Jurnal Ilmu Ternak dan Veteriner
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Campylobacter jejuni seroepidemiology in native chicken
Get PDFCampylobacter jejuni is responsible for about 90% of cases of Campylobacteriosis in humans with gastroenteritis. Healthy chickens can carry Campylobacter spp. in the intestinal tract. Efforts to reduce exposure to Campylobacteriosis by humans may be enhanced by knowledge of its prevalence in poultry. This study aimed to identify factors associated with seropositive response to C. jejuni in native chickens in Mataram. Detection of C. jejuni was accomplished using an immunochromatographic serological method. Association between Campylobacter jejuni seropositive response as the dependent variable with various independent variables was analyzed using χ² (Chi square) and Odds Ratio (OR). A total of 216 chicken samples were examined and 44 chicken owners were interviewed and their farms examined. Results showed the prevalence of serological response to C. jejuni in chicken samples to be as high as 35.6% and that as many as 70.5% of farms had affected chickens. Age of the chicken was the variable most closely associated with incidence of seropositive response, birds older than 3 months more likely to be affected. Variables at the farm level associated with variation in seropositive response were cage type, cage floor material, and origin of drinking water, surface water sources being less desirable. Key Words: Campylobacter jejuni, Seroepidemiology, Native Chicken, Matara
Effect of glutathione and bovine seminal plasma in lactose extender on viability of swamp buffalo frozen semen
Get PDFThe aim of the study was to investigate the effect on viability of frozen swamp buffalo semen of glutathione and bovine seminal plasma in lactose extender. Semen from two swamp buffalo bulls was collected twice weekly using an artificial vagina. Pooled, good-quality fresh semen was divided into three parts and centrifuged at 3,000 rpm for 15 minutes in preparation for three treatments-substitution of buffalo seminal plasma with zero, 50 or 100% bovine seminal plasma (BS0, BS50 and BS100, respectively). Each semen aliquot was then divided in two parts, on which was diluted with lactose extender containing 1 mM glutathione (GSH) and the other diluted with lactose extender without GSH (0 mM GSH). Extended semen from all six treatments was cooled to 5oC and then frozen in 0.25 ml straws. Mean motility percentages 0 and 30 minutes post thaw (PTM 0′ and 30′) with GSH were 38.33 and 34.29%, significantly higher (P 0.05) than treatments without GSH (31.67 and 25.95%). PTM 0′ and 30′ were also higher (P 0.05) with no substitution of bovine seminal plasma (BS0) than when buffalo seminal plasma was replaced with bovine seminal plasma at either 50 or 100%. Averages were 40.00 vs 34.46 and 30.54% (BS0 vs BS50 and BS100) at thawing and 36.96 vs 28.36 and 25.36% 30 minutes post-thaw. Mean percentages of live sperm (LD), intact plasma membrane (MPU) and intact acrosomal membrane (TAU) at thawing were not significantly different with or without addition of GSH. However,at 30′ post thawing, TAU and MPU were significantly higher in GSH treatments than inthose without GSH: 61.50 vs. 58.19% (MPU) and 59.81 vs. 57.38% (TAU). Mean percentages of LD, TAU and MPU 30′post thawing were higher with no substitution ofbuffalo seminal plasma (BS0) (P 0.05) than to BS50 and BS100 treatments. In conclusion, the addition of glutathione (GSH) improved the quality of frozen swamp buffalo semen, but the partial substitution of buffalo seminal plasma with bovine seminal plasmaprovided no beneficial effects. Key Words: Swamp buffalo, Semen, Antioxidant, Glutathione, Seminal plasm
Behaviour and production traits correlation of five breeds of sheep
Get PDFInformation about correlations among behavioral traits and production traits may facilitate using easily measured traits to be used as alternative criteria for indirect selection to improve other traits in sheep. The purpose of this study was to estimate correlations among behavioral and production traits of five breeds of sheep. A total of 128 head of weaned lambs and 168 ewes with nursing lambs of five breeds were used in this study, i.e. Barbados Black Belly Cross (BC), Composite Garut (KG), Local Garut (LG), Composite Sumatra (KS) and St. Croix Cross (SC). Temperament was assessed in a test arena, for subsequent association with growth rate and ewe productivity. Analysis of variance of production and behavioral traits among breeds was carried out using PROC GLM of SAS software Ver. 9.0. PROCCORR was used to estimate correlations among production and behavioral variables. Weaned lambs that were more docile towards the observer had higher daily gains. Ewes that bleated more frequently when separated from their lamb had higher lamb weaning weights and lamb survival than ewes bleating fewer times under the same conditions. In BC ewes, a positive correlation was observed between frequency of bleats and lamb survival. Key Words: Sheep, Behavior, Growth rate, Ewe productivit
Forage and seed production of Puero (Pueraria javanica) in a Different Light intensity level
Get PDFPuero (Pueraria javanica) is forage that can serve as a cover crop in plantations. The limiting factor for plant growth in the plantation is the light intensity, therefore the influence of light intensity on forage and seed production of Puero needs to be examined. Research was conducted at Kaum Pandak Research station of Indonesian Research Institute for Animal Production Bogor and Laboratory of Agrostology Faculty of Animal Husbandry, Bogor Agricultural University, for 16 months. Four levels of light intensity,i.e 100, 80,60 and 40% were applied, leguminosainous species Puero (Pueraria javanica), was used. The treatments were arangged in Randomized Complete Block Design with 3 replications. Data collected were analyzed by ANOVA and Duncan’s Multiple Range Test. Forage production was evaluated in one year. The forage quality and digestibility (invitro) were assessed. Seed production was recorded accumulatively from seasonal seed production during one year. Results show that light intensity affected (P 0.05) forage and seed production, chlorophyll a and total chlorophyll of Puero, but did not affect (P 0.05) quality and digestibility of Puero. The highest forage and seed production of Puero were obtained from full light intensity (100%). and seed production of Puero was affected (P 0.05) by light intensity. The seed quality of Puero was also affected by light intensity. The best seed quality of Puero was achieved by from 80% light intensity. Key Words: Light Intensity, Forage Production, Seed, Puer
Relationship of molting trait and egg production on crossbred Peking and Alabio ducks
Get PDFSelection program through 6 months egg production record as criterion of selection can improve number of egg in a year. However the selected population has still showed molting which is influenced the cessation of egg production, so that its production has not optimal yet. The research has conducted to further learn about the relationship between molting and egg production. This study used 90 females AP ducks (the crossbred of Alabio ♂ and Peking ♀) and 90 females PA ducks (the crossbred of Peking ♂ and Alabio ♀). Observed variables were onset of molting, the length of molting periode and the egg production for 48 weeks. The collected data were analyzed by analysis of variance, correlation and regression. The results showed that based on the starting time of molting the ducks can be categorized into two group i.e (a) molting duck group and (b) non-molting duck group. There were 63 PA ducks characterized non-molting and 21 molting, and there were 42 non-molting and 45 molting in AP ducks. The egg production of these two groups was significantly different (P 0.01). Since the molting was negatively correlated to the 48 weeks egg production (r = -0.896 for PA and -0.553 for AP), then this trait can be used as the selection criterion for egg production.The linear regression equation for PA of 48 weeks egg production = 80.7-0.369 molting duration, and for AP of 48 weeks egg production = 84 - 0.299 molting duration. Key Words: Molting, Egg Production, Duc
The effect of pineapple waste (Ananas comosus (L). Merr) subtitution on mixed basal diet of Elephant grass and calliandra on rumen ecosystem of sheep
Get PDFThe aim of this study was to evaluate the effect of pineapple waste substitution to mixed basal diet of Elephant grass and calliandra on rumen ecosystem. Pineapple waste was substituted to basal of Elephant grass and calliandra leaves (3:2) at the level of 0% (RA); 10% (RB); 20% (RC); 30% (RD); 40% (RE) and 50% (RF). In this experiment 24 Indonesian local male sheep (9-10 months old, 15.3 kg average body weight) were used, and were divided into 6 groups of dietary treatment (4 sheep each). Every group was offered one of the experimental diets (RA to RF) in a Completely Randomized Design. Pineapple waste was offered gradually for one month until the level of 50% (RF) was reached. The animals were adapted to experimental diets for about 14 days prior to the data collection period. Rumen fluids from each animal was taken (5 hours after morning feeding) for pH, ammonia concentration; bacteria and protozoa population analysis. The results showed that substitution of pineapple waste up to 30% had no effect on pH, but when the level was increased up to 40 and 50%, the pH (P0.01) decreased. Ammonia concentration was similar when 10% of the pineapple waste was included, then it decreased significantly when the waste was given up to 50% (P0.01). A decrease in bacteria population and an increase in protozoa population happened when the waste given was increased up to 50% but it wasn’t significant (P0.05). Increasing pineapple waste given increased population of amyllolytic bacteria but decreased the population of cellulolytic bacteria. On the Elephant grass and calliandra basal diet with the proportion of 3 : 2, the best substitution of pineapple waste was up to 20%. Key words: Pineapple Waste, Rumen, Bacteria, Protozoa, Ammoni
Physiological responses of Indigofera zollingeriana, a feed plant at different levels of drought stress and trimming interval
Get PDFThe objectives of this experiment were to evaluate the effect of drought stress and trimming intervals on physiological responses of Indigofera zollingeriana. The experiment was arranged in a completely randomized design (CRD), 3x3 factorial and each treatment had four replications. The first factor consisted of 3 level of drought stress i.e: 100% field capacity (FC) (as a control), 50% FC, and 25% FC. The second factor was comprised of 3 trimming intervals, those were at 60, 90 and 120 days. The observed variables were leaf water potential, relative water content, proline, and water soluble carbohydrate (WSC) concentrations. Data were analyzed by ANOVA and differences between treatments were tested by LSD. The results showed that there were no interaction (P0,05) between drought stress and trimming interval on all observed variables. Drought stress treatment significantly (P0,05) decreased leaf water potensial and relative water content, whereas proline, and water soluble carbohydrate (WSC) contents increased. Trimming interval significantly (P 0.05) on leaf water potensial, and water soluble carbohydrate, whereas the relative water content and proline content were not significantly. Key Words: Indigofera zollingeriana, Drought stress, Trimming interval, Physiologycal respons
Verotoxigenic Escherichia coli (VTEC) isolated from cow milk
Get PDFVerotoxigenic Escherichia coli (VTEC) strains are responsible for serious human illnesses. These strains are commonly found in milk. The aim of this study was to determine the occurrence of verotoxigenic E. coli in milk. A total of 351 milk samples, were collected from dairy farms in Bogor, Sukabumi and Cianjur. These samples were analyzed for VTEC using biochemical, serological and vero cell cytotoxicity assays. VTEC O157:H7 isolates were found in milk collected from dairy herds in Bogor and Sukabumi at rates 0.47% of 214 samples, 1.10% of 91 samples respectively, and none in Cianjur. Hemolytic E. coli isolates were found in 0.94% of 214 milk samples from Bogor, 2.2% of 91 milk samples from Sukabumi and none from Cianjur. From E. coli isolates, 53 isolates (67.95%) were verotoxigenic, consisted of: two E. coli O157:H7 isolates and 51 non O157:H7 isolates.Therefore this study showed the occurrence of VTEC in milk samples from dairy farms in Bogor, Sukabumi and Cianjur. Key words: Milk, E. coli (VTEC) O157:H7, Verotoxigeni
Seroprevalence of Q fever in sheep and goat in West Java area
Get PDFQ fever is a zoonotic disease caused by Coxiella burnetii, a species of bacteria that is distributed globally. Ruminant especially sheep and goats may play an important role in the transmission of the disease to human. The research of seroprevalence of Q fever in sheep and goats was carried out from August 2006 to March 2007 in West Java area. A total of 138 sera were collected; 69 sera from sheep and 69 sera from goats. The indirect immunoflourescent antibody test was used to determine the seroprevalence of Q fever. The seropositive based on the dilution of serum starting from 1 : 16. Seropositive were observed in 22 samples (31.88%) of sheep and 14 samples (20.28%) of goats. The highest titer of 1 : 128 was observed in 3 pregnant sheep. The results of the present study suggested that Q fever was endemic in West Java area. Key Words: Q Fever, Prevalence, Indirect Immunoflourescent Antibody Tes
The effect of combined probiotics with catalyst supplementation on digestion and rumen characteristic in Priangan sheep
Get PDFAn experiment was carried out to investigate the effect of combined supplementation of probiotics and catalyst on digestion and rumen characteristic in Priangan sheep. The trial was conducted using 16 heads of young male Priangan sheep with average initial weight of 18 kg in completely randomized design with factorial 2x2 and 4 replication. The first factor was two types of probiotics mixed with catalyst supplement, while the second factor was two levels of supplement percentage of catalyst at 0.5 and 1.0% of concentrate. The type of probiotics applied was probion and probiotics of buffaloes rumen microbes. The feeding level was set at 3% of body weight based on dry matter and consisting of forage (King grass) and concentrate at 50:50 ratio. The results indicated a significantly greater fibre digestion value (NDF) and proportion of acetate molar in the group of sheep fed combination of probiotics of buffaloes rumen microbes and catalyst supplement. It was concluded that the recommendation level of the combined rumen microbe probiotics with catalyst supplement in sheep ration was 0.5%. Key words: Probiotic-Catalyst Supplement, Digestibiliy, Rumen Characteristic, Shee