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Characteristics of Sie Reuboh Supplemented with Different Combinations of Palm Vinegar (Arenga pinnata) and Kaffir Lime Leaves (Citrus hystrix)
Get PDFThis research was aimed to analyze the characteristics of sie reuboh processed with the addition of palm vinegar and kaffir lime leaves with different concentrations to produce sie reuboh with a good quality and to maintain the stability of its quality during storage at room temperature. The research consisted of 2 stages. In the first stage, 4000 g of sie reuboh was treated with different doses of palm vinegar and kaffir lime leaves (0 mL and 0 g, 100 mL and 10 g, 120 mL and 20 g, and 140 mL and 30 g) for microbiological analysis. Sie reuboh with the best result of microbiological analysis was later used in Stage 2 to be stored at room temperature and was analyzed for its physical antioxidant activity, thiobarbituric acid reactive substance (TBARS), and microbiological analysis. The result showed that different levels of palm vinegar and kaffir lime leaves did not significantly affect the aw, pH, yield, and microbiological characteristics of the sie reuboh. Different ratios of palm vinegar and kaffir lime leaves significantly affected (P<0.05) the tenderness of sie reuboh. Sie reuboh without addition of palm vinegar and kaffir lime leaves showed a better tenderness than sie reuboh added with palm vinegar and kaffir lime leaves. The best treatment based on microbiological analysis was shown by treatment of sie reuboh with 120 mL of palm vinegar and 20 g of kaffir lime leaves and this sie reuboh was later used to test the stability of the sie reuboh when it was stored at room temperature. The results showed that sie reuboh produced by addition of 120 mL of palm vinegar and 20 g of kaffir lime leaves demonstrated antioxidant activity for inhibition of free radicals as was indicated by the stability of TBARS value during 9 days of storage at room temperature. This combination of treatment could also effectively stabilize the quality of sie reuboh, as indicated by a proper storability at maximum limit of total plate count and mold growth until 3 days of storage at room temperature
Determination of Energy and Protein Requirements of Sheep in Indonesia using a Meta-analytical Approach
Get PDFThe objective of this study was to determine energy and protein requirements, for both maintenance and gain, of sheep in Indonesia by using a meta-analysis method. A database was developed from various in vivo experiments involving sheep as the experimental animals in which energy intake, protein intake and average daily weight gain (ADG) were reported. A total of 38 articles consisting of 137 data points were integrated into the database. Different breeds (Priangan, fat-tailed and local) and sexes (male and female) were specified in the database. Maintenance and gain requirements of dry matter, energy and protein were determined by regressing ADG with dry matter intake (DMI), total digestible nutrient intake (TDNI) and crude protein intake (CPI), respectively. An intercept (where ADG= 0 g/kg MBW/d) and a slope (required nutrient intake per unit ADG) were taken as maintenance and gain requirements, respectively. Results revealed that all sheep breeds had similar energy requirement for maintenance (TDNm). Energy requirement for gain (TDNg) of Priangan breed was lower than other breeds; the breed required 0.860 g TDN for 1 g ADG. Fat-tailed and local breeds required 1.22 and 2.75 g TDN for 1 g ADG, respectively. All breeds also revealed relatively similar protein requirement for maintenance (CPm), i.e. 6.27-6.47 g/kg MBW/d. Priangan breed required less CP for 1 g ADG (CPg), i.e. 0.295 g. Requirements of CPg for fat-tailed and local breeds were 0.336 and 0.497 g/g ADG, respectively. It was concluded that each sheep breed in Indonesia had specific TDN and CP requirements for gain, but similar requirements for maintenance
Isolation and Number of Gonadal Primordial Germ Cells (Gonadal PGCs) on the Stages of Early Embryonic Development of KUB Chicken
Get PDFPrimordial germ cells (PGCs) are cells that will differentiate themselves into spermatogonia in the testis or oogonia in the ovary. Primordial germ cells arise from epiblast and circulate through the bloodstream and finally entering gonadal anlage. The aim of this study was to determine the number of gonadal PGCs of KUB chicken at different development stages. Sixty KUB chicken fertile eggs were divided into four groups (6, 7, 8, and 9 days incubation periods), and incubated at 38 oC with a humidity of 60%. Harvesting was synchronized to the embryonic development at 6-9 d. Gonads were collected using sharp tweezers, and were placed in Eppendorf tube 1.5 mL containing 500 µL PBS [-]. Gonadal PGCs were purified using PBS [-]. The results showed that the average number of gonadal PGCs at 6, 7, 8, and 9 d were 113.7; 143.5; 92.9; and 85.7 cells per embryo, respectively. Number of gonadal PGCs per embryo of KUB chicken were significantly affected by stage of embryonic development (P<0.05), which reached a peak at day 7 of incubation, so that the isolation and collection of PGCs from the gonads were recommended at day 7 of incubation. This information is useful in production of germline chimera of other Indonesian local chickens
Physicochemical Characteristics, in Vitro Fermentation Indicators, Gas Production Kinetics, and Degradability of Solid Herbal Waste as Alternative Feed Source for Ruminants
Get PDFThe aims of this research were to study the nutrient and secondary metabolite contents of solid herbal wastes (SHW) that were preserved by freeze drying, sun drying and silage, as well as to analyze their effects on in vitro fermentation indicators i.e., gas production kinetics and degradability of solid herbal waste. Physical and chemical properties on three forms of SHW (sun dry, freeze dry, and silage) were characterized and then an in vitro gas production experiment was performed to determine the kinetics of gas production, methane production, NH3, microbial protein, and SHW degradability. Polyethylene glycol (PEG) was added to the three treatments to determine the biological activity of tannins. Results showed that all three preparations of SHW still contained high nutrient and plant secondary metabolite contents. Gas production, methane, NH3, microbial protein, in vitro degradability of dry matter (IVDMD) and organic matter (IVDOM) of SHW silage were lower (P<0.05) compared to sun dry and freeze dry. These results were apparently due to the high content of secondary metabolites especially tannin. It can be concluded that solid herbal wastes (SHW) can be used as an alternative feed ingredients for ruminants with attention to the content of secondary metabolites that can affect the process of fermentation and digestibility in the rumen
The Sensory Properties, Color, Microbial, Lipid Oxidation, and Residual Nitrite of Se’i Marinated with Lime and Roselle Calyces Extracts
Get PDFMeat deterioration can occur because of lipid oxidation and bacteria that could affect meat quality. It has been recognized that fruits of lime (Citrus aurantifolia) and roselle (Hibiscus sabdariffa) calyces contain bioactive compounds that have a capability to prevent oxidation and bacterial growth. The objective of this research was to investigate the effect of lime and roselle calyces extracts on se’i (Rotenese smoked beef) quality. Completely randomized design (CRD) with 2x4 factorial pattern was used in this study. The first factor (E) was source of extracts i.e., lime extract (E1) and roselle extract (E2). The second factor (L) was level of the extract consisted of 4 levels i.e., control (without extract/ L0); L1= 1%; L2= 2%; and L3= 3% (v/v). Each treatment consisted of 3 replications. Sensory properties measured were aroma, taste, and tenderness. Other variables measured were color, total plate count (TPC), thiobarbituric acid reactive substances (TBARS), and residual nitrite. The taste and tenderness of se’i were affected (P<0.05) by combination of the extract and the level of the extract. Results showed that there were significant interactions (P<0.05) between the kind of extracts and the level of extract on L (lightness), a (redness), and b (yellowness) values, TPC, TBARS, and residual nitrite values. The level of 3% of lime extract as well as 3% of roselle calyces extract improved score of taste and tenderness, reduced a values, decreased TPC, TBARS, and residual nitrite values. Marinating in 3% of roselle calyces extract decreased the b value but marinating in 3% of lime increased the b value of se’i. It is concluded that marinating 3% of roselle or 3% of lime gives the best effect on taste, tenderness, TPC, and TBARS values of se’i
Effectiveness of the Red Dragon Fruit (Hylocereus polyrhizus) Peel Extract as the Colorant, Antioxidant, and Antimicrobial on Beef Sausage
Get PDFThis study aimed to evaluate the effectiveness of red dragon fruit (Hylocereus polyrhizus) peel extracts addition on beef sausages. Red dragon fruit peel extracts were obtained by maceration using solvent at pH 5. Phytochemical characteristics, total phenols, antioxidant, and antimicrobial activity of the peel extracts were observed. Antioxidant and antimicrobial activities of the extracts were associated with high phytochemical compounds and total phenols contained in the extracts. Red dragon fruit peel extracts with various percentages (0%, 20%, 30%, and 40%) were added on beef sausages, and their physicochemical characteristics, nutrients, antioxidant activity, and microbiological profile were analyzed. The data were analyzed using analysis of variance and Duncan’s multiple range test. Results showed that the addition of red dragon fruit peel extracts significantly reduced texture values, but increased intensity of luminosity, intensity of red color, and intensity of yellow color (P<0.05) beef sausages. It could be concluded that red dragon fruit peel extract containing phytochemical compounds was effective as an antibacterial agent and natural antioxidant. The addition of red dragon fruit peel extracts was effective in increasing the antioxidant activity and decreasing TBARS values. The addition of red dragon fruit peel extract did not affect the reddish colorization of beef sausages, but it was capable of increasing the yellowish colorization on beef sausage
Risk Based Milk Pricing Model at Dairy Farmers Level
Get PDFThe milk price from a cooperative institution to farmer does not fully cover the production cost. Though, dairy farmers encounter various risks and uncertainties in conducting their business. The highest risk in milk supply lies in the activities at the farm. This study was designed to formulate a model for calculating milk price at farmer’s level based on risk. Risks that occur on farms include the risk of cow breeding, sanitation, health care, cattle feed management, milking and milk sales. This research used the location of the farm in West Java region. There were five main stages in the preparation of this model, (1) identification and analysis of influential factors, (2) development of a conceptual model, (3) structural analysis and the amount of production costs, (4) model calculation of production cost with risk factors, and (5) risk based milk pricing model. This research built a relationship between risks on smallholder dairy farms with the production costs to be incurred by the farmers. It was also obtained the formulation of risk adjustment factor calculation for the variable costs of production in dairy cattle farm. The difference in production costs with risk and the total production cost without risk was about 8% to 10%. It could be concluded that the basic price of milk proposed based on the research was around IDR 4,250-IDR 4,350/L for 3 to 4 cows ownership. Increasing farmer income was expected to be obtained by entering the value of this risk in the calculation of production costs.
The Role of Cooperative in The Milk Value Chain in West Bandung Regency, West Java Province
Get PDFThe objective of the study was to evaluate the capacity of Northern Bandung Dairy Farmer Cooperative (KPSBU) to support farmers in producing good quality milk within the milk value chain in West Bandung Regency, West Java. The study used qualitative research strategies including desk study, case study, and focus group discussions. The study compared the involvement of three milk collecting actors that were classified into large cooperative (KPSBU), small cooperative (KUD Puspa Mekar), and private company (Bina Kitri Bersama). A total of 18 dairy farmers, 3 milk collecting actors, 2 milk processors, and one livestock officer were interviewed. Three focus group discussions were conducted. The large cooperative performed better in services, milk collection, and milk quality assessment compared to other milk collecting actors. Members’ commitment, inconsistent milk quality, and shortage of good quality fodder were the hindering factors that influenced the collection of good quality milk in the large cooperative. In conclusion, the large cooperative had better performance in service provision, it also had better infrastructures in milk collection and milk quality assessment compared to other milk collecting actors. Increasing the milk quality standard by milk processing companies affected the milk price since 2000.
Fermentation Characteristics and Nitrogen Retention of Madura Cattle Fed Complete Rations Containing Soybean Pod and By-Products
Get PDFThis study was aimed to evaluate the effect of complete rations containing soybean pod and soybean by-products (soybean meal and tofu waste) on rumen microbial population, fermentation characteristics, nutrient digestibility, and nitrogen retention of Madura cattle. Twelve Madura cattle of 1.5 years of age were given 4 feeding treatments in triplicates in randomized block design experiment. The treatments included T0 (100% native grass) as a negative control, T1 (concentrate: grass (60:40) as a positive control, T2 (complete ration containing 15% soybean pods), and T3 (complete ration containing 30% soybean pods). The treatments were based on feeding practices commonly applied by farmers in the village. The results showed that the use of concentrate rations or complete rations containing soybean pod and by-product did not affect protozoa population, ammonia concentration, and total VFA production compared to cattle fed 100% native grass. In contrast, the use of concentrate rations or complete rations containing soybean pod and by-products reduced acetate and increased butyrate proportion compared to native grass. The use of a concentrate ration resulted the highest propionate proportion. Methane estimation increased with the use of concentrate ration or complete ration containing 15% soybean pod, but it decreased when the level of soybean pod was increased to 30%. It can be concluded that soybean pod has a potential to be used as a fiber source in beef cattle ration to substitute native grass
Isolation of Lactic Acid Bacteria with Cholesterol-Lowering Activity from Digestive Tracts of Indonesian Native Chickens
Get PDFThe aim of the study was to identify the cholesterol-lowering activity of indigenous lactic acid bacteria isolated from the small intestine, cecum, and colon of Indonesian native chickens and evaluated for bile salt hydrolase (BSH) activity in vitro by using MRS media added taurodeoxycholic acid (TDCA) and CaCl2. The quantitative measurement of cholesterol-lowering activity of LAB was investigated by using soluble cholesterol containing MRS broth (100 µg/mL of cholesterin) and incubated at 37 °C for 48 h. Cholesterol content in supernatant was analyzed using microplate reader. The highest percentage of cholesterol reduction found in isolates from colon of native chicken with the value of 17.43% and identified as Lactobacillus plantarum. Based on phylogenetic tree analysis, this isolate was closely related to L. plantarum strain LGFCP4 (accession number KM199683.1) isolated from GIT of Guinea fowl from India. It could be concluded that L. plantarum AKK-30 had cholesterol-lowering activity