Jurnal Ilmu Ternak dan Veteriner
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Microencapsulation of bovine spermatozoa: Cryopreservation of microencapsulation sperm using glycerol
Get PDFCryopreservation of spermatozoa has been used to preserve spermatozoa in very low temperatures. Glycerol is intracelullar cryoprotectant usually used in Tris citrate containing 20% egg yolk (TCEY), while TCEY-1.5% alginate was used as encapsulation media. The effect of alginate in microencapsulation process and glycerol concentration on viability spermatozoa (motility (%M), live sperm (%L) and intact apical ridge (%IAR) were studied in two steps. In the first step, the effect of alginate and microencapsulation process was studied. Three treatments of this step were: 1) TCEY add with semen (as control), 2) TCEY-Alginate add with semen, 3) TCEY-Alginate add with semen and microencapsulated, followed by conventional sperm cryopreservation using TCEY 7% glicerol. The second steps were done to evaluate the effect of two glycerol concentrations (5 and 7%) and two duration of equilibration time (3 and 4 hours). Result of these experiments showed, that microencapsulation was significantly alter the percentage of post-thawing motility (%M) and intact apical ridge (%IAR). The motility of microencapsulated sperm vs control was 44.7 and 50.83% respectively, whereas IAR 79.33% and 83.50% on microencapsulated sperm and control. There was evidence that alginate act as extracelullar cryoprotectant by protecting sperm during freezing. The mean of decreasing percentage of %M and %IAR in TCEY-Alginate were lower (15.97 and 6.44%) than control (23.80 and 7.37%). The effect of glycerol concentration and equilibration time on the viability of sperm was not significant different. There was no significant interaction of glicerol and equilibration time on the viability of spermatozoa. In conclution, microencapsulation processes altered the viability of sperm, alginate had an important roles as extracelullar cryoprotectant. Moreover the cryopreservation of microencapsulated sperm might be done using 5 or 7% glycerol in 3-4 hours duration of equilibration time. Key Words: Sperm, Alginate, Microencapsulation, Glycerol, Equilibration Tim
Production and quality of Murdannia bracteata biomass as impact of magnesium foliar fertilizer
Get PDFMurdannia bracteata is one of potential forages for ruminant that has not been studied yet. This forage contents high mineral and it can be as fungtional feed. The aim of this study was to evaluate the growth and productivity, chlorophyll and minerals content of Murdannia bracteata caused by magnesium foliar application. The experiment was arranged in randomized complete design with five treatments and 4 replications. The application of magnesium level was arranged into: 0 ppm, 2000 ppm, 4000 ppm, 8000 ppm, and 12000 ppm. The study was conducted in a greenhouse, field laboratory of Agrostology, Faculty of Animal Science, Bogor Agricultural University. The result showed that growth, productivity, and chlorophyll content were not significantly (P0.05) affected by increasing magnesium level. Increasing magnesium dossage resulted in decreasing calsium, potassium, and zink content (P0,05), but not on phosphor and sodium (P0,05). In conclusion, increasing magnesium dossage up to 12000 ppm did not significantly affect growth, productivity, and chlorophyll content. However, increasing magnesium level decreased calsium, potassium, and zink content
Production and purification of streptavidin with higher biotin-binding activity
Get PDFThe objective of this study was to develop practical, efficient method for production, purification and assay of binding activity of streptavidin. Streptomyces avidinii was first propagated on agar plates, the bacterial cells on the agar were scrapped and suspended in a defined synthetic media (4.4 ml/cm2). After 7 days agitation on a rotary shaker (200 rpm/min) at room temprature (≈28°C), the bacterial cells in the culture were pelleted. The culture supernatant was concentrated to 1/62 original volume with 75% saturation ammonium sulphate. After intensive dialysis against ammonium carbonate buffer pH 11, the suspension was loaded into an iminobiotin agarose column chromatography. The adsorbed protein (streptavidin) was eluted with sodium acetate buffer, pH 4, and the eluate was concentrated with an ultrafiltration divice and suspended in PBS. The strepatavidin-binding activity was assayed by a competitive ELISA, a competition between streptavidin in the sample and the HRP-streptavidin conjugate for the biotin (biotinyl IgG) immobilised on wells of a microtitre plate. The detection limit of this assay measured 0.16 µg/ml streptavidin. The method developed in this study produced 160 µg/ml streptavidin in the culture supernatant. After concentration with the ammonium sulphate, the streptavidin concentration increased to 4 mg/ml (69% recovery). At the final step of purification, streptavidin with 10 mg/ml concentration was obtained. The purity of the streptavidin was higher (95%) with a recovary of 19%. The purified streptavidin in this study appeared as a dimer core streotavidin on SDS PAGE and its binding activity was twice as high as that of a commercial one
The effect of feeding pre-starter on performance efficiency of local chicken (KUB chicken)
Get PDFAn experiment in feeding pre-starter diets was carried out on local chicken (KUB chicken) raised to the age of 84 days. Four hundred and eighty day-old KUB chicks were allocated to experimental diets of P1 = standard diet without pre-starter; P2 = OASIS® pre-starter for 48 hours feeding; P3 = COBA-1, a mixture of 76.3% yolk powder, 0.76% inulin powder, 7.63 % honey and 15.3% tomato sauce, for 24 hours feeding; P4 = P3 given for 48 hours feeding; P5 = fresh papaya for 24 hours feeding; P6 = P5 for 48 hours feeding; P7 = fasting for 24 hours and P8 = fasting for 48 hours. Following treatment, the chicks were then fed with standard diet, containing 17.5 % crude protein with 2800 kcal ME/kg up to the end of the experiment. Results showed that the group of chicken on pre-starter diet of ripe papaya fruit (P5 and P6), responded better EPEF (European Performance Efficiency Factor) value of 442 and 356 g/bird, respectively in chicken of P5 and P6. This better response was due to particularly higher viability and the efficiency in utilization of feed
Utilization of palm oilsludge in poultry diet: 3. Inclusion of fresh or dried fermented palm oil sludge in broiler’s diet
Get PDFDrying proces, especially with heating often affects the nutritive values of feed ingredients. Therefore, this experiment was designed to study the responses of broiler chickens when fed with ration containing fresh or dried fermented palm oil sludge(FPOS). Experimental diets with different levels of fresh or dried FPOS (5, 10, and 15% equally to dried FPOS) were formulated with similar nutrient contents. A control diet with no FPOS was also included. Each diet was fed to 30 broiler chickens (5 replicates of 6 birds) for 5 weeks. Performances (body weight, feed consumption, feed conversion, and mortalities) of chickens were recorded. Carcass percentage and abdominal fat content was also measured at the end of feeding trial. Data obtained were subjected to analysis of variance in a completely randomized design and different between means were tested by orthogonal contrast procedures. Results of the experiment showed that body weight gain (BWG) of control birds was not significantlydifferent with BWG of birds fed with FPOS. Birds fed with dried FLS gain more weight than those fed with fresh FPOS (1048 vs 981 g/bird). Increasing of dietary FPOS levels decreased BWG, but 10% inclusion was still tolerable. Feed consumption of control diet was significantly (P0.05) less than feed consumption of diet with FPOS. Increasing of dried FPOS to 15% did not affect feed consumption, but increasing of fresh FPOS significantly increased feed consumption. Feed conversion (FCR) of control diet was significantly better than the FPOS diet (2.07 vs 2.13). Increasing levels of dried FPOS from 5 to 10% did not affect the FCR, but further increasing to 15% significantly worsen the FCR. Increasing of fresh FPOS from 5 to 10 or 15 significantly worsen the FCR. Dry matter intake, mortalities, carcass percentage, liver weight, and abdominal fat levels of broilers were not significantly affected by dietary treatments (P0.05). Conversion of feed dry matter to body weight gain of control diet was not significantly (P0.05) different with those diets with FPOS. However, dry matter conversion of dried FPOS was significantly better than the fresh FPOS. Increasing levels of FLS from 5 to 15 significantly worsen the feed dry matter conversion but not with inclusion of 10% FPOS. Therefore, it is concluded that there is no superior advantage of feeding fresh FPOS as compared with dried FPOS. Inclusion of 10% dried or fresh FPOS in the diets did not affect growth performances of broiler chickens. Key words: Palm oil sludge, dried, fermented, broiler
Vaccination trials in sheep against Chrysomya bezziana larvae using the recombinant peritrophin antigens Cb15, Cb42 and Cb48
Get PDFRecombinant forms of a number of peritrophic membrane proteins from the screwworm fly Chrysomya bezziana have been assessed in vitro and in vivo for their efficacy as antigens in vaccination against the tissue-invasive, larval form of the parasite. The proteins included Cb15 and Cb42 expressed in Escherichia coli and Cb48 expressed in both Escherichia coli and Pichia pastoris. In all cases, the in vitro assays of larval growth on serum from vaccinated sheep failed to show inhibition of larval weight gain or any detrimental effect on larval survival relative to controls. Chrysomya bezziana Cb48 has a significant degree of sequence identity with the antigen PM48 from Lucilia cuprina. Feeding Lucilia cuprina larvae on antisera to Cb48 induced a small but statistically significant reduction in weight gain, as does feeding on antisera to PM48. In vivo, larvae feeding on sheep vaccinated with Escherichia coli-expressed Cb15 and Cb42 and Pichia pastoris-expressed Cb48 showed marginally greater weight gain and survival which was equal to or greater than that on non-vaccinated sheep. The significance of these observations is discussed. Key words: Chrysomya bezziana, recombinant antigen, peritrophin, vaccinatio
Effect of temperature and time of pasteurization on the milk quality during storage
Get PDFA study on the effect of temperature and time of pasteurization on the milk quality during storage was carried out using fresh milk. The aim of the experiment was to asses the storage time of pasteurized milk for consumption without nutrient losses. A completely randomized factorial design, 2 x 8 was used, with pasteurization temperature (T), consisted of 2 levels, the low temperature long time (LTLT), i.e. fresh milk was warmed at 65oC for 30 minutes (T1) and the high temperature short time (HTST), i.e. fresh milk was warmed at 71oC for 15 seconds (T2); and storage time (S), consisted of 8 levels, i.e. 0, 3, 6, 9, 12, 15, 18, and 21 hours respectively, as the factors, with 3 replicates. Parameters measured were alcohol test, water, fat, and protein concentrations, and microbial population of pasteurized milk during storage. Data were analyzed using analysis of variance and simple linear regression. The result showed that water and fat concentrations and microbial population was not significantly different (P0.05) in pasteurization temperature treatment, but was significantly different (P0.05) due to storage time treatment. Meanwhile, the protein concentration was significantly different (P0.05) either in pasteurization temperature or storage time. It was concluded that pasteurized milk was still suitable for consumption at 15-21 hours storage, while protein concentration tended to be better when was pasteurized at 65oC. Key words: Pasteurized milk, storage qualit
The utilization of rock phosphate (natural defluorinated calcium phosphate or NDCP) in laying hens diet to replace dicalcium phosphate
Get PDFAn experimentwas conducted to study the utilization of local rock phosphate or natural defluorinated calcium phosphate (NDCP) as phosphorus source for layer chickens by using the imported dicalcium phosphate (DCP) as a reference. Eight experimental diets consisted of 2 source of phosphorus (DCP and NDCP) and 4 dietary total P levels (0.4, 0.5, 0.6 and 0.7%) were formulated. Each diet was fed to 24 pullets (6 replicates with 4 birds each) from 20 weeks of age to 14 weeks of egg production. Observations were made on feed consumption, egg production, egg weight, mortality, egg quality, Ca and P retention and ash content of tibial bones . Results showed no significant effect of different source and level of phosphorus tested on egg production (% HD), feed consumption, egg weight and mortality rates . Egg shell thickness was depressed in NDCP diet as compared with DCP, however this only occurred at firstmonth of production. It is concluded that the NDCP can be used in layers diet to replace DCP as phosphorus source. The relative biological value of phosphorus inNDCP is 96% for layers. Keywords: Rock phosphate, NDCP, layers, phosphorou
Protein profiles of field isolates ofBacillus anthracis from different endemic areas of Indonesia
Get PDFSonicated cell-free extract proteins of 14 field isolates ofBacillus anthracis from six different endemic areas of Indonesia were analyzed by the use of sodium dodecyl sulphate-polyacrylamide gel electrophoresis (SDS-PAGE) methods . The protein profiles of each field isolate tested demonstrated slightly different at the protein bands with molecular weights of 18, 37, 52, 65 and 70 kDa, and varied between the field isolates and vaccine strains. The variation could provide clues to the source of anthrax transmission whether it was originated from similar strain or not. Key words : Protein profiles, Bacillus anthracis, SDS-PAG
Crossbreeding between male pelung and female selected native chicken at second generation (G2)
Get PDF The experiment aimed to produce native chicken with faster growth rate that is to achieve body weight of more than 1 kg at 3 months of age. Three hundreds and thirty three crossbreeds (PK) day old chicken from the results of artificial insemination between male Pelung and second generation of selection of female native chicken, were used in this experiment. One hundred and eighty purebreds native chickens were also used as control population. They were put in grower cages with density of 10 birds per cage and each cage was treated as a single unit of replication. Feeds during experiment were given and divided into 3 phases that is Starter Feeds I (Protein 21% and Energy 3000 kcal/kg) for chicken between 0-21 days of age, Starter Feeds II (Protein 19% and Energy 2900 kcal/kg) for chicken between 22-42 days and Grower Feeds (Protein 17% and Energy 2900 kcal/kg) for chicken between 43-84 days. Variables which were recorded were weekly body weight until 12 weeks of age, feed consumption, feed conversion, mortality, carcass weight and simple economic ratio (B/C ratio). The results of the experiment showed that body weight at 12 weeks of crossbred (PK) was significantly higher than the pure native chicken (1000 vs 923 g) (P0.05). Feed consumption was not significantly different (3037 vs 3036 g/bird/12 weeks), but the feed conversion of the crossbred was significantly better than the purebred (3.09 vs 3.4) (P0.05). Carcass weight and carcass components were not significantly different between the two breeds, but they were different between sexes (P0.05). Simple economic calculation showed that crossbreeds produced higher profit than purebreds with the benefit cost ratio were 1.31 for crossbred (PK) and 1.2 for purebred native chicken. The mortality rate during the experiment was low, that is 5.5% for crossbred and 6.36% for purebred. Key words: Crossbreeding, male Pelung, female native chicke