Jurnal Ilmu Ternak dan Veteriner
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Viability of Peranakan Etawah Liquid Semen Preserved in Tris Substituted with Various Energy Sources
Full text linkThe use of liquid semen for artificial insemination program of Etawah crossbreed goat (PE) is an alternative to replace frozen semen which is constrained by limited and expensive facilities. Production of liquid semen is faster than frozen semen, but the viability of liquid semen which preserved with a standard extender such as tris egg yolk is very short. The purpose of this study was to determine the viability of PE goat semen in egg yolk tris substituted with energy sources such as glucose, galactose, and mannose and to determine the most efficient energy source for semen preservation. This research was conducted from August to September 2018 at the Artificial Insemination Center in Lembang, West Java. This study was designed in a randomized block design (RBD) consist of three experimental groups divided into five groups. Fresh semen of PE goats were preserved using extender which energy source has been modified. Results showed that using glucose in PE goat semen extender produced the best motility among other groups (64.29 ± 9.2%). The highest viability was found in extender with fructose substitution (86.76 ± 2.3%). The longest viability of liquid semen was found in the extender with glucose substitution. It lasted for six days
Use of Infrared Thermography for Identifying Physiological and Hematological Conditions of Young Sapera Dairy Goats
Full text linkInfrared thermography (IRT) is an alternative solution that can be applied to replace invasive methods currently used in the monitoring of goats' physiological and hematological parameters. This study was done to compare and correlate the physiological and hematological conditions of young Sapera dairy goats and their correlations with results obtained by IRT. Four young Sapera dairy goats (weight of 26-28 kg) were kept in the individual rearing cage. Skin surface temperature (TS), rectal temperature (TR), body temperature (TB), heartbeat (HR), respiration rate (RR), and IRT at eyes, mouth, nose, legs, left body, right body, vagina, and vulva were monitored from 6 a.m. to 6 p.m. in 2 h intervals. Blood samplings were done at the beginning and the end of the obsevation time. Results showed that IRTs at several body parts were positively correlated with physiological parameters, except for heartbeat. Negatively correlation was observed in hematological parameters. The highest correlation (r = +0.85) was observed in the correlation between the results of the left rear leg IRT on TB. It was concluded that IRT can be applied to examine goats’ physiological conditions especially body temperature
The Effect of Morinda citrifolia and Arthrospira plattensis Powder on the Performance and Quality of Broiler Duck Carcasses
Full text linkThis study was aimed to investigate performance and quality of broiler duck carcasses fed with Morinda citrifolia and Arthrospira plattensis. A total of 168 two-week-old broiler duck with an initial average body weight of 463 ± 29.38 g and a diversity of 6.35 % were randomly allotted to 7 experimental groups with 4 replications each with 6 bird per replication. Treatments were T0 (basal diet as a control), T1 (basal diet + 0.2% of Morinda citrifolia powder (MP)), T2 (basal diet + 0.5% of Arthrospira plattensis powde(AP)) , T3 (basal diet + 0.2% of MP+ 0.5% of AP), T4 (basal diet + 0.4% of MP + 0.5% of AP), T5 (basal diet + 0.2% of MP + 0.1% of AP), T6 (basal diet 0.4% of MP + 0.1% of AP). Variables measured were feed intake, body weight gain, feed conversion ratio, carcass percentage, abdominal fat, and visceral organ. Data were analyzed for variance based on a Completely Randomize Design and continued with Duncan’s multiple Range Test for differences. Result showed that the treatments did not affect (P0.05) on feed intake, body weight gain and feed conversion ratio. The treatments also did not affect (P0.05) carcass percentage, abdominal fat, and visceral organ. The diet did not significantly improve performance and quality of broiler duck carcasses
Effect of Combination of Follicle Size, FSH and Cysteamine on In Vitro Production of Sheep Embryos
Full text linkThe participatory relationship among the follicle size, follicle stimulating hormone (FSH), and cysteamine (antioxidant agent) contribute to the production of embryos characterized by abundance and good quality. The aim of this study was to evaluate the efficacy of FSH, cysteamine and follicle size on in vitro embryo production of Awassi sheep oocytes. Follicles sizes were determined into two groups: small follicles (1-2 mm) and large follicles ( 2 mm). Oocytes were matured across two increasingly shared levels of FSH and cysteamine: A (40 ng/ml + 50 μM) and B (60 ng/ml + 100 μM). Results of the bilateral interaction showed significant differences across the follicle size (large follicles group) and the maturation treatment (B medium) in the rates of fertilization (highest value: 67.51%; p= 0.02), cleavage (highest value: 65.41%; p= 0.01), 2-16 cell stage (lowest value: 2.29%; p= 0.0001), blastocyst stage (highest value: 44.82%; p= 0.04), down to morula stage arrest (lowest value: 55.17%; p= 0.04) and Type I embryos (highest value: 52.87%; p= 0.03). Likewise, matured oocytes of small follicles group (B medium) attained the highest rate of morula stage (56.60%; p= 0.03). No significant differences were observed in Type II and Type III embryos. In order to obtain high yields of good quality embryos, it is advised to add FSH and cysteamine with levels of 60 ng/ml and 100 μM respectively to maturation medium of ovine oocytes obtained from follicles with a diameter 2 mm
Effects of Probiotic, Prebiotic, and Synbiotic Mixed Culture Based on Wheat Pollard on Productivity of Kampung’s Chicken
Full text linkThis research was aimed to assess the effectiveness of processed wheat pollard -based rations to increase the productivity of Kampung chickens raised until 8 weeks old. The research was carried out in a completely randomized design with 5 treatments and 4 replications. The treatments consisted of wheat pollard based ration (WP), wheat pollard based ration plus probiotic mixed culture (WPPro), wheat Pollard as prebiotic mixed (WPPre) , wheat pollard as synbiotic mixed culture ration 40% (WPS40), wheat pollard as synbiotic mixed culture ration 60% (WPS60) . The parameters observed were feed consumption, final body weight, feed conversion ratio (FCR), weight gain, nitrogen retention, income over feed and chick cost (IOFCC) and profiles of intestinal villus. Results showed a significant effect of the treatments on the final body weight, weight gain, nitrogen retention, IOFCC and profiles of small intestinal villus (duodenum, jejenum, ileum). It was concluded that the inclusion of 40% wheat pollard synbiotic mixed culture (WPS 40) in the ration was able to increase the productivity of kampung chickens reared until 8 weeks old
Direct and Maternal Genetic Trends for Some Productive and Reproductive Traits in Egyptian Buffaloes
Full text linkThis study was done to determine the direct and maternal genetic and phenotypic trends for productive traits such as first lactation milk yield (FLMY, kg), first lactation period (FLP, d) and first lactation daily milk (FLDM, kg), and reproductive traits such as age at first calving (AFC, mo), First days open (FDO, d) and first calving interval (FCI, d). Data were collected over consecutive 25 years (1991 to 2015) of 1104 first lactation of 135 sires and 482 dams maintained at Mahallet Mousa farms of Animal Production Research Institute. Data were analyzed by Animal model to determine genetic parameters for studied traits. Means of FLMY, FLP, FLDM, AFC, FDO and FCI were 1546.5kg, 189d, 7.9kg, 37.9mo, 120.8d and 428d, respectively. The direct heritability (h2a) for same traits were 0.25, 0.18, 0.24, 0.45, 0.18 and 0.19, respectively. Corresponding maternal heritability (h2m) for mentioned traits was 0.12, 0.19, 0.22, 0.25, 0.12 and 0.12, respectively. Genetic correlations (rg) among studied traits were varied between -0.19 to 0.38. Accuracy of predicted breeding value varied between 69 to 94, 0.37 to 94 and 42 to 91% for FLMY, FLP, FLDM, AFC, FDO and FCI of sires, cows and dams, respectively that revealed the genetic improvement could be actualized through each of cows or sires or dams. Additive and maternal genetic, permanent environmental and phenotypic trends were not significant for all studied traits. It indicated that it is important to set up a plan to improve genetic and environmental conditions thus, increasing productivity and realization of high profitability
Biological Evaluation of Some Plant Bioactives as Feed Additives to Replace Antibiotic Growth Promoters in Broiler Feeds
Full text linkAntibiotics (AGP) have been used as feed additives to promote growth and feed efficiency in poultry production. However, many countries include Indonesia now ban the use of AGP and attempts are made to replace the antibiotic to maintain good performances of broilers. Plant bioactives is one of the alternatives that could replace the AGP. An experiment was conducted in an attempt to replace the AGP in broiler feed with a mixture of some plant bioactives (liquid smoke of cashew nutshell, Phyllanthus niruri, and clove leaves). Eight (8) dietary treatments were formulated to have similar nutrients consist of negative control (NC), positive control (NC+AGP), diets supplemented with liquid plant bioactives in 3 levels and diets supplemented with powder plant bioactives in 3 levels. Each diet was fed to 6 replications of 10 birds each from 1 to 35 days old. The performances and the immune response of the broilers due to the treatments were observed. Results showed that the powder plant bioactives could not improve the performance of broilers. None of the feed additives (AGP or plant bioactives) affect the immune systems of the broilers. However, liquid plant bioactives in low dose improved the performance of broilers better than the AGP and therefore is suitable to replace the antibiotic as feed additives in broiler diet
Effect of Incubation Time During Sperm Sexing Process on Sperm Quality of Pasundan Bull
Full text linkThe research was conducted to evaluate the effect of incubation time on viability, plasma membrane integrity, abnormality, and DNA integrity of sexed Pasundan’s bulls sperm. The sperm sexing used 5% and 10% concentrations of Bovine Serum Albumin (BSA). A completely randomized design with three treatments and six replications was used in this study. The data were analyzed using variance analysis followed by Duncan’s multiple distance test. Parameter evaluated were sperm longevity, plasma membrane integrity (PMI), abnormality, and DNA integrity of sexed Pasundan bulls sperm. Results showed that incubation time gave significant effect (P0.05) on the longevity of sperm, but not on the PMI of Pasundan bulls sexed sperm. The incubation time of 45 minutes gave the highest value of longevity sperm on the upper layer (4.33 days) and the lower layer (4.17 days). Furthermore, the abnormality of sperm X in the upper layer was 4.00%-4.20% and the lower layer was 4.10%- 4.40%. Meanwhile, the DNA integrity of an upper layer was 98.16%-98.66%, and the lower layer was 97.83%-98.58%. It is concluded that 45 minutes of incubation time significantly affected the longevity of sperm, but not plasma membrane integrity, abnormality, and DNA integrity of Pasundan bulls sexed sperm
Effect of Mix Culture Bacteria and Fungi in Fermented Peanut Hulls-Based Feed Supplement on Physical Quality and In Vitro Rumen Fermentation Parameters
Full text linkThe purpose of this research was to determine the effect of the combination of mix culture bacteria (EM4®:E) and fungi (Trichoderma viridae:TV) on physical quality and in vitro rumen fermentation parameters of peanut hull-based feed supplements. Basal feed was divided into four treatments, which were: P0 (BF); P1 (E:25%+TV:75%); P2 (E:50%+TV:50%),; and P3 (E:75%+TV:25%); and each treatment had three replications. Feeds were fermented facultative anaerobically for nine days. The observed parameters were physical qualities (color, odor, fungi appearance and pH), and in vitro rumen fermentation parameters (rumen pH, microbial protein content, and total volatile fatty acid content). Data were analyzed using one way ANOVA design, and the significance of differences were tested using Kruskall-Wallis test for the physical qualities data and Duncan’s New Multiple Range Test (DMRT) test for in vitro rumen fermentation parameters. Results showed that the combination of E and TV at different level did not affect odor and the fungi appearance parameters (P≥0.05), however, it significantly affected P≤0.05) color change from blackish (1.64) in P0 to brownish in P1, P2 and P3 of 2.44; 2.69; and 2.80, respectively. The pH also decreased significantly (P≤0.05) by 10.67%. Treatment also did not affect the rumen pH, microbial protein content, and total volatile fatty acid content (P≥0.05). It is concluded that the combination of the EM4® 25%: Trichoderma viridae 75% on peanut-hull based feed supplement fermentation gives the best result on color and pH fermented feed product without affecting the rumen fermentation process
Efficiency of Dimethyl Sulphoxide and Ethylene Glycol on Subsequent Development of Vitrified Awassi Sheep Embryos
Full text linkThe use of cryoprotectants in vitrification would reduce the critical damages to the embryos, thus increase the survival rates. This research was conducted in the laboratory of reproductive biotechnology at the faculty of Agriculture of Aleppo University. The study aimed to evaluate the viability and survivability of early Syrian Awassi embryos under the influence of dimethyl sulphoxide (DMSO) and ethylene glycol (EG) following vitrification. Embryos were vitrified in three solutions of cryoprotectants (A: DMSO (3 ml), B: EG (3 ml), and C which was composed of a combination of DMSO (1.5 ml) and EG (1.5 ml)). After thawing, embryos that had been vitrified in C solution achieved the highest rates of cleavage (P 0.01) comparing with A and B solutions for 2-16 cell stage (50.00% Vs 30.77% and 36.36%), morula (9.00% Vs 44.44% and 40.00%) and blastocyst stage embryos (92.86% Vs 58.33% and 50.00%) respectively. Down to the hatching blastocyst stage, 2-16 cell stage vitrified embryos in C solution achieved an encouraging rate comparing with A and B solutions (39.20% Vs23.08% and 22.73% respectively). The rates of arrested embryos decreased significantly (P 0.05) after thawing across the three solutions especially the morula and blastocyst stage (0.00 and 3.70% respectively) (C solution). No significant differences were observed in the three types of embryos across all stages and solutions despite the large range among these rates. Given the apparent benefit of the participatory effect of cytoprotectants, it is advised to use a mixture of DMSO and EG (1:1) in vitrification of ovine embryos