BioWallacea Journal of Biological Research
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Analisis Kualitas Ruang Terbuka Hijau Kebun Raya Kendari
Get PDFRapid urban development to meet the population\u27s needs impacts ecological changes and a decrease in environmental quality. Green Open Space (RTH) is an important element in supporting ecological balance and improving the quality of the urban environment. Although Law No. 26 of 2007 mandates the allocation of 30% of land for green spaces, in reality only about 10% is managed privately and 20% by the public sector. This study aims to evaluate the quality of the 113-hectare Kendari Botanical Garden, Southeast Sulawesi\u27s first conservation space, which is experiencing accessibility and infrastructure challenges despite physical development. The methods used include interviews, observations, and literature studies, with descriptive analysis of accessibility, activities, and facilities aspects. The results showed that the accessibility aspect received a good rating, while the activity and facility aspects were in the fair to good category. This research emphasizes the need for improvement to meet the needs of sustainable urban planning and increase visitor comfort so that Kendari Botanical Garden can function optimally as an inclusive and user-friendly green space
Produksi Biofilm oleh Strain Klebsiella pneumoniae Resistan Antibiotik
Get PDFKlebsiella pneumoniae is known as a Gram-negative bacterium that most often causes infections and is resistant to antibiotics, making it very difficult to treat. Bacteria have several resistance mechanisms, one of which is biofilm production. The purpose of this study was to analyze the biofilm production of Klebsiella pneumoniae strains that are resistant to the antibiotic ciprofloxacin, as a form of defense mechanism. The in vitro research method was used to achieve the goal, carried out by testing clinical specimens of blood, sputum, pus, urine, feces, pleural fluid, with the process of isolation and identification, resistance testing and measuring biofilm production. The tested resistant K. pneumoniae isolates have a resistance mechanism by producing strong, medium and weak qualification biofilms, as well as low porin cell permeability. A strong significant correlation was formed in biofilm production with the amount of bacterial cell growth at the MIC value, which can increase cases of resistance prevalence.
 
Pemanfaatan Tumbuhan oleh Masyarakat Gebang, Teluk Pandan Pesawaran
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Pesawaran Regency, Teluk Pandan District has one of the villages, namely Gebang Village, where the majority of the population are farmers and fishermen. Ethnobotany is a branch of biology that studies the attachment between humans and plants. Ethnobotanical research in Gebang Village has never been done before, this village is unique because it is a coastal area and close to mangrove forests in its area. Therefore, research is needed on the utilization of plants by the people of Gebang Village. The purpose of this study is to record the types of plants in the yard of Gebang Village, the parts of plants that are widely used, utilization, and processing methods. The method used in this research is a semi-structured interview survey method and observation. There are 43 plant species included in 24 families and the family most commonly found and utilized is the Zingiberaceae family. The results of the survey and observation stated that the community utilized yard plants as medicine (22 species), food (21 species), and building materials (5 species). Plant parts that are utilized include leaves (18 types), fruits (17 types), stems (8 types), rhizomes (7 types), flowers (3 types), and roots (1 type). The people of Gebang Village, Pesawaran still utilize plants around their yards for their daily lives
Pengaruh Penambahan Lidah Buaya Pada Pengencer Kuning Telur Dan Madu Terhadap Kualitas Semen Sapi Limousin
Get PDFLidah buaya adalah tanaman yang berbentuk roset dan merupakan tanaman sekulen dengan kandungan senyawa antioksidan termasuk vitamin (A, C, B, E), flavonoid, senyawa fenolik dan polisakarida yang cukup tinggi. Vitamin E memiliki aktivitas antioksidan tertinggi dan berperan sebagai perlindung membran plasma terhadap peroksidasi dari radikal bebas. Penelitian ini bertujuan untuk melihat pengaruh dari pemberian lidah buaya, kuning telur itik dan madu pada kualitas semen cair sapi Limousin yang ditetapkan sebagai bahan alternatif. Pada penelitian ini dilakukan 5 perlakuan dan 4 ulangan yang disebut dengan metode rancangan acak lengkap, beberapa perlakuan serta ulangan terdiri dari P0 : 4 ml kuning telur + 4 ml madu + 15,8 ml aquades, P1 : 1 ml lidah buaya + 4 ml kuning telur + 0,12 ml madu + 14,8 ml aquades, P2 : 0,3 ml lidah buaya + 4 ml kuning telur + 0,12 ml madu + 15,5 aquades dan P3 : 0,5 ml lidah buaya + 4 ml kuning telur + 0,12 ml madu + 15,3 ml aquades. Parameter yang akan diukur yakni viabilitas, motilitas serta abnormalitas spermatozoa pada semen sapi Limousin. Hasil penelitian yang didapatkan yaitu terlihat bahwa pada perlakuan P3 dengan penambahan lidah buaya 0,5 ml mampu mempertahankan motilitas selama penyimpanan 6 jam sebanyak 40%, nilai rataan viabilitas tertinggi selama penyimpanan 9 jam (81,2% ± 4,13c ) dan nilai abnormalitas spermatozoa terendah selama penyimpanan 9 jam (13,5% ± 2,91a ). Kesimpulan dari penelitian ini adalah penambahan lidah buaya sebanyak 0,5 ml sebagai pengencer berpengaruh nyata terhadap kualitas spermatozoa sapi Limousin pada pengencer lidah buaya, kuning telur itik dan madu
Pengaruh Pemberian Auksin dan Giberelin terhadap Pertumbuhan Vegetatif Tanaman Bawang Putih Lokal Timor (Allium sativum L.)
Get PDFLocal Timor garlic has more distinctive taste and aroma and is used as a flavoring and as a medicinal ingredient for people on the island of Timor, especially in Nort Central Timor Regency, but a production has decreased since 2015. Therefore, an efforts to increase production are needed, one of which is by using auxin and gibberellin. This research aims to determine the effect of auxin and gibberellin on the vegetative growth of local Timor garlic. The study used a Completely Randomized Design with 7 treatments, namely P1 (no treatment), P2 (Auxin 50 ppm), P3 (Auxin 100 ppm), P4 (Auxin 150 ppm), P5 (Gibberellin 50 ppm), P6 (Gibberellin 100 ppm), and P7 (Gibberellin 150 ppm). The results showed that the addition of auxin and gibberellin had a significant effect on plant height, especially at 15, 45 and 60 days after planting, but had no significant effect on the number of leaves and stem diameter
Efek Pemberian Monosodium Glutamat (MSG) Terhadap Morfologi Spermatozoa Epididymis Mencit (Mus musculus, L.)
Get PDFThis study aims to determine the effect of giving monosodium glutamate (MSG) on the morphology of epididymal spermatozoa in mice (Mus musculus, L.). A total of 16 male mice weighing 20-30 grams aged 2-3 months were divided into 4 treatments, namely 1 control (PAM water), and 3 treatments of monosodium glutamate, namely; T1 (1.5 ml/g BW), T2 (3.0 ml/g BW), and T3 (4.5 ml/g BW). MSG was administered orally for 34 days. On the 35th day, the mice\u27s body weight was weighed, then dissected and the cauda epididymis was taken to observe spermatozoa morphology using the smear preparation method. Data were analyzed using ANOVA and further HSD Turkey test with a confidence level of 95%. The results showed that administration of monosodium glutamate (MSG) caused abnormal spermatozoa morphology and increased their percentage. The average percentage reduction in normal spermatozoa morphology in all treatments sequentially, namely the dose of 1.5 ml/g BW (T1) was 40.50%, the dose 3.0 ml/g BW (T2) was 55.42%, the dose was 4.5 ml /g BW (T3) was 74.75%, while the decrease was only 11.50% for the control (T0). Based on the research results, it was concluded that administration of monosodium glutamate (MSG) increased the percentage of abnormal epididymal spermatozoa in mice (Mus musculus, L.).
Key words: Monosodium glutamate, spermatozoa morphology, Mus musculus
Abstrak
Penelitian ini bertujuan untuk mengetahui pengaruh pemberian monosodium glutamat (MSG) terhadap morfologi spermatozoa epididimis pada mencit (Mus musculus, L.). Sebanyak 16 ekor mencit jantan dengan berat badan 20-30 gram umur 2-3 bulan dibagi menjadi 4 perlakuan yaitu 1 kontrol (air PAM), dan 3 perlakuan pemberian monosodium gutamat yaitu; P1 (1,5 ml/g BB), P2 (3,0 ml/g BB), dan P3 (4,5 ml/g BB). MSG diberikan secara oral selama 34 hari. Pada hari ke-35 mencit ditimbang berat badannya, kemudian dibedah dan diambil cauda epididimisnya untuk diamati morfologi spermatozoanya dengan metode sediaan apus. Data dianalisis menggunakan ANOVA dan dilanjutkan uji HSD Turkey dengan taraf kepercayaan 95%. Hasil penelitian menunjukkan bahwa pemberian monosodium glutamat (MSG) menyebabkan morfologi spermatozoa abnormal dan meningkatkan persentasenya. Rata-rata persentase penurunan morfologi spermatozoa normal pada semua perlakuan secara berurutan yaitu dosis 1,5 ml/g BB (T1) sebesar 40,50%, dosis 3,0 ml/g BB (T2) sebesar 55,42%, dosis 4,5 ml/g BB (T3) sebesar 74,75%, sedangkan penurunannya hanya sebesar 11,50% pada kontrol (T0). Berdasarkan hasil penelitian dapat disimpulkan bahwa pemberian monosodium glutamat (MSG) dapat meningkatkan persentase spermatozoa epididimis abnormal pada mencit (Mus musculus, L.).
Kata kunci : Monosodium glutamat, morfologi spermatozoa, Mus musculus
Aktivitas Antibakteri Gram Positif Serta Penetapan Kadar Flavonoid dan Fenolik Total Dari Ekstrak Dan Fraksi Daun Jati (Tectona grandis Linn.F.)
Get PDFThe increasing resistance of bacteria to several antibiotics provides a great opportunity to exploit the potential of natural antibiotics from plants. This study aims to determine the antibacterial activity of extracts and fractions of teak leaves (Tectona grandis Linn.F.), as well as determining phenolic and flavonoid levels. Teak leaf samples were extracted using the maceration method with ethanol solvent. The extract was fractionated using n-hexane, chloroform and ethyl acetate solvents. Antibacterial tests were carried out on the bacteria Staphylococcus aureus ATCC 25923, Bacillus subtilis ATCC 21332, and Streptococcus mutans ATCC 25175 using the Broth microdilution method by measuring the MIC and MBC values. The results of the antibacterial activity test of the ethanol extract, ethyl acetate fraction, chloroform fraction and n-hexane fraction against the bacteria Staphylococcus aureus, Bacillus subtilis and Streptococcus mutans obtained an MIC value at a sample concentration of 256 µg/mL. The MBC value of the sample against Staphylococcus aureus was at a concentration of 1024 µg/mL. The total phenolic content of teak leaf extracts and fractions, namely ethanol extract, was 108 mg/g sample, n-hexane fraction 183.556 mg/g sample, chloroform fraction 59.11 mg/g, and ethyl acetate fraction 364 mg/g sample. The total flavonoid content of teak leaf extracts and fractions, namely ethanol extract, was 263.53 mg/g sample, n-hexane fraction 522.353 mg/g sample, chloroform fraction 192.94 mg/g sample, and ethyl acetate fraction 265.49 mg/g. g sample
Kelimpahan Plankton Pada Air Goa Di Kawasan Karst Kecamatan Gu Kabupaten Buton Tengah Sulawesi Tenggara
Get PDFThis study aims to determine the diversity of plankton in Goa water in the Karst area of Gu District, Central Buton Regency, Southeast Sulawesi. This research is an exploratory type of research to determine the diversity of plankton species present in cave water in the Karst area in Gu District, Central Buton Regency, Southeast Sulawesi. The approach used is quantitative description to provide an overview of environmental factors in cave waters and plankton diversity. Plankton sampling was carried out at each station during high tide and low tide. Plankton samples were obtained by filtering cave water using a plankton net. After the filtering process, the sample is put in a sample bottle and preserved using Lugol\u27s. The results of the study found 21 types of plankton belonging to 7 classes namely; Class Bacillariophyceae (9 species), Conjugatophyceae (1 species), Chlorophyceae (4 species), Coscinodiscophyceae (1 species), Copepoda (3 species), Euglenophyceae (1 species) and Chrsophyceae (1 species). The results of the analysis of the plankton diversity index showed that station I had a diversity index of 2.618, then stations II were 2.609 respectively. The plankton diversity index value of all stations like this, it shows that the water quality is relatively good.
Keywords : Plankton Diversity, Water Goa, Karst Region, Central Buton.
ABSTRAK
Penelitian ini bertujuan untuk mengetahui keanekaragaman plankton pada air Goa kawasan Karst Kecamatan Gu Kabupaten Buton Tengah Sulawesi Tenggara. Penelitian ini merupakan jenis penelitian yang bersifat eksploratif untuk mengetahui keanekaragaman jenis plankton yang ada pada air goa kawasan Karst di Kecamatan Gu Kabupaten Buton Tengah Sulawesi Tenggara. Pendekatan yang digunakan yaitu deskripsi kuantitatif untuk memberikan suatu gambaran mengenai faktor-faktor lingkungan perairan goa dan keanekaragaman plankton. Pengambilan sampel plankton dilakukan pada masing-masing stasiun pada saat air pasang dan air surut. Sampel plankton diperoleh dengan menyaring air gua menggunakan plankton net. Setelah proses penyaringan sampel dimasukan dalam botol sampel dan diawetkan menggunakan lugol. Hasil penelitian ditemukan 21 jenis plankton yang tergolong dalam 7 kelas yaitu; Kelas Bacillariophyceae (9 spesies), Conjugatophyceae (1 spesies), Chlorophyceae (4 spesies), Coscinodiscophyceae (1 spesies), Copepoda (3 spesies), Euglenophyceae (1 spesies) dan Chrsophyceae (1 spesies). Hasil analisis indeks keanekaragaman plankton menunjukkan pada stasiun I yang memiliki indeks keanekaragamannya sebesar 2,618, selanjutnya, stasiun II sebesar 2,609. Nilai indeks keanekaragaman plankton menunjukan kualitas perairan tergolong baik.
Kata kunci: Keanekaragaman Plankton, Air Goa, Kawasan Karst, Buton Tengah
Daya Antioksidan Daging Buah Sepuluh Kultivar Pisang Meja Indonesia
Get PDFABSTRACT
Dessert banana that have AA, AAA, and AAB genotypes is one of the groups from the great diversity of bananas. Their nutritional content and antioxidant compounds can be accessed better than cooking banana by the human body. This research aimed to investigate antioxidant potential of the ripe pulp from ten Indonesian dessert banana cultivars. They were Ambon Putih, Ambon Kuning, Ambon Badak, Ambon Lumut, Raja Sereh, Raja Bulu, Lampung, Papan, Rejang, and Udang. Total polyphenol content, total flavonoid content, and IC50 value were respectively determinated using Folin Ciocalteu method, AlCl3 methode, and DPPH method. In addition water content and extract yield percentage also were analyzed. The freeze dried banana pulp was macerated using 96% ethanol for 24 hours then filtered and evaporated using a rotary evaporator. The highest water content and yield percentage were shown respectively by Ambon Badak (AAA) at 73.84% and Papan (AAA) at 68.95% extract rendemen. The highest total polyphenol and flavonoid content ware shown respectively by Rejang (AA) at 3498.63 mg GAE/Kg extract and Ambon Lumut (AAA) at 2756.07 mg QE/Kg extract. IC50 values of banana pulp ranged from 3047.42 mg/L on Rejang (AA) cultivar to 14282.84 mg/L on the Papan cultivar. Rejang is the recommended cultivar for the highest total polyphenol while Ambon Lumut is recommended for the highest flavonoid content. These cultivars are recommended bananas for consumption or for further development.
Kata kunci: antioxidant, polyphenol, flavonoid, IC50, dessert banana
Pemanfaatan Ekstrak Tanaman Gedi (Abelmoschus manihot L.) Asal Kabupaten Wakatobi Sebagai Bahan Aktif Antibakteri Dalam Pembuatan Hand Sanitizer: Pemanfaatan Ekstrak Tanaman Gedi (Abelmoschus manihot L.) Asal Kabupaten Wakatobi Sebagai Bahan Aktif Antibakteri Dalam Pembuatan Hand Sanitizer
No full textThe most important health problem in developing countries, including Indonesia, is infection, which is transmitted by microorganisms. Various types of microorganisms attach to hands every day through physical contact with the environment. Hand sanitizer can be the product of choice for today\u27s society. Hand sanitizers can be made from alcohol, glycerol, trichosan and other chemicals. The alcohol content in the hand sanitizer has bactericidal activity, but excessive use of alcohol can cause irritation to the skin, especially in children. Utilization of extracts from traditional herbal gardens can be an alternative to overcome this. Gedi (Abelmoschus manihot L.) is a plant that is often used as traditional medicine by people in Wakatobi Regency and is known to contain antimicrobial compounds in the form of flavonoids, alkaloids, steroids, and saponins which have been shown to have antibacterial effects. This study uses gedi plant extract as a sample of the active antibacterial ingredient in the manufacture of Hand Sanitizer which will be tested using bacteria Staphylococcus aureus in the UHO FMIPA biomolecule and environmental laboratory to see its antibacterial ability. The results of the organoleptic test showed that the Hand Sanitizer was in the form of a liquid preparation, had a distinctive smell of gedi plants, was green in color and felt cold when used. The results of the inhibition zone test at gedi leaf extract concentrations of 45, 35 and 25%/100 ml hand sanitizer showed that preparations containing 45% extract had the greatest inhibition with an average of 17.97 mm. The weakness of this product is generally found in the specific aroma of gedi leaves which gets more pungent with an increase in the concentration of the extract. So that it is necessary to add other aromas to be able to compete with commercial products made from active alcohol which also use perfume