Sivas Cumhuriyet Üniversitesi Kurumsal Akademik Arşivi - Sivas Cumhuriyet University Open Access System
Not a member yet
34175 research outputs found
Sort by
In Vivo Evidence for the Preventive Role of Vaccinium macrocarpon Aiton in Indomethacin-Induced Gastric Ulcer: Focusing on Antioxidant, Anti-Inflammatory and Anti-Apoptotic Mechanisms
No full textThe present study aimed to unveil the gastroprotective potential of Vaccinium macrocarpon (VM) extract and its mechanism of action against indomethacin (INDO)-induced gastric ulcers in rats. To achieve this goal, rats were pretreated with either omeprazole (20 mg/kg) or VM (100 mg/kg) orally for 14 consecutive days. Gastric tissue samples were collected and various parameters were evaluated to understand the mechanism of VM's action, including the levels of superoxide dismutase, malondialdehyde, glutathione, CAT and transforming growth factor beta (TGF-beta), as well as the mRNA expression levels of tumour necrosis factor alpha, interleukin 1 beta, nuclear factor kappa B (NF-kappa B) and inhibitor kappa B (I kappa B). Additionally, the immunopositivity of cyclooxygenase (COX)-1, COX-2, PGE2, proliferating cell nuclear antigen (PCNA) and caspase-3 was assessed. The total amount of phenolic compounds present in the VM extract was high (58.08 mu g/mL gallic acid equivalent/mg extract). The healing effect of VM was demonstrated by an increase in the expression of PCNA. Furthermore, the level of TGF-beta was found to increase upon treatment with VM. Analyses of COX-1, COX-2 and PGE2 expression in gastric tissue confirmed the gastroprotective effect of VM. Notably, the expression of NF-kappa B was markedly reduced, whereas that of I kappa B was substantially increased. Overall, the findings of this study demonstrate that VM extract has gastroprotective and curative effects against INDO-induced ulcers through its antioxidant, anti-inflammatory, mucosal regenerative and anti-apoptotic activities. Therefore, VM may serve as a useful adjuvant treatment for nonsteroidal anti-inflammatory drugs-induced gastric ulcer disease
Exploring the Prognostic Role of Trop-2, CD47, and CD163 Expression Levels on Survival Outcomes in Patients with Triple-Negative Breast Cancer
No full textBackground: This study evaluated the prognostic impact of Trop-2, CD47, and CD163 expression on clinical outcomes in triple-negative breast cancer (TNBC) and investigated their interactions with tumor progression. Methods: A retrospective cohort of 92 patients with TNBC was analyzed. The expression scores for Trop-2, CD47, and CD163 were categorized as negative/low (0-3 points) or high (4-6 points). The primary endpoint was overall survival (OS). Results: The median age of the cohort was 50 years old. High Trop-2 expression was observed in 55.4% of the patients and was significantly associated with advanced disease stage (p < 0.001). High CD47 expression (44.6%) was correlated with advanced stage (p = 0.044), whereas high CD163 expression (45.7%) was associated with advanced stage (p = 0.021), absence of comorbidities (p = 0.022), and lower pT stage (p = 0.023). Moderate positive correlations were found between Trop-2 and CD47 (p = 0.022), Trop-2 and CD163 (p = 0.037), and CD47 and CD163 (p < 0.001), respectively. Kaplan-Meier survival analysis revealed that patients with low Trop-2 expression exhibited significantly prolonged OS (p = 0.021) and progression-free survival (PFS) (p = 0.026) compared to those with high Trop-2 expression. Univariate and multivariate analyses revealed significant associations between OS and PFS for Trop-2, lymphovascular invasion, and BRCA status. Conclusions: Trop-2 expression is a significant prognostic factor for TNBC and is correlated with worse outcomes. Although CD47 and CD163 showed trends for poorer prognosis, their significance was not confirmed. These findings offer promising prospects for future studies on combined antibody-drug conjugates (ADCs), as they may present opportunities to address multiple resistance mechanisms in the management of TNBC and enhance clinical outcomes
Acceptable esthetic limits for maxillary dental midline shift and mandibular asymmetry: Who notices what and how much?
No full textIntroduction: This study aimed to evaluate maxillary dental midline shifts and mandibular asymmetries created in different amounts and directions on photographs taken from 7 different angles by different groups and to determine acceptable esthetic limits. Methods: Photographs of a female model in a social smile position were taken from 7 different angles (0 degrees [frontal], 15 degrees, 30 degrees, and 45 degrees on the right and left sides) and digitally modified for maxillary dental and mandibular midline deviations at specified degrees using Adobe Photoshop. To enable participants to evaluate the photographs sequentially, the photographs were transformed into videos using Adobe Premiere Pro software. The videos were rated by 188 participants (48 orthodontists; 49 dentists; 45 esthetic, plastic, and reconstructive surgeons; and 46 laypersons) using the visual analog scale (VAS) in a survey. An analysis of variance test was used to compare VAS scores among raters, and the Bonferroni test was used to compare VAS scores among the groups. Results: The threshold for the acceptability of maxillary dental midline shifts was 2 mm for orthodontists; dentists; and esthetic, plastic, and reconstructive surgeons but 4 mm for laypersons. Although laypersons were unable to perceive changes in mandibular asymmetry between 0 degrees-6 degrees, the threshold was 3 degrees for other groups. For participants in which mandibular asymmetries and maxillary dental midline shifts were in opposite directions, the threshold for all groups was 6 degrees. For participants in which lower jaw asymmetries and maxillary dental midline shifts were in the same direction, the threshold value for orthodontists was 6 degrees. Other groups could not perceive variables related to asymmetry. Conclusions: More sensitive esthetic assessments can be made by evaluating smile esthetics from different angles. Mandibular asymmetries are considered more esthetically acceptable than maxillary dental midline shifts. In mandibular asymmetry and maxillary dental midline shifts, deviations in the same direction are more esthetically acceptable than deviations in opposite directions. Orthodontists are able to notice small changes in mandibular asymmetry. (Am J Orthod Dentofacial Orthop 2025;167:425-35
SOKRATES VE DEMOKRASİNİN GERİLİMLERİ: BRENTON'UN CANCELLİNG SOCRATES ADLI OYUNU
No full textThis study explores the complex interplay between personal and political spheres as depicted in Howard Brenton's play Cancelling Socrates, alongside historical accounts of Socrates' trial and execution. Brenton’s play highlights the tension between individual values and societal demands through the contrasting perspectives of Xanthippe and Aspasia. Xanthippe’s dismissal of state concerns in favour of personal, familial realities underscores the clash between individual desires and political responsibilities. Aspasia’s emphasis on the state as the embodiment of the common good presents the democratic ideal in opposition to Xanthippe’s private sphere. This dynamic reflects the broader historical context of Socrates’ trial, where his philosophical inquiries and perceived corruption of Athenian youth provoked a democratic system struggling with internal contradictions. The study examines how Socrates’ role as a moral and intellectual provocateur challenged the democratic norms of his time, leading to his eventual prosecution. It also considers Brenton’s critique of civilization’s reliance on collective beliefs and norms, as articulated through Euthyphro's reflections in the play. This analysis reveals the enduring relevance of Socratic themes in understanding the tensions between personal integrity and political authority.Bu çalışma, Howard Brenton’un Sokrates’in İptali adlı oyununda tasvir edilen kişisel ve siyasi alanlar arasındaki karmaşık etkileşimi, Sokrates’in yargılanması ve idamıyla ilgili tarihsel anlatımlar bağlamında incelemektedir. Brenton’un oyununda Xanthippe ve Aspasia’nın zıt perspektifleri aracılığıyla bireysel değerler ile toplumsal talepler arasındaki gerilim vurgulanmaktadır. Xanthippe’nin kişisel ve ailevi gerçeklikler lehine devlet kaygılarını reddi, bireysel arzular ile siyasi sorumluluklar arasındaki çatışmayı altını çizmektedir. Aspasia’nın devleti ortak çıkarın somutlaşmış hali olarak vurgulaması, demokratik ideali Xanthippe’nin özel alanına karşıt olarak sunmaktadır. Bu dinamik, Sokrates’in yargılanmasının daha geniş tarihsel bağlamını yansıtmaktadır; burada Sokrates’in ahlaki ve entelektüel kışkırtıcılığı, iç çelişkilerle boğuşan bir demokrasiyi provoke etmiştir. Çalışma, Sokrates’in bir ahlaki ve entelektüel provokatör olarak nasıl demokratik normları sorguladığını ve nihai olarak yargılanmasına yol açtığını incelemektedir. Ayrıca, Brenton’un oyundaki Euthyphro aracılığıyla ifade edilen medeniyetin kolektif inanç ve normlara bağımlılığı üzerine eleştirisini değerlendirmektedir. Bu analiz, kişisel bütünlük ile siyasi otorite arasındaki gerilimleri anlamak bağlamında Sokratik temaların sürekliliğini ortaya koymaktadır
Spectrophotometric determination of chlorpyrifos in foodstuffs after vortex-assisted surfactant-enhanced emulsification microextraction using magnetic deep eutectic solvents: Analytical greenness profile
No full textIn this work a novel and green vortex-assisted surfactant-enhanced emulsification microextraction (VA-SEEME) based on magnetic deep eutectic solvents (MDESs) was developed for the determination of chlorpyrifos in foodstuffs by UV-Vis spectrophotometric analysis. MDES (trihexyltetradecylphosphonium chloride: MnCl2: octanoic acid) was used as the extractant, non-ionic surfactant Tween-80 was used as an extraction medium, and ethanol as the eluent solvent. In the VA-MDES-SEEME procedure, a vortex was applied for agitation, and a neodymium magnet was used to separate the MDES from the aqueous solution. Under optimized conditions, a linear dynamic range was obtained in the range of 0.3-260 mu g L- 1 with a detection limit of 0.09 mu g L- 1. The intraday and interday precisions of the method, defined as relative standard deviation, ranged from 1.3 % to 2.5 %. The recovery of the spiked sample after the VA-MDES-SEEME procedure ranged from 88+6 % to 98+1 %, and the enrichment factor was obtained as 91-fold. The VA-MDES-SEEME procedure was validated by analysis of the certified reference materials including 7507a-Green Onion and 7508a-Cabbage. The VA-MDES-SEEME procedure is based on inexpensive equipment and green chemicals and is compatible with UV-Vis spectrophotometer analysis. The green profile of the VA-MDES-SEEME procedure within the scope of the current study was calculated as 0.71 using AGREE
Bromelain Protects Against PTZ-Induced Glial Damage and Inflammation: An In Vitro and In Silico Study
No full textThis study aimed to investigate how bromelain protects glial cells from pentylenetetrazole (PTZ)-induced damage, focusing on its anti-inflammatory effects. C6 glioma cells were treated with PTZ, bromelain, or a combination of PTZ and bromelain. The interactions of bromelain with iNOS (Inducible Nitric Oxide Synthase) and COX2 (Cyclooxygenase-2) were investigated using molecular docking calculations. Cell viability was measured using the XTT (Methoxynitrosulfophenyl-Tetrazolium Carboxanilide) assay. iNOS, NO (Nitric Oxide), and COX2 levels were assessed using ELISA and immunofluorescence staining. Bromelain at 50 and 100 mu g/mL significantly increased cell viability (p < 0.001). On the other hand, bromelain at 50 g/mL reduced inflammation, as indicated by lower levels of NO, iNOS, and COX2 (p < 0.001). In-silico predictions suggest that bromelain can effectively target iNOS and COX2, key inflammatory proteins. These findings indicate that bromelain protects glial cells by exerting anti-inflammatory effects. However, further research is needed to understand the underlying mechanisms fully
Effect of herb powder addition in gluten-free bread production with black chickpea flour on technological and nutritional properties of bread
No full textÇölyak hastalığı; buğday gluteni ve diğer tahıllardaki benzer proteinlerin tüketilmesi sonucunda ortaya çıkan bir gıda intoleransıdır. Glutensiz ürünlerde gözlemlenen hacim azlığı, tekstür sertliği, lezzet kaybı, besin içeriğindeki düşüklük nedeniyle bu konuda araştırmalar yapılmaktadır. Bu tez çalışmasında; glutensiz ekmek üretiminde bitki tozlarının kullanılması ile ekmeğin teknolojik ve besinsel değerleri üzerine etkisi araştırılmıştır. Kullanılan bitki tozları çemen otu, bamya kurusu ve ayva çekirdeğidir. Seçilmiş olan bu bitki tozları tek veya birbiriyle olan kombinasyonlarıyla birlikte glutensiz ekmek yapımında kullanılmıştır. Bitki tozu ilave edilen glutensiz ekmeklerin teknolojik özelliklerinin analiz sonuçlarına göre; örneklerin ortalama yükseklik değerleri 2,87 ile 3,11 cm, spesifik hacim değerleri değerleri 2,36±0,04 cm3/g ile 4,23±0,18 cm3/g, ekmek örneklerinin iç nem değerleri %43,96±2,11 ile %49,64±2,55, kabuktaki kabuk nem değerleri %17,11±0,41 ile %25,11±0,27, toplam nem değerleri %32,49±3,24 ile %33,71±2,08, kabuk a* değerleri 11,14±1,79 ile 12,98±1,08, kabuk b*değerleri 19,38±0,36 ile 23,15±0,69 arasında değişim göstermektedir. SEM analiz sonuçları bitki tozu ilavesinin ekmek örneklerinde daha yüksek gözeneklilik oluşmasına neden olduğunu; özellikle HD2 (%100 bamya kurusu), HD6 (%50 bamya kurusu - %50 ayva çekirdeği), HD7 (%33,3 çemen otu - %33,3 bamya kurusu - %33,3 ayva çekirdeği) en yüksek gözenekliliğine sahip örnekler olduğunu göstermiştir. Bitki tozu ilave edilen glutensiz ekmeklerin besin içerikleri incelendiğinde; ortalama kül değerlerinin %0,85±0,17 ile 1,74±0,08, protein değerlerinin %9,78±0,17 ile %10,74±1,44, yağ değerlerinin %2,37±0,54 ile %5,61±0,33, karbonhidrat değerlerinin 48,49±0,24 ile 52,84±1,38, enerji değerlerin 265,02±12,95 kcal ile 286,28±5,40 kcal, diyet lif değerleri %5,95±0,48 - %6,97±0,4 arasında değişiklik gösterdiği belirlenmiştir. Bitki tozu ilave edilen örneklerin ortalama hızlı sindirilebilir nişasta (HSN) değerleri 39,27±1,78 g/100 g KM ile 42,99±0,57 g/100 g KM, yavaş sindirilebilir nişasta (YSN) değerleri 18,43±1,23 g/100 g KM ile 27,45±1,03 g/100 g KM, toplam sindirilebilir nişasta (TSN) değerleri 25,92±1,55 g/100 g KM ile 59,49±1,24 g/100 g KM, dirençli nişasta (DN) değerleri 2,65±0,24 g/100 g KM ile 6,25±0,89 g/100 g KM, toplam nişasta (TN) değerleri 30,31±1,06 g/100 g KM ile 55,31±13,04 g/100 g KM arasında tespit edilmiştir. Örneklerin tahmini glisemik indeks (tGI) içerikleri 88,06 ile 100,56 arasında belirlenmiştir. Duyusal analiz sonuçlarına göre, panelistler tarafından en yüksek genel beğeni puanını HD2 kodlu (%100 bamya kurusu), en düşük genel beğeni puanını HD4 kodlu (%50 çemen otu tozu, %50 bamya kurusu) glutensiz ekmek örneğinin aldığı görülmüştür. Bitki tozu ilaveli glutensiz ekmek örneklerinin hepsinde 1016-1018 cm-1, 1742-1744 cm-1 ve 3272-3279 cm-1 de yüksek pikler gözlenmiştir. Elde edilen sonuçlar değerlendirildiğinde, ekmeklerde bamya kurusu ilavesinin teknolojik ve besinsel olarak faydalı olduğu, duyusal analizlerde de panelistler tarafından bamya kurusu ilavesinin genel beğeniyi aldığı belirlenmiştir.Celiac disease is a food intolerance that occurs as a result of consuming wheat gluten and similar proteins in other grains. Research is being conducted on this subject due to the lack of volume, firm texture, loss of flavor, and low nutritional content observed in gluten-free products. In this thesis, the use of plant powders in gluten-free bread production and their effects on the technological and nutritional values of bread were investigated. The plant powders used were fenugreek, dried okra, and quince seeds. These selected plant powders were used alone or in combination with each other in the production of gluten-free bread. According to the analysis results of the technological properties of gluten-free breads to which plant powder was added; the average height values of the samples varied between 2.87 and 3.11 cm, specific volume values varied between 2.36±0.04 cm3/g and 4.23±0.18 cm3/g, internal moisture values of the bread samples varied between 43.96±2.11% and 49.64±2.55%, crust moisture values in the crust varied between 17.11±0.41% and 25.11±0.27%, total moisture values varied between 32.49±3.24% and 33.71±2.08%, crust a* values varied between 11.14±1.79 and 12.98±1.08, crust b* values varied between 19.38±0.36 and 23.15±0.69. The results of the SEM analysis revealed that the addition of plant powder increased the porosity in the bread samples, particularly in HD2 (100% dried okra), HD6 (50% dried okra - 50% quince seed), and HD7 (33.3% fenugreek - 33.3% dried okra - 33.3% quince seed). It was determined that average ash values varied between 0.85±0.17% and 1.74±0.08%, protein values varied between 9.78±0.17% and 10.74±1.44%, fat values varied between 2.37±0.54% and 5.61±0.33%, carbohydrate values varied between 48.49±0.24% and 52.84±1.38, energy values varied between 265.02±12.95 kcal and 286.28±5.40 kcal, and dietary fiber values varied between 5.95±0.48% and 6.97±0.4%. The average rapidly digestible starch (RDS) values of the samples with added plant powder were 39.27±1.78 g/100 g DM and 42.99±0.57 g/100 g DM, slowly digestible starch (SDS) values were 18.43±1.23 g/100 g DM and 27.45±1.03 g/100 g DM, total digestible starch (TSS) values were 25.92±1.55 g/100 g DM and 59.49±1.24 g/100 g DM, resistant starch (RS) values were 2.65±0.24 g/100 g DM and 6.25±0.89 g/100 g DM, total starch (TS) values were 30.31±1.06 g/100 g DM and 55.31±13.04 g/100 g DM was determined. The estimated glycemic index (tGI) contents of the samples were determined to be between 88.06 and 100.56. According to the sensory analysis results, it was seen that the HD2-coded (%100 dried okra) gluten-free bread sample received the highest general appreciation score from the panelists, and the HD4-coded (%50 fenugreek powder and %50 dried okra) gluten-free bread sample received the lowest general appreciation score. High peaks were observed at 1016-1018 cm⁻¹, 1742-1744 cm⁻¹, and 3272-3279 cm⁻¹ in all gluten-free bread samples with plant powder addition. It was determined that the addition of dried okra in breads was technologically and nutritionally beneficial, and the sensory analysis also determined that the addition of dried okra received general appreciation by the panelists
Evaluation self- management and quality of life in patients with epilepsy
No full textAraştırma, epilepsi tanısı almış bireylerde öz-yönetim ve yaşam kalitesinin değerlendirilmesi amacıyla tanımlayıcı ve analitik olarak gerçekleştirilmiştir. Araştırma, Sivas il merkezinde bulunan Sivas Numune Hastanesinde nöroloji polikliniğine başvuran epilepsi tanılı hastalar arasından rastgele örnekleme yöntemi ile seçilen 131 epilepsi hastası ile Ağustos 2023- Ocak 2024 tarihleri arasında gerçekleştirilmiştir. Araştırmanın verileri, Hasta Tanılama Formu, Epilepsi Hastalarında Öz-Yönetim Ölçeği (EÖYÖ) ve Epilepsili Hastalarda Yaşam Kalitesi Ölçeği (QOLIE-31)'ni içeren veri toplama araçları kullanılarak elde edilmiştir. Araştırmanın yapılabilmesi için veri toplama öncesinde etik kurul onayı ile yazılı kurum izinleri alınmıştır. Araştırma verilerinin değerlendirilmesinde, SPSS 23.0 programı kullanılarak tanımlayıcı istatistik testleri ile sayı ve yüzdelik dağılımlar ile ortalama, standart sapma, maksimum ve minimum değerlerin analizi, Bağımsız Gruplarda t testi, ANOVA varyans analizi, Tukey Post Hoc testi, Levene Testi, Pearson Korelasyon Katsayısı Analizi ve Lineer Regresyon analizi kullanılmıştır. Verilerin değerlendirilmesinde p<0.05 değeri anlamlı kabul edilmiştir. Araştırmaya katılan epilepsili hastalarının %51,9 'unun kadın, %51,1'inin evli, %42'sinin ilkokul mezunu, %87'sinin çalışmadığı, %83,2'sinin şehirde yaşadığı, %58'inin eş ve/veya çocukları ile beraber yaşadığı, %61,1'inin gelir düzeyinin kötü olduğu ve yaş ortalamasının 45,89±18,31 olduğu belirlenmiştir. Epilepsi hastalarının EÖYÖ ortalama puanı 104,73±10,99 olup orta düzeyde öz-yönetime sahip oldukları ve QOLIE-31'den ise ortalama 49,11±22,76 puan ile orta düzeyden düşük yaşam kalitesine sahip oldukları saptanmıştır. Epilepsi hastalarında öz-yönetim ile yaşam kalitesi düzeyi arasında orta seviyede ve pozitif yönde anlamlı ilişki olduğu; epilepsi hastalarında öz-yönetim düzeyi arttıkça yaşam kalitesi düzeyinin anlamlı düzeyde arttığı ve öz-yönetimde bir birimlik artışın yaşam kalitesi düzeyinde %42'lik bir artış sağladığı belirlenmiştir. Epilepsi hastalarının eğitim düzeyi, çalışma durumu, gelir düzeyi, nöbet tipi, nöbet sıklığı ve tedavi rejimi hem öz-yönetim hem yaşam kalitesi düzeyi açısından anlamlı fark bulunurken, medeni durum ve birlikte yaşanılan kişiler öz-yönetim düzeyi açısından anlamlı farklılık gösterdiği saptanmıştır. Sonuç olarak, epilepsi hastalarının yaşam kalitelerinin orta düzeyin altında olduğu ve orta düzey öz-yönetime sahip oldukları belirlenmiştir. Epilepsi hastalarında öz-yönetim ile yaşam kalitesi düzeyi arasında anlamlı düzeyde ilişki bulunduğu, hastaların öz-yönetim düzeyindeki artışın yaşam kalitesini olumlu şekilde etkilediği belirlenmiştir.This descriptive and analytical study was conducted to evaluate self-management and quality of life in individuals diagnosed with epilepsy. The study was conducted between August 2023 and January 2024 with 131 epilepsy patients selected by random sampling method among patients diagnosed with epilepsy who applied to the neurology outpatient clinic at Sivas Numune Hospital in Sivas provincial center. The data of the study were obtained by using data collection tools including Patient Identification Form, Self-Management Scale in Epilepsy Patients (SMSE) and Quality of Life in Patients with Epilepsy Scale (QOLIE-31). Ethics committee approval and written institutional permissions were obtained prior to data collection. In the evaluation of the research data, descriptive statistical tests, number and percentage distributions, mean, standard deviation, analysis of maximum and minimum values, Independent Groups t test, ANOVA analysis of variance, Tukey Post Hoc test, Levene Test, Pearson Correlation Coefficient Analysis and Linear Regression analysis were used using SPSS 23.0 program. A value of p<0.05 was considered significant in the evaluation of the data. It was determined that 51.9% of the patients with epilepsy who participated in the study were female, 51.1% were married, 42% were primary school graduates, 87% were not working, 83.2% lived in the city, 58% lived with their spouses and/or children, 61.1% had a poor income level and the mean age was 45.89±18.31. Epilepsy patients were found to have moderate self-management with a mean score of 104.73±10.99 on the ESLS and a lower than moderate quality of life with a mean score of 49.11±22.76 on the QOLIE-31. It was determined that there was a moderate and positive significant relationship between self-management and quality of life level in epilepsy patients; as the level of self-management increased in epilepsy patients, the level of quality of life increased significantly and a one-unit increase in self-management provided a 42% increase in quality of life level. While education level, employment status, income level, seizure type, seizure frequency and treatment regimen of epilepsy patients showed a significant difference in terms of both self-management and quality of life level, marital status and cohabitants showed a significant difference in terms of self-management level. As a result, it was determined that the quality of life of epilepsy patients was below medium level and they had medium level self-management. It was determined that there was a significant relationship between self-management and quality of life level in epilepsy patients and that the increase in the self-management level of patients positively affected the quality of life
Black Garlic Extract Modulates Endothelin Expression and Ovulatory Function in Monosodium Glutamate Treated Rats
No full textMonosodium glutamate (MSG), a widely used food additive, has been associated with various health concerns, including potential reproductive toxicity. This study investigated the protective effects of black garlic (BG) ethanol extract against MSG-induced ovarian damage in rats. Thirty-two female rats in estrus were randomly divided into four groups (n = 8 per group): control (saline), BG (250 mg/kg BW), MSG (4 mg/g BW), and BG+MSG (combined treatment). Treatments were administered daily for 14 days. Ovarian tissues were collected for histopathological, immunohistochemical (IHC), and biochemical analyses. Histopathological examination revealed a significant reduction in cystic follicles in the BG+MSG group compared to the MSG group (p < 0.0001). IHC analysis showed decreased immunoreactivity of endothelin-1 and endothelin-2 in the BG+MSG group compared to the MSG group (both p < 0.01). Biochemical assays demonstrated significantly increased follicle-stimulating hormone (FSH), luteinizing hormone (LH), and estradiol levels in the BG+MSG group compared to the MSG group (all p < 0.05), while progesterone levels were significantly lower in the MSG group compared to the BG+MSG group (p < 0.05). These findings suggest that BG ethanol extract may mitigate MSG-induced ovarian dysfunction in rats by alleviating degenerative changes in follicles and modulating hormonal levels. This study provides insights into potential natural interventions for MSG-related reproductive toxicity.Scientific and Technological Research Council of Turkey (TUBITAK)This study was granted open access permission by The Scientific and Technological Research Council of Turkey (TUBITAK)
Isolation of Traditional Yogurt Yeast Kluyveromyces marxianus and Investigation of Major Probiotic Properties
No full textF2022658The aim of this study was to evaluate the probiotic potential of Kluyveromyces marxianus K3 yeast strain isolated from cow yogurt. Kluyveromyces marxianus yeast strain was isolated from yogurt and identified based on ITS sequences. The isolated strain showed high tolerance to low pH conditions. This feature can be associated with the ability of the strain to survive in acidic environments such as stomach acid. In addition, the strain showed tolerance to simulated human gastric and intestinal fluids, indicating that it can move healthily in the digestive system. It was determined that the isolate was resistant to antibiotics, indicating its potential to help protect the intestinal flora. It was determined that the strain grew well at human body temperature, exhibited hydrophobic properties and had deconjugation ability against bile salts. The research findings indicate that Kluyveromyces marxianus strain obtained from yogurt has the potential to be used as a probiotic in different food products. This species is an important candidate that can be included in food supplements or new probiotic formulations. In conclusion, this study reveals that yogurt is a rich source of probiotic yeast species and Kluyveromyces marxianus K3 strain may play an important role in this field[No abstract available]Scientific Research Projects of Sivas Cumhuriyet Universit