Jurnal Biota
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    Inventarisasi Siput Darat di Danau Darungan, Taman Nasional Bromo Tengger Semeru 2019

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    This study aims to determine the diversity of land snails that have never been revealed before. The location of this research is in the forest in the Ranu Darungan Resort area of Bromo Tengger Semeru National Park. The method used in this study is the 1.5 km transect line method. Land snails are often found behind leaves, around tree roots, and around leaf litter. The results of this study obtained 20 species of land snails, each of which has different characteristics. Land snail identification is using a land snail identification book. The lack of information about land snails in the Ranu Darungan Resort area makes this research expected to be the basis for further research.Penelitian ini bertujuan untuk mengetahui keanekaragaman siput tanah yang belum pernah terungkap sebelumnya. Lokasi penelitian ini adalah di hutan di kawasan Ranu Darungan Resort Taman Nasional Bromo Tengger Semeru. Metode yang digunakan dalam penelitian ini adalah metode garis transek 1,5 km. Siput darat sering ditemukan di belakang daun, di sekitar akar pohon, dan di sekitar sampah daun. Hasil penelitian ini diperoleh 20 spesies siput darat, yang masing-masing memiliki karakteristik berbeda. Identifikasi siput darat menggunakan buku identifikasi siput darat. Kurangnya informasi tentang siput tanah di kawasan Ranu Darungan Resort membuat penelitian ini diharapkan menjadi dasar untuk penelitian lebih lanjut

    The Effect Of Colchicine on Phenotypes And Stomata Pakcoy (Brassica Rapa L) Hydroponically With The NFT (Nutrient Film Technique) System

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    Colchicine (C22H25O6N) is a toxic alkaloid compound that causing a polyploidy in the plants, so it produces a robust individuals. The aims of this research are to know the effect of kolchhisin against phenotypes and stomata pakcoy (Brassica rapa L) hydroponically with the NFT (Nutrient Film Technique) system. This research used experimental method with a complete random design (CRD), five treatments and five replications.  The data were analyzed by using ANAVA with 5% of credibility. The sample of this research were used pakcoy red arrow F1 nauli with colchicine P0 (Control), P1 (250 mg / L), P2 (500 mg / L), P3 (750 mg / L), P4 (1000 mg / L). The result of this study showed that the influence of colchisin against phenotype and the stomata’s size of pakcoy plant (Brassica rapa L). The high concentrations of colchicine can caused the size of the phenotype (leaves’ number, leaf area, wet weight, dry weight) pakcoy smaller than the control. While the size of the stomata increased, the largest of stomata size was found in the P3 treatment (750 mg / L).Kolkisin (C22H25O6N) merupakan senyawa alkaloid toksik yang dapat  dapat memicu poliploidi pada tanaman sehingga menghasilkan individu jagur. Penelitian ini bertujuan untuk mengetahui pengaruh kolkhisin terhadap fenotipe dan  stomata  pakcoy (Brassica rapa L) secara  hidroponik dengan sistem  NFT (Nutrient Film Technique).  Metode penelitian ini menggunakan metode eksperimen dengan Rancangan Acak Lengkap (RAL), 5 perlakuan dan 5 ulangan. Data dianalisis menggunakan anava dengan taraf kepercayaan 5%. Sampel yang digunakan adalah pakcoy panah merah nauli F1 dengan perlakuan kolkisin P0 (Kontrol), P1 (250 mg/L), P2 (500 mg/L), P3 (750 mg/L), P4 (1000 mg/L). Hasil penelitian menunjukkan bahwa adanya pengaruh  kolkhisin terhadap fenotip dan ukuran stomata tanaman pakcoy (Brassica rapa L). Konsentrasi kolkisin yang tinggi mengakibatkan ukuran fenotipe (jumlah daun, luas daun, berat basah, berat kering) pakcoy lebih kecil dibandingkan dengan kontrolnya. Sedangkan ukuran stomata semakin bertambah, ukuran stomata yang paling besar terdapat pada perlakuan P3 (750 mg/L)

    Monitoring Monitoring Angka Coliform dan Eschercia Coli Pada Air Minum Isi Ulang Sebagai Bioindikator Pencemaran

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    One of the very needed natural resources on our planet is water. A total of 70% of the Earth\u27s surface is water utilized for human development such as drinking, irrigation and cultivating fish. Contaminated water will have a bad effect on all living creatures and their environment [7]. Sources of water pollution can be derived from manusi stool containing coliform bacteria. The research purposes will be monitoring colifom number and Escherichia coli on some samples of drinking water as a bioindicator of drinking water pollution considering the higher the population consuming drinking water refill. This resource is pure resource experiment that use design of resource descriptive analitic. The data obtained will be displayed in the frequency distribution table to see the figure of coliform and E.coli in refill drinking water. From the results of this study, of 20 samples of drinking water refill there were 14 unqualified samples where the coliform figures exceeded quality standards. And 5 positive samples of E. Coli bacteri

    Comparison Study Of Bacteria On Pangasius djambal In Ponds And River In Pagelaran Lampung

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    Pangasius djambal is one of the popular fish. The obstacle in this fish culture is the presence of bacterial attack, which is thought to originate from the waters where the fish live. This study aims to determine the differences in the number of bacteria found in the body of P djambal that are cultivated in ponds and those that live in rivers. Based on research, it is known that the number of bacteria found in the body of fish originating from rivers is more than the number of bacteria originating from ponds. The results showed the number of bacteria in the gills of fish from river reached 250 x 107 Cfu/g and in mucus 199 x 107 Cfu/g. While the number of fish bacteria originating from ponds in the gills section is 204 x 107 Cfu/g and in the mucus section is 131x107 Cfu/g

    Utilization Of Agung Semeru Banana Peel Extract As Natural Hand Sanitizer

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    Hand sanitizers generally contain Ethyl Alcohol 62%, softener, moisturizer and anti-bacterial compounds such as tryclosan, glycerol, tannin, saponins and other antimicrobial agents. This study aimed to determine the effect of Agung Semeru banana peel extract as a natural hand sanitizer to inhibit fungal growth of Candida albicans. The antifungal activity test is carried out using the disk diffusion method. The use of this method is shown to measure the diameter of the area of ​​resistance that occurs around paper discs that already contain antifungal in accordance with the concentration in each treatment. The results showed that Agung Semeru banana peel is effective as a natural Hand sanitizer that was indicated by differences in treatment. Utilization of Agung Semeru Banana peel Extract  as a natural basic material hand sanitizer showed that no significant difference between treatments in inhibiting the growth of fungi Candida albicans, but a concentration of 7% (1,315 ± 0.0035c) showed better results compared to a concentration of 1% ( 1.2 ± 0.0a); concentration of 5% (1,208 ± 0,0023b) and 0% (1.2 ± 0,0a)

    The Effect of Hibiscus Leaf’s Adhesives on the Quality of Cow Dung Husk Charcoal Briquettes

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    The research to find out the good briquette adhesive has been done, however the results have not been satisfactory yet. This study aims to determine the effect of hibiscus leaf adhesive (Hibiscus rosa-sinensis L) on charcoal briquette organic charcoal. The materials used in the manufacture of cow dung husk charcoal briquettes are rice husk and cow dung. The research was conducted in biology garden of Science and Technology Faculty, Islamic State University of Sunan Gunung Djati Bandung from May to June 2015. The research was conducted by using Random Design Complete (RAL) Factorial 3 X 4 with twice repetition (duplo). There are 3 compositions of treatment rice husk: cow dung, that are: 1: 1, 3: 1 and 1: 3, and addition of 4 levels adhesive from hibiscus leaf (Hibiscus rosa-sinensis L.), that are; control (without adhesive), 10 grams, 15 grams and 20 grams. The results showed that the adhesive combination of hibiscus leaves and the composition of organic charcoal briquettes was highest at density of 0.89 g / cm3, calorific value of 2432.62 cal / g, carbon content of 25.49% and lowest at water content of 8.75 %, vapor content 32.89%, ash content 45%. The best treatment combinations were found in the ratio of rice husk: cow dung ie 1: 3 and adhesive concentration 15 grams with the best heat value of 2431,62 kal /g.The research to find out the good briquette adhesive has been done, however the results have not been satisfactory yet. This study aims to determine the effect of hibiscus leaf adhesive (Hibiscus rosa-sinensis L) on charcoal briquette organic charcoal. The materials used in the manufacture of cow dung husk charcoal briquettes are rice husk and cow dung. The research was conducted in biology garden of Science and Technology Faculty, Islamic State University of Sunan Gunung Djati Bandung from May to June 2015. The research was conducted by using Random Design Complete (RAL) Factorial 3 X 4 with twice repetition (duplo). There are 3 compositions of treatment rice husk: cow dung, that are: 1: 1, 3: 1 and 1: 3, and addition of 4 levels adhesive from hibiscus leaf (Hibiscus rosa-sinensis L.), that are; control (without adhesive), 10 grams, 15 grams and 20 grams. The results showed that the adhesive combination of hibiscus leaves and the composition of organic charcoal briquettes was highest at density of 0.89 g / cm3, calorific value of 2432.62 cal / g, carbon content of 25.49% and lowest at water content of 8.75 %, vapor content 32.89%, ash content 45%. The best treatment combinations were found in the ratio of rice husk: cow dung ie 1: 3 and adhesive concentration 15 grams with the best heat value of 2431,62 kal /g

    Correlation between Parity, Birth Interval, and the Frequency of Antenatal Care towards the Incidence of Low Birth Weight Babies in RSUD Dr. Mohammad Soewandhie Surabaya

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    Low Birth Weight (LBW) contributes to 60% to 80% of all neonatal and infant deaths in Indonesia whilst globally, prevalence of LBW is 15.5%, amounting to approximately 20 million LBW infants born each year. According to several previous studies, factors closely related with the incidence of LBW are high birth rates, shorter birth intervals, and low frequency of Antenatal Care (ANC) examinations. The objective of this study was to determine the correlation between parity, birth interval, and the frequency of ANC towards the incidence of LBW babies. This retrospective cross-sectional study using simple random sampling involved 95 mothers in RSUD Dr. Mohammad Soewandhie Surabaya between January and July 2016. Data was collected using medical records and analyzed using logistic regression test with p < 0.05. The result of this research showed there was no correlations between parity and incidence of LBW babies (p=0.162) nor between birth interval and incidence of LBW babies (p=0.574). There was a correlation between frequency of ANC and incidence of LBW babies (p=0.006). The physician should further improve early detection for LBW babies by monitoring high-risk pregnancy mothers routinely.Low Birth Weight (LBW) contributes to 60% to 80% of all neonatal and infant deaths in Indonesia whilst globally, prevalence of LBW is 15.5%, amounting to approximately 20 million LBW infants born each year. According to several previous studies, factors closely related with the incidence of LBW are high birth rates, shorter birth intervals, and low frequency of Antenatal Care (ANC) examinations. The objective of this study was to determine the correlation between parity, birth interval, and the frequency of ANC towards the incidence of LBW babies. This retrospective cross-sectional study using simple random sampling involved 95 mothers in RSUD Dr. Mohammad Soewandhie Surabaya between January and July 2016. Data was collected using medical records and analyzed using logistic regression test with p < 0.05. The result of this research showed there was no correlations between parity and incidence of LBW babies (p=0.162) nor between birth interval and incidence of LBW babies (p=0.574). There was a correlation between frequency of ANC and incidence of LBW babies (p=0.006). The physician should further improve early detection for LBW babies by monitoring high-risk pregnancy mothers routinely

    Diversity And Dominance Species Of Benthic Gastropod In Three Coastal Condition West Bali National Park

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    The aim of this research was to determine the composition, domination, and diversity of benthic gastropod in three coastal condition of West Bali National Park, Province Bali. This research conducted with transect method. The research datas were raised at three location with different type of substrate, there are Prapat Agung Beach, Karang Sewu Beach, and Cekik Beach. The substrate type was rough sand, cobble, and smooth sand. Every location has two transect which is upright the contur and then has a few plot too with the measure of every plot is 1 m2. The interval for every transect is 100 m, while for every plot is 10 m. Ponar dredge was use to raised benthic gastropod. Identification of benthic gastropod was refer to identification key. The result of this research showed that 25 species from 14 family of gastropod founded at three coastal condition. Euplica scripta was dominated in the first location with index of domination is 57,14%, Terebralia palustris was dominated in the second location with index of domination is 93,46%, and Hastula hectica was dominated in the third location with index of domination is 100%. According to the result, we can conclude that composition, domination, and diversity of benthic gastropod are interrelated with substrate type of the coastal.Penelitian ini diarahkan untuk mengetahui komposisi, dominansi, dan tingkat keanekaragaman gastropoda bentik di tiga kondisi pantai wilayah Taman Nasional Bali Barat, Provinsi Bali. Sampel gastropoda bentik diperoleh dengan metode transek. Data gastropoda bentik diambil pada tiga lokasi pantai dengan tipe substrat yang berbeda yaitu Pantai Prapat Agung, Pantai Karang Sewu, dan Pantai Cekik secara berturut – turut mewakili tipe substrat pasir kasar, batuan dan pasir halus. Pada setiap lokasi dibuat dua transek yang tegak lurus kontur dengan beberapa plot berukuran 1 m2. Jarak antar transek 100 meter dan antar plot 10 meter. Setiap plot dilakukan ekstraksi dengan Ponar Dredge untuk memperoleh gastropoda bentik. Identifikasi gastropoda bentik menggunakan buku kunci identifikasi. Hasil penelitian ini menunjukkan adanya 25 spesies dari 14 famili gastropoda yang ditemukan di tiga kondisi pantai. Euplica scripta mendominasi di lokasi satu sebanyak 57,14%, Terebralia palustris mendominasi di lokasi dua sebanyak 93,46%, dan Hastula hectica mendominasi pada lokasi tiga sebanyak 100%. Komposisi dominansi dan tingkat keanekaragaman gastropoda bentik sangat berhubungan dengan substrat di tiga kondisi pantai

    Antioxidant Activity of Nipah Endophytic Fungi (Nypha fruticans Wurmb) from Tanjung Jabung Timur Jambi

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    Nipah or Nypa fruticans Wurmb is a member of the Palmae tribe that lives in the Mangrove ecosystem and is known to have rich bioactive sources, one of which is antioxidants. The ability of N. fruticans as an antioxidant is also possible for endophytes associated with N. fruticans. This study aims to obtain endophytes that live in N. fruticans leaf tissue and find out their ability as antioxidant agents using synthetic free radicals 2.2 diphenil-1-pikrilhidrazil (DPPH). Samples taken at Nipah Panjang Tanjung Jabung Timur, Isolation and production were carried out at the Jambi University Faculty of Science and Technology Engineering and Biotechnology Laboratory, absorbance measurements using UV-VIS at λ 517 nm at the Jambi University CRC. The antioxidant activity of isolates was shown from the value of Inhibitor Concentration (IC50) which caused 50% loss of DPPH free radicals. From the results it was obtained that the six isolates (DN01, DN02, DN02, DN03, DN04, DN05, and DN06) value the antioxidant activity of endophytic extracts lower than vitamin C activity and still classified as weak. Even though endophytic Nypa fruticans are very weak, N. fruticans endophytic in all treatment concentrations still have the ability as antioxidants and optimization methods are needed to get good antioxidant values.    Keywords: Mangrove, Nypa fruticans Wurmb, endophytes, antioxidants, DPPHNypa fruticans Wurmb is a member of the Palmae tribe that lives in the Mangrove ecosystem and is known to have rich bioactive sources, such as antioxidants. The ability of N. fruticans as an antioxidant may also be owned by endophytes associated with N. fruticans. This study aims to obtain endophytes that live in the tissue of the leaves of N. fruticans and find out its ability as an antioxidant agent using synthetic free radicals 2,2 diphenil-1-picrylhydrazyl (DPPH). Samples were taken at Nipah Panjang Tanjung Jabung Timur, isolation and production were carried out at the Engineering and Biotechnology Laboratory of the Faculty of Science and Technology the University of Jambi, absorbance measurements using UV-VIS at λ 517 nm were carried out at CCRC Jambi University. The antioxidant activity of isolates is shown from the inhibitory concentration (IC50) which causes the loss of 50% of DPPH free radicals. From the results obtained that the six isolates (DN01, DN02, DN02, DN03, DN04, DN05, and DN06) have antioxidant activity values ​​of endophytic extracts that are lower than vitamin C activity and are still relatively weak. Even though the antioxidant activity of N. fruticans is classified as very weak, but at all concentrations the treatment of the isolate has the ability as an antioxidant and method optimization is needed in order to obtain a good antioxidant valu

    The Effectiveness of Papaya Leaf Extract (Carica papayaL.) Against Aphidsspin Tomato (Solanum lycopersicum) Plantation in the Natural Fence Area of South Sumatra

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    AbstractPagaralam is one of the tomato suppliers for the South Sumatera region. Tomatoes are often attacked by pests such as aphids. Aphids fleas (Aphid sp.) are one of the main pests besides that aphids are important vectors that can transmit viral disease. The use of chemical insecticides can damage agricultural land for the use of vegetable insecticide alternatives. Alkoloid, Flavoid, Saponin, and Tanin contained in papaya leaves (Carica papaya) can be used as a plant aphid insecticide. The sample in this study was a 2 month old tomato plant with 20 aphids in each tree. Treatment was given to 5 treatment with 5 replications. This treatment by giving different concentrations of papaya leaf solution in the crop of 0%, 15%, 30%, 45%, and 60%. To determine the effectiveness of papaya leaf solution (Carica papaya) as a vegetable insecticide aphid done spraying as much as 100 ml once spray with contact time observation 12 hours and 48 hours. The results showed that the application of papaya leaf juice was effective in killing aphids, so that high concentrations that gave greater precentage of aphids deaths ranged from 24 hours showed F count > and F table and received at level 1% means that papaya leaf juice was very influential on aphids death. Then the results of cofactor coefficient (KK) 24 hours and 48 hours were 10% and 4%, before using The Smallest Significant Difference Test (BNT) to see the effect of each concentration. BNT Test pre view, each concentration was very significant which had a different effect on the death of ticks to analyze probit LC50 to determine the effective concentration of killing 50% of the samples at 39,81%. This is related to every usage given such as concentration and monitoring time. Keywords: Papaya Leaf (Carica papaya); Papaya Leaf Solution Concentration; Fleas (Aphid sp.

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