Jurnal Biota
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Potential of Bacteriophages as Non-Alcoholic Antiseptic Hand Sanitizer
Full text linkBacteriophages, or phages, are viruses that can infect and replicate within bacterial cells, such as Escherichia coli. Phages demonstrate a strong ability to lyse host bacteria and exhibit high survivability, making them a promising innovation for use in non-alcoholic antiseptic products, such as hand sanitizer sprays and bacteriophage gels. This study aims to evaluate the effectiveness of bacteriophage-based hand sanitizer sprays and gels in reducing E. coli growth and total microbial colonies on palms, compared to commercial alcohol-based hand sanitizers. The method used in this study is a descriptive quantitative approach using an experimental method, specifically the Hand Sanitizer Spray and Bacteriophage Gel Test as Non-Alcohol Antiseptics. The average total bacterial colonies on male palms for the control treatment, phage gel sanitizer and commercial gel hand sanitizer were 1.95 x 104 CFU/mL; 1.15 x 103 CFU/mL; 2.55 x 103 CFU/mL, respectively, while on female palms, the values were 2.35 x 104 CFU/mL; 3.05 x 103 CFU/mL; 1.65 x 103 CFU/mL. The average total bacterial colonies on male palms for control treatment, phage sanitizer spray and commercial sanitizer spray were 1.30 x 105 CFU/mL; 2.05 x 103 CFU/mL; 9, 04 x 104 CFU/mL, respectively, while on female palms, the value was 1.58 x 105 CFU/mL; 8.36 x 103 CFU/mL; 8.79 x 104 CFU/mL. The results demonstrated that both bacteriophage hand sanitizer gel and spray significantly reduce bacterial colonies on palms, with phage-based hand sanitizer showing greater efficacy than commercial alcohol-based hand sanitizer
Phytochemical screening, in vitro and in silico antibacterial investigation of Elaeocarpus ganitrus extract
Full text linkThis study evaluated phytochemical composition, and in vitro and in silico antibacterial activity of Elaeocarpus ganitrus extract. Elaeocarpus ganitrus leaves, seed and fruit powder were extracted with absolute ethanol. Then, the extract was identified phytochemical compounds qualitatively and evaluated the antibacterial activity through in vitro against Staphylococcus aureus and E. coli. Molecular docking was conducted to evaluate the antibacterial mechanism of Elaeocarpus ganitrus extract. Elaeocarpus ganitrus leaves, seeds, and fruits extract presented positive tannin, saponin, cardiac glycoside, quinone, steroids, terpenoids, and anthocyanins. In vitro analysis performed Elaeocarpus ganitrus leaves strong inhibited Staphylococcus aureus growth and medium inhibition against E. coli. structure activity relationship revealed 14 of 72 compounds have high antibacterial activities. molecular docking of 7 compounds showed inhibition activity of D-alanin ligase of Staphylococcus aureus. Those compounds blocked the activity of D-alanine ligase at inhibitor sites of enzyme, and might be disrupted the cell wall synthesis. In conclusion, Elaeocarpus ganitrus contained several phytochemical compounds and has antibacterial activity both in vitro and in silico investigation
Identification of Tree Species and Their Potential as Carbon Stock in Three Urban Forests of Malang City, Indonesia
Full text linkThis study aims to identify tree species and their potential as carbon stock in three urban forests of Malang City. This research was conducted in three urban forests including the Urban Forest of Malabar, Velodrome, and Hamid Rusdi. Data were analyzed with an important value index (IVI) and Carbon stock estimation. There were 41 species of trees in three Malang City Forests. There are differences in tree dominance in the three Malang city forests, namely: the Malabar city forest is Albizia chinensis, the Velodrome city forest is Gmelina arborea, and the Hamid Rusdi city forest is Polyalthia longifolia. The highest value of carbon stock in the Malabar urban forest is A. Chinensis, 6,214.38 kg; in the Velodrome urban forest is Enterolobium cyclocarpum, 7,225.88 kg; and in the Hamid Rusdi Urban Forest is Samanea saman with a carbon stock of 4,757.01 kg
First Record of Milk thistle (Silybum marianum, Asteraceae) as Invasive Weed Species in Urban Green Areas of Addis Ababa City, Ethiopia
Full text linkWith increasing globalization and trade, the rate of invasive species entering and spreading has significantly increased globally. These include various invasive insect pests, pathogens, and weeds. Since three years ago, Milk thistle, Silybum marianum, an invasive weed species, has been observed widely in urban green spaces and roadsides in Addis Ababa city, Ethiopia. Weed surveys were conducted across 42 urban green sites located in Yeka, Lemi Kura, Bole, Nifas Silk-Lafto, Kolfie Keraniyo, Akaky Kality, and Gullele sub-cities during the main rainy season from June to September 2023, with the main objective of determining the current distribution of the weed in Addis Ababa city. The urban green areas were selected by using purposive sampling techniques. Field observations confirmed that Silybum marianum can invade and colonize wide habitats and is a highly competitive invasive weed to other important native plant species. High weed density rates and dense stands of weeds were recorded in the 34 urban green areas sampled. The survey result strongly suggests fast integrated weed management options such as mowing/tilling, weeding before the plant goes to seed, cutting (stems and/or seed heads), and burning (collected plant parts and/or seed heads) into a prepared burrow were done to minimize the spreading of the weed and its ecological impacts. The purpose of this survey study is to provide information about Silybum marianum to minimize or avoid its distribution and associated impacts by following sustainable invasive weed management programs in the city before spreading to other cities in the country
Interleukin-33 as a Potential Biomarker for Rheumatoid Arthritis: A Cross-Sectional Study
Full text linkRheumatoid arthritis (RA) is a chronic inflammatory autoimmune disease that affects about 1% of the general population. It is characterized by chronic, progressive, and systemic inflammation. Interleukin-33 (IL-33), is a pro-inflammatory cytokine that is believed to be involved in joint inflammation in RA. This cross-sectional research aims to determine if interleukin-33 (IL-33) could serve as a potential biomarker for RA diagnosis and evaluation of activity of RA. The research involved 132 patients with inflammatory arthritis, and their serum levels of IL-33 and anti-citrullinated peptide antibody (ACPA) were measured using ELISA. Other routine biomarkers, including C-reactive protein (CRP), erythrocyte sedimentation rate (ESR), and rheumatoid factor (RF), were also measured. The median (IQR) of IL-33 was significantly lower in patients with RA [10.576 pg/mL (7.920)] than in those with other types of inflammatory arthritis [12.896 pg/mL (5.700)]. The study also revealed a non-significant difference in IL-33 levels among the four disease activity groups according to DAS-28 ESR and DAS-28 CRP (P = 0.830, P = 0.340, respectively). Additionally, IL-33 had a significant negative correlation with age (P = 0.019) and diabetes mellitus (P = 0.032). To the best of our knowledge, this study is the first to evaluate IL-33 as a diagnostic tool, showing a sensitivity of 59.8% and a specificity of 72% at a cut-off value of ≤11.8207 pg/mL. IL-33 test alone is not sufficient for the diagnosis of RA or differentiating it from other types of inflammatory arthritis. Also, it cannot be used as a routine biomarker for the evaluation of RA activity
Natural Antioxidants Effect on the Performance of Erythrocytes, Leucocytes, and Hb Levels of Pregnant Mice Exposed to the Plastic
Full text linkPlastic is a widely used material with several toxic components, such as free radicals. HDPE plastic is considered safer but requires more attention due to increased usage. Exogen antioxidant is needed to neutralize free radicals. This research aimed to evaluate the effect of natural antioxidants on the performance of erythrocytes, leucocytes, and hemoglobin levels of pregnant mice exposed to plastic compounds. The study used a completely randomized design with six groups and four replicates each. Those groups were aquadest control, plastic chemical compounds (PCC) control, PCC + carrot juice, PCC + orange juice, PCC + purple sweet potato juice, and PCC + mung bean sprouts juice. The PCC was obtained from boiling HDPE plastic, while natural antioxidant juice was from pure sources. Pregnant mice were treated with 0.25 ml of each orally for 21 days, starting from the first gestational day. Blood was collected from a caudal vein. Erythrocytes and leucocytes were counted using a hemocytometer, and Hb levels were measured by the Sahli method. Blood cell morphology was assessed using Giemsa-stained blood smears. Statistical data were analyzed by one-way ANOVA using GraphPad Prism ver.10.0 software, while morphology data were analyzed descriptively. The results showed that PCC and natural antioxidants did not impact the number of erythrocytes, leucocytes, cell morphology, and Hb levels of pregnant mice. However, natural antioxidants may stabilize the erythrocyte total and Hb levels after the treatments are completed
Unraveling The Impact of Feed Protein Content on Catfish (Clarias sp.) Growth, Survival, Meat Quality and Gastrointestinal Histology
Full text linkCatfish is a highly favored source of protein in Indonesia. Catfish farming can be carried out using various types of feed and methods. This research aims to determine the effect of two types of fish feed with high (24%) and low (4%) protein content on several aspects of catfish cultivated using the pond/bucket culture method. The methods include catfish maintenance, physicochemical measurement, morphometric measurements, survival rate measurements, proximate testing, and histological preparations of catfish intestines and stomach. The results show that the TDS and pH values in the high-protein feed are higher than in the low-protein feed, while the temperature fluctuates. At the end of the observation, there is a significant difference in the morphometry of catfish fed with high-protein feed, where the body weight, head width, and body length are higher. The difference in body weight of catfish fed with high-protein feed is significantly higher (119.58±16.72 g) compared to those fed with low-protein feed (52.20±4.80 g). The average number of surviving catfish fed with high-protein feed is lower (27.60±8.23 fish) compared to the other group (44.00±2.55 fish). Proximate testing indicates that catfish meat with high-protein feed also has higher protein, fat, and carbohydrate content. Histological analysis shows that catfish with high-protein feed have longer villi and higher number of goblet cells, while the length of the gastric pits is lower. This research demonstrates that high-protein feed has an impact on fish quality, while ensuring water quality to reduce catfish mortality rates in catfish pond culture
Comparison of Two PCR Primer Sets for In-House Validation of GHSR Gene Variation Detection Employing Artificial Recombinant Plasmid Approach
Full text linkStunting is a significant global public health problem caused by long-term dietary deficits that affect many children worldwide. Both environmental and genetic factors, including variants in the GHSR gene, play a crucial role in stunted growth. This study used an artificial recombinant plasmid DNA method to evaluate two primer set combinations for identifying DNA variants in the GHSR gene. Selecting suitable primer sets for identifying GHSR genetic variants linked to stunting is essential, as evidenced by PCR and sequencing techniques. The target gene, based on the GHSR reference sequence, consists of eight DNA variations (ΔQ36, G57G, P108L, L118L, R159R, C173R, D246A, and A277P). A recombinant plasmid was created by inserting a 1000 bp fragment of the GHSR gene into the pUC57 backbone. Primer sets were chosen based on their capacity to amplify these eight genetic variations and were optimized and validated using PCR methods. PCR and bi-directional sequencing verified the existence of surrounding DNA and specific single nucleotide variants (SNVs). In our study, we discovered four changes in the DNA sequence (R159R G>A, C173R T>C, D246A A>C, and A277P G>C) using the E1_F2/E1_R3 primer pair. Additionally, a new combination of primers (E1_F1/E1_R3) effectively detected seven DNA sequence mutations (ΔQ36 del CAG, G57G C>T, P108L C>T, L118L C>T, R159R G>A, C173R T>C, and D246A A>C). We have developed a new combination of forward and reverse primers to identify seven SNVs in the GHSR gene, which could serve as a diagnostic tool in clinical laboratory settings
Flavonoids and Antioxidant Activities of Silver Nanoparticles of Extract Galaxaura rugosa
Full text linkAlgae Galaxaura rugosa contains phenolic compounds, flavonoids, β-carotene, and galactane sulfate, which are natural antioxidants. The development of antioxidants of red algae into silver nanoparticles (AgNPs) can improve their effectiveness in preventing ROS. The objective of this study is to synthesize silver nanoparticles using G. rugosa algae as well as to test the total flavonoid levels and antioxidant activity of G. rugosa silver nanoparticles. The method of research for synthesis silvernanoparticles using extract G. rugosa as a bioreductor. Characterization of silver nanoparticle G. rugosa using Particle Size Analysis (PSA) and UV-Vis Spectrophotometer. The total flavonoid and antioxidant activities of silver nanoparticles using UV- VIS spectrophotometer. The test of antioxidant activity using the DPPH method. The research results showed that silver nanoparticles using G. rugosa resulted in a color change of the soluble from green to yellow. The UV- VIS spectrophotometer measurement in the 570-580 nm wave range with a maximum wavelength of 406 nm. The measurement of the size distribution of the nanoparticles formed using PSA is an average of 11 nm. Total flavonoid silver nanoparticles of G. rugosa value of 36.21±0.65 mgQE/g higher than extract value of 32,12±0,79 mgQE/g. The antioxidant activity of IC50 of silver nanoparticle G. rugosa value of 26.658 ±1.44 ppm is very strong and the extract value of 46,128 ± 1.6 is strong category
Study of the Efficacy of Different Concentrations of Alcohol-Based Hand Sanitizer in Preventing Pathogen Transmission Through Palms
No full textAn integral part of the human body is the hands, which most frequently come into contact with various items; they can be an intermediary for spreading infections from hands to food and potentially eaten by humans. Maintaining hand hygiene is the right strategy to avoid this spread, which includes using an alcohol-based hand sanitizer. This study examines the potential for varying concentrations of alcohol-based hand sanitizer to transmit pathogens through the palms. The form of research is a laboratory experiment with an appropriate research design. Test the antibacterial potential of variations in alcohol-based hand sanitizer concentrations of 40, 50, 60, 70, and 70% using well diffusion and dilution methods. The tests were done in quadruplicates. The test bacteria used are Escherichia coli, Enterotoxigenic Escherichia coli, Salmonella typhimurium, Pseudomonas aeruginosa, Klebsiella pneumoniae, and Staphylococcus aureus. The results of the research showed that of the four variations in the concentration of alcohol-based hand sanitizer, the bacteriostatic ability against Salmonella typhimurium, Pseudomonas aeruginosa, and Klebsiella pneumoniae bacteria was at a concentration of 40% to 80%. The bactericidal activity at a concentration of 80% was demonstrated by Escherichia coli, Enterotoxigenic Escherichia coli, and Staphylococcus aureus. Bactericidal activity was demonstrated against Escherichia coli, Enterotoxigenic Escherichia coli, and Staphylococcus aureus at an 80% concentration