Jurnal Ilmu Ternak dan Veteriner
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Genetic diversity of native chicken based on analysis of D-Loop mtDNA marker
Get PDFProduction was carried out using control region/D-loop mtDNA marker. The base population of native chicken was selected from subpopulation at Cianjur, Jatiwangi, Depok, Bogor I, and Bogor 2. Samples from each population was 10 heads and 2 samples Green Jungle Fowl (Gallus various) from East Java as out Group samples. Two primers binding conserved tRNA Phenylalanine gene and tRNA Glutamine gene were DNA Heavy stranded HI255 (5'-CATCTTGGCATCTTCAGTGCC-3') and DNA Light stranded Ll6750 (5'-AGGACTACGGCTTGAAAAGC-3') was used to amplify D-Ioop mtDNA chicken. PCR-RFLP methods with 6 restriction enzymes 4 cutter such as, Alul (AG↓CT), Hpall (C↓CGG), Mbol (↓GATC), Rsal (GT↓AC), NlaIII (CATG↓) and HaeIII (GG↓CC) were used to detect polymorphism within and between subpopulation. Result of experiment show that mtDNA which was amplified by PCR was 1320 bp, consist of 1227 bp control region/D-loop, 45 bp tRNA Glutamine gene and 48 bp tRNA Phenylalananine gene. PCR product which were digested from 6 endonucleases enzyme show that native chicken within and between population was monomorphic and if its compare with Green Jungle Fowl was polymorphic. Key words: Native chicken, genetic diversity, mtDN
The development of fowl cholera vaccine: II. Pathogenicity and vaccine protection of Pasteurella multocida local isolates in experimental ducks
Get PDFPasteurellosis or fowl cholera in ducks occurs sporadically along the year in many high duck population areas of Java and other parts of Indonesia. Some isolates of Pasteurella multocida from ducks and chicken are kept at the BALITVET culture collection. The aims of this research were to evaluate the pathogenicity of local isolates and imported strains of P. multocida and to study the pasteurellosis local isolate vaccine and protection assay in ducks. Two imported strains of P. multocida (BCC 1359, BCC 1362) and 6 local isolates (BCC 299, BCC 2331, DY1, DY2, 12TG, 15TG) were used in this study. In the pathogenicity assays the imported strains and local isolates were activated in mice and in duct and then in brain hearth infusion broth containing 5% normal sheep serum. Each of broth culture was diluted, each dilution (102 and 104) of strains or isolates was injected intraperitoneally into a group of normal ducks. Antigen for vaccine, each was produced in sheep blood (5%) agar. Cells were harvested and killed with 0.1% formalin. Monovalent, bivalent, and polyvalent vaccines were prepared, at concentration equal to the Macfarland standard tube No 10, and each was adjuvanted with alhydrogel at final concentration of 1.5%. Each vaccine type was injected into a group of 10 week old ducks (8 animals per group), with 0.2 ml/injection. Four weeks later each animal in group were boostered with the same vaccine, dose, route as the previous injection. Before vaccination each animal was bleed through wing vena, then every two weeks, serum was collected and stored at -20oC. Two weeks after boostered, three days after the last blood sample collection, half animal of each group were challenged intraperitonelly with the BCC 2331 and half with DY2 live broth culture. The pathogenicity assays showed the only BCC 2331 and DY2 killed the experimental ducks, the other did not. The animals vaccinated with either BCC 2331, DY2 or bivalent (BCC 2331+DY2) vaccines were protected with either life bacterial challenged of either BCC 2331 or DY2 local isolates. It is likely, P. multocida BCC 2331 and DY2 isolates can be used for pasteurellosis candidate vaccine used in Indonesia, but it still needs more studies in the immunological of protective antigens. Key words: Pasteurella multocida, fowl cholera, ducks, vaccine, protectio
Carcass profile and chemical characteristic of male Kacang goat (Capra aegragus hircus)
Get PDFThe purpose of this research was to characterize the carcass profile and chemical characteristic of male Kacang goat (local name for Capra aegragus hircus) from two different slaughter ages, namely 1.5 and 1.5 years old. Kacang goats generally is slaughtered for consumption at these ages. The body part used for chemical analysis was the carcass leg. Data was analyzed statistically by t test. Results showed that there was no profile differences of the carcass from both group ages obtained from similar rearing system and at common range of body weight for slaughtering. Chemical characteristics measured by the contents of protein, fat, moisture, ash and cholesterol level in goat meat showed no differences between the two slaughter ages. Dominant amino acids found in the goat meat was glutamic acid, aspartic acid, leucyne and lysine. Saturated and unsaturated fatty acid profiles in the goat meat did not show any different between the two slaughter ages, except for eicosatrienoic acid, in which the meat from the younger goat had significantly higher content of eicosatrienoic acid. The meat of male Kacang goat of both groups is a potential source of healthy meat, based on its low fat content, functional amino acid composition, higher desirable fatty acids and fatty acid ratio of omega 6 : omega 3 which corresponds to the values recommended by American Heart Association 2008. Key Words: Male Kacang Goat (Capra aegragus hircus), Carcass, Cholesterol, Amino Acids, Fatty Acid
Serotype variation among infectious bronchitis viral isolates taken from several areas of Java
Get PDFInfectious bronchitis (IB) is an acute highly contagious viral respiratory disease of poultry caused by virus belongs to the family of Coronaviridae. The virus consist of many serotypes with low level of cross-protectivity among serotypes. Field data showed that the outbreaks of IB were frequently reported in chicken flocks, although vaccinations against the disease have been practiced. Hence, the study on serotype relationship among isolates of the viruses is essentially required. The aim of this study was to isolate and characterize IB viruses from chicken flocks in some areas of Java. Isolation of the virus was carried out in nine-day old embrionated chicken eggs and identified by means of agar gel precipitation (AGP) tests against standard antisera to IB virus. The serotypes of the IB viral isolates were determined by cross-neutralization tests in nine day old embryonated chicken eggs using r value derived from homologous and heterologous serum titres as criteria. This study obtained 12 IB viral isolates which were identified on the basis of the ability to cause lesions in chicken embryos and positive to agar gel presipitation test against standard positive antiserum to the virus. Based on the cross-neutralization tests in embryonated chicken eggs, isolate I.9 was formed to have relationship closed to Mass-41 serotype, while I.2, I. 3, and I.7 isolates were closely to the serotype of Con-46. Virus isolates (I.5, I.14, I.24, and I.25) were decided to have no serotype relationships to either Mass-41 or Con-46 serotype. Since the I.5, I.14, I.24 and I.25 isolates were not neutralized by antisera against the previous identified local infectious bronchitis viral isolates, and that were considered to be distinct serotype to the previously identified local IB viral isolates. Key words: Infectious bronchitis, virus, embryonated egg, cross neutralization test
The effect of feeding mixtures of banana trunk and soybean meal on lambs performance
Get PDFThis study was intended to determine the effects of increasing the ratio of banana trunk to soybean meal on intake, nitrogen excretion and lambs performance. Sixteen lambs (averaging 21.21 + 1.69 kg) were pen individually and fed a basal diet consisted of chopped fresh king grass and commercial concentrate and unlimited access of water. Lambs were randomly assigned to one out of four different ratio of banana trunk to soybean meal as feed supplements, i.e. (R0) 0:1; (R1) 1:1; (R2) 2:1 and (R3) 4:1. Results showed that the inclusion of banana trunk had no adverse effect on lambs performance. As it is expected, altering the ratio of banana trunk to soybean meal increased (P 0.05) dry matter intake of forage as well as total dry matter intake. The digestion coefficient of dietary ration was not affected (P 0.05) by feed supplement. Despite of no difference was observed in the coefficient of nitrogen digestibility, apparent fecal and urine N-output were significantly (P 0.05) affected. The highest ratio of fed supplement (R3) tended to decrease nitrogen retention. Overall data indicated that lambs offered basal diet supplemented with R2 treatment accumulated more N per-day, than those fed other treatments, and consequently lambs performance was the best on those fed R2 treatment. Key words: Banana trunk, protected protein, lamb
Productive performance of sheep under intensification program using a twice yearly lambing schedule
Get PDFA total of 350, three to four year old Daldale ewes were allocated into two treatment groups balanced for age and liveweight. Group TY was joined twice yearly (April-May and September-October) and group OY was joined once yearly (April-May). Three percent of fertile rams fitted with ewe marking crayons and harnesses were used in each group during the six weeks joining period. The productive performance were recorded during three years period. The average raddling rate, lambing rate and number of lambs born per ewe in the TY group were signicantly higher than those of the OY group (P0.05). However, birth weight, weaning weight and pre-weaning growth rate of the lambs born from the TY group were significantly lighter than those of the OY group (P0.05). There was no significant difference on wool production between the TY and OY groups (P0.05). Total weaning weight of lambs horn from both the TY and OY groups for the first, second and third year of research were 2.29 vs 2.49 tons, 2.50 vs 2.63 tons and 1.43 vs 2.34 tons, respectively. It was concluded that the Daldale ewes showed a high proportion of oestrual activity in all year round indicating their suitability for the use in the intensification of sheep husbandry. However, the twice yearly lambing program is still need further research particlularly in selecting breed and other supporting factors
The detection of infectious bronchitis viral antigen by means of immunohistochemical technique in broiler chicken infected with I-269 IB isolate or injected with H-120 live vaccine
Get PDFA study was carried out to detect the antigen of infectious bronchitis vius (IBV) in broiler chicken by means of immunohistochemical technique. A total of 150 - fourteen days old broiler chicken were divided into three groups i.e. 50 chicken were infected with an IB isolate of I-269, 50 chicken were injected with H-120 life vaccine, and 50 chicken served as un-treated control. Clinical signs and gross pathological changes were observed. Each of five chicken of each group were necropsied at 1, 2, 3, 4, 7, 10, 14, 21, 28, and 35 day(s) post infection/vaccination. The antigen could be detected at one day through 35 days post vaccination/infection. In the vaccinated group, histopathological lesions and the detected antigen were minimal. In contrast, the infected chicken showed varied histolopathological lesion in accordance with the numerous antigens. The antigen were observed in the lymphocytes/macrophages in the trachea, lungs and kidney, and in the epithelium of trachea, alveoli, broncheolus and tubular sitoplasm of the kidney of both vaccinated and infected groups. In the infected group, antigen was also detected in the lymphocytes and macrophages of the affected organs. Key words: Infectious bronchitis, broiler chicken, I-269 IB isolate, immunohistochemistr
Improvement of nutritive value of acacia mangium bark by alkali treatment
Get PDFBark, especially from Acacia mangium is a by-product from wood processing industries that commonly found in Indonesiaand in big amount will cause environmental problems. One of the alternatives to utilize bark is for animal feed. The aims of this experiment are to improve the nutritive value of bark by alkali treatments (urea and sodium hydroxide) and to determine the level of substitution of elephant grass by bark. The experiment consisted of 3 in vitro studies and 1 in sacco study. In vitro studies consisted of 1) the use of urea or NaOH by wetting and incubation-method, 2) the use of different concentration of Na OH (0-4%) by soaking method, 3) determination of substitution level of elephant grass by treated bark. In sacco study was conducted at 0, 6, 12, 24, 48 and 72 hours of incubation to compare the degradation of treated bark to elephant grass. The results show that urea treatment did not improve DM or OM digestibilities of bark. Soaking bark in 4% NaOH solution was more effective than wetting and incubation-method in improving in vitro digestibility. (49.26% vs19.56% for soaking and dry-method, respectively). In sacco studyl shows that treated bark had a very high solubility at 0 hour incubation but the degradation at 72 hours incubation was not significantly different from that of 0 hour incubation. The gas produced at in vitro study of treated bark was very low indicated that there was no degradation of bark at all. The level of substitution of elephant grass by treated bark up to 30% gave a non-significant digestibility value to that of 100% elephant grass. In conclusion, bark after tannin-extraction was a better feedstuff for animal feed. The soaking method in 4% NaOH solution improved the digestibility of bark significantly and the level of substitution of elephant grass by treated bark was 30%. Key words: Bark, acacia mangium, urea, sodium hydroxide, digestibilit
Performances of composite genotype resulting from crossingbetween local sumatera and hair sheep under confinement condition
Get PDFUnder semi-intensive conditions, the composite genotype (K) (25% Barbados Blackbelly; BB, 25% St. Croix; SC, 50% Sumatra Thin Tail; DETS) showed relatively have similar weaning weight as compared to other contemporary crossbred, therefore under intensive conditions (confined), they are expected to have better performances. The objective of the study are to find performances of the second generation composite genotype lambs and the first generation performances of the ewes, as well as several genetics parameters under intensive conditions, and compared to the Barbados Blackbelly Cross (BC) as contemporary crossbred. The growth of pre- and post-weaning of BB crossbred (BC) and the second generation of composite genotype (K-F2) are relatively the same. Growth curve using Von Bertalanffy model with the equation of Wt (t) = A*(1-B*e**(- K*t)), show that the equation for BC is Wt (t) = 26.8(1-0.92e**(-0.01t) and for K-F2 is Wt(t) = 26.1(1-0.92e**(-0.01t). This equation indicate that the growth of BC and K-F2 is not significantly different. The BC and K-F2 genotype reach maturity at the weight of 26.8 and 26.1 kg, respectively, and both with rate of reaching maturity of 0.01. Mating weight and weight at lambing of those two genotypes are around 29 - 30 kg dan 31 kg, respectively. The mean of litter size of BC and K-F1 are 1.52 and 1.48, respectively or around 1.5. The average litter size at weaning are 1.39 and 1.34, respectively for BC and K-F1. This means that pre-weaning mortality are around 5.37 and 9.76%, respectively for BC and K-F1. The lambing interval of BC and K-F1 are 211 and 223 days, or 0.58 and 0.61 year, respectively. Therefore the reproductive rate (RR) of the respective genotype are 1.39/0.58 = 2.39 and 1.34/0.61 = 2.19, respectively. The average of litter weight at weaning (LWW) of BC x BC, SC crossbred (HC) x BC and K-F1 x K-F1 are 11.62, 12.60 and 11.83 kg, respectively. Therefore based on the ewe genotype the LWW of BC and K-F1 are 11.74 and 11.83 kg, respectively. Flock productivity (FP) for those two genotypes per year are 20.24 and 19.39 kg, respectively for BC and K-F1. Dam productivity index (DPI) that calculated as /ewe weight for those two genotypes are 0.67 and 0.66, respectively for BC and K-F1. Meanwhile, the flock efficiency index (FEI) that calculated as FEI = FP/ewe weight0.75 for the respective genotypes are 1.57 and 1.54 for BC and K-F1. The estimates of repeatability of litter weight at weaning show relatively low value (0,092), therefore the estimate of MPPA (Most Probable Producing Ability) for predicting the future productivity is relatively the same. The estimate of MPPA for litter weight at weaning (LWW) for the respective genotype are 11.79 and 11.81 kg, respectively for BC and K-F1. Results of the study show that performances of composite genotypes (F) and the contemporary crossbred (BC) are relatively similar in the traits of growth, dam productivity and the estimate of MPPA. Key words : Composite sheep, performance of production and reproduction, intensif conditio
Adjustment factors of birth weight and four postnatal weights for type of birth and rearing, sex of lambs, and damage
Get PDFMany factors contribute to variation in lamb weights . Factors such as age of lamb, sex, type of birth and rearing, and ewe age at lambing obscure genetic differences among lambs . Variation associated with these factors make selection for increased weight less effective . To improve selection response, selection must be based on genotypic rather than environmentally induced superiority . It is well-documented that corrections for classifiable sources of variation render selection and culling more accurate, thereby increasing rates of genetic improvement . The objective of this study were to develop within breed adjustment factors for post-natal lamb weights . Field records from Suffolk and Dorset lambs collected in the U.S . National Sheep Improvement Program (NSIP) from 1986 through 1989 were used to develop within-breed birth/rearing type, lamb sex, and dam age adjustment factors for birth weight (BW) and 30-d (W3o), 60-d (Woo), 90-d (W9o), and 120-4 (Wtm) weights. Data were analyzed using general linear models . Within breed and age group adjustment for lamb-age differences were suggested because growth rates vary among breeds and among the four postnatal lamb-age groups . Within-breed multiplicative adjustment of the five traits for the effects of birth/rearing type, lamb sex and dam age were suggested because variances of these factors were significantly (P0.01) heterogenou