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Inactivation of Nosema spp. with zinc phthalocyanine
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Most honey bee pathogens, such as Vairimorpha (Nosema), cannot be rapidly and definitively diagnosed in a
natural setting, consequently there is typically the spread of these diseases through shared and re-use of
beekeeping equipment. Furthermore, there are no viable treatment options available for Nosema spores to aid in
managing the spread of this bee disease. We therefore aimed to develop a new method using novel Zinc
Phthalocyanine (ZnPc) as a photosensitizer for the photodynamic inactivation of Nosema spores that could be
used for the decontamination of beekeeping equipment. Nosema spores were propagated for in vitro testing using
four caged Apis mellifera honey bees. The ZnPc treatment was characterized, encapsulated with a liposome, and
then used as either a 10 or 100 µM treatment for the freshly harvested Nosema spores, for either a 30 and or 60-
minute time period, under either light or dark conditions, in-vitro, in 96-well plates. In the dark treatment, after
30-min, the ZnPc 100 µM treatment, caused a 30 % Nosema mortality, while this increased to 80 % at the same
concentration after the light treatment. The high rate of anti-spore effects, in a short period of time, supports the
notion that this could be an effective treatment for managing honey bee Nosema infections in the future. Our
results also suggest that the photo activation of the treatment could be applied in the field setting and this would
increase the sterilization of beekeeping equipment against Nosem
Scan accuracy of wireless intraoral scanners while digitizing a combined scan body-healing abutment system
No full textAbstract
Objectives: To investigate the accuracy (trueness and precision) of wireless and wired intraoral scanners (IOSs) when scanning an implant with the combined healing abutment-scan body (CHA-SB) system.
Methods: A partially edentulous mandibular model with a CHA-SB at the right first molar site was digitized with 2 wireless (NeoScan 2000 (NW) and TRIOS 4 wireless (T4 W)) and 2 wired (NeoScan 1000 (N) and TRIOS 4 wired (T4)) IOSs 44 times in total (n = 11). The reference scan file was generated by digitizing the same master model and CHA-SB with an industrial-grade optical scanner. All files were imported into a metrology-grade analysis software program to evaluate the surface (root mean square, RMS), linear, and angular deviations of the top part of the SB. The average deviation values defined the precision of the scans. The data were statistically analyzed (α = 0.05).
Results: IOS type affected the surface and angular deviations and the precision (linear deviations on the x-axis) of the scans (p ≤ 0.043). T4 W had lower RMS than N and T4 (p ≤ 0.031). T4 had higher angular deviations than N on the XZ plane and had the lowest angular deviations on the YZ plane (p ≤ 0.011). T4 W scans had higher precision than N scans (p = 0.024).
Conclusion: Despite some differences in the trueness or precision of scans, tested IOSs mostly enabled similar scan accuracy. Regardless of the IOS, the implant scans had a tendency to tilt mesiobuccally, which can be considered clinically small.
Clinical significance: Tested wireless intraoral scanners may be suitable alternatives to their wired counterparts while digitizing an implant with a combined healing abutment-scan body system in the posterior region. However, the crowns fabricated from tested scans might require buccal, mesial, and occlusal surface veneering and distal surface adjustments
Characterization of speech and language phenotype in the 8p23.1 syndrome
No full textAbstract
The 8p23.1 duplication syndrome is a rare genetic condition with an estimated prevalence rate of 1 out of 58,000. Although the syndrome was associated with speech and language delays, a comprehensive assessment of speech and language functions has not been undertaken in this population. To address this issue, the present study reports rigorous speech and language, in addition to oral-facial and developmental, assessment of a 50-month-old Turkish-speaking boy who was diagnosed with the 8p23.1 duplication syndrome. Standardized tests of development, articulation and phonology, receptive and expressive language and a language sample analysis were administered to characterize speech and language skills in the patient. The language sample was obtained in an ecologically valid, free play and conversation context. The language sample was then analyzed and compared to a database of age-matched typically-developing children (n = 33) in terms of intelligibility, morphosyntax, semantics/vocabulary, discourse, verbal facility and percentage of errors at word and utterance levels. The results revealed mild to severe problems in articulation and phonology, receptive and expressive language skills, and morphosyntax (mean length of utterance in morphemes). Future research with larger sample sizes and employing detailed speech and language assessment is needed to delineate the speech and language profile in individuals with the 8p23.1 duplication syndrome, which will guide targeted speech and language interventions
One-step chronoamperometric synthesis of S, N co-doped graphene oxide electrodes for nonenzymatic electrochemical H2O2 sensor
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In this study, an S, N-codoped graphene oxide electrode was synthesised by a one-step chronoamperometric method to modify a pencil graphite electrode (PGE) for the non-enzymatic detection of H2O2. The electrochemical characterisation of modified PGE was carried out by means of cyclic voltammetry (CV), chronoamperometry (CA) and electrochemical impedance spectroscopy (EIS). The morphology and structural properties of this electrode was investigated by FTIR, XPS, XRD, Raman and SEM analysis. The S, N-codoped graphene oxide electrode has a high sensitivity with linear range of 0.1-90 mu M with correlation coefficient (R2 = 0.9959) and a low detection limit with a concentration of 0.015 mu Mf. Real sample analyses of the sensor, whose efficiency was investigated for practical application, were performed on real sample. The novelty of this study compared to the literature is the use of S,N Co-doped GO electrode synthesised in a single step as a nonenzymatic sensor for H2O2, achieving high sensitivity and low detection limit. Furthermore, this study presents the production of graphene oxide electrodes co-doped with sulfur and nitrogen in a single step, utilising the chronoamperometry method, which is a rapid, straightforward, and cost-effective process. Concurrently, the formation of graphene layers on the PGE surface was ensured, while oxygenated functional groups containing sulfur and nitrogen were incorporated into the structure. The determination of H2O2 was conducted without the use of enzymes with the S,N co-doped GO electrode, which exhibits high stability and does not necessitate an additional purification and separation step. On the basis of obtained results, the S, N-codoped graphene oxide electrode exhibited good performance such as satisfying accuracy for H2O2 detection in the pharmaceutical sample with excellent reproducibility and stability
The role of intraoperative consultation in the management of ovarian masses and endometrial carcinomas: a 7-year experience
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Intraoperative consultation (IC) is often used for surgical planning in gynecological
oncology, primarily in ovarian and endometrial cancer surgery. However, there is
limited research that compares IC diagnoses and results from definitive analyses. This
retrospective study compares IC results and definitive analysis findings in terms of
ovarian mass diagnoses and endometrial carcinoma patients consulted for myometrial
invasion (MI) depth across 7 years (2012–2019). IC was performed in 282 cases to
evaluate ovarian masses. The sensitivity of IC was 94% for benign ovarian masses
and 90% for malignant ovarian masses. 92 cases were submitted to IC for endometrial
carcinoma. Sensitivity was 80% for tumors with <50% MI and 74% for tumors with
≥50% MI. IC is an important method with high sensitivity and specificity for diagnosing
ovarian masses and determining MI depth in endometrial carcinomas
Positional accuracy of a single implant analog in additively manufactured casts in biobased model resin
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Objectives: To evaluate the positional accuracy of implant analogs in biobased model resin by comparing them to
that of implant analogs in model resin casts and conventional analogs in dental stone casts.
Methods: Polyvinylsiloxane impressions of a partially edentulous mandibular model with a single implant were
made and poured in type IV dental stone. The same model was also digitized with an intraoral scanner and
additively manufactured implant casts were fabricated in biobased model resin (FotoDent biobased model) and
model resin (FotoDent model 2 beige-opaque) (n = 8). All casts and the model were digitized with a laboratory
scanner, and the scan files were imported into a 3-dimensional analysis software (Geomagic Control X). The
linear deviations of 2 standardized points on the scan body used during digitization were automatically calculated on x-, y-, and z-axes. Average deviations were used to define precision, and 1-way analysis of variance and
Tukey HSD tests were used for statistical analyses (α = 0.05).
Results: Biobased model resin led to higher deviations than dental stone (all axes, P ≤ 0.031) and model resin (yaxis, P = 0.015). Biobased model resin resulted in the lowest precision of implant analog position (P ≤ 0.049).
The difference in the positional accuracy of implant analogs of model resin and stone casts was nonsignificant (P
≥ 0.196).
Conclusions: Implant analogs in biobased model resin casts mostly had lower positional accuracy, whereas those
in model resin and stone casts had similar positional accuracy. Regardless of the material, analogs deviated more
towards mesial, while buccal deviations in additively manufactured casts and lingual deviations in stone casts
were more prominent
Investigation of the effect of NF-κB-related Covid-19 pathogenesis on disease severity
No full textCOVID-19, SARS-CoV-2 virüsü tarafından tetiklenen solunum yolu hastalığıdır. Hastalığın patogenezinde, SARS-CoV-2'ye ait proteinler, NF-κB sinyal yolağının aktifleşmesine neden olan ateşleyici sinyal görevi görmektedir. NF-κB ilişkili COVID-19 patogenezinin hastalık şiddetine olan etkisini incelediğimiz çalışmadaki amacımız, NF-κB sinyal yolunun, SARS-CoV-2 enfeksiyonuna bağlı bağışıklık yanıtta nasıl bir rol oynadığını ve ACE2, CD147, TLR2, TLR4, miRNA'lar ve şimdiye kadar yapılan çalışmalarda öne çıkan sitokinler (IL-1B, IL-2, IL-6, IL-8, IL-10 ve IL-17) ile olan etkileşimlerinin COVID-19 hastalığının patogenezinde ve klinik seyirdeki rolünü detaylı bir şekilde değerlendirmektir. Çalışmaya, COVID-19 PCR testi pozitif ve asemptomatik, semptomatik ve yoğun bakım desteği gören olmak üzere üç gruba ayrılmış hastalar dahil edilmiştir. Hastalardan toplanan serum örneklerinde NF-κB, IL-1, IL-2, IL-6, IL-8, IL-10 ve IL-17F aktivitesi ELISA yöntemiyle ölçüldü. Aynı hasta gruplarından toplanan kan örneklerinde ise, NF-κB, ACE2, CD147, TLR2, TLR4, miR122 ve miR146a genlerinin ekspresyon düzeyleri gerçek zamanlı polimeraz zincir tepkimesi yöntemi ile belirlendi. Yoğun bakım hastalarında TLR4 ekspresyon düzeyi medyanının, asemptomatik ve semptomatik gruplara kıyasla anlamlı düzeyde düşüş (p= 0.0241); semptomatik grupta NF-κB ekspresyon düzeyleri ile ACE2, CD147, TLR4 ekspresyon düzeyleri arasında anlamlı pozitif ilişkiler (sırasıyla, p<0,0001, p= 0.038 ve p=0.026) ve miR-146a ve miR-122 ekspresyon düzeyleri arasında anlamlı negatif ilişkiler (p<0,0001); yoğun bakım grubunda NF-κB ekspresyon düzeyleri ile ACE2 ve CD147 ekspresyon düzeyleri arasında anlamlı pozitif ilişkiler (p<0,05) ve miR-146a ve miR-122 ekspresyon düzeyleri arasında anlamlı negatif ilişkiler tespit edildi (P<0,0001). Semptomatik grupta NF-κB ekspresyon düzeyi ile IL-1β ve NF-κB serum konsantrasyonları arasında (sırasıyla p= 0.04 ve p<0,0001); yoğun bakım grubunda ise NF-κB ekspresyon düzeyleri ile IL-1β ve NF-κB konsantrasyonları arasında (sırasıyla, p=0,044 ve p<0,0001) ve IL-2, IL-6, IL-8 ve IL-10 serum konsantrasyonları arasında anlamlı pozitif ilişkiler tespit edildi (p<0,05). Elde ettiğimiz veriler, NF-κB'nin dahil olduğu inflamatuvar sürecin COVID-19'un patogenezinde kritik bir rol oynadığını göstermektedir. Buna göre, NF-κB, COVID-19 patogenezi sırasında diğer moleküllerle etkileşime girerek immün sistemi düzenlemektedir.COVID-19 is a respiratory illness triggered by the SARS-CoV-2 virus. SARS-CoV-2 proteins act as inflammatory signals, triggering the activation of the NF-κB signaling pathway, a key regulator of inflammatory responses. This study delves into the intricate relationship between the NF-κB signaling pathway and COVID-19 pathogenesis. Our primary objective is to comprehensively examine the role of NF-κB in the immune response to SARS-CoV-2 infection and its interactions with ACE2, CD147, TLR2, TLR4, miRNAs, and prominent cytokines (IL-1β, IL-2, IL-6, IL-8, IL-10, and IL-17) identified in previous studies. The study included patients divided into three groups: COVID-19 PCR test positive and asymptomatic, symptomatic, and requiring intensive care unit support. The activity of NF-κB, IL-1b, IL-2, IL-6, IL-8, IL-10, and IL-17F was measured in serum samples collected from the patients using the ELISA method. The expression levels of the NF-κB, ACE2, CD147, TLR2, TLR4, miR122, and miR146a genes were determined in blood samples collected from the same patient groups using the real-time polymerase chain reaction method. The median expression level of TLR4 was found to be significantly lower in intensive care unit patients compared to the asymptomatic and symptomatic groups (p=0.0241). When the correlations between NF-κB expression level and other mRNA levels were examined, significant positive relationships were found between NF-κB expression level and CD147 and TLR2 expression level in asymptomatic patients (p<0.05). In the symptomatic group, significant positive relationships were found between patients' NF-κB expression level and ACE2, CD147, and TLR4 expression levels (p<0.0001, p=0.038, and p=0.026, respectively), and significant negative relationships were found between NF-κB expression level and miR-146a and miR-122 expression levels (p<0.0001). In the intensive care unit group, significant positive relationships were also found between NF-κB expression level and ACE2 and CD147 expression levels (p<0.05), and significant negative relationships were found between NF-κB expression level and miR-146a and miR-122 expression levels (p<0.0001). In the symptomatic patient group, significant positive relationships were found between NF-κB expression level and IL-1β and NF-κB serum concentrations (p=0.04 and p<0.0001, respectively). Significant positive relationships were also found between NF-κB expression levels and IL-1β and NF-κB concentrations in intensive care unit patients (p=0.044 and p<0.0001, respectively). Significant positive relationships were also found between NF-κB expression levels and IL-2, IL-6, IL-8, and IL-10 serum concentrations in intensive care unit patients (p<0.05). The findings of this study indicate that NF-κB plays a critical role in the pathogenesis of COVID-19. NF-κB seems to regulate the immune system by interacting with other molecules during COVID-19 pathogenesis
Heat transport analysis of three-dimensional magnetohydrodynamics nanofluid flow through an extending sheet with thermal radiation and heat source/sink
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Regarding heat transformation efficiency, the hybrid nanofluid performs superior to the nanofluid. The majority
of hybrid nanofluid uses are in the industrial sector, producing solar energy, cooling generators, and vehicle heat
transformation. Heat transfer and nanofluid velocity are the two most crucial transport properties that must be
evaluated before the first and second thermodynamics equations are applied to nanoscale fluids. The objective of
this work is to investigate the characteristics of transmission of heat of magnetohydrodynamic (MHD) nanofluid
(Ag/H2O)and hybrid nanofluid (Ag + Al2O3/H2O)flow on a linear extensible sheet when magnetic forces are
present. Similarity variables are applied to transform a set of nonlinear dimensionless partial-differential
equations to collection of ordinary-differential equations. The non-analytical solutions of these transformed
equations are found utilizing the MATLAB mathematical program’s bvp4c function. The impression of various
physical attributes along skin friction coefficients and properties of heat transmission are analyzed. The behavior
of key parameters, including surface stretching ratio, rotational and magnetic effects, for temperature and velocity, is shown using graphs and tables. In conclusion, hybrid nanofluids, which comprise silver and aluminum
oxide nanoparticles dispersed in water, outperform silver-water nanofluids by around 10–15 % under magnetohydrodynamic (MHD). The higher thermal conductivity of these hybrid nanofluids allows for better heat
dissipation, making them an appealing option for applications that need optimal thermal management in the
presence of magnetic field
The impact of calcium-binding protein S100P on temozolomide sensitivity in glioblastoma cells
No full textABSTRACT
Aim: Glioblastoma (GBM), a highly aggressive primary brain tumor, often displays resistance to the standard
chemotherapy agent Temozolomide (TMZ), presenting a formidable treatment challenge. This study aims to
explore the impact of S100P, a calcium-binding protein linked to TMZ resistance in GBM cells, by investigating
the consequences of S100P knockdown on TMZ resistance. The research, employing bioinformatics analysis and
siRNA-mediated gene silencing, reveals a significant relationship between S100P and TRIM29 in the context of
TMZ resistance.
Material and methods: Human GBM cells underwent transfection with S100P siRNA to achieve targeted suppression of S100P expression. The assessment of TMZ resistance was conducted through MTT cell viability assays, and the molecular mechanisms underlying the observed effects of S100P knockdown on TMZ resistance
were explored using real-time PCR.
Results: Our findings reveal a significant reduction in TMZ resistance upon S100P knockdown in GBM cells. This
effect is demonstrated by an increase in cell death and a concurrent decrease in the expression of proteins
associated with TMZ resistance.
Conclusion: The study establishes S100P as a promising therapeutic target to overcome TMZ resistance in GBM.
Silencing S100P expression emerges as a strategy to enhance the sensitivity of GBM cells to TMZ chemotherapy.
These results advocate further investigations to unveil the clinical viability of targeting S100P in the treatment of
GBM
Türkiye's 3-year coronavirus disease 2019 publication performance in the shadow of the pandemic: a bibliometric analysis
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OBJECTIVE: The pandemic created by coronavirus disease 2019 (COVID-19) has led researchers to study to deal with this infection. The number of studies about it being performed and published is increasing over time. Due to the dramatic growth of COVID-19 scientific publications, we conducted a bibliometric and visualized study to better understand the progress, collaborations, and trend topics of COVID-19 publications in T & uuml;rkiye. MATERIAL AND METHODS: The scientific literature on COVID-19 research in T & uuml;rkiye from 2020 to 2022 was extracted from the Scopus database and analyzed using bibliometric and scientometric techniques. RESULTS: A total of 426.662 COVID-19-related publications were published worldwide in the 3-year period and T & uuml;rkiye ranked 12th according to both publication (8.394) and case numbers (17.004.677). The most cooperations of T & uuml;rkiye were with the USA, the UK, and Italy. The 5 most used keywords were determined as "COVID-19," "SARS-CoV-2," "Pandemics," "Coronavirus," and "Anxiety" respectively. It was determined by the keyword analysis that the most focused topics were related to the "COVID-19 clinic." CONCLUSION: T & uuml;rkiye did not lag behind in the number of studies on her geography, and it was seen to be in the leading position compared to the Middle East countries. It is important to periodically evaluate such a large number of research articles from a broad perspective for planning and direction of future studies