BioWallacea Journal of Biological Research
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Uji Aktivitas Antihiperurisemia Ekstrak Etanol Daun Hantap (Sterculia oblongata R. Brown) pada Mencit (Mus musculus L.) Hiperurisemia
Get PDFThis study aims to determine the antihyperuricemia activity of the ethanol extract of hantap leaves (Sterculia oblongata R. Brown) in hyperuricemia mice (Mus musculus L.). This research is an experimental research which was arranged based on Completely Randomized Design (CRD). The data obtained were analyzed statistically using ANOVA (analysis of variance) (α = 0.05) and continued with the BNT test with a 95% confidence level. A total of 24 mice were divided into 6 treatments with each treatment consisting of 4 mice. Each treatment was started with standard feeding for 7 days and then the initial blood uric acid levels were measured. Furthermore, all test animals in each treatment (except the first treatment as a control treatment), were given potassium oxonate for 2 days until they reached hyperuricemia conditions, then one treatment was given allopurinol (positive control) and three treatments were given hantap leaf ethanol extract (concentration 1%, 4%, and 8%) for 14 days, then fasted for 1 day and the final uric acid level was measured. The results showed that the ethanol extract of hantap leaves had an effect on reducing uric acid levels in mice. Treatment with 1% extract decreased uric acid levels in mice by 1.125 mg/dL, treatment with 4% extract concentration decreased uric acid levels in mice by 1.075 mg/dL and treatment with 8% extract concentration reduced mice uric acid levels by 0.975 mg/dL.
Keywords: Hyperuricemia, Mice, Hantap Leaves, Potassium Oxonate, Allopurinol.
ABSTRAK
Penelitian ini bertujuan untuk mengetahui aktivitas antihiperurisemia ekstrak etanol daun hantap (Sterculia oblongata R. Brown) pada mencit (Mus musculus L.) hiperurisemia. Penelitian ini merupakan penelitian eksperimental yang disusun berdasarkan Rancangan Acak Lengkap (RAL). Data yang diperoleh dianalisis secara statistik menggunakan ANOVA (analysis of variance) (α = 0,05) dan dilanjutkan dengan uji BNT dengan taraf kepercayaan 95%. Sebanyak 24 ekor mencit dibagi dalam 6 kali perlakuan dengan masing-masing perlakuan terdiri dari 4 ekor mencit. Masing-masing perlakuan diawali dengan pemberian pakan standard selama 7 hari lalu diukur kadar asam urat darah awal. Selanjutnya, semua hewan uji pada setiap perlakukan (kecuali perlakukan pertama sebagai kontrol perlakuan), diberikan kalium oksonat selama 2 hari hingga mencapai kondisi hiperurisemia, selanjutnya satu perlakuan diberi allopurinol (kontrol positif) dan tiga perlakuan diberikan ekstrak etanol daun hantap (konsentrasi 1%, 4%, dan 8%) selama 14 hari, kemudian dipuasakan selama 1 hari dan diukur kadar asam urat akhir. Hasil penelitian menunjukkan bahwa ekstrak etanol daun hantap berpengaruh terhadap penurunan kadar asam urat mencit dengan dengan perlakuan ekstrak 1% menurunan kadar asam urat mencit dari 2,04 mg/mL menjadi 1,125 mg/dL, perlakuan konsentrasi ekstrak 4% menurunan kadar asam urat mencit dari 2,15 mg/dL menjadi 1,075 mg/dL dan perlakuan dengan konsentrasi ekstrak 8% menurunkan kadar asam urat mencit dari 2,175 menjadi 0,975 mg/dL.
Kata Kunci : Hiperurisemia, Mencit, Daun Hantap, Kalium Oksonat, Allopurinol
Studi Sitoksisitas dan Sifat Mekanik Nano Hidroksiapatit Dari Cangkang Kerang Pokea (Batissa violacea var. celebensis, von Martens 1897)
Get PDFSintesis nano hidroksiapatit (NHAp) dan studi sifat mekaniknya telah berhasil dilakukan. NHAp dibuat dengan metode hidrotermal selanjutnya dilakukan variasi suhu kalsinasi pada 300oC, 600oC, 900oC, dan 1200oC untuk mengetahui sifat mekanik NHAp berdasarkan sifat kekeran (hardness), dan kuat tekan. Suhu kalsinasi memiliki pengaruh terhadap nilai kekerasan (hardness) NHAp. Hasil nalisis pada suhu 300 oC memperoleh nilai kekerasan terendah yakni 18,24VHN, sedangkan angka kekerasan optimum yaitu sebesar 36,57VHN pada suhu kalsinasi 1200oC. Nilai kuat tekan optimum berada pada suhu kalsinasi 900oC sebesar 3,12 MPa. Sedangkan pada suhu kalsinasi 1200oC menurun menjadi 0,93 MPa. Penurunan nilai kuat tekan pada suhu 1200oC diakibatkan oleh sifat porous material yang disintesis. Hal ini juga dikonfirmasi dari data SEM terlihat bahwa Pola porous interkoneksi yang teramati pada suhu kalsinasi yang tinggi mengakibatkan dinding nano hidroksiapatit (NHAp) akan berubah menjadi tipis dan jari-jari porous interkoneksi semakin besar sehingga menurunkan sifat mekanik. Hasil uji MTT assay persentase sel hidup optimum diperoleh pada variasi konsentrasi NHAp 30 µg/mL dengan persentase sel hidup sebesar 112,23%. Hal ini membuktikan bahwa NHAp tidak bersifat toksik pada jaringan firoblast sehingga memungkinkan untuk diaplikasi dalam bidang bone grafting
Efek Ekstrak Daun Bayam Brasil (Alternanthera sissoo hort) terhadap Jumlah Limfosit dan Indeks Organ Limfoid Mencit Terinduksi CFA
Get PDFBrazilian spinach (Alternanthera sissoo hort) is a potential plant to be used as an immunomodulator because of its various content of phytochemicals and vitamins, especially vitamin E. This study aimed to determine the potential of Brazilian spinach leaf extract in modulating the number of lymphocytes, as well as lymphoid organ index in mice induced with Complete Freund Adjuvant (CFA). Brazilian spinach leaves were extracted by maceration method in ethanol solvent. Parameters measured from its leaves extract included phytochemical groups, total flavonoid levels and vitamin E. Balb/C male mice were employed as experimental animals to test the effect of Brazilian Spinach leaves extract to mice lymphocytes count, and thymus and spleen index. Mice were acclimatized for 7 days, then induced with CFA via subcutaneous, and given oral treatment for 7 days based on the test group, namely KS (healthy control), KN (negative control), KP (vitamin E 200 IU), and EEBB (spinach leaves extract with doses of 209.25 mg/g BW, 418.4 mg/g BW and 627.72 mg/g BW). The results showed that Brazilian spinach extract contained flavonoids, alkaloids, saponins, steroids, and tannins. Its total flavonoid content was 86.25 mg QE/g and its vitamin E level was 375.5 mg/100 g. In vivo tests showed leaves extract modulating effect on lymphocyte counts and thymus and spleen index. Dose of 627.72 mg/g BW was the best one in providing immunomodulatory effects in the form of lymphocytes number decrease in CFA-induced mice
Efek Bokashi Berbahan Daun Alang-Alang dan Kirinyu Terhadap Pertumbuhan dan Produksi Jagung (Zea mays L.) Pada Tanah Ultisol
Get PDFThe aim of this research is to determine the effect of bokashi made from alang-alang and kirinyu leaves on corn growth in Ultisol soil. This research was carried out from January to March 2022 in Mokoau Village, Kendari City. The research design used was a Randomized Group Design (RAK). The treatment tested was a dose of bokashi mixed with alang-alang and kirinyu leaves consisting of 5 levels, namely B0 (Control) 0 g polybag-1, (B1) 100 g polybag-1, (B2) 200 g polybag-1, (B3) 400 g polybag-1 and (B4) 800 g polybag-1. Each treatment was repeated three times resulting in 15 research units. Variables observed: 1) Initial soil analysis, 2) Analysis of bokashi nutrients, 3) Plant results: (1) Plant height at 14, 28 and 42 days after planting (DAT), (2) Stem diameter at 14, 28 and 42 days after planting (DAT), (3) Number of leaves aged 14, 28 and 42 days after planting (DAP), (4) Diameter of cobs without husks, (5) Length of cobs with husks (6) Length of cobs without husks, (7 ) Weight of cobs with lobs, (8) Weight of cobs without lobs. Conclusions from this research: 1). Providing bokashi with a mixture of alang-alang and kirinyu leaves had a very significant effect on plant height, stem diameter, number of leaves, diameter of cobs without husks, length of cobs with husks, length of cobs without husks, weight of cobs with husks, weight of cobs without husks. 2). A better treatment is treatment (B4) with a dose of 800 g polybag-1.
Keywords: bokashi, alang-alang, kirinyu, corn, ultisol
ABSTRAK
Tujuan penelitian ini adalah mengetahui efek bokashi berbahan daun alang-alang dan kirinyu terhadap pertumbuhan jagung pada tanah Ultisol. Penelitian ini dilaksanakan bulan Januari sampai Maret 2022 di Kelurahan Mokoau, Kota Kendari. Rancangan penelitian yang digunakan adalah Rancangan Acak Kelompok (RAK). Perlakuan yang diuji adalah dosis bokashi campuran daun alang-alang dan kirinyu terdiri 5 taraf yaitu B0 (Kontrol) 0 g polibag-1, (B1) 100 g polibag-1, (B2) 200 g polibag-1, (B3) 400 g polibag-1 dan (B4) 800 g polibag-1. Masing-masing perlakuan diulang tiga kali sehingga menjadi 15 unit penelitian. Variabel yang diamati: 1) Analisis tanah awal, 2) Analisis unsur hara bokashi, 3) Hasil tanaman : (1) Tinggi tanaman umur 14, 28 dan 42 hari setelah tanam (HST), (2) Diameter batang umur 14, 28 dan 42 hari setelah tanam (HST), (3) Jumlah daun umur 14, 28 dan 42 hari setelah tanam (HST), (4) Diameter tonngkol tanpa kelobot, (5) Panjang tongkol berkelobot (6) Panjang tongkol tanpa kelobot, (7) Bobot tongkol berkelobot, (8) Bobot tongkol tanpa kelobot. Kesimpulan dari penelitian ini: 1). Pemberian bokashi campuran daun alang-alang dan kirinyu berpengaruh sangat nyata terhadap tinggi tanaman, diameter batang, jumlah daun, diameter tongkol tanpa kelobot, panjang tongkol berkelobot, panjang tongkol tanpa kelobot, bobot tongkol berkelobot, bobot tongkol tanpa kelobot. 2). Perlakuan yang lebih baik adalah perlakuan (B4) dengan dosis 800 g polibag-1.
Kata kunci: bokashi, alang-alang,kirinyu, jagung, ultiso
Keanekaragaman dan Dominansi Gastropoda di Perairan Pantai Air Salobar Kelurahan Nusaniwe Kecamatan Nusaniwe Kota Ambon
Get PDFoai:ojs2.biowallacea.uho.ac.id:article/3Air Salobar Beach has become a tourist location for domestic and foreign tourists. Apart from having a positive impact, the increasing number of tourists visiting has a negative impact on the sustainability of marine biota such as gastropods. This research aims to determine the types of gastropods, diversity and dominance of these gastropods in the coastal waters of Air Salobar, Nusaniwe Village, Nusaniwe District, Ambon City using the transect method. The research location is divided into 2 stations, each station consists of 6 transects and each transect consists of 5 plots with a plot size of 1 x 1 meter. Based on the research results, 12 species of gastropods were found, namely Nerita polita, Nerita maxima, Nerita plicata, Nerita chamaeleon, Nerita squamulata, Thais aculeate, Morula funiculus, Morula margariticola, Lunella cinerea, Conus ebraeus, Vasum turbinellus and Clypeomorus subbrevacula, with a diversity index (H\u27) at station I is 1.81871, station II is 1.16036 and the average is 1.48953 which shows that the H\u27 diversity value is categorized as medium. Meanwhile, the dominance index (C) at station I is 0.203704, station II is 0.386546 and the average is 0.295125 which shows that no species dominates
Kelimpahan Dan Keanekaragaman Makrozoobentos Sebagai Bioindikator Kualitas Air Sungai Kambu Berdasarkan Penggunaan Lahan Di Kota Kendari
Get PDFThis study aims to determine the index of diversity and abundance of macrozoobetes as a bioindicator of river water quality based on land use in the Kambu River. This research was conducted in November 2022, the sampling technique used the purposive sampling method by dividing three stations where station I was on forest land, station II was on plantation land and station III was on residential land. Based on the results of research conducted at three stations, there were 14 macrozoobenthos families consisting of 8 orders namely Odonata, Hemiptera, Tricoptera, Diptera, Ephemoptera, Decapoda, Haplotaxida, and Sorbeocacha. The highest total abundance value of the three research stations station I produced the highest abundance with a value of 2,778 idm/m2, the highest diversity and uniformity index was at station III with a diversity index value of 1.05 and a uniformity index value of 0.08. While the highest dominance index value with a value of 0.11 is at stations II and III. The results of environmental parameter measurements were carried out, namely physical parameters (temperature) and chemical parameters (pH, DO, BOD, COD and TDS) based on PP No. 22 of 2021 concerning National Water Quality Standards that from the three research stations whose parameters were analyzed, all met quality standard thus the water quality status of the Kambu River is lightly polluted.
Keywords: River, Diversity, Bioindicator, Macrozoobenthos
ABSTRAK
Penelitian ini bertujuan untuk mengetahui indeks keanekaragaman dan kelimpahan makrozoobetos sebagai bioindikator kualitas air sungai berdasarkan penggunaan lahan di Sungai Kambu. Penelitian ini dilaksanakan pada bulan November 2022, teknik sampling menggunakan metode purposive sampling dengan membagi tiga stasiun dimana stasiun I berada pada lahan hutan, stasiun II berada pada lahan perkebunan dan stasiun III berada pada lahan pemukiman. Berdasarkan hasil penelitian yang dilakukan pada tiga stasiun didapatkan makrozoobentos sebanyak 14 family yang terdiri dari 8 Ordo yaitu Odonata, Hemiptera, Tricoptera, Diptera, Ephemoptera, Decapoda, Haplotaxida, dan Sorbeocacha. Total nilai kelimpahan tertinggi dari tiga stasiun penelitian stasiun I menghasilkan kelimpahan tertinggi dengan nilai 2.778 idm/m2, indeks keanekaragaman dan keseragaman tertinggi berada pada stasiun III dengan nilai indeks keanekaragaman 1,05 dan nilai indeks keseragaman 0,08. Sedangkan nilai indeks dominansi tertinggi dengan nilai 0,11 berada pada stasiun II dan III. Hasil pengukuran parameter lingkungan yang dilakukan yaitu parameter fisika (suhu) dan parameter kimia (pH, DO, BOD, COD dan TDS) berdasarkan PP No 22 Tahun 2021 tentang Baku Mutu Air Nasional bahwa dari ke tiga stasiun penelitian yang parameternya dianalisis, semua memenuhi antar baku mutu dengan demikian status mutu air Sungai Kambu tercemar ringan.
Kata Kunci : Sungai, Keanekaragaman, Bioindikator, Makrozoobento
Kadar Senyawa Flavonoid Ekstrak Buah Nyawai (Ficus variegata Blume.) dan Formulanya Sebagai Gel Terhadap Penyembuhan Luka Sayat Mencit (Mus musculus L.)
Get PDFThis study aims to determine the levels of flavonoid compounds and the effectiveness of Nyawai fruit extract gel on wound healing. This study is an experimental study with a completely randomized design (RAL). Using 20 male mice with 5 treatments and 4 replications, namely negative control (without treatment), positive control (Kalmicetine 10%), K1 (gel extract 3%), K2 (gel extract 4%) and K3 (gel extract 5%) with research parameters, namely the length of time for healing and the length of the wound. The results of the test for flavonoid content of Nyawai fruit extract were 11,338 (mg QE/g). The results of the effectiveness of the Nyawai fruit extract gel were statistically processed One Way ANOVA with a 95% confidence level =0.05% followed by the LSD test, the value of the length of time for wound healing was negative control (13 days), K1 (11.75 days) , K2 (11.25 days), K3 (10.75 days) and positive control (9.75 days) while the length of the wound on the skin tissue that closed the fastest was the positive control, K3 (5% extract gel), K2 (gel extract 4%), K1 (gel extract 3%), negative control (without treatment) from the results of the research on the fruit extract gel, it can be concluded that the fruit extract can be used as an alternative in wound healing.
Keywords: Flavonoid, extract, Ficus variegata, gel, wound healing
Abstrak
Penelitian ini bertujuan untuk mengetahui kadar senyawa flavonoid dan efektivitas gel ekstrak buah nyawai terhadap penyembuhan luka sayat. Penelitian ini merupakan penelitian eksperimental dengan rancangan acak lengkap (RAL). Menggunakan 20 ekor mencit jantan sebanyak 5 perlakuan dan 4 ulangan yaitu kontrol negatif tanpa perlakuan (KN), kontrol positif kalmicetin 10% (KP), gel ekstrak 3% (K1), K2 (gel ekstrak 4%) dan K3 (gel ekstrak 5%) dengan parameter penelitian yaitu lama waktu penyembuhan dan panjang luka sayat. Hasil uji kadar flavonoid ekstrak buah nyawai diperoleh 11,338 (mg QE/g). Hasil efektivitas gel ekstrak buah nyawai data diolah secara statistik One Way ANOVA dengan taraf kepercayaan 95% α=0,05% dilanjutkan dengan uji LSD diperoleh nilai lama waktu penyembuhan luka sayat yaitu kontrol negatif (13 hari), K1 (11,75 hari), K2 (11,25 hari), K3 (10,75 hari) dan kontrol positif (9,75 hari) sedangkan panjang luka pada jaringan kulit yang paling cepat menutup yaitu kontrol positif, K3 (gel ekstrak 5%), K2 (gel ekstrak 4%), K1 (gel ekstrak 3%), kontrol negatif (tanpa perlakuan) dari hasil penelitian gel ekstrak buah Nyawai dapat disimpulkan bahwa buah nyawai dapat digunakan sebagai alternatif dalam penyembuhan luka sayat.
Kata Kunci: Flavonoid, ekstrak, Ficus variegata, gel, luka saya