, as sEVs de gestações por IA e FIV apresentam conteúdos distintos de transcritos codificantes e não codificantes, o que pode estar relacionado a falhas na comunicação materno/embrio-fetal na FIV.Maternal/embryo-fetal communication mediated by small extracellular vesicles (sEVs) plays a crucial role in pregnancy outcomes, facilitating the dialogue between mother and fetus during embryonic development. The cargo transported by these sEVs—including small and long non-coding RNAs (miRNAs and lncRNAs), as well as messenger RNAs (mRNAs)—regulates target cells by conveying embryonic needs while also signaling the maternal immune system to tolerate the new organism. We isolated sEVs from the uterine fluid of bovine pregnancies produced by in vitro fertilization (IVF) and artificial insemination (AI) on days 18 (pre-embryo adhesion) and 32 (post-embryo adhesion). By analyzing the mRNAs, microRNAs, and lncRNAs carried by these vesicles, we aimed to better understand their mechanisms of action in order to improve pregnancy rates following IVF in cattle. Uterine fluid was collected, and the concentration and size of the sEVs were measured for each reproductive technique. Total RNA was extracted for transcriptome (RNA-Seq) and microtranscriptome (microarray) analyses, followed by gene set enrichment analysis. A total of 22 gene sets were enriched by mRNAs in IVF18 x AI18, and 24 in IVF32 x AI32 (FDR ≤ 0.005). Within techniques, 2 sets were enriched in AI32 x AI18, and 30 in IVF32 x IVF18. Notably, the cytokine–cytokine receptor interaction and cell adhesion molecule pathways (IVF32 x AI32) were enriched. Differential expression (DE) analysis of lncRNAs revealed 398 upregulated and 5 downregulated lncRNAs in IVF32 x AI32; 24 upregulated and 25 downregulated in AI32 x AI18; and 1,081 upregulated and 21 downregulated in IVF32 x IVF18. Microtranscriptomic analysis revealed 5 downregulated miRNAs in AI32 x AI18, and 11 upregulated and 2 downregulated in IVF32 x IVF18. The success of sEV-mediated communication depends on their cargo. We observed an increase in the number of differentially expressed miRNAs, lncRNAs, and mRNAs in IVF pregnancies on both days 18 and 32, compared to AI. Additionally, we identified cytokine-encoding genes that may impair uterine receptivity and maternal-fetal tolerance, as well as genes involved in embryo adhesion in IVF. Furthermore, differentially expressed genes associated with the endocytosis pathway appear to promote the release of sEVs, consistent with our observation of higher sEV concentrations on day 32 in IVF pregnancies. In summary, sEVs from AI and IVF pregnancies carry distinct coding and non-coding transcript content, which may be linked to failures in maternal/embryo-fetal communication in IVF
Bound states of massive complex ghosts in superrenormalizable quantum gravity theories
No full textOne of the remarkable differences between renormalizable quantum gravity with four-derivative action and its superrenormalizable polynomial generalizations is that the latter admit a more sophisticated particle mass spectrum. Already in the simplest superrenormalizable case, the theory has a six-derivative Lagrangian, admitting either a real or complex spectrum of masses. In the case of a real spectrum, there are the graviton, massive unphysical ghosts, and normal particles with masses exceeding the ones of the ghosts. It is also possible to have pairs of complex conjugate massive ghost-like particles. We show that in both cases, these theories do not admit a Källén-Lehmann representation and do not satisfy the positivity criterium of consistency in terms of the fields associated to those particles. In the main part of the work, using a relatively simple Euclidean scalar toy model, we show that the theory with complex spectrum forms bound states confining unphysical massive excitations into a normal composite particle. Finally, we discuss the cosmological implications of such a ghost confinement.Centro de Astropartículas y Física de Altas Energías Departamento de Física Teórica Universidad de ZaragozaInstituto de Física Teórica Universidade Estadual Paulista, Rua Dr. Bento Teobaldo Ferraz, 271 — Bloco II, SPDepartamento de Física ICE Universidade Federal de Juiz de Fora, Campus Universitário, MGInstituto de Física Teórica Universidade Estadual Paulista, Rua Dr. Bento Teobaldo Ferraz, 271 — Bloco II, S
Effect of thermal drying on the physico-chemical and microbiological characteristics of drinking water treatment sludge
No full textThe proper management and handling of drinking water treatment sludge (DWTS) is complex due to the quantity of sludge generated after treatment, the composition of the sludge, and the need to dry the material before its beneficial use or final disposal. Studies on the drying and feasibility of beneficial use of DWTS have been carried out on a laboratory scale, but few real-life applications have been proposed. This applied research is aimed at evaluating the effects of full-scale thermal drying on the physicochemical and microbiological characteristics of DWTS produced in Drinking Water Treatment Plants (DWTP), during two different climatic seasons (dry and rainy). For this purpose, the thermal drying process was described, and an exploratory analysis of the characteristics of the sludge before and after thermal drying was performed. Analyses were carried out on raw mass and on leached and solubilized extracts of DWTS samples, considering the Brazilian standard and the US standard for characterizing the risks to human and environmental health of waste. The results showed that the thermal drying process provided a reduction in the moisture content of DWTS of 62 % in the dry period and 74 % in the rainy season. It was also observed that thermal drying leads to the inactivation of microorganisms and that the resulting product brings benefits to the other stages of managing this waste. Furthermore, it also showed that the centrifuged sludges can be classified as non-hazardous and non-inert waste if the results of the analyses carried out on dry mass and solubilized extracts are considered. However, the presence of the elements: barium, chromium, and mercury, in the leached extracts, highlights the need for complementary toxicity testing for the proper classification of these wastes.Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)Universidade Estadual Paulista Júlio de Mesquita Filho Rodovia Presidente Dutra, Km 137,8 s/n Distrito de - Eugênio de Melo, São José dos Campos – SPUniversidade Estadual Paulista Júlio de Mesquita Filho Rodovia Presidente Dutra, Km 137,8 s/n Distrito de - Eugênio de Melo, São José dos Campos – S
Advances in forensic genetics: Exploring the potential of long read sequencing
No full textDNA-based technologies have been used in forensic practice since the mid-1980s. While PCR-based STR genotyping using Capillary Electrophoresis remains the gold standard for generating DNA profiles in routine casework worldwide, the research community is continually seeking alternative methods capable of providing additional information to enhance discrimination power or contribute with new investigative leads. Oxford Nanopore Technologies (ONT) and PacBio third-generation sequencing have revolutionized the field, offering real-time capabilities, single-molecule resolution, and long-read sequencing (LRS). ONT, the pioneer of nanopore sequencing, uses biological nanopores to analyze nucleic acids in real-time. Its devices have revolutionized sequencing and may represent an interesting alternative for forensic research and routine casework, given that it offers unparalleled flexibility in a portable size: it enables sequencing approaches that range widely from PCR-amplified short target regions (e.g., CODIS STRs) to PCR-free whole transcriptome or even ultra-long whole genome sequencing. Despite its higher error rate compared to Illumina sequencing, it can significantly improve accuracy in read alignment against a reference genome or de novo genome assembly. This is achieved by generating long contiguous sequences that correctly assemble repetitive sections and regions with structural variation. Moreover, it allows real-time determination of DNA methylation status from native DNA without the need for bisulfite conversion. LRS enables the analysis of thousands of markers at once, providing phasing information and eliminating the need for multiple assays. This maximizes the information retrieved from a single invaluable sample. In this review, we explore the potential use of LRS in different forensic genetics approaches.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Apoio à Pesquisa do Distrito FederalMolecular Genetics and Bioinformatics Laboratory Experimental Research Unit - Unipex School of Medicine São Paulo State University - Unesp, São PauloDepartamento de Química Laboratório de Pesquisas Forenses e Genômicas Faculdade de Filosofia Ciências e Letras de Ribeirão Preto Universidade de São Paulo, SPDepartamento de Genética Faculdade de Medicina de Ribeirão Preto Universidade de São Paulo, SPDepto Genética e Morfologia Instituto de Ciências Biológicas Universidade de Brasília, DFDepartment of Bioengineering Department of Physics Khoury College of Computer Sciences Northeastern UniversityPathology Department School of Medicine São Paulo State University - Unesp, São PauloMolecular Genetics and Bioinformatics Laboratory Experimental Research Unit - Unipex School of Medicine São Paulo State University - Unesp, São PauloPathology Department School of Medicine São Paulo State University - Unesp, São PauloCAPES: 001Fundação de Apoio à Pesquisa do Distrito Federal: 00193-00002596/2022-40CAPES: 307031/2022-5CAPES: 310016/2022-3CNPq: 408084/2023-
Do fungi look like macroparasites? Quantifying the patterns and mechanisms of aggregation for host-fungal parasite relationships
No full textMost hosts contain few parasites, whereas few hosts contain many. This pattern, known as aggregation, is well-documented in macroparasites where parasite intensity distribution among hosts affects host-parasite dynamics. Infection intensity also drives fungal disease dynamics, but we lack a basic understanding of host-fungal aggregation patterns, how they compare with macroparasites and if they reflect biological processes. To begin addressing these gaps, we characterized aggregation of the fungal pathogen Batrachochytrium dendrobatidis (Bd) in amphibian hosts. Utilizing the slope of Taylor's Power law, we found Bd intensity distributions were more aggregated than many macroparasites, conforming closely to lognormal distributions. We observed that Bd aggregation patterns are strongly correlated with known biological processes operating in amphibian populations, such as epizoological phase (i.e. invasion, post-invasion and enzootic), and intensity-dependent disease mortality. Using intensity-dependent mathematical models, we found evidence of evolution of host resistance based on aggregation shifts in systems persisting with Bd following disease-induced declines. Our results show that Bd aggregation is highly conserved across disparate systems and contains signatures of potential biological processes of amphibian-Bd systems. Our work can inform future modelling approaches and be extended to other fungal pathogens to elucidate host-fungal interactions and unite host-fungal dynamics under a common theoretical framework.School of Natural Resources University of Tennessee Institute of AgricultureUniversity of CaliforniaBiology Department Skidmore CollegeDepartment of Biology University of MississippiDepartment of Pathology Microbiology and Immunology Vanderbilt University School of MedicineDepartment of Biological Sciences Vanderbilt UniversityDepartment of Biological Sciences Pymatuning Laboratory of Ecology University of PittsburghDepartment of Biology University of MassachusettsSierra Nevada Aquatic Research Laboratory University of CaliforniaEarth Research Institute University of CaliforniaDepartment of Biodiversity and Aquaculture Center (CAUNESP) Universidade Estadual Paulista, SPDepartment of Biology The Pennsylvania State UniversityOne Health Microbiome Center Center for Infectious Disease Dynamics Ecology Institute Huck Institutes of the Life Sciences The Pennsylvania State UniversityUniversity of Colorado BoulderDepartment of Biodiversity and Aquaculture Center (CAUNESP) Universidade Estadual Paulista, S
A violência contra a mulher por parceiro íntimo nos serviços de Atenção Primária: da invisibilidade à medicalização
No full textThe aim of this study was to identify care strategies used by primary health care professionals in situations of violence against women. We conducted a qualitative study using primary data obtained from interviews with 16 women and secondary data from 14 patient medical records in women’s health services. The findings reveal that health professionals reaffirmed intimate partner violence as a domestic phenomenon limited to private settings and/or medicalized the problem with anti-inflammatory drugs and benzodiazepines. Violence against women was therefore either rendered invisible or medicalized, without ever considering a comprehensive approach to the concept of health and disease.Departamento de Saúde Pública Faculdade de Medicina de Botucatu Universidade Estadual Paulista “Julio de Mesquita Filho” (Unesp), Avenida Prof. Mário Rubens Guimarães Montenegro, S/N, SPDepartamento de Saúde Pública Faculdade de Medicina de Botucatu Universidade Estadual Paulista “Julio de Mesquita Filho” (Unesp), Avenida Prof. Mário Rubens Guimarães Montenegro, S/N, S
Dhvar5- and MSI78-coated titanium are bactericidal against methicillin-resistant Staphylococcus aureus, immunomodulatory and osteogenic
No full textInfection is one of the major issues associated with the failure of orthopedic devices, mainly due to implant bacterial colonization, biofilm formation, and associated antibiotic resistance. Antimicrobial peptides (AMP) are a promising alternative to conventional antibiotics given their broad-spectrum of activity, low propensity to induce bacterial resistance, and ability to modulate host immune responses. Dhvar5 (LLLFLLKKRKKRKY) and MSI78 (GIGKFLKKAKKFGKAFVKILKK) are two AMP with broad-spectrum activity against bacteria, including methicillin-resistant Staphylococcus aureus (MRSA), one of the most problematic etiologic agents in Orthopedic Devices-Related Infections (ODRI). This work aims to evaluate the bactericidal, immunomodulatory and osteogenic potential of Dhvar5- and MSI78-coated titanium surfaces (AMP-Ti). Two AMP-Ti surfaces were successfully obtained by grafting Dhvar5 (0.8 ± 0.1 µM/mm2) or MSI78 (0.5 ± 0.3 µM/mm2) onto titanium substrates through a polydopamine layer. Both AMP-Ti were bactericidal against MRSA, eradicating bacteria upon contact for 6 h in a culture medium supplemented with human plasma proteins. The AMP-Ti immunomodulatory potential was evaluated using human primary macrophages, by assessing surfaces capacity to induce pro-/anti-inflammatory (M1/M2) markers and cytokines. While in naïve conditions both AMP-Ti surfaces slightly promoted the M2 marker CD163, in response to LPS and IFN-γ (simulating a bacterial infection), both AMP increased the M1 marker CCR7 and enhanced macrophage secretion of pro-inflammatory IL-6 and TNF-α cytokines, particularly for Ti-MSI78 surfaces. Additionally, both AMP-Ti surfaces were cytocompatible and promoted osteoblastic cell differentiation. This proof-of-concept study demonstrated the high potential of Dhvar5- and MSI78-Ti as antimicrobial coatings for ODRI prevention. Statement of significance: This study investigates the bactericidal effects of the antimicrobial peptides Dhvar5 and MSI78, immobilized on titanium (Ti) surfaces through a polydopamine coating, aiming at the prevention of Orthopedic-Device Related Infections (ODRIs). The developed coatings displayed MRSA-sterilizing activity, while revealing an immunomodulatory potential towards macrophages and promoting osteoblastic cell differentiation. This strategy allows a quick and easy immobilization of high quantities of AMP, unlike most other approaches, thus favoring its clinical translation.i3S – Instituto de Investigação e Inovação em Saúde Universidade do PortoINEB – Instituto de Engenharia Biomédica Universidade do PortoFEUP–Faculdade de Engenharia Universidade do PortoICBAS – Instituto de Ciências Biomédicas Abel Salazar Universidade do PortoColégio Internato dos Carvalhos (CIC)Department of Microbiology and Parasitology University of NavarraNavarra Institute for Health Research (IdiSNA)UNESP - Universidade Estadual Paulista Faculdade de Ciências, SPNanoSafety Group International Iberian Nanotechnology LaboratoryUNESP - Universidade Estadual Paulista Faculdade de Ciências, S
The use of nano-structured cellulose to improve plywood: A review
No full textThe great availability of forest resources in Brazil enables the constant growth of industrial sectors that employ wood as a raw material. Hence, several products and production techniques were developed to maximize the availability of this resource, giving rise to wood-based panels and highlighting plywood as a structural use. The use of nanomaterials appears as an alternative to improve wood products. Among these nanomaterials, nano-structured cellulose has stood out for its high strength and stiffness. In this sense, this article aims to present a literature review regarding the use and effects of nanocellulose in plywood panels based on a systematic searching of keywords related to nano-structured cellulose in plywood employment on the Scopus website. An initial search from 1950 was performed with the keyword “cellulose.” A subsequent search included the acronym “plywood” combined with terms such as “nanocellulose,” “cellulose nanocrystals,” and “cellulose nanofibrils.” The Virtual Operating System (VOS) Viewer was used to develop a graphical mapping. The main authors were selected for an in-depth analysis of their results. Thus, it was concluded that most authors observed, for both nanocrystals and cellulose nanofibers, an increase in the adhesive viscosity as well as an improvement in the mechanical properties, especially regarding tensile shear strength and bonding quality, for 0.038, 1, 3, and 5% of nanocellulose. Some authors have also achieved a reduction in formaldehyde emissions. Therefore, using nanofibers and cellulose nanocrystals can be considered sustainable alternatives for the wood-based panel industry, improving the mechanical properties and reducing toxic emissions.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Engineering São Paulo State UniversityFaculty of Social and Agricultural Sciences of ItapevaMaterials Science Department of Military Institute of Engineering (IME)UENF - State University of the Northern Rio de Janeiro LECIV – Civil Engineering Laboratory, RJFederal University of São Carlos Dept. of Civil EngineeringEngineering São Paulo State UniversityCAPES: 00
Development of bioactive, bio-selective, and wear-resistant coatings on Ti-6Al-4V alloy produced by MAO for application as hip and knee implants
No full textA osteoartrite, agravada pelo envelhecimento e obesidade, tem aumentado a demanda por implantes ortopédicos mais duráveis e funcionais. A liga de titânio Ti-6Al-4V é amplamente utilizada para essas aplicações devido à sua excelente resistência mecânica, à corrosão e à biocompatibilidade. No entanto, o desgaste prolongado pode liberar íons de alumínio (Al) e vanádio (V), que são potencialmente tóxicos para o organismo. Este estudo teve como objetivo melhorar as propriedades da liga Ti-6Al-4V ELI através da aplicação de recobrimentos bioativos obtidos por Oxidação por Micro-Arco (Micro-Arc Oxidation – MAO), visando otimizar sua resistência ao desgaste e suas propriedades biológicas para implantes ortopédicos, como de Substituição Total de Quadril – Total Hip Replacement (THR) e Substituição Total de Joelho – Total Knee Replacement (TKR). Os recobrimentos foram obtidos utilizando eletrólitos contendo cálcio (Ca), fósforo (P) e óxidos de metais de transição (OMTs), como dióxido de titânio (TiO₂), óxido de alumínio (Al₂O₃), trióxido de molibdênio (MoO₃), óxido de ferro (Fe₂O₃) e dióxido de manganês (MnO₂), variando suas concentrações. As amostras foram tratadas sob uma tensão de 300 volts por 90 segundos e caracterizadas por várias técnicas analíticas, incluindo Microscopia Eletrônica de Varredura (MEV), Espectroscopia de Energia Dispersiva de Raios X (EDS), Espectroscopia no Infravermelho por Transformada de Fourier (FTIR), Espectroscopia Raman, Espectroscopia de Fotoelétrons por Raios X (XPS), Difração de Raios- X (DRX), Microscopia Eletrônica de Transmissão (MET), perfilometria e ensaios de microdureza Vickers. Os resultados indicaram que a adição dos OMTs influenciou positivamente a porosidade, a rugosidade e a composição química dos recobrimentos, promovendo melhorias na resistência ao desgaste e ação antimicrobiana contra diversos microrganismos, sem comprometer a viabilidade celular. A presença de TiO₂ e Ca e P nas camadas externas dos recobrimentos foi determinante para a biocompatibilidade, apresentando resultados satisfatórios em testes biológicos preliminares . Além disso, a estrutura porosa e a tendência hidrofílica dos recobrimentos contribuíram para uma interação mais eficiente com o meio biológico. Esses recobrimentos bioativos, enriquecidos com OMTs, mostraram-se promissores para aplicações em implantes ortopédicos, uma vez que combinaram a resistência ao desgaste com propriedades antimicrobianas e boa biocompatibilidade em relação ao substrato. A modificação estrutural e química proporcionada pela técnica MAO, especialmente com a adição de OMTs, pode oferecer uma alternativa eficaz para o desenvolvimento de implantes ortopédicos mais duráveis e funcionais, com o potencial de reduzir complicações pós-cirúrgicas e melhorar a longevidade dos dispositivos.Osteoarthritis, exacerbated by aging and obesity, has increased the demand for more durable and functional orthopedic implants. The Ti-6Al-4V titanium alloy is widely used for these applications due to its excellent mechanical strength, corrosion resistance, and biocompatibility. However, prolonged wear can release aluminum (Al) and vanadium (V) ions, which are potentially toxic to the body. This study aimed to enhance the properties of Ti-6Al-4V ELI alloy by applying bioactive coatings obtained through Micro-Arc Oxidation (MAO), thereby optimizing wear resistance and biological properties for orthopedic implants, such as Total Hip Replacement (THR) and Total Knee Replacement (TKR). The coatings were developed using electrolytes containing calcium (Ca), phosphorus (P), and transition metal oxides (TMOs), including titanium dioxide (TiO₂), aluminum oxide (Al₂O₃), molybdenum trioxide (MoO₃), iron oxide (Fe₂O₃), and manganese dioxide (MnO₂), with varying concentrations. The samples were treated at 300 volts for 90 seconds and characterized using multiple analytical techniques, including Scanning Electron Microscopy (SEM), Energy Dispersive X-ray Spectroscopy (EDS), Fourier Transform Infrared Spectroscopy (FTIR), Raman Spectroscopy, X-ray Photoelectron Spectroscopy (XPS), X-ray Diffraction (XRD), Transmission Electron Microscopy (TEM), profilometry, and Vickers microhardness testing. The results indicated that the addition of TMOs positively influenced the porosity, roughness, and chemical composition of the coatings, leading to improved wear resistance and antimicrobial activity against various microorganisms without compromising cell viability. The presence of TiO₂, Ca, and P in the outer layers of the coatings was crucial for biocompatibility, yielding satisfactory outcomes in preliminary biological tests. Additionally, the porous structure and hydrophilic tendency of the coatings contributed to more effective interaction with the biological environment. These bioactive coatings, enriched with TMOs, proved promising for orthopedic implant applications by combining enhanced wear resistance with antimicrobial properties and superior biocompatibility compared to the substrate. The structural and chemical modifications achieved through the MAO technique, particularly with the addition of TMOs, present an effective alternative for developing more durable and functional orthopedic implants, with the potential to reduce post-surgical complications and improve implant longevity.Coordenação de Aperfeiçoamento de Pessoal de Nível Superior (CAPES)Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq)Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP)88887.976789/2024-0088887.827655/2023-00Capes: 001407251/2018-9404020/2023-22018/24931-72021/13921-32024/03148-
Data collection process in the complex informational ecology of the Museum of Sexual Diversity
No full textObjective: To describe the data collection process within the scope of complex informational ecology, with a specific focus on the analog environments of the Museum of Sexual Diversity. Method: The ethnographic research methodology was adopted, allowing for deep immersion in the museum's context. Results: Through detailed observations, informal conversations, and semi-structured interviews, the researcher captured the dynamic interaction between visitors, staff, and museum exhibits. Conclusions: The collected data contribute to an in-depth analysis of sexual diversity issues and the role of the museum in contemporary society, highlighting the importance of appropriate methodological approaches to understanding the complexity of analog environments such as the Museum of Sexual Diversity.Univ Estadual Paulista, Dept Ciencia Informacao, Marilia, SP, BrazilUniv Estadual Paulista, Dept Ciencia Informacao, Marilia, SP, Brazi