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The Recent record of the Phalakron Plain Plushblue Flos apidanus phalakron (Lepidoptera: Lycaenidae) in South Sumatra after 91 years break
No full textAn individual of Phalakron Plain Plushblue Flos apidanus phalakron was observed and phtographed on 17 March 2020 at campus Sriwijaya University, Indralaya, South Sumatra province. In South Sumatra, this butterfly only known from a historical record in 1929. The recent observation of Flos apidanus phalakron in Indralaya represent a new record for South Sumatra province after 91 years break.Satu individu kupu-kupu dari jenis Flos apidanus subjenis phalakron teramati dan difoto pada tanggal 17 Maret 2020 di Kampus Universitas Sriwijaya, Indralaya, Sumatera Selatan. Di Sumatera Selatan, kupu-kupu ini hanya diketahui dari catatan sejarah pada tahun 1929. Pengamatan terkinan darikupu-kupu Flos apidanus phalakron di Indralaya merupakan catatan terkini untuk Sumatera Selatan sesudah 91 tahun tidak pernah dilaporkan
The Influence Of Kesambi Leaf Extract (Schleichera oleosa) On Proliferation Granulosa Cell Goat Ovarium (Caprus aegagrus HIRCUS) In Vitro
Get PDFKesambi (Schleichera oleosa) belonging to the Sapindaceae family has very important phytochemical elements including terpenoids, falvonoids, phenolic acids, betulins, betulin acids and others, so that they have enormous benefits in the process of antimicrobials, antioxidants, anticancer and can be used for the production of biodiesel. One of the potency of antioxidant ability as an antioxidant is due to its phenolic compounds. The presence of antioxidant activity in cells can affect the defense of cell protein membranes, so that the cell\u27s ability to carry out division is optimal. In vitro this is mostly done by adding hormones to culture cell media or natural compound substances to increase cell proliferation. This study is an experimental study using a Complete Random Design (CRD) with 5 treatments and 4 replications as follows: K0: granulosa cells and culture media without treatment (0 µl), P1: granulosa cells and culture media which were given 1% porch leaf extract (30 µl), P2: granulosa cells and culture media which were given kesambi leaf extract 1.5% (45 µl), P3: granulosa cells and culture media were given 2% (60 µl) kesambi leaf extract and P4: granulosa cells and culture media were given a 2.5% (75 µl) kesambi leaf extract. In this study, there was an influence on the administration of kesambi (Schleichera oleosa) leaf extract to the proliferation of goat ovarian granulosa cells in vitro in the P3 (2%) treatment ie a dose of 60 µl. However, it did not have a significant effect on the viability of goat ovarian granulosa cells
The Potention of Nipah Fiber (Nypa fruticans Wurmb.) in The Production of Nata Fruticans Using Soybean Sprouts as a Source of Nitrogen
Get PDFNypa is a mangrove plant that acts as a food source, such as nata. Research has shown that nipah plant waste, such as frond fibers and nipah fruit skin fibers, can be used as a raw material in making nata fruticans. The purpose of this study was to determine the potential of nipah fiber (Nypa fruticans Wurmb.) in nata production using soybean sprouts as a nitrogen source. The samples of nipah fiber (Nypa fruticans) used were leaves (DN), midrib (PN) and fruit skin (KB), taken in the Kuala Tungkal area, West Tanjung Jabung Regency, Jambi Province. This research method includes making the mother solution, making nata starter, and producing nata sheet by using soybean as a source of nitrogen with a concentration of 5%, 10% and 15% and ZA as a control. The results of this study indicate that the best volume and thickness of nata fruticans can be seen from the midrib substrate with a concentration of 10% soybean sprouts. The yields produced for all treatments were still lower compared to controls. Meanwhile, the water content of all treatments was better than the control. The best quality of Nata Fruticans based on thickness, yield and average moisture content was generally obtained from the substrate from the leaf midrib fibers with a concentration of 10% soybean sproutsNipah (Nypa fruticans) merupakan salah satu tumbuhan mangrove yang berperan sebagai sumber pangan dan energi. Telah diteliti bahwa limbah tanaman nipah yang khususnya serat pelepah dan serat kulit buah nipah dapat dimanfaatkan lebih lanjut sebagai bahan baku untuk pembuatan nata fruticans. Selain sebagai substrat nata fruticans, serat juga memiliki manfaat seperti mengontrol bobot badan atau obesitas, menanggulangi penyakit diabetes, mencegah gangguan gastrointestinal, mengurangi tingkat kolesterol darah dan penyakit kardiovaskuler. Penelitian ini bertujuan untuk mengetahui potensi serat nipah (Nypa fruticans Wurmb.) dalam produksi nata fruticans dan untuk mengetahui serat nipah yang manakah yang lebih bagus digunakan dalam produksi nata fruticans. Serat nipah (Nypa fruticans) yang berbeda berupa kulit buah, daun, dan pelepah diambil di daerah Kuala Tungkal Kabupaten Tanjung Jabung Barat, Provinsi Jambi. Produksi nata fruticans diberi perlakuan kacang kedelai dan menggunakan ZA sebagai kontrol. Hasil Penelitian ini menunjukkan volume nata frutican yang terbentuk dari serat nipah yang berbeda dengan perlakuan sumber nitrogen kecambah kacang kedele lebih baik dibandingkan kontrol pada konsentrasi 15% dan/atau 10%. Ketebalan nata fruticans yang terbaik adalah 6,25 mm yang terlihat pada sumber serat daun nipah dengan konsentrasi kecambah kedele 10%. Sedangkan rendemen yang terbentuk pada kontrol lebih banyak dibandingkan perlakuan yang diberikan kecambah kedele hampir pada semua sumber serat kecuali rendemen yang terbentuk dari sumber serat kulit buah
Keanekaragaman Amfibi di Air Terjun Kota Lubuklinggau, Sumatera Selatan
No full textLubuklinggau has many natural location that supports life of amphibians. Temam and Sando waterfall area were part of them. This study aims to determine diversity amphibian in the Temam and Sando Waterfall, Lubuklinggau City. The study was conducted from June to July 2020. The collection data used Visual Encounter Survey (VES) techniques and time search. he data were analyzed using relative abundance, and indexes of diversity, evenness, similarity, and dominance. 11 species of amphibians belonging to 5 families were found during the study. Odorrana hosii was found mostly during the study. Amphibian diversity in the waterfall of Lubuklinggau City was categorized as low.Lubuklinggau memiliki banyak lokasi alam yang mendukung kehidupan amfibi. Kawasan air terjun Temam dan Sando menjadi bagian darinya. Penelitian ini bertujuan untuk mengetahui keanekaragaman amfibi di Air Terjun Temam dan Sando Kota Lubuklinggau. Penelitian dilakukan dari bulan Juni sampai Juli 2020. Pengumpulan data menggunakan teknik Visual Encounter Survey (VES) dan pencarian waktu. Data dianalisis menggunakan kelimpahan relatif, dan indeks keanekaragaman, kemerataan, kesamaan, dan dominansi. Selama penelitian ditemukan 11 spesies amfibi yang termasuk dalam 5 famili. Odorrana hosii ditemukan paling banyak selama penelitian. Keanekaragaman amfibi di air terjun Kota Lubuklinggau tergolong rendah
Effect of Different Strenght of Medium on Germination and Seedling Growth of Tomato and Sucrose Effect on Biomass of Tomato Callus
No full textBioactive compounds of plant cell culture extracts are currently being looked at for its potential for the development of nutraceutical products. Tomato callus is known to have cytoprotective activity. The availability for explants and its callus production are two important things. This paper reports the effect of different strengths of medium on seed germination and seedling growth of tomato cv. Permata and sucrose effects on their biomass callus. Seeds were grown on 4 kind of germination media. Hypocotyl were taken as explants for callus induction in MS medium supplemented by 2.0 mg/l BA and 0.2 mg/l NAA, 3% sucrose, and subcultured after 20 days on medium with 6 variations of sucrose. The highest values for tomato hypocotyl’s height were obtained from the ½ and ¼ MS, while the highest fresh weight was obtained from the MS 0. Both the fresh weight and dry weight of the callus increased along with increasing sucrose concentration. The explant age and biomass need to be a concern regarding what kind of callus that will be produced. Application of 5% sucrose concentration is recommended for callus production in this study
Primers Designed For Amplifying TCF7L2 Gen
Get PDFDiabetes Mellitus (DM) is a disease characterized by high glucose levels because of the lack of insulin hormone in the body. Prevalence of Type-2 Diabetes Mellitus is associated with the variant of the TCF7L2 gene polymorphism, or SNP. The purpose of this study was to construct a specific primer to amplify the TCF7L2 gene fragment and their capabilities to detect the targeted area. This research was a descriptive study that design the primers using Geneious software version 5.4.7. The study was conducted at the Laboratory of Genetics and Biotechnology FMIPA Universitas Negeri Padang. Based on this research, a 10qF forward primer (NG_012631.1) and 10qR reverse primers (NG_012631.1) has successfully designed. The primers can amplify the targeted area of the TCF7L2 gene with 1208 bp length. The amplified gene region has many variants that are strictly related to the appearance of Type 2 Diabetes Mellitus
Formulation Of Ointment From Extract Combination Of Lemongrass (Cymbopogon nardus L. Rendle), Green Betel Leaf (Piper betle L.) And Natural Zeolite As An Antibacterial Agent
Get PDFLemongrass (Cymbopogon nardus L. Rendle) and green betel (Piper betle L.) plants are native to Indonesia. Empirically betel leaf can be used as a wound healer, lemongrass leaves are widely used as medicine for insect bites. On the basis of information obtained from various literatures, in this research has been done formulation of ointment from extract combination of lemongrass, green betel leaf and natural zeolite as antibacterial of Staphylococcus aureus. The use of zeolites based on features has a three-dimensional framework structure that is interconnected so that it has a large surface. Based on these reasons, it is believed that zeolites can increase the activity of active substances in ointments. The formulation of ointment extracts of lemongrass leaves-green betel leaves and zeolites with various dosages are KZ (zeolite control), KE (20% -5%), F1 (10% -6% + zeolite), F2 (20% - 5% + zeolite), F3 (30% -4% + zeolite), F4 (20% -0% + zeolite), F5 (0% -5% + zeolite) with a fixed amount of zeolite (2 gr). Evaluation of ointment preparations including organoleptic test, pH, homogeneity, spreading test and adhesion test showed results that met the requirements. The sample was tested for antibacterial activity with positive control (gentamicin 0.1%) showed that all formulas can provide an inhibition zone against staphylococcus aureus, except KZ. Test results were analyzed using One Way Annova with sig (0,000) <p value (0,050) followed by Tukey\u27s further tests. From this explanation it can be concluded that the formulation of ointment from extract combination of lemongrass leaves, green betel leaves and zeolites has antibacterial agent.Tanaman sereh (Cymbopogon nardus L. Rendle) dan sirih hijau (Piper betle L.) adalah tanaman asli Indonesia. Secara empiris daun sirih dapat digunakan sebagai penyembuh luka. Sedangkan daun sereh banyak digunakan sebagai obat untuk gigitan serangga. Atas dasar informasi yang didapatkan dari berbagai literatur, maka pada invensi ini peneliti membuat formulasi sediaan salep dari ekstrak daun sereh, daun sirih hijau dan zeolit. Penggunaan zeolit yang didasarkan pada keistimewaan mempunyai struktur kerangka tiga dimensi yang saling berhubungan sehingga memiliki permukaan yang luas. Berdasarkan alasan tersebut, maka zeolit diyakini dapat meningkatkan aktivitas kerja zat aktif didalam sediaan salep. Invensi ini bertujuan untuk membuat formulasi sediaan salep kombinasi ekstrak daun sereh, daun sirih hijau dan zeolit sebagai antibakteri. Invensi ini meliputi pembuatan formulasi sediaan salep ekstrak daun sereh, daun sirih hijau dan zeolit dengan variasi dosis KZ(kontrol zeolit), KE(20%-5%), F1(10%-6%+zeolit), F2(20%-5%+zeolit), F3(30%-4%+zeolit), F4(20%-0%+zeolit), F5(0%-5%+zeolit). Tahap selanjutnya sampel dilakukan uji aktivitas antibakteri terhadap bakteri Staphylococcus aureus pada sediaan salep dengan Kontrol positif (gentamicin 0,1%). Hasil uji dianalisis menggunakan One Way Annova dengan nilai sig(0,000) < nilai p (0,050) dilanjutkan dengan uji lanjut Tukey. Dari penjelasan tersebut dapat disimpulkan bahwa formulasi sediaan salep dari ekstrak daun sereh, daun sirih hijau dan zeolit memiliki aktivitas sebagai antibakteri
Variation Of Andrographolite Conten From Sambiloto (Andrographis paniculata Ness) Which Grow In Various Regions
Get PDFSambiloto is used as a hepatoprotector, enhancing the immune system, antidiabetic, anti-inflammatory, antiomalaria, antidiarrheal and many more. One of the main ingredients of the bitter plant is andrographolite. This plant is widely spread throughout Indonesia ranging from lowlands to high plains with various types of soil and altitude. The existence of this fairly wide distribution can give effect to the content of andrographolite, so that in this study will be studied about variations in andrographolite levels source from various regions. The results of this study will be used as a quality reference for treatment based on andrographolite. Samples of bitter plants are taken from various regions. Furthermore, parts of plants that are above the ground are separated between leaves, twigs and stems. All samples were dried in an oven at 60 degrees Celsius. Pollinated samples were then sieved. Samples were extracted with ethanol overnight and then analyzed their andrographolite levels with TLC-densitometry.The results of the analysis of andrographolite levels showed that bitter from various regions have different levels. Tthe organs of plants also show different levels of andrographolite as well. Part of the leaf organ is the part that contains the most andrographolite. 
Studi Lentinus squarrosulus dari Jawa Barat berdasarkan data molekuler dan morfologi
No full textLentinus is a unique genus within Polyporales, because of the lamellate basidiocarp. In Indonesia, Lentinus is commonly for a study about their potential, rarely for their taxonomy. BO 24427 specimen was found in West Java, Indonesia, sited in Landscape Arboretum of IPB University. The specimen was identified using molecular study and supported by some morphological data of fresh fruiting body. molecular identification used ITS region. The phylogenetic tree was constructed using MEGA Version X software. The morphology was based on macroscopic and microscopic observation. The result of molecular analyses showed that BO 24427 specimen was identified as Lentinus squarrosulus with 99% bootstrap value and classified into section Rigidi. The morphological data of fresh fruiting body supported the molecular identification. The important morphological data that classified into Lentinus squarrosulus were scabrous cap and size of basidiospores. This study was the first record for publish the finding of Lentinus squarrosulus in Indonesia.Lentinus adalah satu genus unik di dalam Polyporales, karena basidiocarp yang berbentuk lamella. Di Indonesia, Lentinus umumnya adalah sebuah studi mengenai potensinya, jarang sekali tentang taksonominya. Spesimen BO 24427 ditemukan di Jawa Barat, Indonseia, berlokasi di arboretum landscape Universitas IPB. Spesimen tersebut diidentifikasi menggunakan studi molekuler dan didukung oleh data morfologi dari tubuh buah yang segar. Identifikasi molekuler menggunakan daerah ITS. Pohon filogenetik dibangun menggunakan perangkat lunak MEGA versi X. Morfologi didasarkan atas pengamatan makroskopik dan mikroskopik. Hasil dari analisis molekuler menunjukkan bahwa spesimen BO 24427 teridentifikasi sebagai Lentinus squarrosulus dengan nilai Bootstrap 99% dan diklasifikasikan ke dalam bagian kelompok Rigidi. Data morfologi dari tubuh buah yang segar mendukung hasil identifikasi molekuler. Data morfologi yang penting dalam mengklasifikasikan ke dalam Lentinus squarrosulus adalah tudung yang memiliki ornamen sisik dan ukuran dari basidiospora. Studi ini merupakan catatan pertama untuk mempublikasikan penemuan Lentinus squarrosulus di Indonesia
Antibacterial Activity Test of Wet and Dried Extracts of Calabash Tree (Crescentia cujete L.) against Aeromonas hydrophilla: Natural drug use extracts of calabash tree to control the growth of Aeromonas hydrophilla by In vitro
No full textStudy to determine the antibacterial activity of wet and dry extract of the leaf, fruit, and bark of Calabash tree (Crescentia cujete L.) against the growth of Aeromonas hydrophila. The solvent extraction process was done by using 96% ethanol in the maceration method. Antibacterial test results using diffusion agar to decide clear zone and tube series of dilution test to provide MIC and MBC. Fresh leaf extract produces the highest clear zone diameter (20.06 mm), after which fresh bark extract (12.81 mm), and the last is fresh fruit extract (3.22 mm). In contrast to fresh extracts, the dried extracts are have not clear zone. MIC (Minimum Inhibitory Concentration) of Calabash Tree fresh leaf extract against Aeromonas hydrophila is 80%, and MBC (Minimum Bactericidal Concentration) is 100%