Biruni University Institutional Repository
Not a member yet
2908 research outputs found
Sort by
Fas and microRNAs variations as a possible risk for Behçet disease
Full text linkBackground: Behçet disease (BD) belongs to a disease family that has a transparent borderline between autoinflammatory and autoimmune disorders. Fas and some miRNAs have revealed to display remarkable roles in both autoimmune and autoinflammatory processes, and they can play important roles in defective apoptosis in BD. We investigated the association of the susceptibility of BD with Fas, miRNA variations, and their both single and combined presence in a Turkish population as a case-control study. Methods: The distributions of FAS-670 A>G rs1800682, mir146a rs2910164, and mir196a rs11614913 polymorphisms are analyzed with the polymerase chain reaction–restriction fragment length polymorphism method in 115 BD patients and 220 controls in 6-month period. Results: Statistical analysis indicates that in the case of Fas-670 A/G rs1800682, AA genotype and A allele have a protective role in BD ( p = 0.0004 and p = 0.0009, respectively). The dominant model (AA + AG/ GG) also displays a protective effect on BD unlike the recessive model ( p = 0.03). In addition, both homozygous genotype (CC) of rs2910164 of mir-146a ( p = 0.04) and the dominant model (CC + CG vs. GG) have protective effects on BD unlike the recessive model ( p 0.05). Conclusions: These findings indicate that both Fas rs1800682 and mir146a rs2910164 variants might be important factors participating in the protection against BD in the Turkish population
A glance at the 2016 guidelines for stem cell research and clinical translation by international society for stem cell research (ISSRC)
No full textUluslararası Kök Hücre Araştırma Derneği (ISSCR) kök hücre dünyasının gelişen ve değişen yönlerini göz önüne alarak 2006 yılındaki İnsan Embriyonik Kök Hücre Araştırmalarının Yürütme İlkeleri ve 2008’deki Kök Hücrelerin Kliniğe Uyarlanması Kılavuzu’ndan sonra 2016 yılında Kök Hücre Araştırma ve Kliniğe Uyarlama Kılavuzu’nu yayınlamıştır. Bu kılavuz kök hücre alanında var olan etik, sosyal ve politik sorunları belirtirken kök hücre alanındaki temel ve kliniğe uyarlanan çalışmaların birbiriyle uyumlu ilerlemesi için öneriler sunmaktadır. Klinik öncesi ve klinik uygulamaların her alanında titizlik, gözetim ve şeffaflığın olması gerektiği vurgulanmaktadır. Söz konusu kılavuz araştırmacılara, klinisyenlere, hastalara, tedarikçilere, fon sağlayıcılara, yasa yapıcılara ve araştırmaların tüm süreçlerinde gözetimci ve denetimci olarak yer alan uzmanlara ve sonuç olarak kamuoyuna konunun kapsamını, işleyişini ve hedeflerini aktarmak amacıyla hazırlanmıştır. Bu derlemenin amacıysa ISSCR’ın 2016 kılavuzundaki öne çıkan temel öğeleri ve önerileri vurgulamak; özellikle ülkemizdeki kök hücre araştırmalarında sıklıkla ihtiyaç duyulan ve üzerinde görüş birliği sağlanmaya çalışılan noktalar için bir özet bilgi vermek ve uluslararası tanınmışlığı üst düzeyde olan ISSCR’ın konuya bakış açısını kendi dilimizde aktarmaktır.The International Stem Cell Research Association (ISSCR) takes into account the evolving and changing aspects of the stem cell world; therefore following the Guidelines for the Conduct of Human Embryonic Stem Cell Research, 2006 and the Guidelines for the Clinical Translation of Stem Cells, 2008, the task force has prepared and published most recent guidelines in 2016. The recent guideline presents suggestions for harmonious progression of studies that are based on the basic and clinical aspects of the stem cell field, while addressing the ethical, social and political problems that exist in this field. It is emphasized that there should be rigor, surveillance and transparency in all fields of preclinical and clinical applications. The guideline is intended to convey the scope, functioning and objectives of the subject, to the researchers, clinicians, patients, suppliers, funders, legislators and experts including supervisors and reviewers who are involved in all processes of stem cell research and, consequently, the public. Here, we aim to highlight the key elements and recommendations outlined in the ISSCR 2016 guidelines; to provide an overview of the points that are often needed in the stem cell research and clinical medicine in order to provide a consensus on those issues, raised by the internationally-recognized ISSCR in our own language
Predictive clinical factors of chronic peripheral neuropathy induced by oxaliplatin
Full text linkPurpose We aimed to identify potential clinical parameters that can be easily obtained by a pre-treatment clinicopathological evaluation and whole blood test to estimate the development of oxaliplatin-induced peripheral neuropathy (OIPN). Methods This study was conducted retrospectively. For the FOLFOX regimen, patients received oxaliplatin, 85 mg/m2 , every 2 weeks for 12 courses, and with the XELOX regimen, oxaliplatin was 130 mg/m2 , every 3 weeks for 6–8 courses. The incidence and degree of neuropathy (NCI-CTCAE v.3) were recorded. Results A total of 186 patients were included in the study. There were 108 (58%) patients in the grade 0–1 (G0–G1) neuropathy group (mean age 50.5 ± 11.5; 63% men), and 78 (42%) patients in the grade 2–3 (G2–G3) neuropathy group (mean age 58.0 ± 10.8; 46.2% men). The relationship between G2–G3 OIPN development and age (p < 0.001), gender (p = 0.02), and ECOG performance status (p = 0.007) was statistically significant. In the G2–G3 neuropathy group, serum gamma-glutamyl transferase (GGT) (p < 0.001) and glucose (p = 0.007) levels were higher, whereas vitamin D (p < 0.001), hemoglobin (Hgb) (p < 0.001), serum albumin (p = 0.001), and serum magnesium (p = 0.035) levels were lower compared with the G0–G1 neuropathy group. G2–G3 neuropathy was observed in 88% of patients with mucinous carcinoma pathologic type (p < 0.001). Conclusion This study demonstrated that age, histopathologic type, albumin, GGT, glucose, vitamin D, and Hgb levels were the effective factors in prediction of the development of OIPN. In addition, GGT, vitamin D, and Hgb levels were the most effective factor to predict development of OIPN
The association between SYNTAX Score II and carotid artery disease severity in patients who underwent coronary artery bypass grafting
Full text linkThe SYNTAX score (SS) and SS II, which include additional clinical parameters, are widely used today for deciding revascularization following coronary angiography. We investigated the association between the presence and severity of carotid artery disease (CrAD) using the SS and SS II in 287 patients who underwent coronary artery bypass grafting. We based this investigation on the known association between coronary artery disease and CrAD. A significant association was observed between the groups with and without CrAD in terms of SS II values (28.4 + 9.6 vs 21.4 + 7.7, respectively; P < .001). A significant difference was also observed when stenosis was classified according to severity as 70% (P < .001). The results indicated a positive correlation between the presence and severity of CrAD as SS II increased (r ¼ 0.187, P ¼ .005). According to the results of multivariate logistic regression analysis, the SS II was an independent predictor of CrAD
Inflammatory markers of contrast - induced nephropathy in patients with acute coronary syndrome
Full text linkObjective: Contrast-induced nephropathy (CIN) is among the serious complications of invasive cardiovascular procedures that are performed with the administration of contrast agents. We investigated the role of the inflammatory markers in predicting CIN in acute coronary syndrome patients. Methods: This study included 232 consecutive patients with acute coronary syndrome who underwent emergency angiography at our center. Results: There were 38 (19.1%) patients in the CIN group (mean age: 62.4 ± 10.2; 68.4% male), and 162 patients in the non-CIN group (mean age: 62.1 ± 11.5; 60.5% male). In the CIN positive group, serum gammaglutamyl transferase (GGT) (P < 0.001), uric acid (P < 0.001), high sensitivity C-reactive protein (P < 0.001), the neutrophil-to-lymphocyte ratio (P = 0.02) were higher, whereas vitamin D (P < 0.001), hemoglobin (P < 0.001) and baseline glomerular filtration rate (P = 0.011) were lower compared with the CIN negative group. The receiver operating characteristic analysis showed that the cutoff point of GGT was 56 U/L for predicting CIN with a 84.2% sensitivity and a 72.2% specificity (area under the curve = 0.879, P < 0.001). The predictive value of GGT was the highest compared other inflammatory markers for CIN (area under the curve = 0.879). Conclusion: Our study showed that the levels of GGT, high sensitivity C-reactive protein, vitamin D, uric acid and neutrophil-to-lymphocyte ratio were the effective factors in development of CIN. The level of GGT was found as the most effective factor in prediction of the development of CIN
Metformin reduces the extent of varicocele-ınduced damage in testicular tissue
Full text linkVaricocele is a medical condition where retrograde flow of blood leads to increased hydrostatic pressure in testicular veins and the prevalence of varicocele is predicted to vary between 30 to 40% in infertile men. Metformin, major therapeutic agent in the treatment of type 2 diabetes mellitus, was shown to reduce oxidative stress and apoptosis in renal tubular epithelial cells and testis of animal models. However potential protective effects of metformin against varicocele-induced testicular damage has not been studied. We investigated the impact of metformin on spermatogenesis assessed with Johnsen score, seminiferous tubule integrity and apoptotic activity assessed with the expression of cleaved caspase 3 activity. A total of 36 male Wistar rats (6-week-old) were divided into six groups (n=6 for each group); (C) control group, S (sham group), V (varicocele-only group), V+M (varicocele + metformin group), V/E (varicocele + varicocelectomy group), V/E+M (varicocele + varicocelectomy + metformin group). Metformin administration improved spermatogenesis, seminiferous tubule integrity and reduced apoptotic activity as manifested by the decreased expression of cleaved caspase 3 in rats with varicocele. However, metformin did not exhibit any additional benefit on these parameters in varicocelectomies rats. As conclusion, metformin treatment reduced the extent of damage to spermatogenesis in rats with varicocele, although no additional benefit was detected when administered following varicocele surger
An open radical prostatectomy approach that mimics the technique of robot-assisted prostatectomy: A comparison of perioperative outcomes
Full text linkPurpose: To report on an ascending radical retropubic prostatectomy (RRP) technique and determine whether this technique has better perioperative, oncological and functional outcomes than the standard RRP technique applied in our clinic. Materials and Methods: The perioperative and functional outcomes of the 246 patients that underwent standard RRP (N = 150) or modified RRP (N = 96) were evaluated, retrospectively. In the modified RRP technique the dorsal vascular complex (DVC) was controlled at first. Thereafter, the bladder neck was incised at the prostate-ves- ical junction. After seminal vesicles and vasa were exposed, posterior dissection was continued until to the apex. Finally, the urethra was divided. Results: The mean volume of estimated blood loss (EBL) was significantly longer in the standard RRP group than in the modified RRP group (610 vs. 210 ml, respectively; P = .001). The mean operative time (OT) was signifi- cantly less in the modified RRP group (177 vs. 134 min, respectively; P = .003), as were the transfusion rate TR (P = .041). With regard to the rate of postoperative complications, a statistically significant difference was observed between the two groups (P = .014). Continence rates after 3 and 12 months postoperatively were 98.95% and 98.95 % in the modified RRP group, and 97.33% and 98.66% in the standard RRP group, respectively ( P = .83). Conclusion: We observed that the EBL, TR and OT were significantly lower when we applied the modified RRP technique to patients. This modified technique might be applicable for institutions as an alternative procedure for the standard RRP technique
The anti - inflammatory effects of anacardic acid on a tnf - α - induced human saphenous vein endothelial cell culture model
Full text linkAbstract: Background and Objective: Coronary bypass operations are commonly performed for the treatment of ischemic heart diseases. Coronary artery bypass surgery with autologous human saphenous vein maintains its importance as a commonly used therapy for advanced atherosclerosis. Vascular inflammation-related intimal hyperplasia and atherosclerotic progress have major roles in the pathogenesis of saphenous vein graft disease. Methods: In our study, we investigated the effect of anacardic acid (AA), which is a bioactive phytochemical in the shell of Anacardium occidentale, on atherosclerosis considering its inhibitory effect on NF-κB. We observed relative ICAM-1 and NF-κB mRNA levels by qRT-PCR method in a TNF-αinduced inflammation model of saphenous vein endothelial cell culture after 0.1, 0.5, 1 and 5 µM of AA were applied to the cells. In addition, protein levels of ICAM-1 and NF-κB were evaluated by immunofluorescent staining. The results were compared between different concentrations of AA, and also with the control group. Results: It was found that 5 µM, 1 µM and 0.5 µM of AA had toxic effects, while cytotoxicity decreased when 0.1 µM of AA was applied both alone and with TNF-α. When AA was applied with TNF-α, there was a decrease and suppression in NF-κB expression compared with the TNF-α group. TNF-α-induced ICAM-1 expression was significantly reduced more in the AA-applied group than in the TNF-α group. Conclusion: In accordance with our results, it can be said that AA has a protective role in the pathogenesis of atherosclerosis and hence in saphenous vein graft disease