Biotropic : The Journal of Tropical Biology
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Dogs (Canis lupus familiaris) as Virus Carrier in Indonesia
Get PDFA Virus is an individual that cannot be described as an animal or a plant. If animals and plants contain two nucleic acids (DNA and RNA), on the contrary, the virus only has one of them. These nucleic acids can stimulate a complete virus replication cycle. The virus can only replicate and live on a living host if the host is finally dead, then the virus will move on the cells that are still alive. The virus has genetic material which is a protective protein coat called a capsid. Viruses can infect various varieties of organisms, both eukaryotes (animals, plants, protists, and fungi) and prokaryotes (bacteria and archaea). The Virus infects bacteria known as bacteriophage (phage). The Virus can cause serious diseases for humans such as AIDS, HIV, rabies, etc. Dogs belonging to Canidae family are the sibling of wolves, foxes and raccoon dogs. Among all members of Canidae, dogs have the most closely related to wolves which are the ancestors of dogs. The Canidae family generally has a small elongated body, sharp ear and muzzle, sharp smelling, can run fast and can swim. Dogs are human best friends. While taking care of the dogs, they can be attacked by various diseases. The closeness of the relationship between humans and dogs raises the potential for disease transmission, especially zoonosis and pandemics viruses.
Keywords: Canis lupus familiaris, Carier, Influenza, viru
Phenotipical Characters of Melon (Cucumis melo L.) in Response to Karst Critical Land
Get PDFYogyakarta Agroecosystem has 158,600 ha of critical land spread over three zones. Two areas are Karst Land, located on Agroecosystem II includes Gunungsewu Hills, Gunungkidul and III covers Dlingo Bantul Hills and Sentolo Hills Kulon Progo Regency.. Karst Land is certainly provides stress to plants. These research purposes are examining the phenotype character of superior melon Cultivar TACAPA compare to parents and offsprings phenotypes. The phenotype characters are based on plant height, leaf number, time of melon flowering, water content of plants, and fruit and seed productivity. This experiment was done by Split Plot Design with Completely Randomized Design (CRD) with 4 kinds of treatment (control plant media, Gunungsewu, Dlingo, and Sentolo), 7 experimental units cultivars: TACAPA, TA, TP, PT, AT, Action 434, PI 371795), and 4 replications. Research result reveals that most of the phenotypic characters including plant height, number of leaves, fruit weight, and number of seeds produced have relatively no significant effect between treatment and control, while the phenotypic first time flowering time and water content of the plant, have a noticeable difference
Pengaruh Latihan Renang Teratur dan Latihan Renang Tidak Teratur Terhadap Kadar Trigliserida Plasma pada Rattus norvegicus
Get PDFTriglycerides are source of energy in the body, but triglyceride deposits that exceed the threshold will cause health problems, so triglycerides should be lowered to normal. The reduction of triglycerides can be done through physical activity, therefore this study aims to reveal the effect of regular swimming exercises and irregular exercises against triglyceride levels. This research is an experimental research using The Post Test Only Control Group Design design. Subjects of the study were male of Rattus Norvegicus Wistar, 39 subjects was divided into 3 groups with Matched Subject Design technique, which consisted of 2 groups of treatment that is regular and irregular group, and control group. The exercise program was given a swimming with a 3% weight of body weight and was tied 5 cm from the tip of the tail which was performed 3 times per week consistently for regular exercise and 0 to 5 times per week inconsistently for irregular exercise for 12 weeks, While the control group was not given training. In the last week before the blood was taken, the first three groups were given swimming treatment exercises. Based on the results of the anava test, there was a significant difference in the reduction of triglyceride levels among white mice who were given regular exercise with irregular exercise. These results suggest that regular exercise has a better impact on triglyceride reduction than irregular exercise, due to the higher adaptation process of regular exercise
Isolasi Bakteri Selulolitik dari Limbah Kulit Pisang
Get PDFCellulose can be found in whole parts of plants. Cellulose can be degraded by cellulose enzyme. Cellulose enzyme is produced by cellulolytic bacteria which has ability to degrade the cellulose and fiber. Cellulose enzyme is extremely important in industry sector. This research aims to isolate the cellulosic bacteria of peel waste of Banana in Sorong, West Papua. This research used isolation and purification method by using 1 gram of Banana peel. It was isolated in CMC (Carboxymethyl cellulose) selective media and morphology characteristic test using 400X-1000X microscope and zone index test. The result showed that the cellulosic ability was found in isolate B with pure zone index 3mm and the smallest pure zone index was in isolate H with pure zone index 0,8 mm. while, the isolate C had zone pure index 1,5 mm, isolate D 1,625 mm, isolate E 1 mm, isolate F 0,714mm, and isolate G 2 mm. 
Potency of Curculigo capitulata and Curculigo latifolia Fruit Based on Nutrient Content (a Case for Preservation Need in Kebonharjo)
Get PDFC.capitulata and C.latofilia are recognized as a medical plants in Asia, especially for ancient India and China. In Indonesia, i.e Kebonharjo (a village in Samigaluh District, Kulon Progo, Yogyakarta), people use it as a sweetener replacing sugar. Phenol, neonorlignan, and protein inside these plants had been examined by some researchers. However, the nutrient content inside the fruit are rare or slight recorded. This study aims to reveal the composition of minerals (Ca, Fe, Mg, K), carbohydrate compound (glucose, fructose, sucrose) and Vitamin C in their fruit, therefore, the development to preserve these species can be concerned due to the plan of “Bedah Menoreh” program, a program of road construction connecting between New Yogyakarta International Airport (NYIA) and Borobudur Temple. The method for finding out the nutrient content is by laboratorium analysis. Result showed that both fruit contained potentially high of mineral, especially Ca (calcium), but C.capitulata and C.latifolia contained no sucrose and glucose respectively
Comparison of DNA Isolation Results with Simple Methods and Kits in Samples of Psidium guajava Leaves
Get PDFDNA isolation is one of a series of methods that must be carried out on the basic techniques of Molecular Biology Analysis. Especially PCR-based molecular marking techniques. Many ways are done in DNA isolation. This study discusses the comparison of the results of DNA isolation using two methods. Simple DNA isolation methods and using Kit. Samples of Psidium guajava leaves used were taken from 15 different locations used. In general, DNA isolation methods include three steps, namely destruction, precipitation, and purification. Simple DNA isolation is done with detergents, alcohol groups, which are commonly available in the laboratory. Methods of DNA isolation with KIT are carried out according to the Promega Universal Wizard KIT protocol. The comparison results are seen from spectrophotometric absorption Å230 nm, Å260 nm, Å280 nm, Å320 nm, ratio Å260/Å230, ratio Å260/Å280 to see DNA purity, protein concentration before purification step, and DNA concentration produced. The results showed that there were no statistically significant differences in the results of DNA isolate spectrophotometry. However, the use of KIT with modified protocols is more recommended if researchers want to carry out DNA analysis more precisely and accurately.
Keywords: DNA isolation, spectrophotometry, DNA concentration and purit
Diversitas Arthropoda Gua di kawasan Karst Gunung Sewu, Studi gua-gua di Kabupaten Wonogiri
Get PDFPenelitian ini bertujuan untuk mengungkap keanekragaman Arthropoda di kawasankarst Gunung Sewu khususnya di wilayah kabupaten Wonogiri. Penelitian ini merupakanpenelitian ekologi komunitas dengan metode Nature Snapshot Experiment (NSE). Penelitian inidilaksanakan pada bulan Agustus 2016, bertempat di 3 gua di kawasan karst Gunung Sewu,yang masuk dalam wilayah administrasi Kabupaten Wonoggiri Jawa Tengah. Arthropoda guayang ditemukan terdiri dari Heteropoda sp., Charon grayi, Theliphonus sp., AsamiidaeF, Philosciasp.,CambalopsidaeF, Geophilus sp., Scutigera sp., TenebrionidaeF, Rhaphidophora dammermani,dan FormicidaeF. Habitat gua Sodong dan Potro Bunder membentuk kelompok tersendiriberdasarkan nilai indeks similaritas Jaccard. Kondisi kerusakan lingkungan gua mendasaripemilihan habitat oleh kelelawar. Sehingga dua gua yang mengelompok hanya dihuni lebihsedikit spesies dibandingkan dengan habitat gua Song Gilap.Kata Kunci: Diversitas, Arthropoda, Gua, Karst Gunung Sew
Perilaku Reptilia Ketika Gerhana Matahari Parsial di PASTY Yogyakarta
Get PDFPerilaku Reptilia di Pasar Aneka Satwa & Tanaman Hias Yogyakarta diamati ketika peristiwa gerhana matahari parsial pada 9 Maret 2016. Faktor lingkungan seperti intensitas cahaya, suhu, dan kelembaban udara diamati dan dicatat. Perilaku Kura-Kura, Biawak, dan Ular diamati dengan metode focal point sampling. Ular cenderung memiliki pergerakan yang terbatas, sedangkan biawak dan kura-kura diam sejenak. Perbedaan perilaku reptilia ketika gerhana matahari sebagian ditentukan oleh jenisnya dan faktor lain yang berbeda-beda
Studi Pencemaran Kandungan Logam Berat Timbal (Pb) dan Kadmium (Cd) pada Ikan Tongkol (Euthynnus sp.) di Pantai Utara Jawa
Get PDFoai:oai.jurnalsaintek.uinsby.ac.id:article/132Logam berat dalam lingkungan perairan akibat pembuangan limbah industri di sepanjang wilayah pantai utara dapat menjadi sumber racun bagi kehidupan perairan. Berdasarkan data penelitian dari Badan Penelitian dan Pengembangan Provinsi Jawa Tengah diketahui adanya kandungan logam berat di perairan Laut Jawa, memungkinkan adanya kotaminasi pada ikan laut hasil Laut Jawa yang didistribusikan ke masyarakat. Hal ini menyimpang dari peraturan pemerintah yang mengatur dan melindungi keamanan pangan yaitu PP Nomor 28 tahun 2004 tentang Keamanan, Mutu, dan Gizi Pangan. Penelitian ini bertujuan untuk mengetahui dan mengukur kadar logam berat Pb dan Cd pada ikan tongkol di Pantai Utara Jawa. Penelitian ini bersifat deskriptif dengan pendekatan cross sectional study. Populasi dalam penelitian ini adalah seluruh ikan tongkol (Euthynnus sp). Sampel penelitian ini diambil secara purposive sampling yaitu 10 ekor ikan tongkol yang berasal dari daerah Kendal, Rembang, Tuban, Batang, dan Jepara. Hasil penelitian ini berdasarkan Peraturan BPOM tahun 2009 dan SNI 7387 tahun 2009 tentang Batas Maksimum Cemaran Logam Berat pada Makanan, dari 10 sampel terdapat 4 sampel (40%) melebihi batas maksimum cemaran Pb pada ikan (< 0,3 mg/kg) dengan kadar antara 0,420-0,610 mg/kg dan 6 sampel (60%) melebihi batas maksimum cemaran Cd pada ikan (<0,1 mg/kg) dengan kadar antara 0,100-0,300 mg/kg
CmBGI Gene Expression encoding β-glucosidase in melon (Cucumis melo L.) under stress condition
Get PDFCmBGI is the enzymatic genes encoding β-glucosidase that involved in Abscisic Acid (ABA) metabolism of Cucumis melo L. β-glucosidase promotes the accumulation of glucose, fructose, and sucrose, and it might act as a regulator that mediates melon fruit ripening both climacteric and nonclimacteric. ABA mediates adaptive responses to abiotic and biotic stresses. Agricultural Balitbang in 1997 showed that there were approximately 158.600 ha of degraded land scattered in three zones of agroecosystems in Yogyakarta (DIY). One of them is Dlingo Bantul area which has a karst type critical land area. Karst provides stress to the certain plant growth. One way to conserve critical land is making this area for agriculture. Cultivar TACAPA and TA were superior melons that have been developed by Genetic Laboratory of Biology Faculty UGM. This preliminary research was conducted to examine molecular characterization of CmBGI gene expression in cultivar TACAPA and TA which are planted in normal condition medium and in critical land medium treatment. Total RNA was extracted from leaf tissue then Reversed Transcriptase (RT-PCR) to collect cDNA library. cDNA was amplified using specific primer. Spectrophotometry was conducted in λ260 nm and electrophoresis run in 1.5% agarose gel. Control of band chosen was Cm-Actin. CmBGI gene concentration of TACAPA and TA in normal condition medium are in succession 578.5 and 579.4 μg/ml then for critical land medium treatment 743.4 and 773.5 μg/ml. CmBGI band was showed both of TACAPA and TA as ± 1258 bp. Cm-actin was showed band of DNA as ± 445 bp. CmBGI gene concentration in critical land medium treatment which is given greater stress on melons are higher than normal condition. This suggests that the CmBGI gene is expressed more in cultivar TACAPA and TA melons when they are grown under stress condition